LOCUS       RC212235                9273 bp    DNA     circular  SYN 
DEFINITION  NCOA2 (Myc-DDK-tagged)-Human nuclear receptor coactivator 2 (NCOA2)
ACCESSION   NM_006540
VERSION     NM_006540.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5430..5521 
                     /label="Myc-DDK"  
     misc_feature    5551..6136 
                     /label="PolyA signal"  
     misc_feature    5608..5627 
                     /label="Restriction Enzyme"  
     misc_feature    6412..7084 
                     /label="ColE1"  
     misc_feature    7514..8308 
                     /label="Kan/Neo"  
     misc_feature    8343..8645 
                     /label="SV40 ori"  
     misc_feature    8731..9208 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5418 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="NCOA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGGATG GGAGAAAATA CCTCTGACCC CTCCAGGGCA GAGACAAGAA 
     1081 AGCGCAAGGA ATGTCCTGAC CAACTTGGAC CCAGCCCCAA AAGGAACACT GAAAAACGTA 
     1141 ATCGTGAACA GGAAAATAAA TATATAGAAG AACTTGCAGA GTTGATTTTT GCAAATTTTA 
     1201 ATGATATAGA CAACTTTAAC TTCAAACCTG ACAAATGTGC AATCTTAAAA GAAACTGTGA 
     1261 AGCAAATTCG TCAGATCAAA GAACAAGAGA AAGCAGCAGC TGCCAACATA GATGAAGTGC 
     1321 AGAAGTCAGA TGTATCCTCT ACAGGGCAGG GTGTCATCGA CAAGGATGCG CTGGGGCCTA 
     1381 TGATGCTTGA GGCCCTTGAT GGGTTCTTCT TTGTAGTGAA CCTGGAAGGC AACGTTGTGT 
     1441 TTGTGTCAGA GAATGTGACA CAGTATCTAA GGTATAACCA AGAAGAGCTG ATGAACAAAA 
     1501 GTGTATATAG CATCTTGCAT GTTGGGGACC ACACGGAATT TGTCAAAAAC CTGCTGCCAA 
     1561 AGTCTATAGT AAATGGGGGA TCTTGGTCTG GCGAACCTCC GAGGCGGAAC AGCCATACCT 
     1621 TCAATTGTCG GATGCTGGTA AAACCTTTAC CTGATTCAGA AGAGGAGGGT CATGATAACC 
     1681 AGGAAGCTCA TCAGAAATAT GAAACTATGC AGTGCTTCGC TGTCTCTCAA CCAAAGTCCA 
     1741 TCAAAGAAGA AGGAGAAGAT TTGCAGTCCT GCTTGATTTG CGTGGCAAGA AGAGTTCCCA 
     1801 TGAAGGAAAG ACCAGTTCTT CCCTCATCAG AAAGTTTTAC TACTCGCCAG GATCTCCAAG 
     1861 GCAAGATCAC GTCTCTGGAT ACCAGCACCA TGAGAGCAGC CATGAAACCA GGCTGGGAGG 
     1921 ACCTGGTAAG AAGGTGTATT CAGAAGTTCC ATGCGCAGCA TGAAGGAGAA TCTGTGTCCT 
     1981 ATGCTAAGAG GCATCATCAT GAAGTACTGA GACAAGGATT GGCATTCAGT CAAATCTATC 
     2041 GTTTTTCCTT GTCTGATGGC ACTCTTGTTG CTGCACAAAC GAAGAGCAAA CTCATCCGTT 
     2101 CTCAGACTAC TAATGAACCT CAACTTGTAA TATCTTTACA TATGCTTCAC AGAGAGCAGA 
     2161 ATGTGTGTGT GATGAATCCG GATCTGACTG GACAAACGAT GGGGAAGCCA CTGAATCCAA 
     2221 TTAGCTCTAA CAGCCCTGCC CATCAGGCCC TGTGCAGTGG GAACCCAGGT CAGGACATGA 
     2281 CCCTCAGTAG CAATATAAAT TTTCCCATAA ATGGCCCAAA GGAACAAATG GGCATGCCCA 
     2341 TGGGCAGGTT TGGTGGTTCT GGGGGAATGA ACCATGTGTC AGGCATGCAA GCAACCACTC 
     2401 CTCAGGGTAG TAACTATGCA CTCAAAATGA ACAGCCCCTC ACAAAGCAGC CCTGGCATGA 
     2461 ATCCAGGACA GCCCACCTCC ATGCTTTCAC CAAGGCATCG CATGAGCCCT GGAGTGGCTG 
     2521 GCAGCCCTCG AATCCCACCC AGTCAGTTTT CCCCTGCAGG AAGCTTGCAT TCCCCTGTGG 
     2581 GAGTTTGCAG CAGCACAGGA AATAGCCATA GTTATACCAA CAGCTCCCTC AATGCACTTC 
     2641 AGGCCCTCAG CGAGGGGCAC GGGGTCTCAT TAGGGTCATC GTTGGCTTCA CCAGACCTAA 
     2701 AAATGGGCAA TTTGCAAAAC TCCCCAGTTA ATATGAATCC TCCCCCACTC AGCAAGATGG 
     2761 GAAGCTTGGA CTCAAAAGAC TGTTTTGGAC TATATGGGGA GCCCTCTGAA GGTACAACTG 
     2821 GACAAGCAGA GAGCAGCTGC CATCCTGGAG AGCAAAAGGA AACAAATGAC CCCAACCTGC 
     2881 CCCCGGCCGT GAGCAGTGAG AGAGCTGACG GGCAGAGCAG ACTGCATGAC AGCAAAGGGC 
     2941 AGACCAAACT CCTGCAGCTG CTGACCACCA AATCTGATCA GATGGAGCCC TCGCCCTTAG 
     3001 CCAGCTCTTT GTCGGATACA AACAAAGACT CCACAGGTAG CTTGCCTGGT TCTGGGTCTA 
     3061 CACATGGAAC CTCGCTCAAG GAGAAGCATA AAATTTTGCA CAGACTCTTG CAGGACAGCA 
     3121 GTTCCCCTGT GGACTTGGCC AAGTTAACAG CAGAAGCCAC AGGCAAAGAC CTGAGCCAGG 
     3181 AGTCCAGCAG CACAGCTCCT GGATCAGAAG TGACTATTAA ACAAGAGCCG GTGAGCCCCA 
     3241 AGAAGAAAGA GAATGCACTA CTTCGCTATT TGCTAGATAA AGATGATACT AAAGATATTG 
     3301 GTTTACCAGA AATAACCCCC AAACTTGAGA GACTGGACAG TAAGACAGAT CCTGCCAGTA 
     3361 ACACAAAATT AATAGCAATG AAAACTGAGA AGGAGGAGAT GAGCTTTGAG CCTGGTGACC 
     3421 AGCCTGGCAG TGAGCTGGAC AACTTGGAGG AGATTTTGGA TGATTTGCAG AATAGTCAAT 
     3481 TACCACAGCT TTTCCCAGAC ACGAGGCCAG GCGCCCCTGC TGGATCAGTT GACAAGCAAG 
     3541 CCATCATCAA TGACCTCATG CAACTCACAG CTGAAAACAG CCCTGTCACA CCTGTTGGAG 
     3601 CCCAGAAAAC AGCACTGCGA ATTTCACAGA GCACTTTTAA TAACCCACGA CCAGGGCAAC 
     3661 TGGGCAGGTT ATTGCCAAAC CAGAATTTAC CACTTGACAT CACATTGCAA AGCCCAACTG 
     3721 GTGCTGGACC TTTCCCACCA ATCAGAAACA GTAGTCCCTA CTCAGTGATA CCTCAGCCAG 
     3781 GAATGATGGG TAATCAAGGG ATGATAGGAA ACCAAGGAAA TTTAGGGAAC AGTAGCACAG 
     3841 GAATGATTGG TAACAGTGCT TCTCGGCCTA CTATGCCATC TGGAGAATGG GCACCGCAGA 
     3901 GTTCGGCTGT GAGAGTCACC TGTGCTGCTA CCACCAGTGC CATGAACCGG CCAGTCCAAG 
     3961 GAGGTATGAT TCGGAACCCA GCAGCCAGCA TCCCCATGAG GCCCAGCAGC CAGCCTGGCC 
     4021 AAAGACAGAC GCTTCAGTCT CAGGTCATGA ATATAGGGCC ATCTGAATTA GAGATGAACA 
     4081 TGGGGGGACC TCAGTATAGC CAACAACAAG CTCCTCCAAA TCAGACTGCC CCATGGCCTG 
     4141 AAAGCATCCT GCCTATAGAC CAGGCGTCTT TTGCCAGCCA AAACAGGCAG CCATTTGGCA 
     4201 GTTCTCCAGA TGACTTGCTA TGTCCACATC CTGCAGCTGA GTCTCCGAGT GATGAGGGAG 
     4261 CTCTCCTGGA CCAGCTGTAT CTGGCCTTGC GGAATTTTGA TGGCCTGGAG GAGATTGATA 
     4321 GAGCCTTAGG AATACCCGAA CTGGTCAGCC AGAGCCAAGC AGTAGATCCA GAACAGTTCT 
     4381 CAAGTCAGGA TTCCAACATC ATGCTGGAGC AGAAGGCGCC CGTTTTCCCA CAGCAGTATG 
     4441 CATCTCAGGC ACAAATGGCC CAGGGTAGCT ATTCTCCCAT GCAAGATCCA AACTTTCACA 
     4501 CCATGGGACA GCGGCCTAGT TATGCCACAC TCCGTATGCA GCCCAGACCG GGCCTCAGGC 
     4561 CCACGGGCCT AGTGCAGAAC CAGCCAAATC AACTAAGACT TCAACTTCAG CATCGCCTCC 
     4621 AAGCACAGCA GAATCGCCAG CCACTTATGA ATCAAATCAG CAATGTTTCC AATGTGAACT 
     4681 TGACTCTGAG GCCTGGAGTA CCAACACAGG CACCTATTAA TGCACAGATG CTGGCCCAGA 
     4741 GACAGAGGGA AATCCTGAAC CAGCATCTTC GACAGAGACA AATGCATCAG CAACAGCAAG 
     4801 TTCAGCAACG AACTTTGATG ATGAGAGGAC AAGGGTTGAA TATGACACCA AGCATGGTGG 
     4861 CTCCTAGTGG TATCCCAGCA ACTATGAGCA ACCCTCGGAT TCCCCAGGCA AATGCACAGC 
     4921 AGTTTCCATT TCCTCCAAAC TACGGAATAA GTCAGCAACC TGATCCAGGC TTTACTGGGG 
     4981 CTACGACTCC CCAGAGCCCA CTTATGTCAC CCCGAATGGC ACATACACAG AGTCCCATGA 
     5041 TGCAACAGTC TCAGGCCAAC CCAGCCTATC AGGCCCCCTC CGACATAAAT GGATGGGCGC 
     5101 AGGGGAACAT GGGCGGAAAC AGCATGTTTT CCCAGCAGTC CCCACCACAC TTTGGGCAGC 
     5161 AAGCAAACAC CAGCATGTAC AGTAACAACA TGAACATCAA TGTGTCCATG GCGACCAACA 
     5221 CAGGTGGCAT GAGCAGCATG AACCAGATGA CAGGACAGAT CAGCATGACC TCAGTGACCT 
     5281 CCGTGCCTAC GTCAGGGCTG TCCTCCATGG GTCCCGAGCA GGTTAATGAT CCTGCTCTGA 
     5341 GGGGAGGCAA CCTGTTCCCA AACCAGCTGC CTGGAATGGA TATGATTAAG CAGGAGGGAG 
     5401 ACACAACACG GAAATATTGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5461 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5521 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5581 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5641 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5701 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5761 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5821 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5881 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5941 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6001 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6061 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6121 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6181 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6241 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6301 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6361 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6421 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6481 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6541 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6601 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6661 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6721 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6781 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6841 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6901 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6961 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7021 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7081 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7141 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7201 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7261 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7321 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7381 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7441 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7501 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7561 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7621 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7681 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7741 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7801 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7861 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7921 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7981 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8041 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8101 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8161 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8221 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8281 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8341 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8401 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8461 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8521 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8701 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8881 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8941 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9001 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9061 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9121 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9181 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9241 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//