LOCUS       RC211700                8550 bp    DNA     circular  SYN 
DEFINITION  ATP8B2 (Myc-DDK-tagged)-Human ATPase, class I, type 8B, member 2 (ATP8B2), transcript variant 1
ACCESSION   NM_020452
VERSION     NM_020452.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4707..4798 
                     /label="Myc-DDK"  
     misc_feature    4828..5413 
                     /label="PolyA signal"  
     misc_feature    4885..4904 
                     /label="Restriction Enzyme"  
     misc_feature    5689..6361 
                     /label="ColE1"  
     misc_feature    6791..7585 
                     /label="Kan/Neo"  
     misc_feature    7620..7922 
                     /label="SV40 ori"  
     misc_feature    8008..8485 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4689..4695 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4689 
                     /label="ATP8B2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATACCTTG AGAGCTGTTC CCCTTTTTTC AATATCTGGC CTCTTCTCCT 
     1081 TTCCCTACAG GGTCTCCCAT GGGATTGCTG GGATCTTGCT GGGTGAGATG GCAGTGTGTG 
     1141 CAAAAAAGCG CCCCCCAGAA GAAGAAAGGA GGGCGCGGGC TAATGACCGA GAATACAATG 
     1201 AGAAATTCCA GTATGCGAGT AACTGCATCA AGACCTCCAA GTACAATATT CTCACCTTCC 
     1261 TGCCTGTCAA CCTCTTTGAG CAGTTCCAGG AAGTTGCCAA CACTTACTTC CTGTTCCTCC 
     1321 TCATTCTGCA GTTGATCCCC CAGATCTCTT CCCTGTCCTG GTTCACCACC ATTGTGCCTT 
     1381 TGGTTCTTGT CCTCACCATC ACAGCTGTTA AAGATGCCAC TGATGACTAT TTCCGCCACA 
     1441 AGAGCGATAA CCAGGTGAAT AACCGCCAGT CTCAGGTGCT GATCAATGGA ATCCTCCAGC 
     1501 AGGAGCAGTG GATGAATGTC TGTGTTGGTG ATATTATCAA GCTAGAAAAT AACCAGTTTG 
     1561 TGGCGGCGGA TCTCCTCCTC CTTTCCAGCA GTGAGCCCCA TGGGCTGTGT TACATAGAGA 
     1621 CAGCAGAACT TGATGGCGAG ACCAACATGA AAGTACGTCA GGCGATTCCA GTCACCTCAG 
     1681 AATTGGGAGA CATCAGTAAG CTTGCCAAGT TTGACGGTGA AGTGATCTGT GAACCTCCCA 
     1741 ACAACAAACT GGACAAATTC AGCGGAACCC TCTACTGGAA GGAAAATAAG TTCCCTCTGA 
     1801 GCAACCAGAA CATGCTGCTG CGGGGCTGTG TGCTGCGAAA CACCGAGTGG TGCTTCGGGC 
     1861 TGGTCATCTT TGCAGGTCCC GACACTAAGC TGATGCAAAA CAGCGGCAGA ACAAAGTTCA 
     1921 AAAGAACGAG TATCGATCGC CTAATGAATA CCCTGGTGCT CTGGATTTTT GGATTCCTGG 
     1981 TTTGCATGGG GGTGATCCTG GCCATTGGCA ATGCCATCTG GGAGCACGAG GTGGGGATGC 
     2041 GTTTCCAGGT CTACCTGCCG TGGGATGAGG CAGTGGACAG TGCCTTCTTC TCTGGCTTCC 
     2101 TCTCCTTCTG GTCCTACATC ATCATCCTCA ACACCGTTGT GCCCATTTCA CTCTATGTCA 
     2161 GTGTGGAGGT CATCCGTCTG GGCCACAGCT ACTTCATCAA CTGGGATAAG AAGATGTTCT 
     2221 GCATGAAGAA GCGGACGCCT GCAGAAGCCC GCACCACCAC CCTAAACGAG GAGCTGGGCC 
     2281 AGGTGGAGTA CATCTTCTCC GACAAGACGG GCACCCTCAC CCAGAACATC ATGGTTTTCA 
     2341 ACAAGTGCTC CATCAATGGC CACAGCTATG GTGATGTGTT TGACGTCCTG GGACACAAAG 
     2401 CTGAATTGGG AGAGAGGCCT GAACCTGTTG ACTTCTCCTT CAATCCTCTG GCTGACAAGA 
     2461 AGTTCTTATT TTGGGACCCC AGCCTGCTGG AGGCTGTCAA GATCGGGGAC CCCCACACGC 
     2521 ATGAGTTCTT CCGCCTCCTT TCCCTGTGTC ATACTGTCAT GTCAGAAGAA AAGAACGAAG 
     2581 GAGAGCTGTA CTACAAAGCT CAGTCCCCAG ATGAGGGGGC CCTGGTCACC GCAGCCAGGA 
     2641 ACTTTGGTTT TGTTTTCCGC TCTCGCACCC CCAAAACAAT CACCGTCCAT GAGATGGGCA 
     2701 CAGCCATCAC CTACCAGCTG CTGGCCATCC TGGACTTCAA CAACATCCGC AAGCGGATGT 
     2761 CGGTCATAGT GCGGAATCCA GAGGGGAAGA TCCGACTCTA CTGCAAAGGG GCTGACACTA 
     2821 TCCTACTGGA CAGACTGCAC CACTCCACTC AAGAGCTGCT CAACACCACC ATGGACCACC 
     2881 TTAATGAGTA CGCAGGGGAA GGGCTGAGGA CCCTGGTGCT GGCCTACAAG GATCTGGATG 
     2941 AAGAGTACTA CGAGGAGTGG GCTGAGCGAC GCCTCCAGGC CAGCCTGGCC CAGGACAGCC 
     3001 GGGAGGACAG GCTGGCTAGC ATCTATGAGG AGGTTGAGAA CAACATGATG CTGCTGGGTG 
     3061 CAACGGCCAT TGAGGACAAA CTTCAGCAAG GGGTTCCAGA GACCATTGCC CTCCTGACAC 
     3121 TGGCCAACAT CAAGATTTGG GTGCTAACCG GAGACAAGCA AGAGACGGCT GTGAACATCG 
     3181 GCTATTCCTG CAAGATGCTG ACGGATGACA TGACTGAGGT TTTCATAGTC ACTGGCCATA 
     3241 CTGTCCTGGA GGTGCGGGAG GAGCTCAGGA AAGCCCGGGA GAAGATGATG GACTCATCCC 
     3301 GCTCCGTAGG CAACGGCTTC ACCTATCAGG ACAAGCTTTC TTCTTCCAAG CTAACTTCTG 
     3361 TCCTGGAGGC CGTTGCTGGG GAGTACGCCC TGGTCATAAA TGGTCACAGC CTGGCCCACG 
     3421 CACTGGAGGC AGACATGGAG CTGGAGTTTC TGGAGACAGC GTGTGCCTGC AAAGCTGTCA 
     3481 TCTGCTGCCG GGTGACCCCC TTGCAGAAGG CACAGGTGGT AGAACTGGTC AAGAAGTACA 
     3541 AGAAGGCTGT GACGCTTGCC ATTGGAGACG GAGCCAATGA TGTCAGCATG ATCAAAACGG 
     3601 CTCACATTGG TGTGGGGATC AGTGGGCAGG AAGGGATCCA GGCTGTCTTG GCCTCCGATT 
     3661 ACTCCTTCTC CCAGTTCAAG TTCCTGCAGC GCCTCCTGCT GGTGCATGGG CGCTGGTCCT 
     3721 ACCTGCGAAT GTGCAAGTTT CTTTGCTATT TCTTCTACAA AAACTTTGCT TTCACCATGG 
     3781 TCCACTTCTG GTTTGGCTTC TTCTGTGGCT TCTCAGCCCA GACCGTCTAT GACCAGTATT 
     3841 TCATCACCCT GTATAACATC GTGTACACCT CCCTGCCAGT CCTGGCTATG GGGGTCTTTG 
     3901 ATCAGGATGT CCCCGAGCAG CGGAGCATGG AGTACCCTAA GCTGTATGAG CCGGGCCAGC 
     3961 TGAACCTTCT CTTCAACAAG CGGGAGTTCT TCATCTGCAT CGCCCAGGGC ATCTACACCT 
     4021 CCGTGCTCAT GTTCTTCATT CCCTATGGGG TGTTTGCTGA TGCCACCCGG GATGATGGCA 
     4081 CTCAGCTGGC TGACTACCAG TCCTTTGCAG TCACTGTGGC CACATCCTTG GTCATTGTGG 
     4141 TTAGCGTGCA GATTGGGCTC GACACAGGCT ACTGGACGGC CATCAACCAC TTCTTCATCT 
     4201 GGGGAAGCCT TGCTGTTTAC TTTGCCATCC TCTTTGCCAT GCACAGCAAT GGGCTCTTCG 
     4261 ACATGTTTCC CAACCAGTTC CGGTTTGTGG GGAATGCCCA GAACACCTTG GCCCAGCCCA 
     4321 CGGTGTGGCT GACCATTGTG CTCACCACAG TCGTCTGCAT CATGCCCGTG GTTGCCTTCC 
     4381 GATTCCTCAG GCTCAACCTG AAGCCGGATC TCTCCGACAC GGTCCGCTAC ACACAGCTCG 
     4441 TGAGGAAGAA GCAGAAGGCC CAGCACCGCT GCATGCGGCG GGTTGGCCGC ACTGGCTCCC 
     4501 GGCGCTCCGG CTATGCCTTC TCCCATCAGG AGGGCTTCGG GGAGCTCATC ATGTCTGGCA 
     4561 AGAACATGCG GCTGAGCTCT CTCGCGCTCT CCAGCTTCAC CACCCGCTCC AGCTCCAGCT 
     4621 GGATTGAGAG CCTGCGCAGG AAGAAGAGTG ACAGTGCCAG TAGCCCCAGT GGCGGTGCCG 
     4681 ACAAGCCCCT CAAGGGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4741 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4801 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4861 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4921 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4981 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5041 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5101 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5161 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5221 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5281 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5341 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5401 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5461 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5521 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5581 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5641 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5701 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5761 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5821 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5881 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5941 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6001 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6061 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6121 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6181 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6241 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6301 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6361 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6421 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6481 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6541 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6601 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6661 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6721 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6781 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6841 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6901 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6961 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7021 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7081 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7141 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7201 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7261 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7321 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7381 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7441 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7501 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7561 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7621 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7681 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7741 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7801 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7861 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7981 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8041 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8101 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8161 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8221 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8281 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8341 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8401 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8461 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8521 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//