LOCUS       RC211823                8625 bp    DNA     circular  SYN 
DEFINITION  ANKS1B (Myc-DDK-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 1B (ANKS1B), transcript variant 1
ACCESSION   NM_152788
VERSION     NM_152788.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="ANKS1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGGAC CAGGAGCTGC TGGAAGCTGC TCGCACTGGA AATGTGGCTC 
     1081 TGGTGGAGAA ACTCCTGTCT GGCAGGAAAG GAGGGATCCT GGGCGGTGGA TCCGGACCCC 
     1141 TGCCCCTGTC TAATCTGCTA AGCATCTGGC GAGGCCCCAA TGTGAACTGC ACAGACAGTT 
     1201 CGGGTTACAC TGCTTTACAC CACGCAGCCT TAAATGGACA TAAGGACATA GTTCTCAAAC 
     1261 TACTTCAGTA TGAGGCATCA ACAAATGTAG CAGACAACAA AGGGTATTTT CCTATTCACC 
     1321 TGGCTGCCTG GAAAGGAGAT GTGGAAATTG TGAAGATTCT TATTCATCAT GGACCATCAC 
     1381 ATTCCAGGGT CAATGAACAG AACAATGAAA ATGAAACTGC CCTACACTGT GCAGCTCAAT 
     1441 ATGGACACTC AGAAGTAGTT GCTGTTCTCC TAGAAGAGCT CACTGACCCG ACAATTAGAA 
     1501 ATAGCAAGCT GGAAACACCT TTGGACTTGG CGGCACTCTA CGGACGGCTT AGAGTGGTAA 
     1561 AAATGATCAT CAGTGCACAT CCTAACTTAA TGAGCTGCAA CACTCGCAAG CACACGCCAC 
     1621 TTCACCTTGC TGCGCGCAAT GGCCACAAAG CAGTCGTGCA GGTGCTGCTG GAGGCAGGAA 
     1681 TGGATGTGAG CTGTCAAACA GAAAAGGGGA GTGCACTTCA TGAAGCAGCT TTGTTTGGAA 
     1741 AGGTGGATGT TGTACGAGTT CTGTTAGAAA CAGGAATTGA TGCCAACATA AAGGATAGCT 
     1801 TAGGTAGAAC TGTCTTGGAC ATTCTGAAAG AACATCCATC TCAGAAATCT CTCCAGATTG 
     1861 CAACACTCTT ACAAGAGTAT TTAGAAGGCG TGGGAAGATC TACAGTCCTC GAAGAGCCTG 
     1921 TACAGGAAGA TGCAACACAA GAAACACACA TTTCATCTCC TGTTGAGTCT CCTTCCCAAA 
     1981 AGACCAAAAG TGAAACCGTC ACTGGAGAAT TATCAAAACT CTTGGATGAA ATAAAACTCT 
     2041 GTCAAGAAAA GGATTATTCG TTTGAAGACT TGTGCCACAC AATATCAGAC CACTACTTAG 
     2101 ATAATTTGAG CAAGATTTCA GAGGAAGAAC TTGGGAAAAA TGGAAGCCAG AGTGTAAGAA 
     2161 CCTCATCTAC AATCAATTTG TCACCAGGAG AAGTGGAAGA AGAGGATGAT GATGAAAATA 
     2221 CGTGTGGGCC ATCAGGACTT TGGGAAGCAT TAACTCCGTG TAATGGATGT AGGAACCTTG 
     2281 GCTTCCCCAT GCTTGCCCAG GAGTCCTATC CAAAGAAGAG AAATTACACT ATGGAAATTG 
     2341 TACCATCTGC TTCTCTGGAT ACATTTCCTT CAGAAAATGA GAACTTTCTG TGTGATCTCA 
     2401 TGGACACAGC TGTTACAAAG AAACCTTGCT CCTTAGAAAT TGCAAGGGCA CCTTCCCCAA 
     2461 GAACTGATAA TGCCTCTGAG GTAGCAGTTA CTACTCCAGG AACTAGTAAC CATAGAAACA 
     2521 GCTCAACAGG CCCAACACCT GATTGTTCAC CTCCATCCCC TGATACTGCC CTCAAAAATA 
     2581 TTGTAAAAGT CATTCGACCC CAGCCTAAAC AGCGAACATC CATTGTGTCT TCTCTGGATT 
     2641 TTCACCGAAT GAATCACAAC CAAGAATATT TTGAAATCAA CACATCTACA GGGTGCACAA 
     2701 GCTTTACTGC CAGTCCTCCT GCTAGTCCAC CCACCTCTTC TGTGGGAACC ACAGAAGTCA 
     2761 AGAATGAGGG AACTAACCAT ACAGATGACC TCTCCCGACA GGATGACAAT GATCCCCCAA 
     2821 AAGAATATGA TCCTGGGCAA TTTGCAGGCC TGCTCCATGG ATCCTCTCCA GCCTGTGAGT 
     2881 CCCCTGAAAA TCCATTTCAT CTCTATGGGA AAAGAGAACA ATGTGAAAAA GGACAAGATG 
     2941 AAGTCAGTTT GGCAAACAGT CCTTTGCCTT TCAAGCAGTC TCCAATAGAA AATAACTCAG 
     3001 AACCTTTGGT AAAGAAAATT AAACCCAAAG TGGTCAGTAG AACAATTTTT CACAAAAAAA 
     3061 GCAACCAACT CGAAAACCAT ACCATTGTTG GCACAAGATC AACCAGGAGT GGATCTCGGA 
     3121 ATGGGGACCA GTGGGTTATG AACGCAGGGG GATTTGTGGA GAGAGCCTGT ACTCTGGGGA 
     3181 GAATAAGGTC ATTGCCTAAA GCCTTGATCG ACATGCATTT GTCAAAAAGT GTCTCTAAAT 
     3241 CTGATTCTGA TCTCATTGCC TATCCTTCCA ATGAGAAAAC ATCAAGAGTT AACTGGAGTG 
     3301 AATCTTCCAC TGCTGAACAC AGTTCTAAAG GGAATTCTGA AAGAACACCA TCCTTCACAT 
     3361 CGGAATGGGA AGAAATTGAC AAAATAATGA GTTCCATAGA TGTTGGAATC AACAACGAAC 
     3421 TTAAAGAGAT GAATGGTGAG ACCACAAGAC CCAGATGCCC TGTCCAAACA GTGGGACAAT 
     3481 GGTTGGAAAG CATTGGGCTA CCTCAGTACG AGAACCACCT GATGGCTAAT GGATTTGACA 
     3541 ATGTGCAGTT TATGGGAAGC AATGTTATGG AAGATCAGGA TTTGTTGGAA ATTGGAATCC 
     3601 TTAATTCTGG GCACAGACAA AGAATTCTAC AGGCAATCCA GCTCCTTCCA AAGATGAGAC 
     3661 CCATTGGGCA TGATGGCTAC CATCCCACCT CTGTAGCTGA GTGGCTGGAT TCCATTGAAC 
     3721 TGGGCGACTA CACCAAAGCC TTTCTAATTA ATGGCTACAC TTCGATGGAC CTGTTGAAAA 
     3781 AAATCTGGGA GGTTGAACTT ATTAATGTTT TAAAAATCAA TTTGATTGGC CACAGGAAAC 
     3841 GTATTTTGGC ATCTCTGGGA GACAGGCTGC ACGACGATCC CCCACAGAAG CCCCCTCGGT 
     3901 CCATCACCCT CAGGGAACCC AGTGGTAATC ACACTCCTCC TCAGTTGTCT CCATCACTTA 
     3961 GCCAAAGCAC TTACACCACT GGTGGCTCCC TAGACGTTCC TCACATTATC ATGCAGGGCG 
     4021 ATGCAAGGAG GAGAAGAAAT GAAAACTACT TTGATGATAT TCCCCGATCA AAACTGGAGA 
     4081 GGCAGATGGC TCAGACAGGA GACTGGGGAG AACCTTCCAT TACCTTGCGA CCTCCGAATG 
     4141 AAGCCACAGC CTCTACCCCG GTACAGTACT GGCAGCATCA CCCAGAAAAG CTTATCTTCC 
     4201 AGTCGTGTGA TTACAAAGCT TTTTATTTAG GTTCTATGCT GATAAAAGAG CTTAGGGGGA 
     4261 CAGAATCAAC CCAAGATGCT TGTGCAAAAA TGCGGGCTAA CTGTCAGAAG TCTACAGAGC 
     4321 AAATGAAGAA GGTCCCTACT ATTATTCTTT CTGTCTCATA TAAAGGAGTC AAATTTATTG 
     4381 ATGCAACAAA TAAGAACATA ATTGCTGAGC ATGAAATTCG TAATATCTCC TGTGCTGCCC 
     4441 AGGACCCAGA AGACCTCTCA ACATTTGCCT ATATCACAAA AGATTTGAAG TCTAATCACC 
     4501 ACTACTGTCA TGTGTTTACT GCCTTTGATG TGAATTTAGC CTATGAAATC ATCCTAACCC 
     4561 TGGGACAGGC ATTCGAAGTC GCTTACCAGC TAGCACTACA AGCAAGAAAA GGGGGACACT 
     4621 CCTCCACACT TCCAGAAAGC TTTGAAAACA AACCCTCCAA ACCCATCCCC AAGCCCCGCG 
     4681 TTAGCATTCG CAAGTCCGTG GATCTTCTTC ATGCCAGCCA TACCGGGCAG GAACCATCAG 
     4741 AGAGACACAC TGAGGAGGCA CTCAGAAAAT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//