LOCUS       RC211873                7785 bp    DNA     circular  SYN 
DEFINITION  SLC8A1 (Myc-DDK-tagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 1 (SLC8A1), transcript variant A
ACCESSION   NM_021097
VERSION     NM_021097.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3942..4033 
                     /label="Myc-DDK"  
     misc_feature    4063..4648 
                     /label="PolyA signal"  
     misc_feature    4120..4139 
                     /label="Restriction Enzyme"  
     misc_feature    4924..5596 
                     /label="ColE1"  
     misc_feature    6026..6820 
                     /label="Kan/Neo"  
     misc_feature    6855..7157 
                     /label="SV40 ori"  
     misc_feature    7243..7720 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3924..3930 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3924 
                     /label="SLC8A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACAACATG CGGCGATTAA GTCTTTCACC CACCTTTTCA ATGGGATTTC 
     1081 ATCTGTTAGT TACTGTGAGT CTCTTATTTT CCCATGTGGA CCATGTAATT GCTGAGACAG 
     1141 AAATGGAAGG AGAAGGAAAT GAAACTGGTG AATGTACTGG ATCATATTAC TGTAAGAAAG 
     1201 GGGTGATTTT GCCCATTTGG GAACCCCAAG ACCCTTCTTT TGGGGACAAA ATTGCTAGAG 
     1261 CTACTGTGTA TTTTGTGGCC ATGGTCTACA TGTTTCTTGG AGTCTCTATC ATAGCTGATC 
     1321 GGTTCATGTC CTCTATAGAA GTCATCACAT CTCAAGAAAA AGAAATAACC ATAAAGAAAC 
     1381 CCAATGGAGA GACCACCAAG ACAACTGTGA GGATCTGGAA TGAAACAGTT TCTAACCTGA 
     1441 CCTTGATGGC CCTGGGATCT TCTGCTCCTG AGATTCTCCT TTCAGTAATT GAAGTGTGTG 
     1501 GCCATAACTT CACTGCAGGA GACCTCGGTC CTAGCACCAT CGTGGGAAGT GCTGCATTCA 
     1561 ATATGTTCAT CATTATTGCA CTCTGTGTTT ATGTGGTGCC TGACGGAGAG ACAAGGAAGA 
     1621 TTAAGCATTT GCGTGTCTTC TTTGTGACAG CAGCCTGGAG CATCTTTGCC TACACCTGGC 
     1681 TTTACATTAT TTTGTCTGTC ATATCTCCTG GTGTTGTGGA GGTCTGGGAA GGTTTGCTTA 
     1741 CTTTCTTCTT CTTTCCCATC TGTGTTGTGT TCGCTTGGGT AGCGGATAGG AGACTTCTGT 
     1801 TTTACAAGTA TGTCTACAAG AGGTATCGAG CTGGCAAGCA GAGGGGGATG ATTATTGAAC 
     1861 ATGAAGGAGA CAGGCCATCT TCTAAGACTG AAATTGAAAT GGACGGGAAA GTGGTCAATT 
     1921 CTCATGTTGA AAATTTCTTA GATGGTGCTC TGGTTCTGGA GGTGGATGAG AGGGACCAAG 
     1981 ATGATGAAGA AGCTAGGCGA GAAATGGCTA GGATTCTGAA GGAACTTAAG CAGAAGCATC 
     2041 CAGATAAAGA AATAGAGCAA TTAATAGAAT TAGCTAACTA CCAAGTCCTA AGTCAGCAGC 
     2101 AAAAAAGTAG AGCATTTTAT CGCATTCAAG CTACTCGCCT CATGACTGGA GCTGGCAACA 
     2161 TTTTAAAGAG GCATGCAGCT GACCAAGCAA GGAAGGCTGT CAGCATGCAC GAGGTCAACA 
     2221 CTGAAGTGAC TGAAAATGAC CCTGTTAGTA AGATCTTCTT TGAACAAGGG ACATATCAGT 
     2281 GTCTGGAGAA CTGTGGTACT GTGGCCCTTA CCATTATCCG CAGAGGTGGT GATTTGACTA 
     2341 ACACTGTGTT TGTTGACTTC AGAACAGAGG ATGGCACAGC AAATGCTGGG TCTGATTATG 
     2401 AATTTACTGA AGGAACTGTG GTGTTTAAGC CTGGTGATAC CCAGAAGGAA ATCAGAGTGG 
     2461 GTATCATAGA TGATGATATC TTTGAGGAGG ATGAAAATTT CCTTGTGCAT CTCAGCAATG 
     2521 TCAAAGTATC TTCTGAAGCT TCAGAAGATG GCATACTGGA AGCCAATCAT GTTTCTACAC 
     2581 TTGCTTGCCT CGGATCTCCC TCCACTGCCA CTGTAACTAT TTTTGATGAT GACCACGCAG 
     2641 GCATTTTTAC TTTTGAGGAA CCTGTGACTC ATGTGAGTGA GAGCATTGGC ATCATGGAGG 
     2701 TGAAAGTATT GAGAACATCT GGAGCTCGAG GAAATGTTAT CGTTCCATAT AAAACCATCG 
     2761 AAGGGACTGC CAGAGGTGGA GGGGAGGATT TTGAGGACAC TTGTGGAGAG CTCGAATTCC 
     2821 AGAATGATGA AATTGTCAAA ACAATATCAG TCAAGGTAAT TGATGATGAG GAGTATGAGA 
     2881 AAAACAAGAC CTTCTTCCTT GAGATTGGAG AGCCCCGCCT GGTGGAGATG AGTGAGAAGA 
     2941 AAGCCCTGTT ATTGAATGAG CTTGGTGGCT TCACAATAAC AGGCCAACCT GTCTTCAGGA 
     3001 AGGTTCATGC TAGAGAACAT CCGATTCTCT CTACTGTAAT CACCATTGCA GACGAATATG 
     3061 ATGACAAGCA GCCACTGACC AGCAAAGAGG AAGAGGAGAG GCGCATTGCA GAAATGGGGC 
     3121 GCCCCATCCT GGGAGAGCAC ACCAAGTTGG AAGTGATCAT TGAAGAATCC TATGAATTCA 
     3181 AGAGTACTGT GGACAAACTC ATTAAGAAGA CAAACCTGGC CCTTGTGGTT GGGACTAACA 
     3241 GCTGGAGAGA ACAGTTCATT GAAGCTATCA CTGTCAGTGC TGGGGAAGAT GATGACGACG 
     3301 ATGAATGTGG GGAAGAGAAG CTGCCCTCCT GTTTCGATTA CGTGATGCAC TTTCTGACTG 
     3361 TGTTCTGGAA GGTCCTGTTT GCCTTCGTCC CCCCTACTGA ATACTGGAAT GGCTGGGCGT 
     3421 GTTTCATTGT CTCCATCCTC ATGATTGGCC TACTGACAGC TTTCATTGGA GACCTGGCTT 
     3481 CCCACTTTGG CTGCACCATT GGCCTGAAAG ATTCTGTGAC TGCAGTCGTG TTCGTCGCAC 
     3541 TTGGAACATC AGTGCCAGAC ACATTTGCCA GCAAAGTGGC AGCCACCCAG GACCAGTATG 
     3601 CAGACGCCTC CATAGGTAAC GTCACGGGCA GCAACGCGGT GAATGTCTTC CTGGGAATCG 
     3661 GTGTGGCCTG GTCCATCGCT GCCATCTACC ACGCAGCCAA TGGGGAACAG TTCAAAGTGT 
     3721 CCCCTGGCAC ACTAGCTTTC TCTGTCACTC TCTTCACCAT TTTTGCTTTC ATCAATGTGG 
     3781 GGGTGCTGCT GTATCGGCGG AGGCCAGAAA TCGGAGGTGA GCTGGGTGGG CCCCGGACTG 
     3841 CCAAGCTCCT CACATCCTGC CTCTTTGTGC TCCTATGGCT CTTGTACATT TTCTTCTCCT 
     3901 CCCTGGAGGC CTACTGCCAC ATAAAAGGCT TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3961 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4021 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4081 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4141 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4201 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4261 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4321 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4381 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4441 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4501 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4561 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4621 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4681 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4741 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4801 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4861 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4921 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4981 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5041 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5101 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5161 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5221 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5281 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5341 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5401 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5461 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5521 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5581 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5641 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5701 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5761 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5821 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5881 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5941 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6001 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6061 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6121 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6181 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6241 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6301 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6361 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6421 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6481 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6541 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6601 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6661 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6721 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6781 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6841 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6901 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6961 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7021 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7141 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7201 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7261 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7321 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7381 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7441 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7501 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7561 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7621 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7681 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7741 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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