LOCUS       RC212098                9048 bp    DNA     circular  SYN 
DEFINITION  DHX57 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57)
ACCESSION   NM_198963
VERSION     NM_198963.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5203..5294 
                     /label="Myc-DDK"  
     misc_feature    5324..5909 
                     /label="PolyA signal"  
     misc_feature    5381..5400 
                     /label="Restriction Enzyme"  
     misc_feature    6185..6857 
                     /label="ColE1"  
     misc_feature    7287..8081 
                     /label="Kan/Neo"  
     misc_feature    8116..8418 
                     /label="SV40 ori"  
     misc_feature    8504..8981 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5178..5185 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5178 
                     /label="DHX57"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTTCA GTAAGAAGAA AAGGCAAGCC AGGCAAAGGA GGTGGAAAAG 
     1081 GGTCTTCTAG AGGAGGAAGA GGAGGCAGGA GTCACGCCAG TAAATCTCAT GGGAGTGGTG 
     1141 GCGGTGGCGG TGGTGGTGGT GGTGGAGGTG GCGGCAACAG AAAGGCCTCC AGTAGAATAT 
     1201 GGGATGATGG AGATGACTTT TGTATCTTCA GTGAATCAAG GCGCCCTTCC AGACCTAGCA 
     1261 ACAGTAACAT AAGCAAAGGA GAGTCACGCC CAAAATGGAA ACCCAAAGCC AAAGTACCCC 
     1321 TTCAGACTCT ACATATGACT TCTGAGAATC AAGAGAAAGT GAAAGCTCTT CTCCGAGACC 
     1381 TGCAAGAACA AGATGCTGAT GCTGGATCTG AAAGAGGCCT TTCTGGGGAG GAGGAAGATG 
     1441 ATGAGCCTGA TTGCTGTAAC GATGAGCGGT ACTGGCCAGC TGGACAGGAA CCTTCCCTCG 
     1501 TTCCCGACTT GGATCCTTTG GAATATGCTG GCTTAGCCTC AGTGGAGCCT TATGTTCCAG 
     1561 AATTTACAGT CTCCCCATTT GCAGTGCAAA AACTTTCCAG GTATGGTTTC AATACTGAAC 
     1621 GCTGTCAAGC GGTCCTGAGG ATGTGTGATG GAGATGTGGG AGCATCACTA GAGCATCTCC 
     1681 TTACCCAGTG TTTTTCAGAG ACATTTGGAG AGAGGATGAA GATCTCTGAG GCAGTCAACC 
     1741 AGATAAGCTT GGATGAGTGT ATGGAACAGC GACAGGAAGA GGCATTTGCT CTCAAGTCCA 
     1801 TCTGTGGAGA AAAATTTATA GAAAGAATTC AGAACAGAGT CTGGACCATT GGGTTAGAAC 
     1861 TGGAGTATCT GACAAGTAGA TTCCGCAAAT CCAAGCCAAA AGAAAGTACC AAAAATGTAC 
     1921 AAGAGAATTC ACTTGAAATC TGTAAATTTT ACCTCAAAGG AAATTGTAAA TTTGGATCAA 
     1981 AATGCAGATT CAAACATGAA GTGCCCCCAA ATCAAATTGT TGGAAGAATA GAAAGAAGTG 
     2041 TAGATGATTC TCATCTTAAT GCTATTGAAG ATGCATCTTT TTTATATGAA CTTGAAATTC 
     2101 GATTTTCTAA AGACCACAAA TATCCCTACC AAGCTCCGCT CGTGGCATTT TATTCCACCA 
     2161 ATGAGAACCT ACCTCTGGCT TGTCGTTTAC ATATTTCTGA GTTTCTTTAT GACAAGGCCT 
     2221 TGACATTTGC GGAAACTTCG GAACCTGTCG TATATTCTTT GATAACCCTT TTAGAGGAAG 
     2281 AGTCGGAAAT AGTCAAGTTA CTAACGAATA CCCACCACAA GTATAGTGAC CCTCCTGTGA 
     2341 ACTTTCTGCC AGTACCCTCT AGGACCAGAA TAAATAATCC TGCCTGTCAT AAAACAGTGA 
     2401 TTCCAAATAA TTCTTTTGTT TCTAATCAAA TTCCAGAAGT TGAAAAAGCA TCAGAATCTG 
     2461 AGGAGTCAGA TGAGGATGAC GGTCCTGCAC CTGTTATAGT AGAGAATGAA AGCTATGTGA 
     2521 ACCTTAAGAA AAAGATTTCC AAAAGATATG ACTGGCAGGC AAAGTCAGTA CATGCTGAAA 
     2581 ATGGTAAAAT CTGCAAGCAG TTCCGAATGA AACAGGCTTC CAGACAGTTC CAGTCCATTC 
     2641 TGCAAGAGAG GCAATCACTC CCTGCTTGGG AAGAAAGAGA AACCATTCTT AACTTATTGC 
     2701 GTAAGCACCA GGTGGTTGTC ATAAGTGGTA TGACTGGATG TGGGAAAACC ACACAAATTC 
     2761 CGCAGTTTAT TCTGGATGAT TCTCTGAGTG GACCACCTGA GAAGGTAGCC AACATCATCT 
     2821 GTACCCAACC CCGACGAATC TCTGCAATCT CTGTTGCTGA ACGCGTTGCT AAAGAAAGAG 
     2881 CAGAGAGGGT GGGTCTGACC GTGGGATACC AGATTCGGTT AGAAAGTGTC AAGTCCTCAG 
     2941 CCACCAGACT GTTATACTGC ACCACGGGAG TGCTGCTGAG AAGGCTAGAA GGAGATACAG 
     3001 CTCTACAAGG AGTTTCCCAT ATCATTGTTG ATGAAGTTCA TGAGAGGACA GAAGAAAGTG 
     3061 ACTTCTTGCT GCTAGTTTTG AAGGACATTG TATCGCAGAG GCCAGGTCTT CAAGTTATTT 
     3121 TAATGAGTGC AACTCTAAAC GCTGAGCTTT TTTCAGACTA TTTTAATTCC TGCCCCGTTA 
     3181 TTACTATACC AGGTCGTACA TTTCCTGTTG ATCAATTTTT TTTGGAAGAT GCAATTGCTG 
     3241 TGACAAGGTA TGTATTACAG GATGGGAGCC CATATATGCG GTCCATGAAA CAGATTTCAA 
     3301 AGGAAAAGCT TAAAGCAAGG CGGAACAGAA CTGCATTTGA AGAAGTGGAA GAAGACCTAA 
     3361 GGCTCTCCCT TCACCTCCAG GATCAGGATT CTGTCAAAGA TGCAGTGCCA GATCAACAGT 
     3421 TAGATTTTAA GCAGCTCCTG GCCCGCTATA AAGGGGTTAG CAAGTCAGTC ATCAAAACAA 
     3481 TGTCCATCAT GGATTTTGAA AAGGTGAATC TTGAATTAAT AGAGGCCTTA TTAGAGTGGA 
     3541 TTGTGGATGG AAAGCACTCC TACCCTCCAG GTGCTATACT TGTATTTTTA CCAGGACTAG 
     3601 CAGAAATCAA AATGCTTTAT GAACAGCTAC AGTCTAATTC TCTTTTCAAC AACAGACGTA 
     3661 GTAATCGATG TGTTATTCAC CCACTTCATT CATCTTTATC CAGTGAAGAG CAGCAGGCTG 
     3721 TGTTTGTAAA ACCTCCTGCA GGAGTAACTA AGATTATAAT TTCCACCAAC ATTGCTGAGA 
     3781 CATCCATAAC CATCGATGAT GTTGTCTATG TTATCGATTC TGGGAAAATG AAAGAAAAGA 
     3841 GATATGATGC CAGCAAAGGG ATGGAAAGTC TAGAGGACAC CTTTGTATCT CAAGCTAATG 
     3901 CTCTACAAAG GAAAGGCCGA GCAGGCCGTG TTGCATCTGG GGTCTGCTTC CATTTATTCA 
     3961 CTAGCCATCA CTACAATCAC CAGCTTTTAA AACAACAGCT ACCAGAAATA CAAAGAGTGC 
     4021 CATTGGAACA GCTGTGTCTA AGAATTAAAA TTTTAGAGAT GTTTAGTGCT CATAATCTCC 
     4081 AGTCTGTGTT CTCTCGGCTC ATTGAACCTC CACACACCGA TTCTCTTCGT GCCTCAAAAA 
     4141 TACGATTACG AGACTTAGGA GCATTAACTC CAGATGAAAG ATTGACCCCT CTTGGGTATC 
     4201 ATTTGGCCTC TCTGCCCGTG GATGTGAGAA TTGGCAAACT AATGTTGTTT GGGTCTATCT 
     4261 TCCGCTGTTT GGATCCTGCT CTCACCATTG CTGCCAGTTT GGCTTTTAAG TCGCCGTTTG 
     4321 TATCTCCCTG GGATAAAAAA GAAGAAGCTA ACCAGAAAAA GCTGGAATTT GCATTCGCAA 
     4381 ACAGTGATTA TCTGGCCCTT CTACAAGCGT ATAAGGGATG GCAGCTAAGT ACAAAAGAAG 
     4441 GCGTGCGTGC AAGTTATAAT TACTGCAGAC AAAACTTCTT GTCTGGAAGA GTTCTGCAGG 
     4501 AAATGGCCAG CCTCAAACGA CAATTCACGG AACTGTTATC GGATATAGGG TTTGCAAGGG 
     4561 AAGGGCTCAG AGCAAGGGAA ATTGAGAAAA GGGCCCAAGG AGGAGATGGT GTCTTAGATG 
     4621 CCACAGGAGA AGAGGCAAAC TCAAATGCTG AGAACCCCAA GCTGATATCA GCAATGCTGT 
     4681 GTGCTGCTTT GTATCCAAAT GTAGTGCAGG TGAAAAGCCC AGAAGGAAAA TTTCAGAAGA 
     4741 CCAGTACTGG AGCTGTCAGA ATGCAACCAA AATCAGCTGA GTTGAAGTTT GTCACCAAGA 
     4801 ACGATGGATA TGTACACATT CACCCTTCAT CAGTGAACTA TCAGGTGAGA CACTTTGACA 
     4861 GCCCCTACCT GTTGTACCAC GAGAAGATAA AAACTAGTCG AGTATTCATC CGAGACTGCA 
     4921 GCATGGTGTC TGTGTACCCG CTGGTCTTGT TTGGAGGAGG CCAAGTGAAT GTGCAGCTTC 
     4981 AAAGAGGAGA GTTCGTTGTC TCCCTGGATG ATGGTTGGAT CCGTTTTGTA GCTGCTTCCC 
     5041 ATCAGGTGGC TGAACTGGTA AAGGAGCTTC GTTGTGAACT TGATCAGCTT CTCCAGGATA 
     5101 AAATTAAAAA CCCAAGCATT GATCTGTGTA CGTGTCCTCG AGGATCCCGG ATCATCAGCA 
     5161 CAATTGTGAA ACTTGTCACC ACACAAAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     5221 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5281 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5341 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5401 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5461 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5521 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5581 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5641 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5701 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5761 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5821 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5881 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5941 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6001 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6061 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6121 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6181 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6241 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6301 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6361 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6421 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6481 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6541 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6601 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6661 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6721 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6781 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6841 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6901 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6961 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7021 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7081 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7141 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7201 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7261 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7321 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7381 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7441 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7501 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7561 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7621 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7681 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7741 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7801 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7861 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7921 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7981 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8041 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8101 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8161 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8221 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8281 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8341 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8401 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8461 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8521 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8581 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8641 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8701 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8761 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8821 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8881 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8941 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9001 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//