LOCUS       RC212176                8445 bp    DNA     circular  SYN 
DEFINITION  INPP5D (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase, 145kDa (INPP5D), transcript variant 2
ACCESSION   NM_005541
VERSION     NM_005541.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4602..4693 
                     /label="Myc-DDK"  
     misc_feature    4723..5308 
                     /label="PolyA signal"  
     misc_feature    4780..4799 
                     /label="Restriction Enzyme"  
     misc_feature    5584..6256 
                     /label="ColE1"  
     misc_feature    6686..7480 
                     /label="Kan/Neo"  
     misc_feature    7515..7817 
                     /label="SV40 ori"  
     misc_feature    7903..8380 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4584..4590 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4584 
                     /label="INPP5D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCCCTGC TGGAACCATG GCAACATCAC CCGCTCCAAG GCGGAGGAGC 
     1081 TGCTTTCCAG GACAGGCAAG GACGGGAGCT TCCTCGTGCG TGCCAGCGAG TCCATCTCCC 
     1141 GGGCATACGC GCTCTGCGTG CTGTATCGGA ATTGCGTTTA CACTTACAGA ATTCTGCCCA 
     1201 ATGAAGATGA TAAATTCACT GTTCAGGCAT CCGAAGGCGT CTCCATGAGG TTCTTCACCA 
     1261 AGCTGGACCA GCTCATCGAG TTTTACAAGA AGGAAAACAT GGGGCTGGTG ACCCATCTGC 
     1321 AATACCCTGT GCCGCTGGAG GAAGAGGACA CAGGCGACGA CCCTGAGGAG GACACAGAAA 
     1381 GTGTCGTGTC TCCACCCGAG CTGCCCCCAA GAAACATCCC GCTGACTGCC AGCTCCTGTG 
     1441 AGGCCAAGGA GGTTCCTTTT TCAAACGAGA ATCCCCGAGC GACCGAGACC AGCCGGCCGA 
     1501 GCCTCTCCGA GACATTGTTC CAGCGACTGC AAAGCATGGA CACCAGTGGG CTTCCAGAAG 
     1561 AGCATCTTAA GGCCATCCAA GATTATTTAA GCACTCAGCT CGCCCAGGAC TCTGAATTTG 
     1621 TGAAGACAGG GTCCAGCAGT CTTCCTCACC TGAAGAAACT GACCACACTG CTCTGCAAGG 
     1681 AGCTCTATGG AGAAGTCATC CGGACCCTCC CATCCCTGGA GTCTCTGCAG AGGTTATTTG 
     1741 ACCAGCAGCT CTCCCCGGGC CTCCGTCCAC GTCCTCAGGT TCCTGGTGAG GCCAATCCCA 
     1801 TCAACATGGT GTCCAAGCTC AGCCAACTGA CAAGCCTGTT GTCATCCATT GAAGACAAGG 
     1861 TCAAGGCCTT GCTGCACGAG GGTCCTGAGT CTCCGCACCG GCCCTCCCTT ATCCCTCCAG 
     1921 TCACCTTTGA GGTGAAGGCA GAGTCTCTGG GGATTCCTCA GAAAATGCAG CTCAAAGTCG 
     1981 ACGTTGAGTC TGGGAAACTG ATCATTAAGA AGTCCAAGGA TGGTTCTGAG GACAAGTTCT 
     2041 ACAGCCACAA GAAAATCCTG CAGCTCATTA AGTCACAGAA ATTTCTGAAT AAGTTGGTGA 
     2101 TCTTGGTGGA AACAGAGAAG GAGAAGATCC TGCGGAAGGA ATATGTTTTT GCTGACTCCA 
     2161 AAAAGAGAGA AGGCTTCTGC CAGCTCCTGC AGCAGATGAA GAACAAGCAC TCAGAGCAGC 
     2221 CGGAGCCCGA CATGATCACC ATCTTCATCG GCACCTGGAA CATGGGTAAC GCCCCCCCTC 
     2281 CCAAGAAGAT CACGTCCTGG TTTCTCTCCA AGGGGCAGGG AAAGACGCGG GACGACTCTG 
     2341 CGGACTACAT CCCCCATGAC ATTTACGTGA TCGGCACCCA AGAGGACCCC CTGAGTGAGA 
     2401 AGGAGTGGCT GGAGATCCTC AAACACTCCC TGCAAGAAAT CACCAGTGTG ACTTTTAAAA 
     2461 CAGTCGCCAT CCACACGCTC TGGAACATCC GCATCGTGGT GCTGGCCAAG CCTGAGCACG 
     2521 AGAACCGGAT CAGCCACATC TGTACTGACA ACGTGAAGAC AGGCATTGCA AACACACTGG 
     2581 GGAACAAGGG AGCCGTGGGG GTGTCGTTCA TGTTCAATGG AACCTCCTTA GGGTTCGTCA 
     2641 ACAGCCACTT GACTTCAGGA AGTGAAAAGA AACTCAGGCG AAACCAAAAC TATATGAACA 
     2701 TTCTCCGGTT CCTGGCCCTG GGCGACAAGA AGCTGAGTCC CTTTAACATC ACTCACCGCT 
     2761 TCACGCACCT CTTCTGGTTT GGGGATCTTA ACTACCGTGT GGATCTGCCT ACCTGGGAGG 
     2821 CAGAAACCAT CATCCAGAAA ATCAAGCAGC AGCAGTACGC AGACCTCCTG TCCCACGACC 
     2881 AGCTGCTCAC AGAGAGGAGG GAGCAGAAGG TCTTCCTACA CTTCGAGGAG GAAGAAATCA 
     2941 CGTTTGCCCC AACCTACCGT TTTGAGAGAC TGACTCGGGA CAAATACGCC TACACCAAGC 
     3001 AGAAAGCGAC AGGGATGAAG TACAACTTGC CTTCCTGGTG TGACCGAGTC CTCTGGAAGT 
     3061 CTTATCCCCT GGTGCACGTG GTGTGTCAGT CTTATGGCAG TACCAGCGAC ATCATGACGA 
     3121 GTGACCACAG CCCTGTCTTT GCCACATTTG AGGCAGGAGT CACTTCCCAG TTTGTCTCCA 
     3181 AGAACGGTCC CGGGACTGTT GACAGCCAAG GACAGATTGA GTTTCTCAGG TGCTATGCCA 
     3241 CATTGAAGAC CAAGTCCCAG ACCAAATTCT ACCTGGAGTT CCACTCGAGC TGCTTGGAGA 
     3301 GTTTTGTCAA GAGTCAGGAA GGAGAAAATG AAGAAGGAAG TGAGGGGGAG CTGGTGGTGA 
     3361 AGTTTGGTGA GACTCTTCCA AAGCTGAAGC CCATTATCTC TGACCCTGAG TACCTGCTAG 
     3421 ACCAGCACAT CCTCATCAGC ATCAAGTCCT CTGACAGCGA CGAATCCTAT GGCGAGGGCT 
     3481 GCATTGCCCT TCGGTTAGAG GCCACAGAAA CGCAGCTGCC CATCTACACG CCTCTCACCC 
     3541 ACCATGGGGA GTTGACAGGC CACTTCCAGG GGGAGATCAA GCTGCAGACC TCTCAGGGCA 
     3601 AGACGAGGGA GAAGCTCTAT GACTTTGTGA AGACGGAGCG TGATGAATCC AGTGGGCCAA 
     3661 AGACCCTGAA GAGCCTCACC AGCCACGACC CCATGAAGCA GTGGGAAGTC ACTAGCAGGG 
     3721 CCCCTCCGTG CAGTGGCTCC AGCATCACTG AAATCATCAA CCCCAACTAC ATGGGAGTGG 
     3781 GGCCCTTTGG GCCACCAATG CCCCTGCACG TGAAGCAGAC CTTGTCCCCT GACCAGCAGC 
     3841 CCACAGCCTG GAGCTACGAC CAGCCGCCCA AGGACTCCCC GCTGGGGCCC TGCAGGGGAG 
     3901 AAAGTCCTCC GACACCTCCC GGCCAGCCGC CCATATCACC CAAGAAGTTT TTACCCTCAA 
     3961 CAGCAAACCG GGGTCTCCCT CCCAGGACAC AGGAGTCAAG GCCCAGTGAC CTGGGGAAGA 
     4021 ACGCAGGGGA CACGCTGCCT CAGGAGGACC TGCCGCTGAC GAAGCCCGAG ATGTTTGAGA 
     4081 ACCCCCTGTA TGGGTCCCTG AGTTCCTTCC CTAAGCCTGC TCCCAGGAAG GACCAGGAAT 
     4141 CCCCCAAAAT GCCGCGGAAG GAACCCCCGC CCTGCCCGGA ACCCGGCATC TTGTCGCCCA 
     4201 GCATCGTGCT CACCAAAGCC CAGGAGGCTG ATCGCGGCGA GGGGCCCGGC AAGCAGGTGC 
     4261 CCGCGCCCCG GCTGCGCTCC TTCACGTGCT CATCCTCTGC CGAGGGCAGG GCGGCCGGCG 
     4321 GGGACAAGAG CCAAGGGAAG CCCAAGACCC CGGTCAGCTC CCAGGCCCCG GTGCCGGCCA 
     4381 AGAGGCCCAT CAAGCCTTCC AGATCGGAAA TCAACCAGCA GACCCCGCCC ACCCCGACGC 
     4441 CGCGGCCGCC GCTGCCAGTC AAGAGCCCGG CGGTGCTGCA CCTCCAGCAC TCCAAGGGCC 
     4501 GCGACTACCG CGACAACACC GAGCTCCCGC ATCACGGCAA GCACCGGCCG GAGGAGGGGC 
     4561 CACCAGGGCC TCTAGGCAGG ACTGCCATGC AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4621 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4681 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5941 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6241 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6301 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6361 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6421 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6481 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6541 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6601 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6661 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6721 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6781 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6841 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6901 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6961 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7021 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7081 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7141 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7201 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7261 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7321 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7381 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7441 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7501 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7561 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7621 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7681 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7861 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7921 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7981 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8041 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8101 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8161 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8221 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8281 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8341 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8401 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//