LOCUS       RC212045                8814 bp    DNA     circular  SYN 
DEFINITION  AFF2 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 2 (AFF2), transcript variant 1
ACCESSION   NM_002025
VERSION     NM_002025.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4971..5062 
                     /label="Myc-DDK"  
     misc_feature    5092..5677 
                     /label="PolyA signal"  
     misc_feature    5149..5168 
                     /label="Restriction Enzyme"  
     misc_feature    5953..6625 
                     /label="ColE1"  
     misc_feature    7055..7849 
                     /label="Kan/Neo"  
     misc_feature    7884..8186 
                     /label="SV40 ori"  
     misc_feature    8272..8749 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4959 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="AFF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTATTC GACTTTTTCA GAGACTGGGA CTTGGAGCAG CAGTGTCACT 
     1081 ATGAACAAGA CCGTAGTGCA CTTAAAAAAA GGGAATGGGA GCGGAGGAAT CAAGAAGTCC 
     1141 AGCAAGAAGA CGATCTCTTT TCTTCAGGCT TTGATCTTTT TGGGGAGCCA TACAAGGTAG 
     1201 CTGAATATAC AAACAAAGGT GATGCACTTG CCAACCGAGT CCAGAACACG CTTGGAAACT 
     1261 ATGATGAAAT GAAGAATTTG CTAACTAACC ATTCTAATCA GAATCACCTA GTGGGAATTC 
     1321 CAAAGAATTC TGTGCCCCAG AATCCCAACA ACAAAAATGA ACCAAGCTTT TTTCCAGAAC 
     1381 AAAAGAACAG AATAATTCCA CCTCACCAGG ATAATACCCA TCCTTCAGCA CCAATGCCTC 
     1441 CACCTTCTGT TGTGATACTG AATTCAACTC TAATACACAG CAACAGAAAA TCAAAACCTG 
     1501 AGTGGTCACG TGATAGTCAT AACCCTAGCA CTGTACTGGC AAGCCAGGCC AGTGGTCAGC 
     1561 CAAACAAGAT GCAGACTTTG ACACAGGACC AGTCTCAAGC CAAACTGGAA GACTTCTTTG 
     1621 TCTACCCAGC TGAACAGCCC CAGATTGGAG AAGTTGAAGA GTCAAACCCA TCTGCAAAGG 
     1681 AAGACAGTAA CCCTAATTCT AGTGGAGAAG ATGCTTTCAA AGAAATCTTT CAATCCAATT 
     1741 CACCGGAAGA ATCTGAATTC GCCGTGCAAG CGCCTGGGTC TCCCCTAGTG GCTTCCTCTT 
     1801 TATTAGCTCC TAGCAGTGGC CTTTCAGTTC AAAACTTCCC ACCAGGGCTT TACTGCAAAA 
     1861 CAAGCATGGG GCAGCAAAAG CCAACTGCAT ACGTCAGACC CATGGATGGC CAGGACCAGG 
     1921 CACCGGACAT CTCACCAACA CTGAAACCTT CAATTGAATT TGAGAACAGC TTTGGGAATC 
     1981 TGTCATTTGG AACACTCTTG GATGGAAAAC CCAGTGCAGC CAGTTCAAAG ACTAAACTGC 
     2041 CAAAGTTCAC CATCCTCCAA ACAAGTGAAG TAAGCCTTCC CAGTGATCCA AGCTGTGTTG 
     2101 AAGAAATCTT GCGGGAGATG ACCCATTCCT GGCCTACTCC TCTCACTTCC ATGCATACTG 
     2161 CTGGACACTC TGAGCAGAGC ACCTTTTCCA TCCCAGGACA GGAATCGCAG CATCTGACCC 
     2221 CAGGATTCAC CTTACAAAAG TGGAATGACC CAACCACCAG AGCTTCTACA AAGTCAGTGT 
     2281 CTTTCAAATC GATGCTTGAG GATGACCTGA AGCTGAGCAG TGATGAAGAT GACCTTGAGC 
     2341 CTGTGAAGAC CTTGACCACT CAGTGCACTG CCACTGAGCT CTACCAGGCT GTTGAAAAGG 
     2401 CAAAACCTAG GAATAATCCT GTGAACCCAC CCTTGGCCAC TCCCCAGCCC CCACCTGCAG 
     2461 TGCAAGCCAG CGGGGGTTCT GGCAGCTCCA GCGAATCGGA GAGCAGCTCT GAGTCGGATT 
     2521 CAGACACTGA AAGTAGCACC ACTGACAGCG AATCTAATGA GGCACCTCGT GTGGCAACTC 
     2581 CAGAGCCTGA GCCACCCTCA ACCAACAAGT GGCAACTGGA TAAATGGCTT AACAAAGTGA 
     2641 CATCCCAGAA CAAGTCTTTT ATTTGTGGCC CAAATGAAAC ACCCATGGAG ACTATTTCTC 
     2701 TGCCTCCTCC AATCATCCAA CCAATGGAAG TCCAGATGAA AGTGAAGACG AATGCCAGTC 
     2761 AGGTCCCAGC TGAACCCAAA GAAAGGCCTC TCCTCAGTCT CATTAGGGAG AAAGCCCGTC 
     2821 CACGGCCCAC TCAGAAAATT CCAGAAACAA AGGCTTTGAA GCATAAGTTG TCAACAACTA 
     2881 GTGAGACAGT GTCTCAAAGG ACAATTGGGA AAAAACAGCC CAAAAAAGTT GAGAAGAACA 
     2941 CCAGCACTGA CGAGTTTACC TGGCCCAAAC CAAATATTAC CAGCAGCACT CCCAAAGAAA 
     3001 AAGAAAGTGT GGAGCTTCAT GACCCACCAA GAGGCCGCAA CAAAGCCACT GCCCACAAAC 
     3061 CAGCCCCTAG GAAAGAACCA AGACCTAACA TCCCTTTGGC TCCCGAGAAG AAGAAGTACA 
     3121 GAGGGCCTGG CAAGATTGTG CCAAAGTCTC GGGAATTCAT TGAAACAGAT TCATCTACAT 
     3181 CTGACTCCAA CACAGATCAG GAAGAGACCC TGCAAATCAA AGTCCTGCCT CCGTGCATTA 
     3241 TTTCTGGAGG TAATACTGCC AAATCCAAGG AAATCTGTGG TGCCAGCCTG ACCCTCAGCA 
     3301 CCTTAATGAG TAGCAGTGGC AGCAACAACA ACTTATCCAT CAGTAATGAA GAGCCAACAT 
     3361 TTTCACCTAT TCCTGTCATG CAAACTGAAA TCCTGTCCCC TCTGCGAGAT CATGAGAACC 
     3421 TGAAAAACCT CTGGGTGAAG ATTGACCTTG ACTTACTCTC TAGAGTACCT GGCCACAGCT 
     3481 CACTCCATGC AGCACCTGCC AAGCCAGACC ACAAGGAGAC TGCCACAAAA CCCAAGCGTC 
     3541 AGACAGCTGT CACAGCTGTG GAGAAACCAG CCCCTAAGGG CAAACGTAAG CACAAGCCAA 
     3601 TAGAAGTTGC AGAGAAGATC CCTGAGAAGA AGCAGCGCCT GGAGGAGGCC ACAACTATCT 
     3661 GCTTGCTCCC TCCTTGCATC TCACCAGCCC CACCCCACAA GCCTCCCAAC ACTAGAGAAA 
     3721 ATAATTCATC CAGGAGAGCA AATAGAAGAA AGGAAGAAAA ACTATTTCCT CCTCCACTTT 
     3781 CCCCACTGCC AGAGGACCCT CCACGCCGCA GAAATGTCAG TGGCAATAAT GGTCCCTTTG 
     3841 GTCAAGACAA AAACATCGCC ATGACTGGAC AAATCACATC TACCAAACCT AAGAGAACTG 
     3901 AAGGCAAATT CTGTGCTACT TTCAAAGGGA TATCGGTAAA TGAGGGAGAC ACTCCAAAAA 
     3961 AGGCATCCTC TGCCACCATC ACTGTCACCA ATACTGCTAT TGCCACTGCT ACTGTCACTG 
     4021 CTACTGCCAT TGTCACCACC ACTGTCACAG CTACTGCCAC CGCCACGGCC ACCACCACAA 
     4081 CTACTACCAC TACCATTTCC ACCATCACCT CTACCATCAC TACTGGCCTC ATGGATAGCA 
     4141 GTCACCTGGA GATGACGTCC TGGGCGGCTC TGCCCCTTCT ATCCAGCAGC AGCACTAATG 
     4201 TCCGGAGACC CAAGCTCACT TTTGATGACT CGGTTCACAA TGCTGATTAT TACATGCAAG 
     4261 AAGCTAAGAA GCTGAAGCAC AAAGCTGATG CACTGTTCGA GAAATTTGGC AAAGCTGTGA 
     4321 ATTATGCTGA TGCCGCCCTC TCCTTCACTG AATGTGGCAA TGCCATGGAA CGCGACCCTC 
     4381 TGGAAGCAAA GTCCCCATAC ACCATGTACT CTGAGACTGT GGAGCTCCTC AGGTATGCAA 
     4441 TGAGGCTGAA GAACTTTGCA AGTCCCTTGG CTTCGGATGG GGACAAAAAG CTAGCAGTAC 
     4501 TATGCTACCG ATGTTTATCA CTCCTCTATT TGAGAATGTT TAAGCTGAAG AAGGACCATG 
     4561 CTATGAAGTA CTCCAGATCA CTGATGGAAT ATTTTAAGCA AAATGCTTCA AAAGTCGCAC 
     4621 AGATACCCTC TCCATGGGTA AGCAATGGAA AGAACACTCC ATCCCCAGTG TCTCTCAACA 
     4681 ACGTCTCCCC CATCAACGCA ATGGGGAACT GTAACAATGG CCCAGTCACC ATTCCCCAGC 
     4741 GCATTCACCA CATGGCTGCC AGCCACGTCA ACATCACTAG CAATGTGTTA CGGGGCTATG 
     4801 AACACTGGGA TATGGCCGAC AAACTGACAA GAGAAAACAA AGAATTCTTT GGTGATCTGG 
     4861 ACACGCTGAT GGGGCCTCTG ACCCAGCACA GCAGCATGAC CAATCTTGTC CGCTACGTTC 
     4921 GCCAAGGACT GTGTTGGCTG CGCATCGATG CCCACTTGTT GACGCGTACG CGGCCGCTCG 
     4981 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5041 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5101 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5161 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5221 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5281 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5341 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5401 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5461 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5521 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5581 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5641 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5701 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5761 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5821 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5881 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5941 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6001 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6061 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6121 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6181 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6241 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6301 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6361 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6421 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6481 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6541 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6601 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6661 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6721 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6781 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6841 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6901 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6961 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7021 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7081 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7141 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7201 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7261 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7321 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7381 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7441 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7501 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7561 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7621 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7681 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7741 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7801 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7861 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7921 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7981 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8041 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8221 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8281 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8341 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8401 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8461 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8521 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8581 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8641 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8701 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8761 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//