LOCUS       RC212274L1                9030 bp    DNA     circular  SYN 
DEFINITION  SP100 (Myc-DDK-tagged)-Human SP100 nuclear antigen (SP100), transcript variant 1
ACCESSION   NM_001080391
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7321..7501 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8229..8888 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7660..8228 
                     /label="ColE1"  
     misc_feature    6196..6216 
                     /label="LR50 primer"  
     misc_feature    5993..6064 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6205..6796 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5975..5981 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5975 
                     /label="SP100"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCAGGTGGG GGCGGCGACC TGAGCACCAG 
     3361 GAGGCTGAAT GAATGTATTT CACCAGTAGC AAATGAGATG AACCATCTTC CTGCACACAG 
     3421 CCACGATTTG CAAAGGATGT TCACGGAAGA CCAGGGTGTA GATGACAGGC TGCTCTATGA 
     3481 CATTGTATTC AAGCACTTCA AAAGAAATAA GGTGGAGATT TCAAATGCAA TAAAAAAGAC 
     3541 ATTTCCATTC CTCGAGGGCC TCCGTGATCG TGATCTCATC ACAAATAAAA TGTTTGAAGA 
     3601 TTCTCAAGAT TCTTGTAGAA ACCTGGTCCC TGTACAGAGA GTGGTGTACA ATGTTCTTAG 
     3661 TGAACTGGAG AAGACATTTA ACCTGCCAGT TCTGGAAGCA CTGTTCAGCG ATGTCAACAT 
     3721 GCAGGAATAC CCCGATTTAA TTCACATTTA TAAAGGCTTT GAAAATGTAA TCCATGACAA 
     3781 ATTGCCTCTC CAAGAAAGTG AAGAAGAAGA GAGGGAGGAG AGGTCTGGCC TCCAACTAAG 
     3841 TCTTGAACAA GGAACTGGTG AAAACTCTTT TCGAAGCCTG ACTTGGCCAC CTTCGGGTTC 
     3901 CCCATCTCAT GCTGGTACAA CCCCACCTGA AAATGGACTC TCAGAGCACC CCTGTGAAAC 
     3961 AGAACAGATA AATGCAAAGA GAAAAGATAC AACCAGTGAC AAAGATGATT CGCTAGGAAG 
     4021 CCAACAAACA AATGAACAAT GTGCTCAAAA GGCTGAGCCA ACAGAGTCCT GCGAACAAAT 
     4081 TGCTGTCCAA GTGAATAATG GGGATGCTGG AAGGGAGATG CCCTGCCCGT TGCCCTGTGA 
     4141 TGAAGAAAGC CCAGAGGCAG AGCTACACAA CCATGGAATC CAAATTAATT CCTGTTCTGT 
     4201 GCGACTGGTG GATATAAAAA AGGAAAAGCC ATTTTCTAAT TCAAAAGTTG AGTGCCAAGC 
     4261 CCAAGCAAGA ACTCATCATA ACCAGGCATC TGACATAATA GTCATCAGCA GTGAGGACTC 
     4321 TGAAGGATCC ACTGACGTTG ATGAGCCCTT AGAAGTCTTC ATCTCAGCAC CGAGAAGTGA 
     4381 GCCTGTGATC AATAATGACA ACCCTTTAGA ATCAAATGAT GAAAAGGAGG GCCAAGAAGC 
     4441 CACTTGCTCA CGACCCCAGA TTGTACCAGA GCCCATGGAT TTCAGAAAAT TATCTACATT 
     4501 CAGAGAAAGT TTTAAGAAAA GAGTGATAGG ACAAGACCAC GACTTTTCAG AATCCAGTGA 
     4561 GGAGGAGGCG CCCGCAGAAG CCTCGAGCGG GGCACTGAGA AGCAAGCATG GTGAGAAGGC 
     4621 TCCTATGACT TCTAGAAGTA CATCTACTTG GAGAATACCC AGCAGGAAGA GACGTTTCAG 
     4681 CAGTAGTGAC TTTTCAGACC TGAGTAATGG AGAAGAGCTT CAGGAAACCT GCAGCTCATC 
     4741 CCTAAGAAGA GGGTCAGGAT CACAGCCACA AGAACCTGAA AATAAGAAGT GCTCCTGTGT 
     4801 CATGTGTTTT CCAAAAGGTG TGCCAAGAAG CCAAGAAGCA AGGACTGAAA GTAGTCAAGC 
     4861 ATCTGACATG ATGGATACCA TGGATGTTGA AAACAATTCT ACTTTGGAAA AACACAGTGG 
     4921 GAAAAGAAGA AAAAAGAGAA GGCATAGATC TAAAGTAAAT GGTCTCCAAA GAGGGAGAAA 
     4981 GAAAGACAGA CCTAGAAAAC ATTTAACTCT GAATAACAAA GTCCAAAAGA AAAGATGGCA 
     5041 ACAAAGAGGA AGAAAAGCCA ACACTAGACC TTTGAAAAGA AGAAGAAAAA GAGGTCCAAG 
     5101 AATTCCCAAA GATGAAAATA TTAATTTTAA ACAATCTGAA CTTCCTGTGA CCTGTGGTGA 
     5161 GGTGAAGGGC ACTCTATATA AGGAGCGATT CAAACAAGGA ACCTCAAAGA AGTGTATACA 
     5221 GAGTGAGGAT AAAAAGTGGT TCACTCCCAG GGAATTTGAA ATTGAAGGAG ACCGCGGAGC 
     5281 ATCCAAGAAC TGGAAGCTAA GTATACGCTG CGGTGGATAT ACCCTGAAAG TCCTGATGGA 
     5341 GAACAAATTT CTGCCAGAAC CACCAAGCAC AAGAAAAAAG AGAATACTGG AATCTCACAA 
     5401 CAATACCTTA GTTGACCCTT GTCCGGAAAA CTCAAATATA TGTGAGGTGT GCAACAAATG 
     5461 GGGACGGCTG TTCTGCTGCG ACACTTGTCC AAGATCCTTT CATGAGCACT GCCACATCCC 
     5521 ATCCGTGGAA GCTAACAAGA ACCCGTGGAG TTGCATCTTC TGCAGGATAA AGACTATTCA 
     5581 GGAAAGATGC CCAGAAAGCC AATCAGGTCA TCAGGAATCT GAAGTCCTGA TGAGGCAGAT 
     5641 GCTGCCTGAG GAGCAGTTGA AATGTGAATT CCTCCTCTTG AAGGTCTACT GTGATTCGAA 
     5701 AAGCTGCTTT TTCGCCTCAG AACCGTATTA TAACAGAGAG GGGTCTCAGG GCCCACAGAA 
     5761 GCCCATGTGG TTAAACAAAG TCAAGACAAG TTTGAATGAG CAGATGTACA CCCGAGTAGA 
     5821 AGGGTTTGTG CAGGACATGC GTCTCATCTT TCATAACCAC AAGGAATTTT ACAGGGAAGA 
     5881 TAAATTCACC AGACTGGGAA TTCAAGTACA GGACATCTTT GAGAAGAATT TCAGAAACAT 
     5941 TTTTGCAATT CAGGAAACAA GCAAGAACAT TATAATGTTT ATTACGCGTA CGCGGCCGCT 
     6001 CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA 
     6061 TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCTGTAC AAGTAGGATT CGTCGAGGGA 
     6121 CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAC ATGTTTAAGG GTTCCGGTTC 
     6181 CACTAGGTAC AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT 
     6241 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 
     6301 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 
     6361 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 
     6421 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 
     6481 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 
     6541 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 
     6601 TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 
     6661 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 
     6721 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 
     6781 TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCTC GATCGAGACC TAGAAAAACA 
     6841 TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC 
     6901 ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC 
     6961 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT 
     7021 AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA 
     7081 CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC AGATATCCAC TGACCTTTGG 
     7141 ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA 
     7201 GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT 
     7261 ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC ATGGCCCGAG AGCTGCATCC 
     7321 GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 
     7381 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 
     7441 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 
     7501 AATCTCTAGC AGCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     7561 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     7621 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     7681 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     7741 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     7801 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     7861 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     7921 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     7981 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     8041 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     8101 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     8161 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     8221 AGGGATTTTG GTCATGATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA 
     8281 TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 
     8341 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG 
     8401 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT 
     8461 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA 
     8521 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC 
     8581 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 
     8641 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC 
     8701 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC 
     8761 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC 
     8821 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA 
     8881 GTGATTTTTT TCTCCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8941 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTCCCG 
     9001 CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//