LOCUS       RC212168                9024 bp    DNA     circular  SYN 
DEFINITION  HEG1 (Myc-DDK-tagged)-Human HEG homolog 1 (zebrafish) (HEG1)
ACCESSION   NM_020733
VERSION     NM_020733.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5181..5272 
                     /label="Myc-DDK"  
     misc_feature    5302..5887 
                     /label="PolyA signal"  
     misc_feature    5359..5378 
                     /label="Restriction Enzyme"  
     misc_feature    6163..6835 
                     /label="ColE1"  
     misc_feature    7265..8059 
                     /label="Kan/Neo"  
     misc_feature    8094..8396 
                     /label="SV40 ori"  
     misc_feature    8482..8959 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5163..5169 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5163 
                     /label="HEG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGCCG CGCGCCTCGC GGTGGCCGCC GCCGCTCCTG CTGCTGTTGC 
     1081 TGCCGCTGCT GCTGCTGCCG CCGGCGGCCC CCGGGACGCG GGACCCGCCG CCTTCCCCGG 
     1141 CTCGCCGCGC GCTGAGCCTG GCGCCCCTCG CGGGAGCGGG GCTGGAGCTG CAGCTGGAGC 
     1201 GCCGCCCGGA GCGCGAGCCG CCGCCCACGC CGCCCCGGGA GCGCCGCGGG CCCGCGACCC 
     1261 CCGGCCCCAG CTACAGGGCC CCTGAGCCAG GCGCCGCGAC ACAGCGGGGA CCCTCCGGCC 
     1321 GGGCCCCCAG AGGCGGGAGC GCGGATGCTG CCTGGAAACA TTGGCCAGAA AGTAACACTG 
     1381 AGGCCCATGT AGAAAACATC ACCTTCTATC AGAATCAAGA GGACTTTTCA ACAGTGTCCT 
     1441 CCAAAGAGGG CGTGATGGTT CAGACCTCTG GGAAGAGCCA TGCTGCTTCG GATGCTCCAG 
     1501 AAAACCTCAC TCTACTCGCT GAAACAGCAG ATGCTAGAGG AAGGAGCGGC TCTTCAAGTA 
     1561 GAACAAACTT CACCATTTTG CCTGTTGGGT ACTCACTGGA GATAGCAACA GCTCTGACTT 
     1621 CCCAGAGTGG CAACTTAGCC TCAGAAAGTC TTCACCTGCC ATCCAGCAGT TCAGAGTTCG 
     1681 ATGAAAGAAT TGCCGCTTTT CAAACAAAGA GTGGAACAGC CTCGGAGATG GGAACAGAGA 
     1741 GGGCGATGGG GCTGTCAGAA GAATGGACTG TGCACAGCCA AGAGGCCACC ACTTCGGCTT 
     1801 GGAGCCCGTC CTTTCTTCCT GCTTTGGAGA TGGGAGAGCT GACCACGCCT TCTAGGAAGA 
     1861 GAAATTCCTC AGGACCAGAT CTCTCCTGGC TGCATTTCTA CAGGACAGCA GCTTCCTCTC 
     1921 CTCTCTTAGA CCTTTCCTCA TCTTCTGAAA GTACAGAGAA GCTTAACAAC TCCACTGGCC 
     1981 TCCAGAGCTC CTCAGTCAGT CAAACAAAGA CAATGCATGT TGCCACCGTG TTCACTGATG 
     2041 GTGGCCCGAG AACGCTGCGA TCTTTGACGG TCAGTCTGGG ACCTGTGAGC AAGACAGAAG 
     2101 GCTTCCCCAA GGACTCCAGA ATTGCCACGA CTTCATCCTC AGTCCTTCTT TCACCCTCTG 
     2161 CAGTGGAATC GAGAAGAAAC AGTAGAGTAA CTGGGAATCC AGGGGATGAG GAATTCATTG 
     2221 AACCATCCAC AGAAAATGAA TTTGGACTTA CGTCTTTGCG TTGGCAAAAT GATTCCCCAA 
     2281 CCTTTGGAGA ACATCAGCTT GCCAGCAGCT CTGAGGTGCA AAATGGAAGT CCCATGTCTC 
     2341 AGACTGAGAC TGTGTCTAGG TCAGTCGCAC CCATGAGAGG TGGAGAGATC ACTGCACACT 
     2401 GGCTCTTGAC CAACAGCACA ACATCTGCAG ATGTGACAGG AAGCTCTGCT TCATATCCTG 
     2461 AAGGTGTGAA TGCTTCAGTG TTGACCCAGT TCTCAGACTC TACTGTACAG TCTGGAGGAA 
     2521 GTCACACAGC ATTGGGAGAT AGGAGTTATT CAGAGTCTTC ATCTACATCT TCCTCGGAAA 
     2581 GCTTGAATTC ATCAGCACCA CGTGGAGAAC GTTCGATCGC TGGGATTAGC TACGGTCAAG 
     2641 TGCGTGGCAC AGCTATTGAA CAAAGGACTT CCAGCGACCA CACAGACCAC ACCTACCTGT 
     2701 CATCTACTTT CACCAAAGGA GAACGGGCGT TACTGTCCAT TACAGATAAC AGTTCATCCT 
     2761 CAGACATTGT GGAGAGCTCA ACTTCTTATA TTAAAATCTC AAACTCTTCA CATTCAGAGT 
     2821 ATTCCTCCTT TTTTCATGCT CAGACTGAGA GAAGTAACAT CTCATCCTAT GACGGGGAAT 
     2881 ATGCTCAGCC TTCTACTGAG TCGCCAGTTC TGCATACATC CAACCTTCCG TCCTACACAC 
     2941 CCACCATTAA TATGCCGAAC ACTTCGGTTG TTCTGGACAC TGATGCTGAG TTTGTTAGTG 
     3001 ACTCCTCCTC CTCCTCTTCC TCCTCCTCCT CTTCTTCTTC TTCAGGGCCT CCTTTGCCTC 
     3061 TGCCCTCTGT GTCACAATCC CACCATTTAT TTTCATCAAT TTTACCATCA ACCAGGGCCT 
     3121 CTGTGCATCT ACTAAAGTCT ACCTCTGATG CATCCACACC ATGGTCTTCC TCACCATCAC 
     3181 CTTTACCAGT ATCCTTAACG ACATCTACAT CTGCCCCACT TTCTGTCTCA CAAACAACCT 
     3241 TGCCACAGTC ATCTTCTACC CCTGTCCTGC CCAGGGCAAG GGAGACTCCT GTGACTTCAT 
     3301 TTCAGACATC AACAATGACA TCATTCATGA CAATGCTCCA TAGTAGTCAA ACTGCAGACC 
     3361 TTAAGAGCCA GAGCACCCCA CACCAAGAGA AAGTCATTAC AGAATCAAAG TCACCAAGCC 
     3421 TGGTGTCTCT GCCCACAGAG TCCACCAAAG CTGTAACAAC AAACTCTCCT TTGCCTCCAT 
     3481 CCTTAACAGA GTCCTCCACA GAGCAAACCC TTCCAGCCAC AAGCACCAAC TTAGCACAAA 
     3541 TGTCTCCAAC TTTCACAACT ACCATTCTGA AGACCTCTCA GCCTCTTATG ACCACTCCTG 
     3601 GCACCCTGTC AAGCACAGCA TCTCTGGTCA CTGGCCCTAT AGCCGTACAG ACTACAGCTG 
     3661 GAAAACAGCT CTCGCTGACC CATCCTGAAA TACTAGTTCC TCAAATCTCA ACAGAAGGTG 
     3721 GCATCAGCAC AGAAAGGAAC CGAGTGATTG TGGATGCTAC CACTGGATTG ATCCCTTTGA 
     3781 CCAGTGTACC CACATCAGCA AAAGAAATGA CCACAAAGCT TGGCGTTACA GCAGAGTACA 
     3841 GCCCAGCTTC ACGTTCCCTC GGAACATCTC CTTCTCCCCA AACCACAGTT GTTTCCACGG 
     3901 CTGAAGACTT GGCTCCCAAA TCTGCCACCT TTGCTGTTCA GAGCAGCACA CAGTCACCAA 
     3961 CAACAGTGTC CTCTTCAGCC TCAGTCAACA GCTGTGCTGT GAACCCTTGT CTTCACAATG 
     4021 GCGAATGCGT CGCAGACAAC ACCAGCCGTG GCTACCACTG CAGGTGCCCG CCTTCCTGGC 
     4081 AAGGGGATGA TTGCAGTGTG GATGTGAATG AGTGCCTGTC GAACCCCTGC CCATCCACAG 
     4141 CCATGTGCAA CAATACTCAG GGATCCTTTA TCTGCAAATG CCCGGTTGGG TACCAGTTGG 
     4201 AAAAAGGGAT ATGCAATTTG GTTAGAACCT TCGTGACAGA GTTTAAATTA AAGAGAACTT 
     4261 TTCTTAATAC AACTGTGGAA AAACATTCAG ACCTACAAGA AGTTGAAAAT GAGATCACCA 
     4321 AAACGTTAAA TATGTGTTTT TCAGCGTTAC CTAGTTACAT CCGATCTACA GTTCACGCCT 
     4381 CTAGGGAGTC CAACGCGGTG GTGATCTCAC TGCAAACAAC CTTTTCCCTG GCCTCCAATG 
     4441 TGACGCTATT TGACCTGGCT GATAGGATGC AGAAATGTGT CAACTCCTGC AAGTCCTCTG 
     4501 CTGAGGTCTG CCAGCTCTTG GGATCTCAGA GGCGGATCTT TAGAGCGGGC AGCTTGTGCA 
     4561 AGCGGAAGAG TCCCGAATGT GACAAAGACA CCTCCATCTG CACTGACCTG GACGGCGTTG 
     4621 CCCTGTGCCA GTGCAAGTCG GGATACTTTC AGTTCAACAA GATGGACCAC TCCTGCCGAG 
     4681 CATGTGAAGA TGGATATAGG CTTGAAAATG AAACCTGCAT GAGTTGCCCA TTTGGCCTTG 
     4741 GTGGTCTCAA CTGTGGAAAC CCCTATCAGC TTATCACTGT GGTGATCGCA GCCGCGGGAG 
     4801 GTGGGCTCCT GCTCATCCTA GGCATCGCAC TGATTGTTAC CTGTTGCAGA AAGAATAAAA 
     4861 ATGACATAAG CAAACTCATC TTCAAAAGTG GAGATTTCCA AATGTCCCCG TATGCTGAAT 
     4921 ACCCCAAAAA TCCTCGCTCA CAAGAATGGG GCCGAGAAGC TATTGAAATG CATGAGAATG 
     4981 GAAGTACCAA AAACCTCCTC CAGATGACGG ATGTGTACTA CTCGCCTACA AGTGTAAGGA 
     5041 ATCCAGAACT TGAACGAAAC GGACTCTACC CGGCCTACAC TGGACTGCCA GGATCACGGC 
     5101 ATTCTTGCAT TTTCCCCGGA CAGTATAACC CGTCTTTCAT CAGTGATGAA AGCAGAAGAA 
     5161 GAGACTACTT TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5221 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5281 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5341 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5401 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5461 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5521 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5581 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5641 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5701 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5761 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5821 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5881 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5941 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6001 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6061 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6121 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6181 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6241 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6301 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6361 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6421 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6481 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6541 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6601 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6661 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6721 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6781 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6841 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6901 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6961 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7021 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7081 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7141 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7201 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7261 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7321 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7381 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7441 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7501 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7561 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7621 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7681 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7741 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7801 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7861 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7921 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7981 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8041 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8101 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8161 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8221 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8281 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8341 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8401 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8461 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8521 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8581 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8641 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8701 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8761 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8821 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8881 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8941 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9001 TAACAAAAAT TTAACGCGAA TTTT
//