LOCUS RC212413 8895 bp DNA circular SYN DEFINITION IRS2 (Myc-DDK-tagged)-Human insulin receptor substrate 2 (IRS2) ACCESSION NM_003749 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 5052..5143 /label="Myc-DDK" misc_feature 5173..5758 /label="PolyA signal" misc_feature 5230..5249 /label="Restriction Enzyme" misc_feature 6034..6706 /label="ColE1" misc_feature 7136..7930 /label="Kan/Neo" misc_feature 7965..8267 /label="SV40 ori" misc_feature 8353..8830 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 5034..5040 /label="Restriction Enzyme" misc_feature 1020..5034 /label="IRS2" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCGAGCCCG CCGCGGCACG GGCCGCCCGG GCCGGCGAGC GGAGACGGCC 1081 CCAACCTCAA CAACAACAAC AACAACAACA ACCACAGCGT GCGCAAGTGC GGCTACCTGC 1141 GCAAGCAGAA GCATGGCCAC AAGCGCTTCT TCGTGCTGCG CGGACCCGGC GCGGGCGGCG 1201 ACGAGGCGAC GGCGGGCGGG GGGTCGGCGC CGCAACCGCC GCGGCTCGAG TACTACGAGA 1261 GCGAGAAAAA GTGGCGGAGC AAGGCAGGCG CGCCGAAACG GGTGATCGCT CTCGACTGCT 1321 GCCTGAACAT CAACAAGCGC GCCGACGCCA AGCACAAGTA CCTGATCGCC CTCTACACCA 1381 AGGACGAGTA CTTCGCCGTG GCCGCCGAGA ACGAGCAGGA GCAGGAGGGC TGGTACCGCG 1441 CGCTCACCGA CCTGGTCAGC GAGGGCCGCG CGGCCGCCGG AGACGCGCCC CCCGCCGCCG 1501 CGCCCGCCGC GTCCTGCAGC GCCTCCCTGC CCGGCGCCCT GGGCGGCTCT GCCGGCGCCG 1561 CCGGGGCCGA GGACAGCTAC GGGCTGGTGG CTCCCGCCAC GGCCGCCTAC CGTGAGGTGT 1621 GGCAGGTGAA CCTGAAGCCC AAGGGTCTGG GCCAGAGCAA GAACCTGACG GGGGTGTACC 1681 GTCTGTGCCT GTCTGCGCGC ACCATCGGCT TCGTGAAGCT CAACTGCGAG CAGCCGTCGG 1741 TGACGCTGCA GCTCATGAAC ATCCGCCGCT GCGGCCACTC GGACAGCTTC TTCTTCATCG 1801 AGGTGGGCCG CTCGGCCGTC ACAGGCCCCG GCGAGCTGTG GATGCAGGCG GACGACTCGG 1861 TGGTGGCGCA GAACATCCAC GAGACCATCC TGGAGGCCAT GAAGGCGCTC AAGGAGCTCT 1921 TCGAGTTCCG GCCGCGCAGT AAGAGCCAAT CGTCGGGGTC GTCGGCCACG CACCCCATCA 1981 GCGTCCCCGG CGCGCGCCGC CACCACCACC TGGTCAACCT GCCCCCCAGC CAGACGGGCC 2041 TGGTGCGCCG CTCGCGCACC GACAGCCTGG CCGCCACCCC GCCGGCGGCC AAGTGCAGCT 2101 CGTGCCGGGT GCGCACCGCC AGCGAGGGCG ACGGCGGCGC GGCGGCGGGA GCGGCGGCCG 2161 CGGGCGCCAG GCCGGTGTCG GTGGCTGGGA GCCCCCTGAG CCCCGGGCCG GTGCGCGCGC 2221 CCCTGAGCCG CTCGCACACC CTGAGCGGCG GCTGCGGCGG CCGCGGGAGC AAGGTGGCGC 2281 TGCTGCCGGC AGGGGGCGCG CTGCAACACA GCCGCTCCAT GTCCATGCCC GTGGCGCACT 2341 CGCCGCCCGC CGCCACCAGC CCCGGCTCCC TGTCGTCCAG CAGCGGCCAC GGCTCGGGCT 2401 CCTACCCGCC GCCGCCCGGC CCGCACCCGC CTCTGCCGCA TCCGCTGCAC CACGGCCCCG 2461 GCCAGCGGCC CTCCAGCGGC AGCGCCTCCG CCTCGGGCTC CCCCAGCGAC CCCGGCTTCA 2521 TGTCCCTGGA CGAGTACGGC TCCAGCCCAG GCGACCTGCG CGCCTTCTGC AGCCACCGAA 2581 GCAACACGCC CGAGTCCATC GCGGAGACGC CCCCGGCCCG AGACGGCGGC GGCGGCGGTG 2641 AGTTCTACGG GTACATGACC ATGGACAGGC CCCTGAGCCA CTGTGGCCGC TCCTACCGCC 2701 GGGTCTCGGG GGACGCGGCC CAGGACCTGG ACCGAGGGCT GCGCAAGAGG ACCTACTCCC 2761 TGACCACGCC AGCCCGGCAG CGGCCGGTGC CCCAGCCCTC CTCTGCCTCG CTGGATGAAT 2821 ACACCCTGAT GCGGGCCACC TTCTCGGGCA GCGCGGGCCG CCTCTGCCCG TCCTGCCCCG 2881 CGTCCTCTCC CAAGGTGGCC TACCACCCCT ACCCAGAGGA CTACGGAGAC ATCGAGATCG 2941 GCTCCCACAG GAGCTCCAGC AGCAACCTGG GGGCAGACGA CGGCTACATG CCCATGACGC 3001 CCGGCGCGGC CCTCGCGGGC AGTGGGAGCG GCAGCTGCAG GAGCGACGAC TACATGCCCA 3061 TGAGCCCCGC CAGCGTGTCC GCCCCCAAGC AGATCTTGCA GCCCAGGGCC GCCGCCGCCG 3121 CCGCCGCCGC CGTGCCTTCT GCGGGGCCTG CGGGGCCAGC ACCCACCTCT GCGGCGGGCA 3181 GGACATTCCC GGCGAGCGGG GGCGGCTACA AGGCCAGCTC GCCCGCCGAG AGCTCCCCCG 3241 AGGACAGTGG GTACATGCGC ATGTGGTGCG GTTCCAAGCT GTCCATGGAG CATGCAGATG 3301 GCAAGCTGCT GCCCAACGGG GACTACCTCA ACGTGTCCCC CAGCGACGCG GTCACCACGG 3361 GCACCCCGCC CGACTTCTTC TCCGCAGCCC TGCACCCCGG CGGGGAGCCG CTCAGGGGCG 3421 TTCCCGGCTG CTGCTACAGC TCCTTGCCCC GCTCCTACAA GGCCCCCTAC ACCTGTGGCG 3481 GGGACAGCGA CCAGTACGTG CTCATGAGCT CCCCTGTGGG GCGCATCCTG GAGGAGGAGC 3541 GTCTGGAGCC TCAGGCCACG CCAGGGCCCA GCCAGGCGGC CAGCGCCTTC GGGGCCGGCC 3601 CCACGCAGCC CCCTCACCCT GTAGTGCCTT CGCCCGTGCG GCCTAGCGGC GGCAGCCCGG 3661 AGGGCTTCTT GGGCCAGCGC GGCCGGGCGG TGAGGCCCAC GCGCCTGTCC CTGGAGGGGC 3721 TGCCCAGCCT GCCCAGCATG CACGAGTACC CACTGCCACC GGAGCCCAAG AGCCCCGGCG 3781 AGTACATCAA CATCGACTTT GGCGAGCCCG GGGCCCGCCT GTCGCCGCCC GCGCCTCCCC 3841 TGCTGGCGTC GGCGGCCTCG TCCTCCTCGC TCTTGTCCGC CAGCAGCCCG GCCTCGTCGC 3901 TGGGCTCAGG CACCCCGGGC ACCAGCAGCG ACAGCCGGCA GCGGTCTCCG CTCTCCGACT 3961 ACATGAACCT CGACTTCAGC TCCCCCAAGT CTCCTAAGCC GGGCGCCCCG AGCGGCCACC 4021 CCGTGGGCTC CTTGGACGGC CTCCTGTCCC CCGAGGCCTC CTCCCCGTAT CCGCCGTTGC 4081 CCCCGCGTCC GTCCGCGTCC CCGTCGTCGT CTCTGCAGCC GCCGCCACCG CCGCCGGCCC 4141 CGGGGGAGCT GTACCGCCTG CCCCCCGCCT CGGCCGTTGC CACCGCCCAG GGCCCGGGCG 4201 CCGCCTCATC GTTGTCCTCG GACACCGGGG ACAATGGTGA CTACACCGAG ATGGCTTTTG 4261 GTGTGGCCGC CACCCCGCCG CAACCTATCG CGGCCCCCCC GAAGCCAGAA GCTGCCCGCG 4321 TGGCCAGCCC GACGTCGGGC GTGAAGAGGC TGAGCCTCAT GGAGCAGGTG TCGGGAGTCG 4381 AGGCCTTCCT GCAGGCCAGC CAGCCCCCGG ACCCCCACCG CGGCGCCAAG GTCATCCGCG 4441 CAGACCCGCA GGGGGGCCGC CGCCGCCACA GTTCCGAGAC CTTCTCCTCC ACCACGACGG 4501 TCACCCCCGT GTCCCCGTCC TTCGCCCACA ACCCCAAGCG CCACAACTCG GCCTCCGTGG 4561 AAAATGTCTC TCTCAGGAAA AGCAGCGAGG GCGGCGTGGG TGTCGGCCCT GGAGGGGGCG 4621 ACGAGCCGCC CACCTCCCCA CGACAGTTGC AGCCGGCGCC CCCTTTGGCA CCGCAGGGCC 4681 GGCCGTGGAC CCCGGGTCAG CCCGGGGGCT TGGTCGGTTG TCCTGGGAGC GGTGGATCGC 4741 CCATGCGCAG AGAGACCTCT GCCGGCTTCC AGAATGGTCT CAACTACATC GCCATCGACG 4801 TGAGGGAGGA GCCCGGGCTG CCACCCCAGC CGCAGCCGCC GCCGCCGCCG CTTCCTCAGC 4861 CGGGAGACAA GAGCTCCTGG GGCCGGACCC GAAGCCTCGG GGGTCTCATC AGCGCTGTGG 4921 GCGTCGGCAG CACCGGCGGC GGGTGCGGGG GGCCGGGTCC CGGTGCCCTG CCCCCTGCCA 4981 ACACCTACGC CAGCATTGAC TTCTTGTCCC ACCACTTGAA GGAGGCCACC ATCGTGAAAG 5041 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 5101 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 5161 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 5221 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 5281 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 5341 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 5401 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 5461 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 5521 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 5581 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 5641 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 5701 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 5761 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 5821 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 5881 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 5941 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 6001 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 6061 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 6121 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 6181 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 6241 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 6301 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 6361 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 6421 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 6481 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 6541 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 6601 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 6661 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 6721 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 6781 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 6841 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 6901 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 6961 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 7021 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 7081 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 7141 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 7201 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 7261 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 7321 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 7381 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 7441 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 7501 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 7561 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 7621 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 7681 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 7741 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 7801 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 7861 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 7921 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 7981 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 8041 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 8101 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 8281 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 8341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 8401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 8461 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 8521 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 8581 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 8641 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 8701 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 8761 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 8821 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 8881 TTTAACGCGA ATTTT //