LOCUS       RC212265L1                9531 bp    DNA     circular  SYN 
DEFINITION  MBTPS1 (Myc-DDK-tagged)-Human membrane-bound transcription factor peptidase, site 1 (MBTPS1)
ACCESSION   NM_003791
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7822..8002 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8730..9389 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8161..8729 
                     /label="ColE1"  
     misc_feature    6697..6717 
                     /label="LR50 primer"  
     misc_feature    6494..6565 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6706..7297 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6476..6482 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6476 
                     /label="MBTPS1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAGCTTGTC AACATCTGGC TGCTTCTGCT 
     3361 CGTGGTTTTG CTCTGTGGGA AGAAACATCT GGGCGACAGA CTGGAAAAGA AATCTTTTGA 
     3421 AAAGGCCCCA TGCCCTGGCT GTTCCCACCT GACTTTGAAG GTGGAATTCT CATCAACAGT 
     3481 TGTGGAATAT GAATATATTG TGGCTTTCAA TGGATACTTT ACAGCCAAAG CTAGAAATTC 
     3541 ATTTATTTCA AGTGCCCTGA AGAGCAGTGA AGTAGACAAT TGGAGAATTA TACCTCGAAA 
     3601 CAATCCATCC AGTGACTACC CTAGTGATTT TGAGGTGATT CAGATAAAAG AAAAACAGAA 
     3661 AGCGGGGCTG CTAACACTTG AAGATCATCC AAACATCAAA CGGGTCACGC CCCAACGAAA 
     3721 AGTCTTTCGT TCCCTCAAGT ATGCTGAATC TGACCCCACA GTACCCTGCA ATGAAACCCG 
     3781 GTGGAGCCAG AAGTGGCAAT CATCACGTCC CCTGCGAAGA GCCAGCCTCT CCCTGGGCTC 
     3841 TGGCTTCTGG CATGCTACGG GAAGGCATTC GAGCAGACGG CTGCTGAGAG CCATCCCGCG 
     3901 CCAGGTTGCC CAGACACTGC AGGCAGATGT GCTCTGGCAG ATGGGATATA CAGGTGCTAA 
     3961 TGTAAGAGTT GCTGTTTTTG ACACTGGGCT GAGCGAGAAG CATCCCCACT TCAAAAATGT 
     4021 GAAGGAGAGA ACCAACTGGA CCAACGAGCG AACGCTGGAC GATGGGTTGG GCCATGGCAC 
     4081 ATTCGTGGCA GGTGTGATAG CCAGCATGAG GGAGTGCCAA GGATTTGCTC CAGATGCAGA 
     4141 ACTTCACATT TTCAGGGTCT TTACCAATAA TCAGGTATCT TACACATCTT GGTTTTTGGA 
     4201 CGCCTTCAAC TATGCCATTT TAAAGAAGAT CGACGTGTTA AACCTCAGCA TCGGCGGCCC 
     4261 GGACTTCATG GATCATCCGT TTGTTGACAA GGTGTGGGAA TTAACAGCTA ACAATGTAAT 
     4321 CATGGTTTCT GCTATTGGCA ATGACGGACC TCTTTATGGC ACTCTGAATA ACCCTGCTGA 
     4381 TCAAATGGAT GTGATTGGAG TAGGCGGCAT TGACTTTGAA GATAACATCG CCCGCTTTTC 
     4441 TTCAAGGGGA ATGACTACCT GGGAGCTACC AGGAGGCTAC GGTCGCATGA AACCTGACAT 
     4501 TGTCACCTAT GGTGCTGGCG TGCGGGGTTC TGGCGTGAAA GGGGGGTGCC GGGCCCTCTC 
     4561 AGGGACCAGT GTTGCTTCTC CAGTGGTTGC AGGTGCTGTC ACCTTGTTAG TGAGCACAGT 
     4621 CCAGAAGCGT GAGCTGGTGA ATCCCGCCAG TATGAAGCAG GCCCTGATCG CGTCAGCCCG 
     4681 GAGGCTCCCC GGGGTCAACA TGTTTGAGCA AGGCCACGGC AAGCTCGATC TGCTCAGAGC 
     4741 CTATCAGATC CTCAACAGCT ACAAGCCACA GGCAAGTTTG AGCCCCAGCT ACATAGATCT 
     4801 GACTGAGTGT CCCTACATGT GGCCCTACTG CTCCCAGCCC ATCTACTATG GAGGAATGCC 
     4861 GACAGTTGTT AATGTCACCA TCCTCAACGG CATGGGAGTC ACAGGAAGAA TTGTAGATAA 
     4921 GCCTGACTGG CAGCCCTATT TGCCACAGAA CGGAGACAAC ATTGAAGTTG CCTTCTCCTA 
     4981 CTCCTCGGTC TTATGGCCTT GGTCGGGCTA CCTGGCCATC TCCATTTCTG TGACCAAGAA 
     5041 AGCGGCTTCC TGGGAAGGCA TTGCTCAGGG CCATGTCATG ATCACTGTGG CTTCCCCAGC 
     5101 AGAGACAGAG TCAAAAAATG GTGCAGAACA GACTTCAACA GTAAAGCTCC CCATTAAGGT 
     5161 GAAGATAATT CCTACTCCCC CGCGAAGCAA GAGAGTTCTC TGGGATCAGT ACCACAACCT 
     5221 CCGCTATCCA CCTGGCTATT TCCCCAGGGA TAATTTAAGG ATGAAGAATG ACCCTTTAGA 
     5281 CTGGAATGGT GATCACATCC ACACCAATTT CAGGGATATG TACCAGCATC TGAGAAGCAT 
     5341 GGGCTACTTT GTAGAGGTCC TCGGGGCCCC CTTCACGTGT TTTGATGCCA GTCAGTATGG 
     5401 CACTTTGCTG ATGGTGGACA GTGAGGAGGA GTACTTCCCT GAAGAGATCG CCAAGCTCCG 
     5461 GAGGGACGTG GACAACGGCC TCTCGCTCGT CATCTTCAGT GACTGGTACA ACACTTCTGT 
     5521 TATGAGAAAA GTGAAGTTTT ATGATGAAAA CACAAGGCAG TGGTGGATGC CGGATACCGG 
     5581 AGGAGCTAAC ATCCCAGCTC TGAATGAGCT GCTGTCTGTG TGGAACATGG GGTTCAGCGA 
     5641 TGGCCTGTAT GAAGGGGAGT TCACCCTGGC CAACCATGAC ATGTATTATG CGTCAGGGTG 
     5701 CAGCATCGCG AAGTTTCCAG AAGATGGCGT CGTGATAACA CAGACTTTCA AGGACCAAGG 
     5761 ATTGGAGGTT TTAAAGCAGG AAACAGCAGT TGTTGAAAAC GTCCCCATTT TGGGACTTTA 
     5821 TCAGATTCCA GCTGAGGGTG GAGGCCGGAT TGTACTGTAT GGGGACTCCA ATTGCTTGGA 
     5881 TGACAGTCAC CGACAGAAGG ACTGCTTTTG GCTTCTGGAT GCCCTCCTCC AGTACACATC 
     5941 GTATGGGGTG ACACCGCCTA GCCTCAGTCA CTCTGGGAAC CGCCAGCGCC CTCCCAGTGG 
     6001 AGCAGGCTCA GTCACTCCAG AGAGGATGGA AGGAAACCAT CTTCATCGGT ACTCCAAGGT 
     6061 TCTGGAGGCC CATTTGGGAG ACCCAAAACC TCGGCCTCTA CCAGCCTGCC CACGCTTGTC 
     6121 TTGGGCCAAG CCACAGCCTT TAAACGAGAC GGCGCCCAGT AACCTTTGGA AACATCAGAA 
     6181 GCTACTCTCC ATTGACCTGG ACAAGGTGGT GTTACCCAAC TTTCGATCGA ATCGCCCTCA 
     6241 AGTGAGGCCC TTGTCCCCTG GAGAGAGCGG CGCCTGGGAC ATTCCTGGAG GGATCATGCC 
     6301 TGGCCGCTAC AACCAGGAGG TGGGCCAGAC CATTCCTGTC TTTGCCTTCC TGGGAGCCAT 
     6361 GGTGGTCCTG GCCTTCTTTG TGGTACAAAT CAACAAGGCC AAGAGCAGGC CGAAGCGGAG 
     6421 GAAGCCCAGG GTGAAGCGCC CGCAGCTCAT GCAGCAGGTT CACCCGCCAA AGACCCCTTC 
     6481 GGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     6541 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6601 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6661 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6721 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6781 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6841 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6901 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6961 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     7021 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     7081 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     7141 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     7201 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     7261 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     7321 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     7381 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     7441 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     7501 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     7561 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7621 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7681 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7741 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7801 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7861 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7921 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7981 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     8041 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     8101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     8161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     8221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     8281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     8341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     8401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     8461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     8521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     8581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8641 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8701 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8761 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8821 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8881 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8941 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     9001 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     9061 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     9121 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     9181 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     9241 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     9301 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     9361 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     9421 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     9481 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//