LOCUS       RC211665                7644 bp    DNA     circular  SYN 
DEFINITION  KSR2 (Myc-DDK-tagged)-Human kinase suppressor of ras 2 (KSR2)
ACCESSION   NM_173598
VERSION     NM_173598.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3801..3892 
                     /label="Myc-DDK"  
     misc_feature    3922..4507 
                     /label="PolyA signal"  
     misc_feature    3979..3998 
                     /label="Restriction Enzyme"  
     misc_feature    4783..5455 
                     /label="ColE1"  
     misc_feature    5885..6679 
                     /label="Kan/Neo"  
     misc_feature    6714..7016 
                     /label="SV40 ori"  
     misc_feature    7102..7579 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3783..3789 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3783 
                     /label="KSR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGACTTG AGCATCTCCA ACCTGGAAGG GCTTAGGACC AAATGTGCTA 
     1081 CCTCCAACGA CCTCACACAA AAAGAAATCC GGACCCTGGA GAGCAAGCTG GTGAAGTACT 
     1141 TCAGCCGGCA GCTGTCCTGC AAAAAGAAGG TAGCCTTGCA GGAGCGCAAC GCGGAGCTGG 
     1201 ACGGCTTCCC CCAGCTACGG CACTGGTTCC GAATCGTCGA TGTGCGCAAG GAGGTCCTGG 
     1261 AGGAAATCTC CCCCGGCCAG CTGAGCCTGG AGGACCTCTT GGAGATGACG GATGAACAGG 
     1321 TGTGCGAGAC TGTGGAGAAA TACGGAGCCA ACCGGGAGGA GTGTGCCCGC CTCAACGCCT 
     1381 CCCTCTCCTG CCTCAGGAAT GTCCACATGT CAGGAGGCAA CCTTTCCAAA CAAGACTGGA 
     1441 CCATCCAGTG GCCCACGACA GAGACGGGGA AGGAGAACAA TCCCGTGTGC CCCCCGGAGC 
     1501 CCACCCCGTG GATCCGCACC CATCTCTCCC AGAGCCCCAG GGTCCCGTCC AAGTGCGTCC 
     1561 AGCACTATTG TCACACCAGC CCCACTCCCG GGGCCCCTGT GTACACCCAC GTGGACAGGC 
     1621 TTACCGTGGA CGCCTACCCG GGCTTGTGCC CGCCCCCGCC ACTGGAGTCG GGCCACCGTT 
     1681 CCCTGCCCCC ATCGCCCCGG CAGCGGCACG CGGTCCGCAC CCCGCCGCGC ACCCCCAACA 
     1741 TCGTCACCAC CGTGACCCCG CCGGGCACGC CGCCCATGAG GAAGAAGAAC AAGCTGAAGC 
     1801 CCCCGGGGAC CCCACCGCCC TCCTCCCGAA AACTGATACA CTTGATCCCG GGATTCACCG 
     1861 CGCTGCATCG GAGCAAATCC CACGAGTTCC AGCTGGGGCA CCGCGTGGAC GAGGCCCACA 
     1921 CGCCCAAAGC CAAGAAGAAG AGCAAACCCT TGAACCTCAA GATCCACAGC AGCGTAGGCA 
     1981 GCTGCGAGAA CATCCCCTCT CAGCAGCGCT CCCCGCTGCT GTCCGAGCGC TCCCTCCGCT 
     2041 CCTTCTTTGT GGGACACGCA CCTTTCCTGC CTTCCACCCC TCCTGTTCAC ACTGAGGCCA 
     2101 ACTTCTCTGC AAACACACTG TCAGTGCCAC GCTGGTCCCC GCAGATCCCT CGCAGAGATC 
     2161 TCGGCAACTC CATCAAGCAC AGGTTTTCCA CCAAGTACTG GATGTCTCAG ACGTGCACAG 
     2221 TCTGTGGGAA AGGGATGCTT TTTGGCCTCA AGTGTAAAAA CTGCAAGTTA AAGTGCCACA 
     2281 ACAAATGCAC CAAAGAAGCC CCACCCTGTC ATCTTCTGAT CATCCACCGA GGAGATCCAG 
     2341 CAAGGTTAGT CCGGACAGAG TCCGTTCCGT GTGACATCAA CAACCCTCTA CGGAAGCCAC 
     2401 CTCGCTATTC AGACCTGCAC ATCAGTCAGA CGCTCCCCAA AACCAACAAA ATCAACAAGG 
     2461 ACCACATCCC TGTCCCTTAC CAGCCAGACT CCAGCAGCAA CCCCTCCTCC ACGACGTCCT 
     2521 CCACGCCCTC CTCGCCAGCA CCCCCCCTCC CTCCTAGTGC CACGCCGCCT TCTCCCCTAC 
     2581 ACCCTTCCCC ACAGTGCACA CGGCAGCAGA AGAACTTCAA CCTGCCAGCA TCCCACTACT 
     2641 ACAAATACAA GCAGCAGTTC ATCTTCCCAG ATGTGGTGCC GGTGCCGGAG ACGCCGACCC 
     2701 GGGCGCCCCA GGTCATCCTG CATCCGGTGA CCTCGAATCC AATCTTGGAA GGAAATCCAT 
     2761 TACTTCAAAT TGAAGTGGAG CCAACGTCGG AGAATGAAGA GGTCCATGAT GAGGCCGAAG 
     2821 AGTCAGAGGA TGACTTCGAG GAGATGAACC TGTCCCTCCT CTCGGCCCGG AGCTTCCCAC 
     2881 GCAAGGCCAG CCAGACCAGC ATCTTCCTTC AGGAGTGGGA CATCCCCTTT GAGCAGCTGG 
     2941 AGATCGGCGA GCTCATTGGA AAGGGCCGCT TTGGGCAAGT GTACCACGGC CGCTGGCATG 
     3001 GCGAGGTGGC CATCCGGCTG ATTGACATTG AGAGGGACAA CGAGGACCAG CTCAAGGCCT 
     3061 TCAAGCGGGA GGTGATGGCC TACAGGCAGA CACGGCATGA GAACGTGGTG CTTTTCATGG 
     3121 GTGCCTGCAT GAGCCCGCCT CACCTGGCCA TCATCACCAG CCTCTGTAAG GGACGGACGC 
     3181 TCTATTCCGT TGTGAGGGAT GCCAAAATCG TTTTGGATGT CAACAAAACC AGGCAGATTG 
     3241 CTCAAGAAAT TGTGAAGGGC ATGGGCTACC TCCACGCCAA GGGAATCCTA CACAAGGACC 
     3301 TCAAGTCAAA GAACGTCTTC TATGACAACG GCAAAGTGGT CATCACGGAC TTTGGACTCT 
     3361 TCAGCATTTC TGGGGTGCTG CAGGCTGGCA GGCGGGAGGA CAAACTGCGC ATCCAGAATG 
     3421 GCTGGCTATG CCACCTGGCA CCAGAGATCA TCCGCCAGCT GTCCCCCGAC ACAGAGGAGG 
     3481 ATAAGCTCCC CTTCTCCAAG CACTCTGACG TCTTTGCCCT TGGCACAATC TGGTATGAAC 
     3541 TCCACGCCAG GGAATGGCCT TTCAAGACCC AACCAGCAGA GGCAATAATC TGGCAAATGG 
     3601 GCACAGGCAT GAAACCCAAC CTCAGCCAGA TTGGCATGGG AAAAGAAATC TCGGACATTC 
     3661 TTCTCTTCTG CTGGGCCTTT GAACAAGAAG AGAGACCTAC CTTCACCAAG CTCATGGACA 
     3721 TGCTGGAGAA ACTGCCAAAG CGAAACCGTC GCCTGTCTCA CCCTGGACAT TTCTGGAAGT 
     3781 CTGCAGAGCT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3841 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4621 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4681 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4741 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4861 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4921 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4981 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5041 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5161 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5221 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5281 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5341 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5401 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5461 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5521 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5581 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5641 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5701 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5761 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5821 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5881 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5941 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6001 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6061 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6121 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6181 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6241 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6301 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6361 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6421 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6481 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6541 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6601 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6661 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6721 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6781 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6841 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7081 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7201 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7621 TAACAAAAAT TTAACGCGAA TTTT
//