LOCUS       RC212486                8292 bp    DNA     circular  SYN 
DEFINITION  SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 1
ACCESSION   NM_173156
VERSION     NM_173156.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="SMG7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTGCAG AGCGCGCAGT ACCTCCGGCA GGCAGAAGTC CTGAAGGCTG 
     1081 ACATGACAGA TTCTAAGCTG GGTCCAGCTG AAGTCTGGAC ATCCAGGCAG GCTCTGCAGG 
     1141 ACCTGTACCA GAAAATGCTA GTTACCGATT TGGAATACGC TTTAGACAAG AAAGTAGAAC 
     1201 AGGATCTCTG GAATCACGCC TTTAAGAATC AGATCACAAC ACTGCAAGGC CAGGCAAAGA 
     1261 ATCGAGCAAA TCCGAATCGG AGTGAAGTTC AGGCAAACCT TTCTCTGTTC CTAGAGGCAG 
     1321 CTAGTGGCTT CTATACTCAG TTATTACAAG AACTGTGTAC AGTATTTAAT GTAGATTTAC 
     1381 CATGCCGTGT GAAGTCTTCC CAATTGGGAA TTATCAGCAA TAAACAGACG CATACCAGCG 
     1441 CCATAGTGAA GCCACAGTCT AGCTCCTGTT CCTATATCTG CCAGCACTGC CTCGTCCACC 
     1501 TTGGAGACAT TGCTCGATAC AGAAACCAGA CCAGCCAGGC AGAGTCCTAC TATAGGCATG 
     1561 CAGCTCAGCT TGTCCCCTCC AATGGTCAGC CTTATAATCA GTTGGCTATC TTAGCTTCTT 
     1621 CCAAAGGAGA CCATCTGACC ACAATTTTCT ACTACTGCAG AAGCATTGCT GTGAAGTTCC 
     1681 CTTTCCCAGC TGCCTCCACT AATCTGCAAA AAGCACTTTC TAAAGCACTG GAAAGCCGAG 
     1741 ATGAGGTGAA AACCAAGTGG GGTGTTTCTG ACTTCATCAA GGCCTTTATT AAATTCCACG 
     1801 GTCATGTGTA CCTGAGTAAG AGCTTGGAAA AGTTGAGCCC TCTTCGAGAG AAATTGGAAG 
     1861 AACAGTTTAA GAGGCTGCTA TTCCAAAAAG CTTTCAACTC TCAGCAGTTA GTTCATGTCA 
     1921 CTGTCATTAA CCTGTTTCAA CTTCATCACC TTCGTGACTT TAGCAATGAA ACCGAGCAGC 
     1981 ACACTTATAG CCAAGATGAG CAGCTATGTT GGACACAGTT GCTGGCCCTC TTTATGTCTT 
     2041 TTCTTGGCAT CCTGTGCAAG TGTCCTCTAC AGAATGAGTC TCAGGAGGAG TCCTACAATG 
     2101 CCTATCCTCT TCCAGCAGTC AAGGTCTCCA TGGACTGGCT AAGACTCAGA CCCAGGGTCT 
     2161 TTCAGGAGGC AGTGGTGGAT GAAAGACAGT ACATTTGGCC CTGGTTGATT TCTCTTCTGA 
     2221 ATAGTTTCCA TCCCCATGAA GAGGACCTCT CAAGTATTAG TGCGACACCA CTTCCAGAGG 
     2281 AGTTTGAATT ACAAGGATTT TTGGCATTGA GACCTTCTTT CAGGAACTTG GATTTTTCCA 
     2341 AAGGTCACCA GGGTATTACA GGGGACAAAG AAGGCCAGCA ACGACGAATA CGACAGCAAC 
     2401 GCTTGATCTC TATAGGCAAA TGGATTGCTG ATAATCAGCC AAGGCTGATT CAGTGTGAAA 
     2461 ATGAGGTAGG GAAATTGTTG TTTATCACAG AAATCCCAGA ATTAATACTG GAAGACCCCA 
     2521 GTGAAGCCAA AGAGAACCTC ATTCTGCAAG AAACATCTGT GATAGAGTCG CTGGCTGCAG 
     2581 ATGGGAGCCC AGGGCTAAAA TCAGTGCTAT CTACAAGCCG AAATTTAAGC AACAACTGTG 
     2641 ACACAGGAGA GAAGCCAGTG GTTACCTTCA AAGAAAACAT TAAGACACGA GAAGTGAACA 
     2701 GAGACCAAGG AAGAAGTTTT CCTCCCAAAG AGGTGAGAAG GGACTATAGC AAAGGAATAA 
     2761 CTGTAACTAA GAATGATGGA AAGAAGGACA ACAACAAGAG GAAAACTGAA ACCAAGAAAT 
     2821 GCACCTTAGA AAAGTTACAG GAAACAGGAA AGCAGAATGT GGCAGTGCAG GTAAAATCCC 
     2881 AGACAGAACT AAGAAAGACT CCAGTGTCTG AAGCCAGAAA AACACCTGTA ACTCAAACCC 
     2941 CAACTCAAGC AAGTAACTCC CAGTTCATCC CCATTCATCA CCCTGGAGCC TTCCCTCCTC 
     3001 TTCCCAGCAG GCCAGGGTTT CCGCCCCCAA CATATGTTAT CCCCCCGCCT GTGGCATTTT 
     3061 CTATGGGCTC AGGTTACACC TTCCCAGCTG GTGTTTCTGT CCCAGGAACC TTTCTTCAGC 
     3121 CTACAGCTCA CTCTCCAGCA GGAAACCAGG TGCAAGCTGG GAAACAGTCC CACATTCCTT 
     3181 ACAGCCAGCA ACGGCCCTCT GGACCAGGGC CAATGAACCA GGGACCTCAA CAATCACAGC 
     3241 CACCTTCCCA GCAACCCCTT ACATCTTTAC CAGCTCAGCC AACAGCACAG TCTACAAGCC 
     3301 AGCTGCAGGT TCAAGCTCTA ACTCAGCAAC AACAATCCCC TACAAAAGCT GTGCCGGCTT 
     3361 TGGGGAAAAG CCCGCCTCAC CACTCTGGAT TCCAGCAGTA TCAACAGGCA GATGCCTCCA 
     3421 AACAGCTGTG GAATCCCCCT CAGGTTCAAG GCCCATTAGG GAAAATTATG CCTGTGAAAC 
     3481 AGCCCTACTA CCTTCAGACC CAAGACCCCA TAAAACTGTT TGAGCCGTCA TTGCAACCTC 
     3541 CTGTAATGCA GCAGCAGCCT CTAGAAAAAA AAATGAAGCC TTTTCCCATG GAGCCATATA 
     3601 ACCATAATCC CTCAGAAGTC AAGGTCCCAG AATTCTACTG GGATTCTTCC TACAGCATGG 
     3661 CTGATAACAG ATCTGTAATG GCACAGCAAG CAAACATAGA CCGCAGGGGC AAACGGTCAC 
     3721 CAGGAGTCTT CCGTCCAGAG CAGGATCCTG TACCCAGAAT GCCGTTTGAG AAATCCTTAT 
     3781 TGGAGAAGCC CTCAGAGCTC ATGTCACATT CATCCTCTTT CCTGTCCCTC ACCGGATTCT 
     3841 CTCTCAATCA GGAAAGATAC CCAAATAATA GTATGTTCAA TGAGGTATAT GGGAAAAACC 
     3901 TGACATCCAG CTCCAAAGCA GAACTCAGTC CCTCAATGGC CCCCCAGGAA ACATCTCTGT 
     3961 ATTCCCTTTT TGAAGGGACT CCGTGGTCTC CATCACTTCC TGCCAGTTCA GATCATTCAA 
     4021 CACCAGCCAG CCAGTCTCCT CATTCCTCTA ACCCAAGCAG CCTACCCAGC TCTCCTCCAA 
     4081 CACACAACCA TAATTCTGTT CCATTCTCCA ATTTTGGACC CATTGGGACT CCAGATAACA 
     4141 GGGATAGAAG GACTGCAGAT CGGTGGAAAA CTGATAAGCC AGCCATGGGT GGGTTTGGCA 
     4201 TTGATTATCT CTCAGCAACG TCATCCTCTG AGAGCAGTTG GCATCAGGCC AGCACTCCGA 
     4261 GTGGCACCTG GACAGGCCAT GGCCCTTCCA TGGAGGATTC CTCTGCTGTC CTCATGGAAA 
     4321 GCCTAAAGTC TATCTGGTCC AGTTCCATGA TGCATCCTGG ACCTTCTGCT CTGGAGCAGC 
     4381 TGTTAATGCA GCAGAAGCAG AAACAGCAAC GGGGACAAGG CACCATGAAC CCTCCACACA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//