LOCUS       RC211751                8817 bp    DNA     circular  SYN 
DEFINITION  RAD50 (Myc-DDK-tagged)-Human RAD50 homolog (S. cerevisiae) (RAD50)
ACCESSION   NM_005732
VERSION     NM_005732.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4974..5065 
                     /label="Myc-DDK"  
     misc_feature    5095..5680 
                     /label="PolyA signal"  
     misc_feature    5152..5171 
                     /label="Restriction Enzyme"  
     misc_feature    5956..6628 
                     /label="ColE1"  
     misc_feature    7058..7852 
                     /label="Kan/Neo"  
     misc_feature    7887..8189 
                     /label="SV40 ori"  
     misc_feature    8275..8752 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4956..4962 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4956 
                     /label="RAD50"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCGGATC GAAAAGATGA GCATTCTGGG CGTGCGGAGT TTTGGAATAG 
     1081 AGGACAAAGA TAAGCAAATT ATCACTTTCT TCAGCCCCCT TACAATTTTG GTTGGACCCA 
     1141 ATGGGGCGGG AAAGACGACC ATCATTGAAT GTCTAAAATA TATTTGTACT GGAGATTTCC 
     1201 CTCCTGGAAC CAAAGGAAAT ACATTTGTAC ACGATCCCAA GGTTGCTCAA GAAACAGATG 
     1261 TGAGAGCCCA GATTCGTCTG CAATTTCGTG ATGTCAATGG AGAACTTATA GCTGTGCAAA 
     1321 GATCTATGGT GTGTACTCAG AAAAGCAAAA AGACAGAATT TAAAACTCTG GAAGGAGTCA 
     1381 TTACTAGAAC AAAGCATGGT GAAAAGGTCA GTCTGAGCTC TAAGTGTGCA GAAATTGACC 
     1441 GAGAAATGAT CAGTTCTCTT GGGGTTTCCA AGGCTGTGCT AAATAATGTC ATTTTCTGTC 
     1501 ATCAAGAAGA TTCTAATTGG CCTTTAAGTG AAGGAAAGGC TTTGAAGCAA AAGTTTGATG 
     1561 AGATTTTTTC AGCAACAAGA TACATTAAAG CCTTAGAAAC ACTTCGGCAG GTACGTCAGA 
     1621 CACAAGGTCA GAAAGTAAAA GAATATCAAA TGGAACTAAA ATATCTGAAG CAATATAAGG 
     1681 AAAAAGCTTG TGAGATTCGT GATCAGATTA CAAGTAAGGA AGCCCAGTTA ACATCTTCAA 
     1741 AGGAAATTGT CAAATCCTAT GAGAATGAAC TTGATCCATT GAAGAATCGT CTAAAAGAAA 
     1801 TTGAACATAA TCTCTCTAAA ATAATGAAAC TTGACAATGA AATTAAAGCC TTGGATAGCC 
     1861 GAAAGAAGCA AATGGAGAAA GATAATAGTG AACTGGAAGA GAAAATGGAA AAGGTTTTTC 
     1921 AAGGGACTGA TGAGCAACTA AATGACTTAT ATCACAATCA CCAGAGAACA GTAAGGGAGA 
     1981 AAGAAAGGAA ATTGGTAGAC TGTCATCGTG AACTGGAAAA ACTAAATAAA GAATCTAGGC 
     2041 TTCTCAATCA GGAAAAATCA GAACTGCTTG TTGAACAGGG TCGTCTACAG CTGCAAGCAG 
     2101 ATCGCCATCA AGAACATATC CGAGCTAGAG ATTCATTAAT TCAGTCTTTG GCAACACAGC 
     2161 TAGAATTGGA TGGCTTTGAG CGTGGACCAT TCAGTGAAAG ACAGATTAAA AATTTTCACA 
     2221 AACTTGTGAG AGAGAGACAA GAAGGGGAAG CAAAAACTGC CAACCAACTG ATGAATGACT 
     2281 TTGCAGAAAA AGAGACTCTG AAACAAAAAC AGATAGATGA GATAAGAGAT AAGAAAACTG 
     2341 GACTGGGAAG AATAATTGAG TTAAAATCAG AAATCCTAAG TAAGAAGCAG AATGAGCTGA 
     2401 AAAATGTGAA GTATGAATTA CAGCAGTTGG AAGGATCTTC AGACAGGATT CTTGAACTGG 
     2461 ACCAGGAGCT CATAAAAGCT GAACGTGAGT TAAGCAAGGC TGAGAAAAAC AGCAATGTAG 
     2521 AAACCTTAAA AATGGAAGTA ATAAGTCTCC AAAATGAAAA AGCAGACTTA GACAGGACCC 
     2581 TGCGTAAACT TGACCAGGAG ATGGAGCAGT TAAACCATCA TACAACAACA CGTACCCAAA 
     2641 TGGAGATGCT GACCAAAGAC AAAGCTGACA AAGATGAACA AATCAGAAAA ATAAAATCTA 
     2701 GGCACAGTGA TGAATTAACC TCACTGTTGG GATATTTTCC CAACAAAAAA CAGCTTGAAG 
     2761 ACTGGCTACA TAGTAAATCA AAAGAAATTA ATCAGACCAG GGACAGACTT GCCAAATTGA 
     2821 ACAAGGAACT AGCTTCATCT GAGCAGAATA AAAATCATAT AAATAATGAA CTAAAAAGAA 
     2881 AGGAAGAGCA GTTGTCCAGT TACGAAGACA AGCTGTTTGA TGTTTGTGGT AGCCAGGATT 
     2941 TTGAAAGTGA TTTAGACAGG CTTAAAGAGG AAATTGAAAA ATCATCAAAA CAGCGAGCCA 
     3001 TGCTGGCTGG AGCCACAGCA GTTTACTCCC AGTTCATTAC TCAGCTAACA GACGAAAACC 
     3061 AGTCATGTTG CCCCGTTTGT CAGAGAGTTT TTCAGACAGA GGCTGAGTTA CAAGAAGTCA 
     3121 TCAGTGATTT GCAGTCTAAA CTGCGACTTG CTCCAGATAA ACTCAAGTCA ACAGAATCAG 
     3181 AGCTAAAAAA AAAGGAAAAG CGGCGTGATG AAATGCTGGG ACTTGTGCCC ATGAGGCAAA 
     3241 GCATAATTGA TTTGAAGGAG AAGGAAATAC CAGAATTAAG AAACAAACTG CAGAATGTCA 
     3301 ATAGAGACAT ACAGCGCCTA AAGAACGACA TAGAAGAACA AGAAACACTC TTGGGTACAA 
     3361 TAATGCCTGA AGAAGAAAGT GCCAAAGTAT GCCTGACAGA TGTTACAATT ATGGAGAGGT 
     3421 TCCAGATGGA ACTTAAAGAT GTTGAAAGAA AAATTGCACA ACAAGCAGCT AAGCTACAAG 
     3481 GAATAGACTT AGATCGAACT GTCCAACAAG TCAACCAGGA GAAACAAGAG AAACAGCACA 
     3541 AGTTAGACAC AGTTTCTAGT AAGATTGAAT TGAATCGTAA GCTTATACAG GACCAGCAGG 
     3601 AACAGATTCA ACATCTAAAA AGTACAACAA ATGAGCTAAA ATCTGAGAAA CTTCAGATAT 
     3661 CCACTAATTT GCAACGTCGT CAGCAACTGG AGGAGCAGAC TGTGGAATTA TCCACTGAAG 
     3721 TTCAGTCTTT GTACAGAGAG ATAAAGGATG CTAAAGAGCA GGTAAGCCCT TTGGAAACAA 
     3781 CATTGGAAAA GTTCCAGCAA GAAAAAGAAG AATTAATCAA CAAAAAAAAT ACAAGCAACA 
     3841 AAATAGCACA GGATAAACTG AATGATATTA AAGAGAAGGT TAAAAATATT CATGGCTATA 
     3901 TGAAAGACAT TGAGAATTAT ATTCAAGATG GGAAAGACGA CTATAAGAAG CAAAAAGAAA 
     3961 CTGAACTTAA TAAAGTAATA GCTCAACTAA GTGAATGCGA GAAACACAAA GAAAAGATAA 
     4021 ATGAAGATAT GAGACTCATG AGACAAGATA TTGATACACA GAAGATACAA GAAAGGTGGC 
     4081 TACAAGATAA CCTTACTTTA AGAAAAAGAA ATGAGGAACT AAAAGAAGTT GAAGAAGAAA 
     4141 GAAAACAACA TTTGAAGGAA ATGGGTCAAA TGCAGGTTTT GCAAATGAAA AGTGAACATC 
     4201 AGAAGTTGGA AGAGAACATA GACAATATAA AAAGAAATCA TAATTTGGCA TTAGGGCGAC 
     4261 AGAAAGGTTA TGAAGAAGAA ATTATTCATT TTAAGAAAGA ACTTCGAGAA CCACAATTTC 
     4321 GGGATGCTGA GGAAAAGTAT AGAGAAATGA TGATTGTTAT GAGGACAACA GAACTTGTGA 
     4381 ACAAGGATCT GGATATTTAT TATAAGACTC TTGACCAAGC AATAATGAAA TTTCACAGTA 
     4441 TGAAAATGGA AGAAATCAAT AAAATTATAC GTGACCTGTG GCGAAGTACC TATCGTGGAC 
     4501 AAGATATTGA ATACATAGAA ATACGGTCTG ATGCCGATGA AAATGTATCA GCTTCTGATA 
     4561 AAAGGCGGAA TTATAACTAC CGAGTGGTGA TGCTGAAGGG AGACACAGCC TTGGATATGC 
     4621 GAGGACGATG CAGTGCTGGA CAAAAGGTAT TAGCCTCACT CATCATTCGC CTGGCCCTGG 
     4681 CTGAAACGTT CTGCCTCAAC TGTGGCATCA TTGCCTTGGA TGAGCCAACA ACAAATCTTG 
     4741 ACCGAGAAAA CATTGAATCT CTTGCACATG CTCTGGTTGA GATAATAAAA AGTCGCTCAC 
     4801 AGCAGCGTAA CTTCCAGCTT CTGGTAATCA CTCATGATGA AGATTTTGTG GAGCTTTTAG 
     4861 GACGTTCTGA ATATGTGGAG AAATTCTACA GGATTAAAAA GAACATCGAT CAGTGCTCAG 
     4921 AGATTGTGAA ATGCAGTGTT AGCTCCCTGG GATTCAATGT TCATACGCGT ACGCGGCCGC 
     4981 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5041 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5101 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5161 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5221 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5281 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5341 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5401 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5461 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5521 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5581 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5641 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5701 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5761 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5821 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5881 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5941 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6001 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6061 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6121 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6181 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6241 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6301 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6361 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6421 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6481 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6541 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6601 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6661 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6721 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6781 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6841 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6901 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6961 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7021 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7081 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7141 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7201 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7261 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7321 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7381 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7441 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7501 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7561 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7621 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7681 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7741 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7801 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7861 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7921 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7981 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8041 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8221 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8281 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8341 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8401 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8461 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8521 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8581 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8641 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8701 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8761 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//