LOCUS       RC212262                9066 bp    DNA     circular  SYN 
DEFINITION  GPRASP1 (Myc-DDK-tagged)-Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 3
ACCESSION   NM_001099411
VERSION     NM_001099411.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5223..5314 
                     /label="Myc-DDK"  
     misc_feature    5344..5929 
                     /label="PolyA signal"  
     misc_feature    5401..5420 
                     /label="Restriction Enzyme"  
     misc_feature    6205..6877 
                     /label="ColE1"  
     misc_feature    7307..8101 
                     /label="Kan/Neo"  
     misc_feature    8136..8438 
                     /label="SV40 ori"  
     misc_feature    8524..9001 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5205..5211 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5205 
                     /label="GPRASP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGGGGCA GAGATTGAGT CTGGTGCCCA GGTCAAGCCT GAAAAGAAGC 
     1081 CTGGGGAAGA GGTTGTAGGT GGGGCTGAGA TAGAGAATGA TGTCCCTCTG GTGGTCAGAC 
     1141 CCAAGGTTAG GACCCAGGCC CAGATAATGC CTGGGGCAAG GCCCAAGAAT AAGTCCAAGG 
     1201 TTATGCCTGG AGCAAGCACC AAAGTTGAGA CAAGTGCAGT GGGTGGGGCA CGCCCTAAGA 
     1261 GTAAGGCCAA GGCAATACCT GTTTCACGAT TTAAGGAAGA AGCCCAGATG TGGGCTCAGC 
     1321 CCAGGTTTGG TGCTGAAAGA TTGTCTAAGA CAGAGAGAAA CTCCCAGACC AATATCATAG 
     1381 CCTCTCCACT TGTCAGTACT GATTCTGTCT TGGTTGCTAA AACAAAGTAC CTGTCTGAGG 
     1441 ATAGAGAACT GGTTAATACA GACACTGAGA GCTTTCCTAG AAGGAAGGCC CATTACCAAG 
     1501 CAGGATTCCA GCCTTCTTTT AGGTCAAAGG AGGAGACCAA TATGGGGTCC TGGTGCTGTC 
     1561 CTAGGCCTAC ATCCAAACAA GAAGCCTCTC CTAATTCTGA TTTCAAATGG GTAGACAAAT 
     1621 CTGTGAGTTC CTTGTTCTGG AGTGGAGATG AGGTCACTGC AAAATTTCAT CCTGGGAATA 
     1681 GGGTAAAAGA CAGTAACAGA TCCATGCACA TGGCCAATCA AGAGGCTAAT ACCATGTCTA 
     1741 GGTCCCAAAC TAACCAGGAG CTCTATATTG CATCTAGTTC TGGTTCTGAG GATGAGTCTG 
     1801 TTAAGACACC CTGGTTCTGG GCCAGAGATA AAACCAATAC CTGGTCTGGG CCCAGGGAAG 
     1861 ATCCCAATAG CAGGTCCAGG TTTAGGTCTA AGAAAGAAGT CTATGTTGAA TCAAGTTCTG 
     1921 GATCTGAGCA TGAAGACCAT TTGGAGTCCT GGTTTGGGGC TGGAAAGGAG GCCAAATTCA 
     1981 GGTCCAAAAT GAGAGCTGGG AAGGAGGCCA ATAACAGGGC CAGGCACAGG GCCAAGCGAG 
     2041 AAGCTTGCAT TGATTTCATG CCTGGGTCTA TAGATGTAAT TAAAAAAGAG TCCTGTTTCT 
     2101 GGCCTGAAGA AAATGCTAAT ACCTTTTCAA GGCCCATGAT CAAGAAAGAG GCCAGGGCCA 
     2161 GAGCAATGAC AAAGGAAGAG GCCAAAACCA AGGCCCGAGC CAGGGCCAAG CAAGAAGCCA 
     2221 GGTCAGAGGA GGAAGCCCTC ATTGGGACCT GGTTCTGGGC TACAGACGAG TCCAGCATGG 
     2281 CAGATGAAGC CAGCATAGAG TCCAGTCTAC AAGTGGAGGA TGAGTCCATA ATTGGGAGTT 
     2341 GGTTCTGGAC TGAAGAAGAG GCCAGTATGG GGACTGGGGC TAGCAGTAAA TCCAGACCAA 
     2401 GGACTGATGG GGAGCGTATT GGTGATTCCT TATTTGGGGC TAGGGAAAAG ACCAGTATGA 
     2461 AAACTGGGGC TGAGGCCACC TCTGAATCTA TACTAGCAGC TGATGATGAA CAGGTCATTA 
     2521 TTGGTTCCTG GTTCTGGGCT GGTGAAGAGG TCAACCAAGA GGCTGAGGAA GAGACCATTT 
     2581 TTGGGTCGTG GTTCTGGGTC ATTGATGCGG CCAGTGTGGA ATCTGGTGTT GGGGTCAGCT 
     2641 GTGAGTCCAG GACAAGGTCT GAGGAAGAAG AGGTCATTGG TCCCTGGTTT TGGTCTGGAG 
     2701 AACAAGTTGA TATAGAGGCT GGAATCGGAG AAGAGGCCAG GCCAGGAGCT GAAGAAGAGA 
     2761 CAATATTCGG GTCCTGGTTT TGGGCTGAAA ACCAGACCTA TATGGATTGT AGGGCTGAAA 
     2821 CTAGCTGTGA CACCATGCAA GGGGCTGAGG AGGAGGAGCC CATTATTGGG TCCTGGTTTT 
     2881 GGACCAGAGT AGAAGCTTGT GTGGAGGGTG ATGTCAACAG CAAGTCTAGC CTGGAGGACA 
     2941 AGGAAGAGGC CATGATACCA TGTTTTGGAG CCAAAGAAGA GGTCAGTATG AAGCATGGGA 
     3001 CTGGTGTCAG ATGCAGATTT ATGGCAGGGG CTGAGGAGAC CAATAATAAG TCTTGCTTCT 
     3061 GGGCAGAAAA AGAACCCTGT ATGTATCCTG CCGGTGGAGG AAGTTGGAAG TCTAGGCCAG 
     3121 AGGAGGAAGA GGACATTGTC AATTCGTGGT TCTGGTCCAG AAAATACACA AAGCCAGAGG 
     3181 CCATTATAGG GTCCTGGTTA TGGGCTACAG AAGAGAGTAA TATAGATGGG ACTGGAGAAA 
     3241 AGGCCAAGTT ACTGACTGAA GAGGAGACCA TAATCAATTC CTGGTTCTGG AAAGAAGATG 
     3301 AAGCCATTTC AGAGGCTACT GACAGAGAAG AGTCCAGGCC AGAAGCTGAG GAGGGGGACA 
     3361 TTATTGGTTC TTGGTTCTGG GCTGGAGAAG AGGACAGACT AGAGCCAGCT GCTGAGACTA 
     3421 GAGAAGAAGA CAGGCTAGCA GCTGAGAAAG AAGGTATTGT TGGGTCCTGG TTTGGGGCCA 
     3481 GAGAAGAGAC CATTAGAAGA GAGGCTGGGT CTTGCAGCAA ATCCAGTCCT AAAGCTGAAG 
     3541 AGGAAGAAGT CATTATTGGG TCCTGGTTCT GGGAAGAAGA GGCCAGTCCG GAGGCAGTGG 
     3601 CAGGAGTCGG CTTTGAGTCA AAGCCTGGGA CTGAGGAGGA AGAAATCACT GTTGGGTCCT 
     3661 GGTTCTGGCC TGAAGAAGAA GCCAGTATAC AGGCTGGATC TCAGGCAGTA GAGGAAATGG 
     3721 AGTCAGAGAC TGAAGAGGAA ACCATTTTTG GGTCCTGGTT CTGGGATGGA AAAGAAGTCA 
     3781 GTGAAGAAGC AGGACCATGC TGTGTATCCA AGCCAGAGGA TGATGAAGAG ATGATTGTTG 
     3841 AGTCCTGGTT CTGGTCTAGA GACAAAGCCA TTAAGGAAAC TGGAACTGTG GCCACCTGTG 
     3901 AGTCCAAGCC AGAAAATGAG GAAGGGGCCA TTGTTGGGTC TTGGTTTGAG GCTGAAGATG 
     3961 AGGTAGATAA CAGGACTGAC AATGGAAGCA ACTGTGGGTC CAGGACATTA GCTGATGAAG 
     4021 ATGAGGCCAT AGTGGGGTCC TGGTTCTGGG CAGGAGATGA GGCCCATTTT GAATCAAATC 
     4081 CTAGCCCCGT GTTCAGGGCC ATTTGCAGGT CCACGTGTTC AGTTGAACAG GAGCCTGATC 
     4141 CTTCACGCAG GCCTCAGAGT TGGGAGGAGG TCACTGTTCA GTTCAAGCCT GGTCCATGGG 
     4201 GTAGGGTCGG CTTCCCATCT ATAAGCCCCT TTAGATTTCC GAAAGAGGCA GCATCTTTAT 
     4261 TCTGTGAAAT GTTTGGGGGC AAACCCAGGA ACATGGTACT TAGCCCAGAA GGGGAAGATC 
     4321 AGGAATCTTT GCTTCAGCCT GATCAGCCTA GTCCTGAGTT CCCATTTCAG TATGATCCTT 
     4381 CCTACAGGTC AGTCCAGGAA ATTCGAGAGC ATCTTAGGGC CAAGGAGAGT ACAGAGCCTG 
     4441 AGAGTTCATC CTGTAACTGC ATACAATGTG AGCTGAAAAT TGGTTCTGAA GAGTTTGAAG 
     4501 AACTCCTTTT ATTAATGGAA AAAATTCGGG ATCCTTTTAT TCATGAAATA TCTAAAATCG 
     4561 CAATGGGTAT GAGAAGTGCT TCTCAATTTA CCCGAGATTT CATTCGAGAT TCAGGTGTTG 
     4621 TCTCACTTAT TGAAACCTTG CTTAATTATC CGTCCTCCCG AGTTAGAACA AGTTTTTTGG 
     4681 AAAATATGAT TCGCATGGCC CCACCTTATC CGAATCTAAA CATAATTCAG ACATACATAT 
     4741 GTAAAGTGTG TGAGGAAACC CTTGCTTATA GCGTGGATTC CCCGGAACAG CTGTCTGGAA 
     4801 TAAGGATGAT TAGACATCTC ACTACTACTA CTGACTATCA CACACTGGTT GCCAATTATA 
     4861 TGTCTGGGTT TCTCTCCTTA TTAGCTACAG GCAATGCCAA AACAAGGTTT CATGTTTTGA 
     4921 AAATGCTACT GAATTTGTCT GAAAATCTTT TCATGACAAA AGAACTACTC AGTGCTGAAG 
     4981 CAGTGTCAGA ATTTATAGGC CTCTTTAACA GGGAAGAGAC AAATGACAAT ATTCAAATTG 
     5041 TTCTTGCAAT ATTTGAGAAT ATTGGCAACA ATATCAAAAA AGAAACAGTG TTCTCTGATG 
     5101 ATGATTTCAA TATTGAGCCG CTTATTTCTG CATTCCACAA AGTTGAGAAA TTTGCTAAGG 
     5161 AACTGCAAGG CAAAACAGAC AATCAAAATG ACCCTGAAGG GGACCAAGAA AATACGCGTA 
     5221 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5281 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5341 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5401 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5461 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5521 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5581 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5641 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5701 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5761 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5821 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5881 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5941 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6001 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6061 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6121 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6181 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6241 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6301 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6361 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6421 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6481 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6541 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6601 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6661 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6721 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6781 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6841 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6901 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6961 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7021 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7081 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7141 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7201 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7261 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7321 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7381 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7441 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7501 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7561 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7621 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7681 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7741 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7801 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7861 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7921 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7981 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8041 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8101 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8161 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8221 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8281 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8341 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8401 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8461 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8521 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8581 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8641 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8701 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8761 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8821 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8881 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8941 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9001 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9061 AATTTT
//