LOCUS       RC211635                8694 bp    DNA     circular  SYN 
DEFINITION  BCR (Myc-DDK-tagged)-Human breakpoint cluster region (BCR), transcript variant 1
ACCESSION   NM_004327
VERSION     NM_004327.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4851..4942 
                     /label="Myc-DDK"  
     misc_feature    4972..5557 
                     /label="PolyA signal"  
     misc_feature    5029..5048 
                     /label="Restriction Enzyme"  
     misc_feature    5833..6505 
                     /label="ColE1"  
     misc_feature    6935..7729 
                     /label="Kan/Neo"  
     misc_feature    7764..8066 
                     /label="SV40 ori"  
     misc_feature    8152..8629 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4833..4839 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4833 
                     /label="BCR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGACCCG GTGGGCTTCG CGGAGGCGTG GAAGGCGCAG TTCCCGGACT 
     1081 CAGAGCCCCC GCGCATGGAG CTGCGCTCAG TGGGCGACAT CGAGCAGGAG CTGGAGCGCT 
     1141 GCAAGGCCTC CATTCGGCGC CTGGAGCAGG AGGTGAACCA GGAGCGCTTC CGCATGATCT 
     1201 ACCTGCAGAC GTTGCTGGCC AAGGAAAAGA AGAGCTATGA CCGGCAGCGA TGGGGCTTCC 
     1261 GGCGCGCGGC GCAGGCCCCC GACGGCGCCT CCGAGCCCCG AGCGTCCGCG TCGCGCCCGC 
     1321 AGCCAGCGCC CGCCGACGGA GCCGACCCGC CGCCCGCCGA GGAGCCCGAG GCCCGGCCCG 
     1381 ACGGCGAGGG TTCTCCGGGT AAGGCCAGGC CCGGGACCGC CCGCAGGCCC GGGGCAGCCG 
     1441 CGTCGGGGGA ACGGGACGAC CGGGGACCCC CCGCCAGCGT GGCGGCGCTC AGGTCCAACT 
     1501 TCGAGCGGAT CCGCAAGGGC CATGGCCAGC CCGGGGCGGA CGCCGAGAAG CCCTTCTACG 
     1561 TGAACGTCGA GTTTCACCAC GAGCGCGGCC TGGTGAAGGT CAACGACAAA GAGGTGTCGG 
     1621 ACCGCATCAG CTCCCTGGGC AGCCAGGCCA TGCAGATGGA GCGCAAAAAG TCCCAGCACG 
     1681 GCGCGGGCTC GAGCGTGGGG GATGCATCCA GGCCCCCTTA CCGGGGACGC TCCTCGGAGA 
     1741 GCAGCTGCGG CGTCGACGGC GACTACGAGG ACGCCGAGTT GAACCCCCGC TTCCTGAAGG 
     1801 ACAACCTGAT CGACGCCAAT GGCGGTAGCA GGCCCCCTTG GCCGCCCCTG GAGTACCAGC 
     1861 CCTACCAGAG CATCTACGTC GGGGGCATGA TGGAAGGGGA GGGCAAGGGC CCGCTCCTGC 
     1921 GCAGCCAGAG CACCTCTGAG CAGGAGAAGC GCCTTACCTG GCCCCGCAGG TCCTACTCCC 
     1981 CCCGGAGTTT TGAGGATTGC GGAGGCGGCT ATACCCCGGA CTGCAGCTCC AATGAGAACC 
     2041 TCACCTCCAG CGAGGAGGAC TTCTCCTCTG GCCAGTCCAG CCGCGTGTCC CCAAGCCCCA 
     2101 CCACCTACCG CATGTTCCGG GACAAAAGCC GCTCTCCCTC GCAGAACTCG CAACAGTCCT 
     2161 TCGACAGCAG CAGTCCCCCC ACGCCGCAGT GCCATAAGCG GCACCGGCAC TGCCCGGTTG 
     2221 TCGTGTCCGA GGCCACCATC GTGGGCGTCC GCAAGACCGG GCAGATCTGG CCCAACGATG 
     2281 GCGAGGGCGC CTTCCATGGA GACGCAGATG GCTCGTTCGG AACACCACCT GGATACGGCT 
     2341 GCGCTGCAGA CCGGGCAGAG GAGCAGCGCC GGCACCAAGA TGGGCTGCCC TACATTGATG 
     2401 ACTCGCCCTC CTCATCGCCC CACCTCAGCA GCAAGGGCAG GGGCAGCCGG GATGCGCTGG 
     2461 TCTCGGGAGC CCTGGAGTCC ACTAAAGCGA GTGAGCTGGA CTTGGAAAAG GGCTTGGAGA 
     2521 TGAGAAAATG GGTCCTGTCG GGAATCCTGG CTAGCGAGGA GACTTACCTG AGCCACCTGG 
     2581 AGGCACTGCT GCTGCCCATG AAGCCTTTGA AAGCCGCTGC CACCACCTCT CAGCCGGTGC 
     2641 TGACGAGTCA GCAGATCGAG ACCATCTTCT TCAAAGTGCC TGAGCTCTAC GAGATCCACA 
     2701 AGGAGTTCTA TGATGGGCTC TTCCCCCGCG TGCAGCAGTG GAGCCACCAG CAGCGGGTGG 
     2761 GCGACCTCTT CCAGAAGCTG GCCAGCCAGC TGGGTGTGTA CCGGGCCTTC GTGGACAACT 
     2821 ACGGAGTTGC CATGGAAATG GCTGAGAAGT GCTGTCAGGC CAATGCTCAG TTTGCAGAAA 
     2881 TCTCCGAGAA CCTGAGAGCC AGAAGCAACA AAGATGCCAA GGATCCAACG ACCAAGAACT 
     2941 CTCTGGAAAC TCTGCTCTAC AAGCCTGTGG ACCGTGTGAC GAGGAGCACG CTGGTCCTCC 
     3001 ATGACTTGCT GAAGCACACT CCTGCCAGCC ACCCTGACCA CCCCTTGCTG CAGGACGCCC 
     3061 TCCGCATCTC ACAGAACTTC CTGTCCAGCA TCAATGAGGA GATCACACCC CGACGGCAGT 
     3121 CCATGACGGT GAAGAAGGGA GAGCACCGGC AGCTGCTGAA GGACAGCTTC ATGGTGGAGC 
     3181 TGGTGGAGGG GGCCCGCAAG CTGCGCCACG TCTTCCTGTT CACCGACCTG CTTCTCTGCA 
     3241 CCAAGCTCAA GAAGCAGAGC GGAGGCAAAA CGCAGCAGTA TGACTGCAAA TGGTACATTC 
     3301 CGCTCACGGA TCTCAGCTTC CAGATGGTGG ATGAACTGGA GGCAGTGCCC AACATCCCCC 
     3361 TGGTGCCCGA TGAGGAGCTG GACGCTTTGA AGATCAAGAT CTCCCAGATC AAGAATGACA 
     3421 TCCAGAGAGA GAAGAGGGCG AACAAGGGCA GCAAGGCTAC GGAGAGGCTG AAGAAGAAGC 
     3481 TGTCGGAGCA GGAGTCACTG CTGCTGCTTA TGTCTCCCAG CATGGCCTTC AGGGTGCACA 
     3541 GCCGCAACGG CAAGAGTTAC ACGTTCCTGA TCTCCTCTGA CTATGAGCGT GCAGAGTGGA 
     3601 GGGAGAACAT CCGGGAGCAG CAGAAGAAGT GTTTCAGAAG CTTCTCCCTG ACATCCGTGG 
     3661 AGCTGCAGAT GCTGACCAAC TCGTGTGTGA AACTCCAGAC TGTCCACAGC ATTCCGCTGA 
     3721 CCATCAATAA GGAAGATGAT GAGTCTCCGG GGCTCTATGG GTTTCTGAAT GTCATCGTCC 
     3781 ACTCAGCCAC TGGATTTAAG CAGAGTTCAA ATCTGTACTG CACCCTGGAG GTGGATTCCT 
     3841 TTGGGTATTT TGTGAATAAA GCAAAGACGC GCGTCTACAG GGACACAGCT GAGCCAAACT 
     3901 GGAACGAGGA ATTTGAGATA GAGCTGGAGG GCTCCCAGAC CCTGAGGATA CTGTGCTATG 
     3961 AAAAGTGTTA CAACAAGACG AAGATCCCCA AGGAGGACGG CGAGAGCACG GACAGACTCA 
     4021 TGGGGAAGGG CCAGGTCCAG CTGGACCCGC AGGCCCTGCA GGACAGAGAC TGGCAGCGCA 
     4081 CCGTCATCGC CATGAATGGG ATCGAAGTAA AGCTCTCGGT CAAGTTCAAC AGCAGGGAGT 
     4141 TCAGCTTGAA GAGGATGCCG TCCCGAAAAC AGACAGGGGT CTTCGGAGTC AAGATTGCTG 
     4201 TGGTCACCAA GAGAGAGAGG TCCAAGGTGC CCTACATCGT GCGCCAGTGC GTGGAGGAGA 
     4261 TCGAGCGCCG AGGCATGGAG GAGGTGGGCA TCTACCGCGT GTCCGGTGTG GCCACGGACA 
     4321 TCCAGGCACT GAAGGCAGCC TTCGACGTCA ATAACAAGGA CGTGTCGGTG ATGATGAGCG 
     4381 AGATGGACGT GAACGCCATC GCAGGCACGC TGAAGCTGTA CTTCCGTGAG CTGCCCGAGC 
     4441 CCCTCTTCAC TGACGAGTTC TACCCCAACT TCGCAGAGGG CATCGCTCTT TCAGACCCGG 
     4501 TTGCAAAGGA GAGCTGCATG CTCAACCTGC TGCTGTCCCT GCCGGAGGCC AACCTGCTCA 
     4561 CCTTCCTTTT CCTTCTGGAC CACCTGAAAA GGGTGGCAGA GAAGGAGGCA GTCAATAAGA 
     4621 TGTCCCTGCA CAACCTCGCC ACGGTCTTTG GCCCCACGCT GCTCCGGCCC TCCGAGAAGG 
     4681 AGAGCAAGCT CCCTGCCAAC CCCAGCCAGC CTATCACCAT GACTGACAGC TGGTCCTTGG 
     4741 AGGTCATGTC CCAGGTCCAG GTGCTGCTGT ACTTCCTGCA GCTGGAGGCC ATCCCTGCCC 
     4801 CGGACAGCAA GAGACAGAGC ATCCTGTTCT CCACCGAAGT CACGCGTACG CGGCCGCTCG 
     4861 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4921 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4981 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5041 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5101 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5161 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5221 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5281 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5341 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5401 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5461 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5521 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5581 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5641 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5701 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5761 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5821 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5881 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5941 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6001 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6061 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6121 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6181 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6241 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6301 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6361 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6421 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6481 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6541 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6601 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6661 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6721 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6781 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6841 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6901 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6961 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7021 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7081 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7141 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7201 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7261 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7321 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7381 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7441 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7501 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7561 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7621 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7681 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7741 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7801 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7861 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7921 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8101 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8161 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8221 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8281 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8341 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8401 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8461 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8521 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8581 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8641 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//