LOCUS       RC212440                7614 bp    DNA     circular  SYN 
DEFINITION  AGAP3 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1
ACCESSION   NM_031946
VERSION     NM_031946.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3771..3862 
                     /label="Myc-DDK"  
     misc_feature    3892..4477 
                     /label="PolyA signal"  
     misc_feature    3949..3968 
                     /label="Restriction Enzyme"  
     misc_feature    4753..5425 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Kan/Neo"  
     misc_feature    6684..6986 
                     /label="SV40 ori"  
     misc_feature    7072..7549 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="AGAP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTTCCAG GCGGGCGGGG GGCAGAGCCC GCAGCAGCAG CAGAGCCTGG 
     1081 CGGCTCCGGG GGGCGGCGGC GCTGCCGCGC AGCAGCTCGT CTGCGGCGGG CAGTTCGGCG 
     1141 GCGCGGGGCC CGGGGCCGGG GGCGGCGGCG GCCCCTCGCA GCAGCTGGCC GGCGGGCCCC 
     1201 CCCAGCAGTT CGCGCTCTCC AACTCCGCGG CCATCCGGGC CGAGATCCAG CGCTTCGAGT 
     1261 CCGTGCATCC CAATATCTAC GCCATCTACG ACCTGATCGA GCGCATCGAG GATTTGGCGC 
     1321 TGCAGAACCA GATCCGGGAG CACGTCATCT CCATCGAGGA CTCGTTTGTG AACAGCCAGG 
     1381 AGTGGACGCT GAGCCGCTCC GTACCGGAGC TTAAAGTGGG CATAGTGGGG AACCTGTCTA 
     1441 GCGGGAAGTC AGCCCTGGTG CACCGCTATC TGACGGGGAC CTATGTCCAG GAGGAGTCCC 
     1501 CTGAAGGGGG GCGGTTTAAG AAGGAGATTG TGGTGGATGG CCAGAGTTAC CTGCTGCTGA 
     1561 TCCGAGATGA AGGAGGCCCC CCTGAGCTCC AGTTTGCTGC CTGGGTGGAT GCAGTGGTGT 
     1621 TTGTGTTCAG CCTGGAGGAT GAAATCAGTT TCCAGACGGT GTACAACTAC TTCCTGCGTC 
     1681 TCTGCAGCTT CCGCAACGCC AGCGAGGTGC CCATGGTGCT TGTGGGCACG CAGGATGCCA 
     1741 TCAGCGCTGC GAATCCCCGG GTTATCGACG ACAGCAGAGC CCGCAAGCTC TCCACAGATC 
     1801 TGAAGCGGTG CACCTACTAT GAGACGTGCG CGACCTACGG GCTCAATGTG GAGCGTGTCT 
     1861 TCCAGGACGT GGCCCAGAAG GTAGTGGCCT TGCGAAAGAA GCAGCAACTG GCCATCGGGC 
     1921 CCTGCAAGTC ACTGCCCAAC TCGCCCAGCC ACTCGGCCGT GTCCGCCGCC TCCATCCCGG 
     1981 CCGTGCACAT CAACCAGGCC ACGAATGGCG GCGGCAGCGC CTTCAGCGAC TACTCGTCCT 
     2041 CAGTCCCCTC CACCCCCAGC ATCAGCCAGC GGGAGCTGCG CATCGAGACC ATCGCTGCCT 
     2101 CCTCCACCCC CACACCCATC CGAAAGCAGT CCAAGCGGCG CTCCAACATC TTCACGTCTC 
     2161 GGAAGGGTGC TGACCTGGAC CGGGAGAAGA AGGCTGCCGA GTGCAAGGTG GACAGCATCG 
     2221 GGAGCGGCCG CGCCATCCCC ATCAAGCAGG GGATCCTGCT AAAGCGGAGC GGCAAGTCCC 
     2281 TGAACAAGGA GTGGAAGAAG AAGTATGTGA CGCTCTGTGA CAACGGGCTG CTCACCTATC 
     2341 ACCCCAGCCT GCATGATTAC ATGCAGAACA TCCACGGCAA GGAGATTGAC CTGCTGCGGA 
     2401 CAACGGTGAA AGTGCCAGGG AAGCGCCTGC CCCGAGCCAC ACCTGCCACA GCCCCGGGCA 
     2461 CCAGCCCCCG TGCCAACGGG CTGTCCGTGG AGCGGAGTAA CACACAGCTG GGTGGGGGCA 
     2521 CAGGTGCCCC CCACTCGGCC AGCAGCGCAT CCCTGCACTC TGAGCGCCCC CTCAGCAGCT 
     2581 CGGCCTGGGC TGGCCCGCGC CCTGAGGGGC TGCACCAGCG CTCCTGCTCC GTTTCCAGCG 
     2641 CCGACCAGTG GAGTGAGGCC ACCACTTCCC TGCCCCCAGG CATGCAGCAC CCTGCCAGTG 
     2701 GCCCAGCTGA GGTACTCAGT TCCAGCCCCA AGCTGGATCC TCCCCCATCT CCCCACTCCA 
     2761 ACCGGAAGAA GCACCGGAGG AAAAAGAGCA CCGGGACCCC CCGACCAGAC GGCCCCAGCA 
     2821 GTGCTACTGA AGAGGCAGAG GAGTCGTTTG AATTTGTGGT GGTGTCCCTC ACTGGGCAGA 
     2881 CGTGGCACTT CGAGGCTTCA ACGGCGGAGG AGCGGGAGCT GTGGGTTCAG AGTGTGCAGG 
     2941 CCCAGATCCT TGCCAGCCTG CAAGGCTGCC GCAGTGCCAA GGACAAGACT CGACTGGGGA 
     3001 ACCAGAACGC AGCTCTGGCT GTGCAGGCCG TCCGCACCGT CCGCGGCAAC AGCTTTTGTA 
     3061 TCGACTGCGA TGCACCCAAT CCAGACTGGG CCAGCCTGAA CCTGGGTGCC CTGATGTGCA 
     3121 TTGAGTGCTC AGGCATCCAC CGACACCTGG GGGCTCACCT GTCCCGGGTG CGCTCCCTTG 
     3181 ACCTCGATGA CTGGCCGCCT GAGCTGCTGG CTGTCATGAC TGCCATGGGC AATGCCCTCG 
     3241 CCAACAGCGT CTGGGAGGGG GCCTTGGGTG GCTACTCCAA GCCAGGGCCT GATGCCTGCA 
     3301 GAGAGGAGAA GGAACGCTGG ATACGGGCCA AGTATGAACA GAAGCTCTTC CTGGCCCCAC 
     3361 TGCCAAGCTC AGATGTGCCA CTGGGGCAGC AGCTGCTCCG GGCCGTGGTG GAAGATGACC 
     3421 TGCGGCTGTT GGTGATGCTC CTGGCACATG GCTCCAAAGA GGAGGTGAAT GAGACCTATG 
     3481 GGGACGGGGA CGGGCGGACG GCTCTACATC TCTCCAGTGC CATGGCCAAC GTTGTCTTCA 
     3541 CGCAGCTGCT CATCTGGTAC GGGGTGGACG TGAGGAGCCG GGACGCCCGG GGCCTGACTC 
     3601 CACTGGCATA TGCTCGCCGG GCCGGCAGCC AGGAGTGTGC AGACATCTTG ATCCAGCATG 
     3661 GCTGCCCTGG GGAGGGCTGT GGCTTAGCGC CTACCCCCAA CAGAGAGCCT GCCAATGGCA 
     3721 CCAACCCCTC TGCTGAGCTG CACCGTAGTC CTAGCCTCCT AACGCGTACG CGGCCGCTCG 
     3781 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3841 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3901 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3961 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4021 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4081 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4141 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4201 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4261 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4321 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4381 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4441 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4501 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4561 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4621 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5341 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5401 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5461 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5521 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5581 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5641 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5701 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5761 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5821 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5881 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5941 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6001 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6061 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6121 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6181 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6241 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6301 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6361 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6421 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6481 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6541 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6601 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6661 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6721 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6781 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6841 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7021 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7081 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7141 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7201 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7261 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7321 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7381 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7441 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7501 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7561 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//