LOCUS       RC211865                8052 bp    DNA     circular  SYN 
DEFINITION  HERC4 (Myc-DDK-tagged)-Human hect domain and RLD 4 (HERC4), transcript variant 1
ACCESSION   NM_022079
VERSION     NM_022079.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4209..4300 
                     /label="Myc-DDK"  
     misc_feature    4330..4915 
                     /label="PolyA signal"  
     misc_feature    4387..4406 
                     /label="Restriction Enzyme"  
     misc_feature    5191..5863 
                     /label="ColE1"  
     misc_feature    6293..7087 
                     /label="Kan/Neo"  
     misc_feature    7122..7424 
                     /label="SV40 ori"  
     misc_feature    7510..7987 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4191..4197 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4191 
                     /label="HERC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTGCTGG GGAAATGCAT CCTTTGGGCA GCTAGGTTTG GGTGGAATTG 
     1081 ATGAAGAAAT TGTACTAGAG CCCAGAAAAA GTGACTTCTT TATAAATAAA AGGGTCCGAG 
     1141 ATGTAGGATG TGGACTCAGA CATACTGTGT TTGTTCTGGA TGATGGAACA GTGTACACAT 
     1201 GTGGATGTAA TGATCTAGGA CAGCTAGGTC ATGAAAAATC CAGAAAGAAA CCAGAGCAGG 
     1261 TTGTTGCCCT GGATGCCCAA AATATTGTAG CTGTTTCATG TGGAGAAGCT CATACGTTAG 
     1321 CGCTAAATGA CAAAGGCCAG GTGTATGCTT GGGGTCTCGA TTCTGATGGA CAGCTTGGCC 
     1381 TGGTAGGATC AGAGGAATGC ATCAGAGTAC CCAGAAATAT TAAAAGTTTG TCAGATATCC 
     1441 AGATTGTACA GGTTGCTTGT GGTTACTATC ATTCACTTGC ACTTTCTAAA GCAAGTGAAG 
     1501 TCTTCTGTTG GGGACAGAAT AAATATGGCC AATTGGGTTT AGGTACTGAC TGTAAAAAGC 
     1561 AAACTTCACC GCAGCTGCTT AAGTCTTTGC TTGGAATCCC TTTCATGCAA GTTGCAGCAG 
     1621 GAGGAGCCCA TAGTTTTGTA CTCACCCTTT CTGGAGCTAT CTTTGGATGG GGACGCAACA 
     1681 AGTTTGGTCA GCTAGGTCTT AATGATGAAA ATGATAGGTA TGTTCCTAAT TTACTAAAGT 
     1741 CACTAAGATC TCAGAAAATA GTTTATATTT GTTGTGGAGA AGATCATACT GCTGCTCTAA 
     1801 CCAAGGAAGG TGGAGTGTTT ACTTTTGGAG CTGGAGGGTA TGGTCAGTTG GGCCATAATT 
     1861 CTACCAGTCA TGAAATAAAC CCAAGGAAAG TTTTTGAACT TATGGGAAGC ATTGTCACTG 
     1921 AGATTGCTTG TGGACGGCAG CACACTTCTG CTTTTGTTCC TTCATCAGGA CGAATTTACT 
     1981 CTTTTGGGCT TGGTGGTAAT GGGCAGCTGG GAACCGGTTC AACAAGCAAC AGGAAAAGCC 
     2041 CCTTTACTGT AAAAGGAAAT TGGTACCCCT ATAATGGGCA GTGTCTACCA GATATTGATT 
     2101 CTGAAGAATA TTTCTGTGTA AAAAGAATTT TCTCAGGGGG AGATCAAAGC TTTTCACATT 
     2161 ACTCTAGTCC CCAGAACTGT GGGCCACCAG ATGACTTCAG ATGTCCCAAT CCGACAAAGC 
     2221 AGATCTGGAC AGTGAATGAA GCTCTAATTC AGAAATGGCT GAGCTATCCT TCTGGAAGGT 
     2281 TTCCTGTGGA GATAGCCAAT GAGATAGATG GAACGTTTTC TTCCTCTGGT TGCCTAAATG 
     2341 GAAGTTTTTT AGCTGTTAGC AATGATGATC ACTATAGAAC AGGTACCAGA TTTTCAGGGG 
     2401 TTGATATGAA TGCTGCTAGG CTTTTATTCC ACAAACTTAT ACAACCTGAT CATCCGCAGA 
     2461 TATCTCAGCA GGTGGCAGCT AGTTTGGAAA AGAATCTTAT TCCTAAACTG ACTAGCTCCT 
     2521 TACCTGATGT TGAAGCATTG AGGTTTTATC TTACTCTACC AGAATGTCCC CTGATGAGTG 
     2581 ATTCCAACAA TTTCACAACA ATAGCAATTC CCTTTGGTAC AGCTCTTGTG AACCTAGAAA 
     2641 AGGCACCACT GAAAGTACTT GAAAACTGGT GGTCAGTACT TGAACCTCCA CTATTCCTCA 
     2701 AGATAGTAGA ACTTTTTAAG GAAGTTGTGG TACATCTTTT GAAACTCTAC AAGATCGGTA 
     2761 TTCCCCCTTC TGAAAGAAGA ATTTTCAACA GTTTTCTTCA TACTGCATTA AAGGTTTTAG 
     2821 AAATACTACA TAGGGTAAAT GAGAAAATGG GACAGATTAT ACAGTATGAT AAATTTTATA 
     2881 TACATGAAGT ACAAGAATTG ATAGACATAA GAAATGATTA TATCAACTGG GTCCAACAGC 
     2941 AGGCCTATGG AATGGATGTC AACCATGGAT TAACTGAGTT GGCAGATATC CCTGTTACAA 
     3001 TCTGTACATA TCCATTTGTA TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAGACCGATG 
     3061 CAGTCTTACA GATGCAGATG GCTATTGATC AGGCCCACAG GCAGAATGTC TCCTCTCTTT 
     3121 TTCTCCCAGT GATTGAATCT GTGAATCCCT GCTTAATTCT AGTGGTGCGT AGAGAAAATA 
     3181 TTGTAGGAGA TGCAATGGAA GTCCTTAGGA AAACAAAGAA CATAGATTAC AAGAAGCCAC 
     3241 TCAAGGTTAT ATTTGTTGGA GAAGATGCTG TGGATGCAGG AGGGGTGCGC AAAGAATTTT 
     3301 TCTTGCTCAT CATGAGGGAA TTATTGGATC CTAAATACGG CATGTTTAGG TATTATGAAG 
     3361 ATTCCAGGCT CATTTGGTTT TCTGATAAGA CATTTGAAGA CAGTGATTTG TTCCATTTGA 
     3421 TTGGTGTTAT CTGTGGCTTA GCAATTTATA ATTGTACCAT TGTGGACCTC CATTTTCCTT 
     3481 TGGCTTTATA TAAGAAACTA CTGAAAAAGA AGCCATCCTT GGATGATTTG AAAGAACTAA 
     3541 TGCCTGATGT TGGGAGAAGC ATGCAACAGT TACTGGATTA TCCAGAAGAT GACATAGAGG 
     3601 AAACATTTTG TCTTAATTTT ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG 
     3661 AGCTGGTTCT AAATGGTGCA GACACAGCTG TTAACAAACA AAATCGGCAA GAGTTTGTCG 
     3721 ATGCTTATGT GGATTACATA TTCAATAAAT CAGTGGCTTC CTTATTTGAT GCTTTTCATG 
     3781 CGGGCTTTCA TAAGGTCTGT GGAGGAAAAG TCCTTCTGCT CTTTCAGCCT AATGAACTAC 
     3841 AAGCAATGGT CATTGGAAAT ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT 
     3901 ACAAAGGGGA ATATTGGGCA GAACATCCTA CGATAAAAAT TTTTTGGGAA GTATTTCACG 
     3961 AATTACCATT GGAAAAGAAG AAACAGTTTC TGTTATTTTT GACAGGTAGT GATCGCATTC 
     4021 CTATTCTTGG TATGAAGAGT CTGAAACTAG TCATCCAGTC CACAGGAGGT GGTGAGGAGT 
     4081 ATCTCCCAGT TTCCCATACT TGTTTTAATC TTCTGGATCT TCCAAAATAT ACAGAAAAAG 
     4141 AAACTCTACG CTCTAAACTG ATCCAAGCTA TTGATCACAA TGAAGGCTTC AGTTTAATAA 
     4201 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4261 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4321 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4381 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4441 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4501 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4561 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4621 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4681 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4741 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4801 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4861 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4921 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4981 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5041 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5101 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5161 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5221 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5281 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5341 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5401 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5461 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5521 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5581 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5641 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5701 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5761 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5821 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5881 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5941 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6001 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6061 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6121 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6181 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6241 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6301 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6361 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6421 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6481 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6541 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6601 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6661 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6721 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6781 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6841 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6901 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6961 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7021 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7081 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7141 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7201 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7261 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7321 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7381 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7441 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7501 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7561 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7621 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7681 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7741 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7801 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7861 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7921 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7981 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8041 AACGCGAATT TT
//