LOCUS       RC211685                8649 bp    DNA     circular  SYN 
DEFINITION  LTBP3 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 3 (LTBP3), transcript variant 2
ACCESSION   NM_021070
VERSION     NM_021070.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="LTBP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGGCCC CGAGGGGCTG CTGGCGGCCT GGCCCCTGAG ATGCGCGGGG 
     1081 CGGGGGCGGC GGGGCTGCTG GCGCTGCTGC TGCTGCTGCT GCTGCTGCTG CTGGGCCTGG 
     1141 GCGGCAGGGT CGAGGGGGGG CCGGCCGGCG AGCGGGGCGC AGGCGGGGGC GGGGCGCTGG 
     1201 CCCGCGAGCG CTTCAAGGTG GTCTTTGCGC CGGTGATCTG CAAGCGGACC TGTCTCAAGG 
     1261 GCCAGTGTCG GGACAGTTGT CAGCAGGGCT CCAACATGAC GCTCATCGGA GAGAACGGCC 
     1321 ACAGCACAGA CACGCTCACG GGCTCCGGCT TCCGCGTGGT GGTGTGCCCT CTCCCCTGCA 
     1381 TGAATGGCGG CCAGTGCTCC TCGCGAAACC AGTGCCTGTG TCCCCCGGAC TTCACTGGGC 
     1441 GCTTCTGCCA GGTGCCCGCA GGAGGAGCCG GTGGGGGTAC CGGCGGCTCA GGCCCCGGCC 
     1501 TGAGCAGGAC AGGGGCCCTG TCCACAGGGG CGCTGCCGCC CCTGGCTCCG GAGGGCGACT 
     1561 CTGTGGCCAG CAAGCACGCC ATCTACGCCG TCCAGGTGAT CGCTGACCCT CCTGGGCCCG 
     1621 GGGAGGGGCC TCCTGCCCAG CACGCAGCCT TCCTGGTGCC CCTAGGCCCG GGACAGATCT 
     1681 CAGCAGAAGT GCAGGCCCCG CCCCCCGTGG TGAATGTGCG CGTCCATCAC CCGCCCGAGG 
     1741 CCTCAGTCCA GGTGCACCGC ATTGAGAGCT CGAACGCCGA GAGCGCAGCC CCCTCCCAGC 
     1801 ACCTGCTGCC GCACCCCAAG CCCTCGCACC CCCGGCCGCC CACCCAGAAG CCCCTGGGCC 
     1861 GCTGCTTTCA GGACACTCTG CCCAAGCAGC CGTGTGGCAG CAACCCCCTC CCCGGCCTCA 
     1921 CCAAGCAGGA AGACTGCTGC GGTAGCATCG GCACTGCCTG GGGCCAGAGC AAGTGCCACA 
     1981 AGTGTCCCCA GCTGCAGTAC ACAGGAGTGC AGAAGCCAGG GCCTGTACGT GGGGAAGTGG 
     2041 GCGCTGACTG TCCCCAGGGC TACAAGAGGC TTAACAGCAC CCACTGCCAG GACATCAACG 
     2101 AGTGCGCAAT GCCGGGCGTG TGTCGCCATG GTGACTGCCT CAACAACCCT GGCTCCTATC 
     2161 GCTGTGTCTG CCCACCTGGC CATAGTTTAG GCCCCTCCCG TACACAGTGC ATTGCAGACA 
     2221 AACCGGAGGA GAAGAGCCTG TGTTTCCGCC TGGTGAGCCC TGAGCACCAG TGCCAGCACC 
     2281 CACTGACCAC CCGCCTGACC CGCCAGCTCT GCTGCTGCAG TGTCGGCAAG GCCTGGGGCG 
     2341 CGCGGTGTCA GCGCTGCCCA ACAGATGGCA CCGCTGCGTT CAAGGAGATC TGCCCAGCTG 
     2401 GGAAGGGATA CCACATTCTC ACCTCCCACC AGACGCTCAC CATTCAGGGC GAGAGTGACT 
     2461 TTTCCCTTTT CCTGCACCCT GACGGGCCAC CCAAGCCCCA GCAGCTTCCG GAGAGCCCTA 
     2521 GCCAGGCTCC ACCACCTGAG GACACAGAGG AAGAGAGAGG GGTGACCACG GACTCACCGG 
     2581 TGAGTGAGGA GAGGTCAGTG CAGCAGAGCC ACCCAACTGC CACCACGACT CCTGCCCGGC 
     2641 CCTACCCCGA GCTGATCTCC CGTCCCTCGC CCCCGACCAT GCGCTGGTTC CTGCCGGACT 
     2701 TGCCTCCTTC CCGCAGCGCC GTAGAGATCG CTCCCACTCA GGTCACAGAG ACTGATGAGT 
     2761 GCCGACTGAA CCAGAACATC TGTGGCCACG GAGAGTGCGT GCCGGGCCCC CCTGACTACT 
     2821 CCTGCCACTG CAACCCCGGC TACCGGTCAC ATCCCCAGCA CCGCTACTGC GTGGATGTGA 
     2881 ACGAGTGCGA GGCAGAGCCC TGTGGCCCGG GGAGGGGCAT CTGCATGAAC ACCGGCGGCT 
     2941 CCTACAATTG CCACTGCAAC CGCGGCTACC GCCTGCACGT GGGCGCCGGG GGGCGCTCGT 
     3001 GCGTGGACCT GAACGAATGC GCCAAGCCCC ACCTGTGCGG CGACGGCGGC TTCTGCATCA 
     3061 ACTTTCCCGG TCACTACAAG TGCAACTGCT ACCCCGGCTA CCGGCTCAAA GCCTCCCGGC 
     3121 CTCCTGTGTG CGAAGACATC GACGAGTGCC GGGACCCAAG CTCTTGCCCG GATGGCAAAT 
     3181 GCGAGAACAA GCCCGGGAGC TTCAAGTGCA TCGCCTGTCA GCCTGGCTAC CGCAGCCAGG 
     3241 GGGGCGGGGC CTGTCGCGAC GTGAACGAGT GCGCCGAGGG CAGCCCCTGC TCGCCTGGCT 
     3301 GGTGCGAGAA CCTCCCGGGC TCCTTCCGCT GCACCTGTGC CCAGGGCTAC GCGCCCGCGC 
     3361 CCGACGGCCG CAGTTGCTTG GATGTGGACG AGTGTGAGGC TGGGGACGTG TGTGACAATG 
     3421 GCATCTGCAG CAACACGCCA GGATCTTTCC AGTGTCAGTG CCTCTCTGGC TACCATCTGT 
     3481 CCAGGGACCG GAGCCACTGC GAGGACATTG ATGAGTGTGA CTTCCCTGCA GCCTGCATTG 
     3541 GGGGTGACTG CATCAATACC AATGGCTCCT ACAGATGTCT TTGCCCCCAG GGGCATCGGC 
     3601 TGGTGGGTGG CAGGAAATGC CAAGACATAG ATGAGTGCAG CCAGGACCCG AGCCTGTGCC 
     3661 TTCCCCATGG GGCCTGCAAG AACCTTCAGG GCTCCTATGT GTGTGTCTGC GATGAGGGCT 
     3721 TCACTCCCAC CCAGGACCAG CACGGTTGTG AGGAGGTGGA GCAGCCCCAC CACAAGAAGG 
     3781 AGTGCTACCT GAACTTCGAT GACACAGTGT TCTGCGACAG CGTATTGGCC ACCAACGTGA 
     3841 CCCAGCAGGA GTGCTGCTGC TCTCTGGGGG CCGGCTGGGG CGACCACTGC GAAATCTACC 
     3901 CCTGCCCAGT CTACAGCTCA GCCGAGTTCC ACAGCCTCTG CCCAGACGGA AAGGGCTACA 
     3961 CCCAGGACAA CAACATCGTC AACTACGGCA TCCCAGCCCA CCGTGACATC GACGAGTGCA 
     4021 TGTTGTTCGG GTCGGAGATT TGCAAGGAGG GCAAGTGCGT GAACACGCAG CCTGGCTACG 
     4081 AGTGCTACTG CAAGCAGGGC TTCTACTACG ACGGGAACCT GCTGGAATGC GTGGACGTGG 
     4141 ACGAGTGCCT GGACGAGTCC AACTGCCGGA ACGGAGTGTG TGAGAACACG CGCGGCGGCT 
     4201 ACCGCTGTGC CTGCACGCCC CCTGCCGAGT ACAGTCCCGC GCAGCGCCAG TGCCTGAGCC 
     4261 CGGAAGAGAT GGAGCGTGCC CCGGAGCGGC GCGACGTGTG CTGGAGCCAG CGCGGAGAGG 
     4321 ACGGCATGTG CGCTGGCCCC CTGGCCGGGC CTGCCCTCAC CTTCGACGAC TGCTGCTGCC 
     4381 GCCAGGGCCG CGGCTGGGGC GCCCAATGCC GACCGTGCCC GCCGCGCGGC GCGGGGTCCC 
     4441 ATTGCCCGAC ATCGCAGAGC GAGAGCAATT CCTTCTGGGA CACAAGCCCC CTGCTGTTGG 
     4501 GGAAGCCCCC AAGAGATGAG GACAGTTCAG AGGAGGATTC AGACGAGTGT CGCTGCGTGA 
     4561 GTGGCCGCTG CGTGCCGCGG CCGGGCGGCG CCGTGTGCGA GTGTCCCGGC GGCTTCCAGC 
     4621 TCGACGCCTC CCGCGCCCGC TGCGTGGATA TCGACGAGTG CCGAGAGCTG AACCAGCGCG 
     4681 GGCTGCTGTG CAAGAGCGAG CGCTGCGTGA ACACCAGCGG CTCCTTCCGC TGCGTCTGCA 
     4741 AAGCCGGCTT CGCGCGCAGC CGCCCGCACG GGGCCTGCGT TCCCCAGCGC CGCCGCACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//