LOCUS       RC212154                8202 bp    DNA     circular  SYN 
DEFINITION  KCNH8 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 8 (KCNH8)
ACCESSION   NM_144633
VERSION     NM_144633.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4359..4450 
                     /label="Myc-DDK"  
     misc_feature    4480..5065 
                     /label="PolyA signal"  
     misc_feature    4537..4556 
                     /label="Restriction Enzyme"  
     misc_feature    5341..6013 
                     /label="ColE1"  
     misc_feature    6443..7237 
                     /label="Kan/Neo"  
     misc_feature    7272..7574 
                     /label="SV40 ori"  
     misc_feature    7660..8137 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4341..4347 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4341 
                     /label="KCNH8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTTATG AAAGGATTAC TGGCGCCGCA AAACACCTTC CTGGACACCA 
     1081 TCGCCACCCG TTTTGACGGA ACACATAGCA ACTTCATCCT TGCCAATGCC CAGGTGGCTA 
     1141 AGGGTTTCCC CATAGTCTAC TGTTCCGATG GCTTCTGCGA GCTTGCTGGA TTTGCCCGAA 
     1201 CTGAAGTCAT GCAGAAGAGT TGTAGCTGCA AGTTCTTATT TGGGGTTGAA ACCAATGAGC 
     1261 AACTGATGCT TCAAATAGAA AAGTCACTGG AGGAGAAAAC AGAATTCAAA GGAGAAATTA 
     1321 TGTTCTACAA GAAAAACGGG TCTCCATTTT GGTGCCTACT GGATATTGTT CCCATAAAGA 
     1381 ATGAAAAAGG AGATGTAGTA CTTTTTCTGG CCTCGTTCAA AGATATAACA GATACAAAAG 
     1441 TGAAGATTAC TCCAGAAGAT AAAAAAGAAG ACAAAGTCAA AGGAAGATCA AGAGCAGGGA 
     1501 CCCACTTTGA CTCAGCCCGG AGACGGAGTC GAGCAGTCCT TTATCACATC TCTGGGCACC 
     1561 TGCAAAGAAG AGAAAAGAAC AAATTGAAAA TAAATAACAA TGTTTTTGTA GATAAACCAG 
     1621 CATTTCCGGA GTATAAAGTT TCTGATGCAA AAAAGTCCAA ATTCATACTT CTGCATTTTA 
     1681 GCACTTTTAA AGCTGGCTGG GACTGGCTTA TTTTGTTGGC AACGTTTTAT GTTGCTGTGA 
     1741 CTGTACCTTA CAACGTTTGC TTTATTGGCA ATGACGACCT GTCCACAACT CGGAGCACAA 
     1801 CCGTCAGTGA CATTGCAGTG GAGATTCTTT TTATTATAGA TATTATTTTA AATTTCCGAA 
     1861 CAACTTATGT CAGCAAGTCT GGCCAAGTTA TCTTTGAAGC AAGATCAATT TGCATCCACT 
     1921 ATGTCACAAC CTGGTTCATC ATTGATTTAA TCGCTGCCCT GCCTTTTGAT CTTCTGTATG 
     1981 CTTTCAACGT CACAGTGGTG TCTCTCGTGC ATCTTCTAAA GACAGTGCGC CTCTTGCGTC 
     2041 TTTTGCGTCT GCTGCAGAAG TTAGACCGCT ATTCCCAACA CAGTACTATC GTCCTGACTC 
     2101 TGCTCATGTC CATGTTTGCA CTCCTTGCAC ACTGGATGGC GTGTATCTGG TACGTCATTG 
     2161 GAAAAATGGA GAGGGAAGAC AACAGCCTTC TGAAGTGGGA AGTTGGTTGG CTTCATGAGT 
     2221 TGGGAAAGAG ACTGGAATCT CCATACTATG GCAACAATAC CTTGGGGGGC CCGTCGATCC 
     2281 GAAGTGCCTA TATTGCCGCT CTGTACTTCA CGCTGAGCAG CCTCACCAGC GTGGGTTTTG 
     2341 GGAACGTCTC TGCTAATACA GATGCAGAAA AGATCTTCTC CATCTGCACC ATGCTGATTG 
     2401 GTGCCTTGAT GCACGCCTTG GTGTTTGGAA ACGTGACAGC AATCATACAG AGGATGTACT 
     2461 CCAGATGGTC CCTCTATCAC ACTAGAACTA AGGATCTGAA AGATTTCATC CGTGTCCATC 
     2521 ACTTGCCCCA ACAACTCAAG CAGAGGATGC TCGAATATTT TCAAACAACC TGGTCAGTCA 
     2581 ACAATGGAAT AGATTCAAAT GAGCTTTTGA AAGACTTTCC AGATGAACTG CGTTCTGACA 
     2641 TCACTATGCA CTTGAACAAG GAGATCTTAC AGTTGTCCCT TTTTGAATGT GCCAGCCGGG 
     2701 GCTGCCTCAG GTCTCTGTCT CTACACATCA AAACCTCTTT CTGTGCTCCG GGGGAGTATC 
     2761 TGCTGCGTCA AGGGGATGCT TTGCAGGCCA TCTACTTTGT ATGCTCGGGC TCCATGGAAG 
     2821 TTCTTAAAGA CAGCATGGTG CTGGCTATTC TTGGGAAAGG GGATTTAATT GGAGCAAATC 
     2881 TATCAATTAA GGACCAAGTG ATCAAGACCA ATGCAGATGT AAAGGCTTTA ACCTACTGTG 
     2941 ATCTCCAGTG TATCATCCTC AAAGGACTCT TTGAAGTGCT AGACCTTTAC CCAGAATATG 
     3001 CTCACAAATT CGTGGAAGAC ATTCAGCATG ACCTCACATA CAACCTCCGA GAAGGTCATG 
     3061 AGAGTGATGT GATATCAAGA CTATCAAACA AATCTATGGT CTCACAGTCA GAGCCCAAGG 
     3121 GAAATGGCAA CATCAACAAG CGACTCCCAT CCATTGTGGA AGATGAGGAA GAGGAGGAGG 
     3181 AGGGGGAGGA AGAGGAGGCA GTCTCCCTCT CTCCCATCTG CACAAGGGGA TCTTCTTCGC 
     3241 GCAACAAGAA GGTTGGAAGC AATAAAGCCT ACCTGGGCTT AAGCTTAAAG CAACTGGCCT 
     3301 CGGGAACGGT GCCCTTTCAC TCGCCTATCA GAGTCTCCAG GTCAAATTCC CCCAAAACCA 
     3361 AGCAGGAAAT TGACCCCCCC AACCATAATA AAAGGAAAGA GAAGAACTTG AAATTGCAAC 
     3421 TTTCAACTTT GAATAATGCT GGACCCCCAG ACCTCAGTCC AAGGATTGTT GATGGAATTG 
     3481 AAGATGGAAA CAGCAGTGAA GAAAGTCAGA CTTTTGATTT TGGCTCTGAA CGAATCAGAT 
     3541 CAGAGCCCAG AATTTCTCCT CCTCTTGGAG ATCCAGAGAT TGGAGCTGCT GTTCTCTTCA 
     3601 TCAAAGCAGA GGAGACCAAG CAGCAGATAA ACAAACTCAA CAGTGAGGTA ACAACATTGA 
     3661 CTCAGGAAGT TTCTCAGTTG GGTAAAGACA TGAGAAATGT GATCCAGCTT CTGGAAAACG 
     3721 TTCTGTCACC TCAGCAGCCA TCACGGTTTT GCTCTTTGCA CAGCACCTCT GTGTGTCCCT 
     3781 CCAGGGAGAG CTTACAGACC AGAACGAGCT GGAGTGCACA CCAGCCTTGC CTACACTTGC 
     3841 AAACAGGCGG GGCTGCTTAT ACCCAAGCAC AACTTTGTAG CAGTAATATC ACCTCAGACA 
     3901 TTTGGAGTGT GGATCCCTCC TCTGTGGGGA GCAGCCCCCA ACGAACTGGA GCTCATGAGC 
     3961 AAAATCCTGC AGACAGTGAA CTTTATCATT CTCCAAGCCT TGATTATTCA CCTTCCCACT 
     4021 ACCAGGTTGT CCAAGAAGGT CATTTGCAAT TTTTAAGGTG CATCTCTCCA CATTCAGATT 
     4081 CTACGTTGAC GCCTCTGCAG TCCATTTCAG CAACTCTCTC ATCTTCTGTC TGCTCCTCTT 
     4141 CGGAAACATC TTTGCACCTA GTTCTCCCAA GCAGATCAGA GGAGGGCAGC TTCAGTCAGG 
     4201 GAACTGTGAG TTCCTTCAGT CTGGAAAACT TACCAGGATC TTGGAACCAG GAAGGAATGG 
     4261 CATCAGCTTC TACAAAACCT TTGGAGAACC TTCCACTGGA AGTTGTCACA AGCACAGCAG 
     4321 AAGTGAAAGA TAACAAAGCC ATAAATGTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4381 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4441 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4501 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4561 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4621 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4681 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4741 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4801 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4861 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4921 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4981 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5041 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5101 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5161 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5221 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5281 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5341 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5401 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5461 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5521 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5581 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5641 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5701 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5761 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5821 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5881 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5941 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6001 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6061 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6121 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6181 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6241 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6301 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6361 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6421 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6481 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6541 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6601 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6661 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6721 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6781 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6841 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6901 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6961 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7021 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7081 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7141 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7201 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7261 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7321 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7381 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7441 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7501 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7621 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7681 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7741 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7801 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7861 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7921 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7981 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8041 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8101 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8161 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//