LOCUS RC211766 9279 bp DNA circular SYN DEFINITION COL3A1 (Myc-DDK-tagged)-Human collagen, type III, alpha 1 (COL3A1) ACCESSION NM_000090 VERSION NM_000090.2 SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 5436..5527 /label="Myc-DDK" misc_feature 5557..6142 /label="PolyA signal" misc_feature 5614..5633 /label="Restriction Enzyme" misc_feature 6418..7090 /label="ColE1" misc_feature 7520..8314 /label="Kan/Neo" misc_feature 8349..8651 /label="SV40 ori" misc_feature 8737..9214 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 5418..5424 /label="Restriction Enzyme" misc_feature 1020..5418 /label="COL3A1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GATGAGCTTT GTGCAAAAGG GGAGCTGGCT ACTTCTCGCT CTGCTTCATC 1081 CCACTATTAT TTTGGCACAA CAGGAAGCTG TTGAAGGAGG ATGTTCCCAT CTTGGTCAGT 1141 CCTATGCGGA TAGAGATGTC TGGAAGCCAG AACCATGCCA AATATGTGTC TGTGACTCAG 1201 GATCCGTTCT CTGCGATGAC ATAATATGTG ACGATCAAGA ATTAGACTGC CCCAACCCAG 1261 AAATTCCATT TGGAGAATGT TGTGCAGTTT GCCCACAGCC TCCAACTGCT CCTACTCGCC 1321 CTCCTAATGG TCAAGGACCT CAAGGCCCCA AGGGAGATCC AGGCCCTCCT GGTATTCCTG 1381 GGAGAAATGG TGACCCTGGT ATTCCAGGAC AACCAGGGTC CCCTGGTTCT CCTGGCCCCC 1441 CTGGAATCTG TGAATCATGC CCTACTGGTC CTCAGAACTA TTCTCCCCAG TATGATTCAT 1501 ATGATGTCAA GTCTGGAGTA GCAGTAGGAG GACTCGCAGG CTATCCTGGA CCAGCTGGCC 1561 CCCCAGGCCC TCCCGGTCCC CCTGGTACAT CTGGTCATCC TGGTTCCCCT GGATCTCCAG 1621 GATACCAAGG ACCCCCTGGT GAACCTGGGC AAGCTGGTCC TTCAGGCCCT CCAGGACCTC 1681 CTGGTGCTAT AGGTCCATCT GGTCCTGCTG GAAAAGATGG AGAATCAGGT AGACCCGGAC 1741 GACCTGGAGA GCGAGGATTG CCTGGACCTC CAGGTATCAA AGGTCCAGCT GGGATACCTG 1801 GATTCCCTGG TATGAAAGGA CACAGAGGCT TCGATGGACG AAATGGAGAA AAGGGTGAAA 1861 CAGGTGCTCC TGGATTAAAG GGTGAAAATG GTCTTCCAGG CGAAAATGGA GCTCCTGGAC 1921 CCATGGGTCC AAGAGGGGCT CCTGGTGAGC GAGGACGGCC AGGACTTCCT GGGGCTGCAG 1981 GTGCTCGGGG TAATGACGGT GCTCGAGGCA GTGATGGTCA ACCAGGCCCT CCTGGTCCTC 2041 CTGGAACTGC CGGATTCCCT GGATCCCCTG GTGCTAAGGG TGAAGTTGGA CCTGCAGGGT 2101 CTCCTGGTTC AAATGGTGCC CCTGGACAAA GAGGAGAACC TGGACCTCAG GGACACGCTG 2161 GTGCTCAAGG TCCTCCTGGC CCTCCTGGGA TTAATGGTAG TCCTGGTGGT AAAGGCGAAA 2221 TGGGTCCCGC TGGCATTCCT GGAGCTCCTG GACTGATGGG AGCCCGGGGT CCTCCAGGAC 2281 CAGCCGGTGC TAATGGTGCT CCTGGACTGC GAGGTGGTGC AGGTGAGCCT GGTAAGAATG 2341 GTGCCAAAGG AGAGCCCGGA CCACGTGGTG AACGCGGTGA GGCTGGTATT CCAGGTGTTC 2401 CAGGAGCTAA AGGCGAAGAT GGCAAGGATG GATCACCTGG AGAACCTGGT GCAAATGGGC 2461 TTCCAGGAGC TGCAGGAGAA AGGGGTGCCC CTGGGTTCCG AGGACCTGCT GGACCAAATG 2521 GCATCCCAGG AGAAAAGGGT CCTGCTGGAG AGCGTGGTGC TCCAGGCCCT GCAGGGCCCA 2581 GAGGAGCTGC TGGAGAACCT GGCAGAGATG GCGTCCCTGG AGGTCCAGGA ATGAGGGGCA 2641 TGCCCGGAAG TCCAGGAGGA CCAGGAAGTG ATGGGAAACC AGGGCCTCCC GGAAGTCAAG 2701 GAGAAAGTGG TCGACCAGGT CCTCCTGGGC CATCTGGTCC CCGAGGTCAG CCTGGTGTCA 2761 TGGGCTTCCC CGGTCCTAAA GGAAATGATG GTGCTCCTGG TAAGAATGGA GAACGAGGTG 2821 GCCCTGGAGG ACCTGGCCCT CAGGGTCCTC CTGGAAAGAA TGGTGAAACT GGACCTCAGG 2881 GACCCCCAGG GCCTACTGGG CCTGGTGGTG ACAAAGGAGA CACAGGACCC CCTGGTCCAC 2941 AAGGATTACA AGGCTTGCCT GGTACAGGTG GTCCTCCAGG AGAAAATGGA AAACCTGGGG 3001 AACCAGGTCC AAAGGGTGAT GCCGGTGCAC CTGGAGCTCC AGGAGGCAAG GGTGATGCTG 3061 GTGCCCCTGG TGAACGTGGA CCTCCTGGAT TGGCAGGGGC CCCAGGACTT AGAGGTGGAG 3121 CTGGTCCCCC TGGTCCCGAA GGAGGAAAGG GTGCTGCTGG TCCTCCTGGG CCACCTGGTG 3181 CTGCTGGTAC TCCTGGTCTG CAAGGAATGC CTGGAGAAAG AGGAGGTCTT GGAAGTCCTG 3241 GTCCAAAGGG TGACAAGGGT GAACCAGGCG GTCCAGGTGC TGATGGTGTC CCAGGGAAAG 3301 ATGGCCCAAG GGGTCCTACT GGTCCTATTG GTCCTCCTGG CCCAGCTGGC CAGCCTGGAG 3361 ATAAGGGTGA AGGTGGTGCC CCCGGACTTC CAGGTATAGC TGGACCTCGT GGTAGCCCTG 3421 GTGAGAGAGG TGAAACTGGC CCTCCAGGAC CTGCTGGTTT CCCTGGTGCT CCTGGACAGA 3481 ATGGTGAACC TGGTGGTAAA GGAGAAAGAG GGGCTCCGGG TGAGAAAGGT GAAGGAGGCC 3541 CTCCTGGAGT TGCAGGACCC CCTGGAGGTT CTGGACCTGC TGGTCCTCCT GGTCCCCAAG 3601 GTGTCAAAGG TGAACGTGGC AGTCCTGGTG GACCTGGTGC TGCTGGCTTC CCTGGTGCTC 3661 GTGGTCTTCC TGGTCCTCCT GGTAGTAATG GTAACCCAGG ACCCCCAGGT CCCAGCGGTT 3721 CTCCAGGCAA GGATGGGCCC CCAGGTCCTG CGGGTAACAC TGGTGCTCCT GGCAGCCCTG 3781 GAGTGTCTGG ACCAAAAGGT GATGCTGGCC AACCAGGAGA GAAGGGATCG CCTGGTGCCC 3841 AGGGCCCACC AGGAGCTCCA GGCCCACTTG GGATTGCTGG GATCACTGGA GCACGGGGTC 3901 TTGCAGGACC ACCAGGCATG CCAGGTCCTA GGGGAAGCCC TGGCCCTCAG GGTGTCAAGG 3961 GTGAAAGTGG GAAACCAGGA GCTAACGGTC TCAGTGGAGA ACGTGGTCCC CCTGGACCCC 4021 AGGGTCTTCC TGGTCTGGCT GGTACAGCTG GTGAACCTGG AAGAGATGGA AACCCTGGAT 4081 CAGATGGTCT TCCAGGCCGA GATGGATCTC CTGGTGGCAA GGGTGATCGT GGTGAAAATG 4141 GCTCTCCTGG TGCCCCTGGT GCTCCTGGTC ATCCAGGCCC ACCTGGTCCT GTCGGTCCAG 4201 CTGGAAAGAG TGGTGACAGA GGAGAAAGTG GCCCTGCTGG CCCTGCTGGT GCTCCCGGTC 4261 CTGCTGGTTC CCGAGGTGCT CCTGGTCCTC AAGGCCCACG TGGTGACAAA GGTGAAACAG 4321 GTGAACGTGG AGCTGCTGGC ATCAAAGGAC ATCGAGGATT CCCTGGTAAT CCAGGTGCCC 4381 CAGGTTCTCC AGGCCCTGCT GGTCAGCAGG GTGCAATCGG CAGTCCAGGA CCTGCAGGCC 4441 CCAGAGGACC TGTTGGACCC AGTGGACCTC CTGGCAAAGA TGGAACCAGT GGACATCCAG 4501 GTCCCATTGG ACCACCAGGG CCTCGAGGTA ACAGAGGTGA AAGAGGATCT GAGGGCTCCC 4561 CAGGCCACCC AGGGCAACCA GGCCCTCCTG GACCTCCTGG TGCCCCTGGT CCTTGCTGTG 4621 GTGGTGTTGG AGCCGCTGCC ATTGCTGGGA TTGGAGGTGA AAAAGCTGGC GGTTTTGCCC 4681 CGTATTATGG AGATGAACCA ATGGATTTCA AAATCAACAC CGATGAGATT ATGACTTCAC 4741 TCAAGTCTGT TAATGGACAA ATAGAAAGCC TCATTAGTCC TGATGGTTCT CGTAAAAACC 4801 CCGCTAGAAA CTGCAGAGAC CTGAAATTCT GCCATCCTGA ACTCAAGAGT GGAGAATACT 4861 GGGTTGACCC TAACCAAGGA TGCAAATTGG ATGCTATCAA GGTATTCTGT AATATGGAAA 4921 CTGGGGAAAC ATGCATAAGT GCCAATCCTT TGAATGTTCC ACGGAAACAC TGGTGGACAG 4981 ATTCTAGTGC TGAGAAGAAA CACGTTTGGT TTGGAGAGTC CATGGATGGT GGTTTTCAGT 5041 TTAGCTACGG CAATCCTGAA CTTCCTGAAG ATGTCCTTGA TGTGCAGCTG GCATTCCTTC 5101 GACTTCTCTC CAGCCGAGCT TCCCAGAACA TCACATATCA CTGCAAAAAT AGCATTGCAT 5161 ACATGGATCA GGCCAGTGGA AATGTAAAGA AGGCCCTGAA GCTGATGGGG TCAAATGAAG 5221 GTGAATTCAA GGCTGAAGGA AATAGCAAAT TCACCTACAC AGTTCTGGAG GATGGTTGCA 5281 CGAAACACAC TGGGGAATGG AGCAAAACAG TCTTTGAATA TCGAACACGC AAGGCTGTGA 5341 GACTACCTAT TGTAGATATT GCACCCTATG ACATTGGTGG TCCTGATCAA GAATTTGGTG 5401 TGGACGTTGG CCCTGTTTGC TTTTTAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 5461 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 5521 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 5581 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 5641 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 5701 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 5761 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 5821 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 5881 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 5941 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 6001 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 6061 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 6121 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 6181 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 6241 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 6301 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 6361 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 6421 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 6481 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 6541 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 6601 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 6661 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 6721 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 6781 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 6841 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 6901 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 6961 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 7021 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 7081 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 7141 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 7201 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 7261 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 7321 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 7381 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 7441 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 7501 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 7561 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 7621 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 7681 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 7741 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 7801 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 7861 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 7921 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 7981 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 8041 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 8101 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 8161 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 8221 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 8281 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 8341 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 8401 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 8461 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 8521 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 8581 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 8641 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 8701 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 8761 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 8821 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 8881 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 8941 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 9001 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 9061 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 9121 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 9181 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 9241 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT //