LOCUS       RC211994                9006 bp    DNA     circular  SYN 
DEFINITION  NID2 (Myc-DDK-tagged)-Human nidogen 2 (osteonidogen) (NID2)
ACCESSION   NM_007361
VERSION     NM_007361.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5163..5254 
                     /label="Myc-DDK"  
     misc_feature    5284..5869 
                     /label="PolyA signal"  
     misc_feature    5341..5360 
                     /label="Restriction Enzyme"  
     misc_feature    6145..6817 
                     /label="ColE1"  
     misc_feature    7247..8041 
                     /label="Kan/Neo"  
     misc_feature    8076..8378 
                     /label="SV40 ori"  
     misc_feature    8464..8941 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5145..5151 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5145 
                     /label="NID2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGGGGAC CGGGTGGCCG GGCGGCCGGT GCTGTCGTCG TTACCAGTGC 
     1081 TACTGCTGCT GCAGTTGCTA ATGTTGCGGG CCGCGGCGCT GCACCCAGAC GAGCTCTTCC 
     1141 CACACGGGGA GTCGTGGGGG GACCAGCTCC TGCAGGAAGG CGACGACGAA AGCTCAGCCG 
     1201 TGGTGAAGCT GGCGAATCCC CTGCACTTCT ACGAAGCCCG ATTCAGCAAC CTCTACGTGG 
     1261 GCACCAACGG CATCATCTCC ACTCAGGACT TCCCCAGGGA AACGCAGTAT GTGGACTATG 
     1321 ATTTCCCCAC CGACTTCCCG GCCATCGCCC CTTTTCTGGC GGACATCGAC ACGAGCCACG 
     1381 GCAGAGGCCG AGTCCTGTAC CGAGAGGACA CCTCCCCCGC AGTGCTGGGC CTGGCCGCCC 
     1441 GCTATGTGCG CGCTGGCTTC CCGCGCTCTG CGCGCTTTAC CCCCACCCAC GCCTTCCTGG 
     1501 CCACCTGGGA GCAGGTAGGC GCTTACGAGG AGGTCAAGCG CGGGGCGCTG CCCTCGGGAG 
     1561 AGCTGAACAC TTTCCAGGCA GTTTTGGCAT CTGATGGGTC TGATAGCTAC GCCCTCTTTC 
     1621 TTTATCCTGC CAACGGCCTG CAGTTCCTTG GAACCCGCCC CAAAGAGTCT TACAATGTCC 
     1681 AGCTTCAGCT TCCAGCTCGG GTGGGCTTCT GCCGAGGGGA GGCTGATGAT CTGAAGTCAG 
     1741 AAGGACCATA TTTCAGCTTG ACTAGCACTG AACAGTCTGT GAAAAATCTC TATCAACTAA 
     1801 GCAACCTGGG GATCCCTGGA GTGTGGGCTT TCCATATCGG CAGCACTTCC CCGTTGGACA 
     1861 ATGTCAGGCC AGCTGCAGTT GGAGACCTTT CCGCTGCCCA CTCTTCTGTT CCCCTGGGAC 
     1921 GTTCCTTCAG CCATGCTACA GCCCTGGAAA GTGACTATAA TGAGGACAAT TTGGATTACT 
     1981 ACGATGTGAA TGAGGAGGAA GCTGAATACC TTCCGGGTGA ACCAGAGGAG GCATTGAATG 
     2041 GCCACAGCAG CATTGATGTT TCCTTCCAAT CCAAAGTGGA TACAAAGCCT TTAGAGGAAT 
     2101 CTTCCACCTT GGATCCTCAC ACCAAAGAAG GAACATCTCT GGGAGAGGTA GGGGGCCCAG 
     2161 ATTTAAAAGG CCAAGTTGAG CCCTGGGATG AGAGAGAGAC CAGAAGCCCA GCTCCACCAG 
     2221 AGGTAGACAG AGATTCACTG GCTCCTTCCT GGGAAACCCC ACCACCGTAC CCCGAAAACG 
     2281 GAAGCATCCA GCCCTACCCA GATGGAGGGC CAGTGCCTTC GGAAATGGAT GTTCCCCCAG 
     2341 CTCATCCTGA AGAAGAAATT GTTCTTCGAA GTTACCCTGC TTCAGGTCAC ACTACACCCT 
     2401 TAAGTCGAGG GACGTATGAG GTGGGACTGG AAGACAACAT AGGTTCCAAC ACCGAGGTCT 
     2461 TCACGTATAA TGCTGCCAAC AAGGAAACCT GTGAACACAA CCACAGACAA TGCTCCCGGC 
     2521 ATGCCTTCTG CACGGACTAT GCCACTGGCT TCTGCTGCCA CTGCCAATCC AAGTTTTATG 
     2581 GAAATGGGAA GCACTGTCTG CCTGAGGGGG CACCTCACCG AGTGAATGGG AAAGTGAGTG 
     2641 GCCACCTCCA CGTGGGCCAT ACACCCGTGC ACTTCACTGA TGTGGACCTG CATGCGTATA 
     2701 TCGTGGGCAA TGATGGCAGA GCCTACACGG CCATCAGCCA CATCCCACAG CCAGCAGCCC 
     2761 AGGCCCTCCT CCCCCTCACA CCAATTGGAG GCCTGTTTGG CTGGCTCTTT GCTTTAGAAA 
     2821 AACCTGGCTC TGAGAACGGC TTCAGCCTCG CAGGTGCTGC CTTTACCCAT GACATGGAAG 
     2881 TTACATTCTA CCCGGGAGAG GAGACGGTTC GTATCACTCA AACTGCTGAG GGACTTGACC 
     2941 CAGAGAACTA CCTGAGCATT AAGACCAACA TTCAAGGCCA GGTGCCTTAC GTCCCAGCAA 
     3001 ATTTCACAGC CCACATCTCT CCCTACAAGG AGCTGTACCA CTACTCCGAC TCCACTGTGA 
     3061 CCTCTACAAG TTCCAGAGAC TACTCTCTGA CTTTTGGTGC AATCAACCAA ACATGGTCCT 
     3121 ACCGCATCCA CCAGAACATC ACTTACCAGG TGTGCAGGCA CGCCCCCAGA CACCCGTCCT 
     3181 TCCCCACCAC CCAGCAGCTG AACGTGGACC GGGTCTTTGC CTTGTATAAT GACGAAGAAA 
     3241 GAGTGCTTAG ATTTGCTGTG ACCAATCAAA TTGGCCCGGT CAAAGAAGAT TCAGACCCCA 
     3301 CTCCGGGGAA TCCTTGCTAT GATGGGAGCC ACATGTGTGA CACAACAGCA CGGTGCCATC 
     3361 CAGGGACAGG TGTAGATTAC ACCTGTGAGT GCGCATCTGG GTACCAGGGA GATGGACGGA 
     3421 ACTGTGTGGA TGAAAATGAA TGTGCAACTG GCTTTCATCG CTGTGGCCCC AACTCTGTAT 
     3481 GTATCAACTT GCCTGGAAGC TACAGGTGTG AGTGCCGGAG TGGTTATGAG TTTGCAGATG 
     3541 ACCGGCATAC TTGCATCTTG ATCACCCCAC CTGCCAACCC CTGTGAGGAT GGCAGTCATA 
     3601 CCTGTGCTCC TGCTGGGCAG GCCCGGTGTG TTCACCATGG AGGCAGCACG TTCAGCTGTG 
     3661 CCTGCCTGCC TGGTTATGCC GGCGATGGGC ACCAGTGCAC TGATGTAGAT GAATGCTCAG 
     3721 AAAACAGATG TCACCCTGCA GCTACCTGCT ACAATACTCC TGGTTCCTTC TCCTGCCGTT 
     3781 GTCAACCCGG ATATTATGGG GATGGATTTC AGTGCATACC TGACTCCACC TCAAGCCTGA 
     3841 CACCCTGTGA ACAACAGCAG CGCCATGCCC AGGCCCAGTA TGCCTACCCT GGGGCCCGGT 
     3901 TCCACATCCC CCAATGCGAC GAGCAGGGCA ACTTCCTGCC CCTACAGTGT CATGGCAGCA 
     3961 CTGGTTTCTG CTGGTGCGTG GACCCTGATG GTCATGAAGT TCCTGGTACC CAGACTCCAC 
     4021 CTGGCTCCAC CCCGCCTCAC TGTGGACCAT CACCAGAGCC CACCCAGAGG CCCCCGACCA 
     4081 TCTGTGAGCG CTGGAGGGAA AACCTGCTGG AGCACTACGG TGGCACCCCC CGGGATGACC 
     4141 AGTACGTGCC CCAGTGCGAT GACCTGGGCC ACTTCATCCC CCTGCAGTGC CACGGAAAGA 
     4201 GCGACTTCTG CTGGTGTGTG GACAAAGATG GCAGAGAGGT GCAGGGCACC CGCTCCCAGC 
     4261 CAGGCACCAC CCCTGCGTGT ATACCCACCG TCGCTCCACC CATGGTCCGG CCCACGCCCC 
     4321 GGCCAGATGT GACCCCTCCA TCTGTGGGCA CCTTCCTGCT CTATACTCAG GGCCAGCAGA 
     4381 TTGGCTACTT ACCCCTCAAT GGCACCAGGC TTCAGAAGGA TGCAGCTAAG ACCCTGCTGT 
     4441 CTCTGCATGG CTCCATAATC GTGGGAATTG ATTACGACTG CCGGGAGAGG ATGGTGTACT 
     4501 GGACAGATGT TGCTGGACGG ACAATCAGCC GTGCTGGTCT GGAACTGGGA GCAGAGCCTG 
     4561 AGACGATCGT GAATTCAGGT CTGATAAGCC CTGAAGGACT TGCCATAGAC CACATCCGCA 
     4621 GAACAATGTA CTGGACGGAC AGTGTCCTGG ATAAGATAGA GAGCGCCCTG CTGGATGGCT 
     4681 CTGAGCGCAA GGTCCTCTTC TACACAGATC TGGTGAATCC CCGTGCCATC GCTGTGGATC 
     4741 CAATCCGAGG CAACTTGTAC TGGACAGACT GGAATAGAGA AGCTCCTAAA ATTGAAACGT 
     4801 CATCTTTAGA TGGAGAAAAC AGAAGAATTC TGATCAATAC AGACATTGGA TTGCCCAATG 
     4861 GCTTAACCTT TGACCCTTTC TCTAAACTGC TCTGCTGGGC AGATGCAGGA ACCAAAAAAC 
     4921 TGGAGTGTAC ACTACCTGAT GGAACTGGAC GGCGTGTCAT TCAAAACAAC CTCAAGTACC 
     4981 CCTTCAGCAT CGTAAGCTAT GCAGATCACT TCTACCACAC AGACTGGAGG AGGGATGGTG 
     5041 TTGTATCAGT AAATAAACAT AGTGGCCAGT TTACTGATGA GTATCTCCCA GAACAACGAT 
     5101 CTCACCTCTA CGGGATAACT GCAGTCTACC CCTACTGCCC AACAGGAAGA AAGACGCGTA 
     5161 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5221 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5281 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5341 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5401 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5461 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5521 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5581 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5641 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5701 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5761 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5821 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5881 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5941 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6001 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6061 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6121 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6181 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6241 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6301 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6361 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6421 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6481 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6541 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6601 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6661 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6721 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6781 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6841 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6901 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6961 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7021 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7081 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7141 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7201 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7261 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7321 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7381 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7441 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7501 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7561 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7621 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7681 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7741 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7801 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7861 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7921 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7981 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8041 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8101 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8161 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8221 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8281 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8341 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8401 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8461 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8521 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8581 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8641 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8701 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8761 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8821 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8881 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8941 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9001 AATTTT
//