LOCUS       RC212617                8424 bp    DNA     circular  SYN 
DEFINITION  ITGA2 (Myc-DDK-tagged)-Human integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) (ITGA2)
ACCESSION   NM_002203
VERSION     NM_002203.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4581..4672 
                     /label="Myc-DDK"  
     misc_feature    4702..5287 
                     /label="PolyA signal"  
     misc_feature    4759..4778 
                     /label="Restriction Enzyme"  
     misc_feature    5563..6235 
                     /label="ColE1"  
     misc_feature    6665..7459 
                     /label="Kan/Neo"  
     misc_feature    7494..7796 
                     /label="SV40 ori"  
     misc_feature    7882..8359 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4563..4569 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4563 
                     /label="ITGA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCAGAA CGGACAGGGG CCGCGCCGCT GCCGCTGCTG CTGGTGTTAG 
     1081 CGCTCAGTCA AGGCATTTTA AATTGTTGTT TGGCCTACAA TGTTGGTCTC CCAGAAGCAA 
     1141 AAATATTTTC CGGTCCTTCA AGTGAACAGT TTGGCTATGC AGTGCAGCAG TTTATAAATC 
     1201 CAAAAGGCAA CTGGTTACTG GTTGGTTCAC CCTGGAGTGG CTTTCCTGAG AACCGAATGG 
     1261 GAGATGTGTA TAAATGTCCT GTTGACCTAT CCACTGCCAC ATGTGAAAAA CTAAATTTGC 
     1321 AAACTTCAAC AAGCATTCCA AATGTTACTG AGATGAAAAC CAACATGAGC CTCGGCTTGA 
     1381 TCCTCACCAG GAACATGGGA ACTGGAGGTT TTCTCACATG TGGTCCTCTG TGGGCACAGC 
     1441 AATGTGGGAA TCAGTATTAC ACAACGGGTG TGTGTTCTGA CATCAGTCCT GATTTTCAGC 
     1501 TCTCAGCCAG CTTCTCACCT GCAACTCAGC CCTGCCCTTC CCTCATAGAT GTTGTGGTTG 
     1561 TGTGTGATGA ATCAAATAGT ATTTATCCTT GGGATGCAGT AAAGAATTTT TTGGAAAAAT 
     1621 TTGTACAAGG CCTGGATATA GGCCCCACAA AGACACAGGT GGGGTTAATT CAGTATGCCA 
     1681 ATAATCCAAG AGTTGTGTTT AACTTGAACA CATATAAAAC CAAAGAAGAA ATGATTGTAG 
     1741 CAACATCCCA GACATCCCAA TATGGTGGGG ACCTCACAAA CACATTTGGA GCAATTCAAT 
     1801 ATGCAAGAAA ATATGCTTAT TCAGCAGCTT CTGGTGGGCG ACGAAGTGCT ACAAAAGTAA 
     1861 TGGTAGTTGT AACTGACGGT GAATCACATG ATGGTTCAAT GTTGAAAGCT GTGATTGATC 
     1921 AATGCAACCA TGACAATATA CTGAGGTTTG GCATAGCAGT TCTTGGGTAC TTAAACAGAA 
     1981 ACGCCCTTGA TACTAAAAAT TTAATAAAAG AAATAAAAGC AATCGCTAGT ATTCCAACAG 
     2041 AAAGATACTT TTTCAATGTG TCTGATGAAG CAGCTCTACT AGAAAAGGCT GGGACATTAG 
     2101 GAGAACAAAT TTTCAGCATT GAAGGTACTG TTCAAGGAGG AGACAACTTT CAGATGGAAA 
     2161 TGTCACAAGT GGGATTCAGT GCAGATTACT CTTCTCAAAA TGATATTCTG ATGCTGGGTG 
     2221 CAGTGGGAGC TTTTGGCTGG AGTGGGACCA TTGTCCAGAA GACATCTCAT GGCCATTTGA 
     2281 TCTTTCCTAA ACAAGCCTTT GACCAAATTC TGCAGGACAG AAATCACAGT TCATATTTAG 
     2341 GTTACTCTGT GGCTGCAATT TCTACTGGAG AAAGCACTCA CTTTGTTGCT GGTGCTCCTC 
     2401 GGGCAAATTA TACCGGCCAG ATAGTGCTAT ATAGTGTGAA TGAGAATGGC AATATCACGG 
     2461 TTATTCAGGC TCACCGAGGT GACCAGATTG GCTCCTATTT TGGTAGTGTG CTGTGTTCAG 
     2521 TTGATGTGGA TAAAGACACC ATTACAGACG TGCTCTTGGT AGGTGCACCA ATGTACATGA 
     2581 GTGACCTAAA GAAAGAGGAA GGAAGAGTCT ACCTGTTTAC TATCAAAGAG GGCATTTTGG 
     2641 GTCAGCACCA ATTTCTTGAA GGCCCCGAGG GCATTGAAAA CACTCGATTT GGTTCAGCAA 
     2701 TTGCAGCTCT TTCAGACATC AACATGGATG GCTTTAATGA TGTGATTGTT GGTTCACCAC 
     2761 TAGAAAATCA GAATTCTGGA GCTGTATACA TTTACAATGG TCATCAGGGC ACTATCCGCA 
     2821 CAAAGTATTC CCAGAAAATC TTGGGATCCG ATGGAGCCTT TAGGAGCCAT CTCCAGTACT 
     2881 TTGGGAGGTC CTTGGATGGC TATGGAGATT TAAATGGGGA TTCCATCACC GATGTGTCTA 
     2941 TTGGTGCCTT TGGACAAGTG GTTCAACTCT GGTCACAAAG TATTGCTGAT GTAGCTATAG 
     3001 AAGCTTCATT CACACCAGAA AAAATCACTT TGGTCAACAA GAATGCTCAG ATAATTCTCA 
     3061 AACTCTGCTT CAGTGCAAAG TTCAGACCTA CTAAGCAAAA CAATCAAGTG GCCATTGTAT 
     3121 ATAACATCAC ACTTGATGCA GATGGATTTT CATCCAGAGT AACCTCCAGG GGGTTATTTA 
     3181 AAGAAAACAA TGAAAGGTGC CTGCAGAAGA ATATGGTAGT AAATCAAGCA CAGAGTTGCC 
     3241 CCGAGCACAT CATTTATATA CAGGAGCCCT CTGATGTTGT CAACTCTTTG GATTTGCGTG 
     3301 TGGACATCAG TCTGGAAAAC CCTGGCACTA GCCCTGCCCT TGAAGCCTAT TCTGAGACTG 
     3361 CCAAGGTCTT CAGTATTCCT TTCCACAAAG ACTGTGGTGA GGACGGACTT TGCATTTCTG 
     3421 ATCTAGTCCT AGATGTCCGA CAAATACCAG CTGCTCAAGA ACAACCCTTT ATTGTCAGCA 
     3481 ACCAAAACAA AAGGTTAACA TTTTCAGTAA CGCTGAAAAA TAAAAGGGAA AGTGCATACA 
     3541 ACACTGGAAT TGTTGTTGAT TTTTCAGAAA ACTTGTTTTT TGCATCATTC TCCCTGCCGG 
     3601 TTGATGGGAC AGAAGTAACA TGCCAGGTGG CTGCATCTCA GAAGTCTGTT GCCTGCGATG 
     3661 TAGGCTACCC TGCTTTAAAG AGAGAACAAC AGGTGACTTT TACTATTAAC TTTGACTTCA 
     3721 ATCTTCAAAA CCTTCAGAAT CAGGCGTCTC TCAGTTTCCA AGCCTTAAGT GAAAGCCAAG 
     3781 AAGAAAACAA GGCTGATAAT TTGGTCAACC TCAAAATTCC TCTCCTGTAT GATGCTGAAA 
     3841 TTCACTTAAC AAGATCTACC AACATAAATT TTTATGAAAT CTCTTCGGAT GGGAATGTTC 
     3901 CTTCAATCGT GCACAGTTTT GAAGATGTTG GTCCAAAATT CATCTTCTCC CTGAAGGTAA 
     3961 CAACAGGAAG TGTTCCAGTA AGCATGGCAA CTGTAATCAT CCACATCCCT CAGTATACCA 
     4021 AAGAAAAGAA CCCACTGATG TACCTAACTG GGGTGCAAAC AGACAAGGCT GGTGACATCA 
     4081 GTTGTAATGC AGATATCAAT CCACTGAAAA TAGGACAAAC ATCTTCTTCT GTATCTTTCA 
     4141 AAAGTGAAAA TTTCAGGCAC ACCAAAGAAT TGAACTGCAG AACTGCTTCC TGTAGTAATG 
     4201 TTACCTGCTG GTTGAAAGAC GTTCACATGA AAGGAGAATA CTTTGTTAAT GTGACTACCA 
     4261 GAATTTGGAA CGGGACTTTT GCATCATCAA CGTTCCAGAC AGTACAGCTA ACGGCAGCTG 
     4321 CAGAAATCAA CACCTATAAC CCTGAGATAT ATGTGATTGA AGATAACACT GTTACGATTC 
     4381 CCCTGATGAT AATGAAACCT GATGAGAAAG CCGAAGTACC AACAGGAGTT ATAATAGGAA 
     4441 GTATAATTGC TGGAATCCTT TTGCTGTTAG CTCTGGTTGC AATTTTATGG AAGCTCGGCT 
     4501 TCTTCAAAAG AAAATATGAA AAGATGACCA AAAATCCAGA TGAGATTGAT GAGACCACAG 
     4561 AGCTCAGTAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4621 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4681 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4741 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4801 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4861 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4921 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4981 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5041 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5101 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5161 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5221 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5281 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5341 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5401 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5461 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5521 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5581 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5641 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5701 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5761 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5821 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5881 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5941 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6001 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6061 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6121 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6181 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6241 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6301 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6361 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6421 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6481 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6541 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6601 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6661 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6721 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6781 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6841 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6901 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6961 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7021 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7081 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7141 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7201 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7261 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7321 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7381 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7441 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7501 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7561 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7621 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7801 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7861 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7921 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7981 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8041 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8101 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8161 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8221 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8281 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8341 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8401 TAACAAAAAT TTAACGCGAA TTTT
//