LOCUS       RC212265                8037 bp    DNA     circular  SYN 
DEFINITION  MBTPS1 (Myc-DDK-tagged)-Human membrane-bound transcription factor peptidase, site 1 (MBTPS1)
ACCESSION   NM_003791
VERSION     NM_003791.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4194..4285 
                     /label="Myc-DDK"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    4372..4391 
                     /label="Restriction Enzyme"  
     misc_feature    5176..5848 
                     /label="ColE1"  
     misc_feature    6278..7072 
                     /label="Kan/Neo"  
     misc_feature    7107..7409 
                     /label="SV40 ori"  
     misc_feature    7495..7972 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4176..4182 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4176 
                     /label="MBTPS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTTGTC AACATCTGGC TGCTTCTGCT CGTGGTTTTG CTCTGTGGGA 
     1081 AGAAACATCT GGGCGACAGA CTGGAAAAGA AATCTTTTGA AAAGGCCCCA TGCCCTGGCT 
     1141 GTTCCCACCT GACTTTGAAG GTGGAATTCT CATCAACAGT TGTGGAATAT GAATATATTG 
     1201 TGGCTTTCAA TGGATACTTT ACAGCCAAAG CTAGAAATTC ATTTATTTCA AGTGCCCTGA 
     1261 AGAGCAGTGA AGTAGACAAT TGGAGAATTA TACCTCGAAA CAATCCATCC AGTGACTACC 
     1321 CTAGTGATTT TGAGGTGATT CAGATAAAAG AAAAACAGAA AGCGGGGCTG CTAACACTTG 
     1381 AAGATCATCC AAACATCAAA CGGGTCACGC CCCAACGAAA AGTCTTTCGT TCCCTCAAGT 
     1441 ATGCTGAATC TGACCCCACA GTACCCTGCA ATGAAACCCG GTGGAGCCAG AAGTGGCAAT 
     1501 CATCACGTCC CCTGCGAAGA GCCAGCCTCT CCCTGGGCTC TGGCTTCTGG CATGCTACGG 
     1561 GAAGGCATTC GAGCAGACGG CTGCTGAGAG CCATCCCGCG CCAGGTTGCC CAGACACTGC 
     1621 AGGCAGATGT GCTCTGGCAG ATGGGATATA CAGGTGCTAA TGTAAGAGTT GCTGTTTTTG 
     1681 ACACTGGGCT GAGCGAGAAG CATCCCCACT TCAAAAATGT GAAGGAGAGA ACCAACTGGA 
     1741 CCAACGAGCG AACGCTGGAC GATGGGTTGG GCCATGGCAC ATTCGTGGCA GGTGTGATAG 
     1801 CCAGCATGAG GGAGTGCCAA GGATTTGCTC CAGATGCAGA ACTTCACATT TTCAGGGTCT 
     1861 TTACCAATAA TCAGGTATCT TACACATCTT GGTTTTTGGA CGCCTTCAAC TATGCCATTT 
     1921 TAAAGAAGAT CGACGTGTTA AACCTCAGCA TCGGCGGCCC GGACTTCATG GATCATCCGT 
     1981 TTGTTGACAA GGTGTGGGAA TTAACAGCTA ACAATGTAAT CATGGTTTCT GCTATTGGCA 
     2041 ATGACGGACC TCTTTATGGC ACTCTGAATA ACCCTGCTGA TCAAATGGAT GTGATTGGAG 
     2101 TAGGCGGCAT TGACTTTGAA GATAACATCG CCCGCTTTTC TTCAAGGGGA ATGACTACCT 
     2161 GGGAGCTACC AGGAGGCTAC GGTCGCATGA AACCTGACAT TGTCACCTAT GGTGCTGGCG 
     2221 TGCGGGGTTC TGGCGTGAAA GGGGGGTGCC GGGCCCTCTC AGGGACCAGT GTTGCTTCTC 
     2281 CAGTGGTTGC AGGTGCTGTC ACCTTGTTAG TGAGCACAGT CCAGAAGCGT GAGCTGGTGA 
     2341 ATCCCGCCAG TATGAAGCAG GCCCTGATCG CGTCAGCCCG GAGGCTCCCC GGGGTCAACA 
     2401 TGTTTGAGCA AGGCCACGGC AAGCTCGATC TGCTCAGAGC CTATCAGATC CTCAACAGCT 
     2461 ACAAGCCACA GGCAAGTTTG AGCCCCAGCT ACATAGATCT GACTGAGTGT CCCTACATGT 
     2521 GGCCCTACTG CTCCCAGCCC ATCTACTATG GAGGAATGCC GACAGTTGTT AATGTCACCA 
     2581 TCCTCAACGG CATGGGAGTC ACAGGAAGAA TTGTAGATAA GCCTGACTGG CAGCCCTATT 
     2641 TGCCACAGAA CGGAGACAAC ATTGAAGTTG CCTTCTCCTA CTCCTCGGTC TTATGGCCTT 
     2701 GGTCGGGCTA CCTGGCCATC TCCATTTCTG TGACCAAGAA AGCGGCTTCC TGGGAAGGCA 
     2761 TTGCTCAGGG CCATGTCATG ATCACTGTGG CTTCCCCAGC AGAGACAGAG TCAAAAAATG 
     2821 GTGCAGAACA GACTTCAACA GTAAAGCTCC CCATTAAGGT GAAGATAATT CCTACTCCCC 
     2881 CGCGAAGCAA GAGAGTTCTC TGGGATCAGT ACCACAACCT CCGCTATCCA CCTGGCTATT 
     2941 TCCCCAGGGA TAATTTAAGG ATGAAGAATG ACCCTTTAGA CTGGAATGGT GATCACATCC 
     3001 ACACCAATTT CAGGGATATG TACCAGCATC TGAGAAGCAT GGGCTACTTT GTAGAGGTCC 
     3061 TCGGGGCCCC CTTCACGTGT TTTGATGCCA GTCAGTATGG CACTTTGCTG ATGGTGGACA 
     3121 GTGAGGAGGA GTACTTCCCT GAAGAGATCG CCAAGCTCCG GAGGGACGTG GACAACGGCC 
     3181 TCTCGCTCGT CATCTTCAGT GACTGGTACA ACACTTCTGT TATGAGAAAA GTGAAGTTTT 
     3241 ATGATGAAAA CACAAGGCAG TGGTGGATGC CGGATACCGG AGGAGCTAAC ATCCCAGCTC 
     3301 TGAATGAGCT GCTGTCTGTG TGGAACATGG GGTTCAGCGA TGGCCTGTAT GAAGGGGAGT 
     3361 TCACCCTGGC CAACCATGAC ATGTATTATG CGTCAGGGTG CAGCATCGCG AAGTTTCCAG 
     3421 AAGATGGCGT CGTGATAACA CAGACTTTCA AGGACCAAGG ATTGGAGGTT TTAAAGCAGG 
     3481 AAACAGCAGT TGTTGAAAAC GTCCCCATTT TGGGACTTTA TCAGATTCCA GCTGAGGGTG 
     3541 GAGGCCGGAT TGTACTGTAT GGGGACTCCA ATTGCTTGGA TGACAGTCAC CGACAGAAGG 
     3601 ACTGCTTTTG GCTTCTGGAT GCCCTCCTCC AGTACACATC GTATGGGGTG ACACCGCCTA 
     3661 GCCTCAGTCA CTCTGGGAAC CGCCAGCGCC CTCCCAGTGG AGCAGGCTCA GTCACTCCAG 
     3721 AGAGGATGGA AGGAAACCAT CTTCATCGGT ACTCCAAGGT TCTGGAGGCC CATTTGGGAG 
     3781 ACCCAAAACC TCGGCCTCTA CCAGCCTGCC CACGCTTGTC TTGGGCCAAG CCACAGCCTT 
     3841 TAAACGAGAC GGCGCCCAGT AACCTTTGGA AACATCAGAA GCTACTCTCC ATTGACCTGG 
     3901 ACAAGGTGGT GTTACCCAAC TTTCGATCGA ATCGCCCTCA AGTGAGGCCC TTGTCCCCTG 
     3961 GAGAGAGCGG CGCCTGGGAC ATTCCTGGAG GGATCATGCC TGGCCGCTAC AACCAGGAGG 
     4021 TGGGCCAGAC CATTCCTGTC TTTGCCTTCC TGGGAGCCAT GGTGGTCCTG GCCTTCTTTG 
     4081 TGGTACAAAT CAACAAGGCC AAGAGCAGGC CGAAGCGGAG GAAGCCCAGG GTGAAGCGCC 
     4141 CGCAGCTCAT GCAGCAGGTT CACCCGCCAA AGACCCCTTC GGTGACGCGT ACGCGGCCGC 
     4201 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4261 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4321 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4381 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4441 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4501 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4561 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4621 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4681 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4741 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4801 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4861 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4921 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5521 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5761 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5821 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5881 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5941 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6001 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6061 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6121 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6181 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6241 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6301 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6361 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6421 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6481 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6541 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6601 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6661 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6721 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6781 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6841 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6901 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6961 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7021 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7081 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7141 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7201 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7261 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7441 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7501 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7561 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7621 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7681 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7741 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7801 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7861 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7921 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7981 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//