LOCUS       RC212107L1                9024 bp    DNA     circular  SYN 
DEFINITION  GRIA2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, AMPA 2 (GRIA2), transcript variant 1
ACCESSION   NM_000826
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7315..7495 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8223..8882 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7654..8222 
                     /label="ColE1"  
     misc_feature    6190..6210 
                     /label="LR50 primer"  
     misc_feature    5987..6058 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6199..6790 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5969..5975 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5969 
                     /label="GRIA2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCAAAAGATT ATGCATATTT CTGTCCTCCT 
     3361 TTCTCCTGTT TTATGGGGAC TGATTTTTGG TGTCTCTTCT AACAGCATAC AGATAGGGGG 
     3421 GCTATTTCCT AGGGGCGCCG ATCAAGAATA CAGTGCATTT CGAGTAGGGA TGGTTCAGTT 
     3481 TTCCACTTCG GAGTTCAGAC TGACACCCCA CATCGACAAT TTGGAGGTGG CAAACAGCTT 
     3541 CGCAGTCACT AATGCTTTCT GCTCCCAGTT TTCGAGAGGA GTCTATGCTA TTTTTGGATT 
     3601 TTATGACAAG AAGTCTGTAA ATACCATCAC ATCATTTTGC GGAACACTCC ACGTCTCCTT 
     3661 CATCACTCCC AGCTTCCCAA CAGATGGCAC ACATCCATTT GTCATTCAGA TGAGACCCGA 
     3721 CCTCAAAGGA GCTCTCCTTA GCTTGATTGA ATACTATCAA TGGGACAAGT TTGCATACCT 
     3781 CTATGACAGT GACAGAGGCT TATCAACACT GCAAGCTGTG CTGGATTCTG CTGCTGAAAA 
     3841 GAAATGGCAA GTGACTGCTA TCAATGTGGG AAACATTAAC AATGACAAGA AAGATGAGAT 
     3901 GTACCGATCA CTTTTTCAAG ATCTGGAGTT AAAAAAGGAA CGGCGTGTAA TTCTGGACTG 
     3961 TGAAAGGGAT AAAGTAAACG ACATTGTAGA CCAGGTTATT ACCATTGGAA AACATGTTAA 
     4021 AGGGTACCAC TACATCATTG CAAATCTGGG ATTTACTGAT GGAGACCTAT TAAAAATCCA 
     4081 GTTTGGAGGT GCAAATGTCT CTGGATTTCA GATAGTGGAC TATGATGATT CGTTGGTATC 
     4141 TAAATTTATA GAAAGATGGT CAACACTGGA AGAAAAAGAA TACCCTGGAG CTCACACAAC 
     4201 AACAATTAAG TATACTTCTG CTCTGACCTA TGATGCCGTT CAAGTGATGA CTGAAGCCTT 
     4261 CCGCAACCTA AGGAAGCAAA GAATTGAAAT CTCCCGAAGG GGGAATGCAG GAGACTGTCT 
     4321 GGCAAACCCA GCAGTGCCCT GGGGACAAGG TGTAGAAATA GAAAGGGCCC TCAAACAGGT 
     4381 TCAGGTTGAA GGTCTCTCAG GAAATATAAA GTTTGACCAG AATGGAAAAA GAATAAACTA 
     4441 TACAATTAAC ATCATGGAGC TCAAAACTAA TGGGCCCCGG AAGATTGGCT ACTGGAGTGA 
     4501 AGTGGACAAA ATGGTTGTTA CCCTTACTGA GCTCCCTTCT GGAAATGACA CCTCTGGGCT 
     4561 TGAGAATAAG ACTGTTGTTG TCACCACAAT TTTGGAATCT CCGTATGTTA TGATGAAGAA 
     4621 AAATCATGAA ATGCTTGAAG GCAATGAGCG CTATGAGGGC TACTGTGTTG ACCTGGCTGC 
     4681 AGAAATCGCC AAACATTGTG GGTTCAAGTA CAAGTTGACA ATTGTTGGTG ATGGCAAGTA 
     4741 TGGGGCCAGG GATGCAGACA CGAAAATTTG GAATGGGATG GTTGGAGAAC TTGTATATGG 
     4801 GAAAGCTGAT ATTGCAATTG CTCCATTAAC TATTACCCTT GTGAGAGAAG AGGTGATTGA 
     4861 CTTCTCAAAG CCCTTCATGA GCCTCGGGAT ATCTATCATG ATCAAGAAGC CTCAGAAGTC 
     4921 CAAACCAGGA GTGTTTTCCT TTCTTGATCC TTTAGCCTAT GAGATCTGGA TGTGCATTGT 
     4981 TTTTGCCTAC ATTGGGGTCA GTGTAGTTTT ATTCCTGGTC AGCAGATTTA GCCCCTACGA 
     5041 GTGGCACACT GAGGAGTTTG AAGATGGAAG AGAAACACAA AGTAGTGAAT CAACTAATGA 
     5101 ATTTGGGATT TTTAATAGTC TCTGGTTTTC CTTGGGTGCC TTTATGCGGC AAGGATGCGA 
     5161 TATTTCGCCA AGATCCCTCT CTGGGCGCAT TGTTGGAGGT GTGTGGTGGT TCTTTACCCT 
     5221 GATCATAATC TCCTCCTACA CGGCTAACTT AGCTGCCTTC CTGACTGTAG AGAGGATGGT 
     5281 GTCTCCCATC GAAAGTGCTG AGGATCTTTC TAAGCAAACA GAAATTGCTT ATGGAACATT 
     5341 AGACTCTGGC TCCACTAAAG AGTTTTTCAG GAGATCTAAA ATTGCAGTGT TTGATAAAAT 
     5401 GTGGACCTAC ATGCGGAGTG CGGAGCCCTC TGTGTTTGTG AGGACTACGG CCGAAGGGGT 
     5461 GGCTAGAGTG CGGAAGTCCA AAGGGAAATA TGCCTACTTG TTGGAGTCCA CGATGAACGA 
     5521 GTACATTGAG CAAAGGAAGC CTTGCGACAC CATGAAAGTT GGTGGAAACC TGGATTCCAA 
     5581 AGGCTATGGC ATCGCAACAC CTAAAGGATC CTCATTAAGA ACCCCAGTAA ATCTTGCAGT 
     5641 ATTGAAACTC AGTGAGCAAG GCGTCTTAGA CAAGCTGAAA AACAAATGGT GGTACGATAA 
     5701 AGGTGAATGT GGAGCCAAGG ACTCTGGAAG TAAGGAAAAG ACCAGTGCCC TCAGTCTGAG 
     5761 CAACGTTGCT GGAGTATTCT ACATCCTTGT CGGGGGCCTT GGTTTGGCAA TGCTGGTGGC 
     5821 TTTGATTGAG TTCTGTTACA AGTCAAGGGC CGAGGCGAAA CGAATGAAGG TGGCAAAGAA 
     5881 TGCACAGAAT ATTAACCCAT CTTCCTCGCA GAATTCACAG AATTTTGCAA CTTATAAGGA 
     5941 AGGTTACAAC GTATATGGCA TCGAAAGTGT TAAAATTACG CGTACGCGGC CGCTCGAGCA 
     6001 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     6061 CGATAAGGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6121 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6181 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6241 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6301 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6361 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6421 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6481 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6541 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     6601 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     6661 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     6721 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     6781 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     6841 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     6901 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     6961 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7021 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7081 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7141 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7201 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7261 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7321 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7381 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7441 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7501 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7561 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     7621 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     7681 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     7741 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     7801 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     7861 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     7921 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     7981 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8041 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8101 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8161 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8221 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8281 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8341 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8401 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8461 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8521 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     8581 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     8641 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     8701 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     8761 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     8821 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     8881 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8941 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT CCCGCGCACA 
     9001 TTTCCCCGAA AAGTGCCACC TGAC
//