LOCUS       RC211939                6068 bp    DNA     circular  SYN 
DEFINITION  GJC1 (Myc-DDK-tagged)-Human gap junction protein, gamma 1, 45kDa (GJC1), transcript variant 1
ACCESSION   NM_005497
VERSION     NM_005497.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2225..2316 
                     /label="Myc-DDK"  
     misc_feature    2346..2931 
                     /label="PolyA signal"  
     misc_feature    2403..2422 
                     /label="Restriction Enzyme"  
     misc_feature    3207..3879 
                     /label="ColE1"  
     misc_feature    4309..5103 
                     /label="Kan/Neo"  
     misc_feature    5138..5440 
                     /label="SV40 ori"  
     misc_feature    5526..6003 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2207..2213 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2207 
                     /label="GJC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTGGAGC TTCCTGACTC GCCTGCTAGA GGAGATTCAC AACCATTCCA 
     1081 CATTTGTGGG GAAGATCTGG CTCACTGTTC TGATTGTCTT CCGGATCGTC CTTACAGCTG 
     1141 TAGGAGGAGA ATCCATCTAT TACGATGAGC AAAGCAAATT TGTGTGCAAC ACAGAACAGC 
     1201 CGGGCTGTGA GAATGTCTGT TATGATGCGT TTGCACCTCT CTCCCATGTA CGCTTCTGGG 
     1261 TGTTCCAGAT CATCCTGGTG GCAACTCCCT CTGTGATGTA CCTGGGCTAT GCTATCCACA 
     1321 AGATTGCCAA AATGGAGCAC GGTGAAGCAG ACAAGAAGGC AGCTCGGAGC AAGCCCTATG 
     1381 CAATGCGCTG GAAACAACAC CGGGCTCTGG AAGAAACGGA GGAGGACAAC GAAGAGGATC 
     1441 CTATGATGTA TCCAGAGATG GAGTTAGAAA GTGATAAGGA AAATAAAGAG CAGAGCCAAC 
     1501 CCAAACCTAA GCATGATGGC CGACGACGGA TTCGGGAAGA TGGGCTCATG AAAATCTATG 
     1561 TGCTGCAGTT GCTGGCAAGG ACCGTGTTTG AGGTGGGTTT TCTGATAGGG CAGTATTTTC 
     1621 TGTATGGCTT CCAAGTCCAC CCGTTTTATG TGTGCAGCAG ACTTCCTTGT CCTCATAAGA 
     1681 TAGACTGCTT TATTTCTAGA CCCACTGAAA AGACCATCTT CCTTCTGATA ATGTATGGTG 
     1741 TTACAGGCCT TTGCCTCTTG CTTAACATTT GGGAGATGCT TCATTTAGGG TTTGGGACCA 
     1801 TTCGAGACTC ACTAAACAGT AAAAGGAGGG AACTTGAGGA TCCGGGTGCT TATAATTATC 
     1861 CTTTCACTTG GAATACACCA TCTGCTCCCC CTGGCTATAA CATTGCTGTC AAACCAGATC 
     1921 AAATCCAGTA CACCGAACTG TCCAATGCTA AGATCGCCTA CAAGCAAAAC AAGGCCAACA 
     1981 CAGCCCAGGA ACAGCAGTAT GGCAGCCATG AGGAGAACCT CCCAGCTGAC CTGGAGGCTC 
     2041 TGCAGCGGGA GATCAGGATG GCTCAGGAAC GCTTGGATCT GGCAGTTCAG GCCTACAGTC 
     2101 ACCAAAACAA CCCTCATGGT CCCCGGGAGA AGAAGGCCAA AGTGGGGTCC AAAGCTGGGT 
     2161 CCAACAAAAG CACTGCCAGT AGCAAATCAG GGGATGGGAA GACCTCCGCT GGATTACGCG 
     2221 TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT 
     2281 GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT 
     2341 GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG 
     2401 TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG 
     2461 ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG 
     2521 TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG 
     2581 CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC 
     2641 CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT 
     2701 GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA 
     2761 TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC 
     2821 CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC 
     2881 TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT 
     2941 GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA 
     3001 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG 
     3061 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 
     3121 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG 
     3181 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 
     3241 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA 
     3301 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC 
     3361 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT 
     3421 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT 
     3481 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 
     3541 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT 
     3601 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT 
     3661 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT 
     3721 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 
     3781 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 
     3841 AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG 
     3901 TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA 
     3961 ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG 
     4021 GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT 
     4081 ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC 
     4141 CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG 
     4201 GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA 
     4261 TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC 
     4321 AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC 
     4381 CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG 
     4441 TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC 
     4501 CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA 
     4561 CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA 
     4621 GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC 
     4681 GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG 
     4741 TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG 
     4801 ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG 
     4861 TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG 
     4921 CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG 
     4981 GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG 
     5041 CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT 
     5101 CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT 
     5161 AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG 
     5221 GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC 
     5281 GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA 
     5341 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT 
     5401 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA 
     5461 CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC 
     5521 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     5581 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     5641 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     5701 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     5761 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     5821 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     5881 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     5941 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     6001 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     6061 CGAATTTT
//