LOCUS       RC211619                6747 bp    DNA     circular  SYN 
DEFINITION  CACNB2 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 5
ACCESSION   NM_201593
VERSION     NM_201593.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2904..2995 
                     /label="Myc-DDK"  
     misc_feature    3025..3610 
                     /label="PolyA signal"  
     misc_feature    3082..3101 
                     /label="Restriction Enzyme"  
     misc_feature    3886..4558 
                     /label="ColE1"  
     misc_feature    4988..5782 
                     /label="Kan/Neo"  
     misc_feature    5817..6119 
                     /label="SV40 ori"  
     misc_feature    6205..6682 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2886..2892 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2886 
                     /label="CACNB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCAAAGG GACATGTCCA AGTCGCCTCC CACAGCGGCG GCGGCGGTGG 
     1081 CGCAGGAGAT CCAGATGGAA CTGCTAGAGA ACGTGGCTCC CGCGGGGGCG CTCGGAGCCG 
     1141 CCGCACAGTC ATATGGAAAA GGAGCCAGAA GGAAAAACAG ATTTAAAGGA TCTGATGGAA 
     1201 GCACGTCATC TGATACTACC TCAAATAGTT TTGTTCGCCA GGGTTCGGCA GACTCCTACA 
     1261 CTAGCCGTCC ATCCGATTCC GATGTATCTC TGGAGGAGGA CCGGGAGGCA GTGCGCAGAG 
     1321 AAGCGGAGCG GCAGGCCCAG GCACAGTTGG AAAAAGCAAA GACAAAGCCC GTTGCATTTG 
     1381 CGGTTCGGAC AAATGTCAGC TACAGTGCGG CCCATGAAGA TGATGTTCCA GTGCCTGGCA 
     1441 TGGCCATCTC ATTCGAAGCA AAAGATTTTC TGCATGTTAA GGAAAAATTT AACAATGACT 
     1501 GGTGGATAGG GCGATTGGTA AAAGAAGGCT GTGAAATCGG ATTCATTCCA AGCCCAGTCA 
     1561 AACTAGAAAA CATGAGGCTG CAGCATGAAC AGAGAGCCAA GCAAGGGAAA TTCTACTCCA 
     1621 GTAAATCAGG AGGAAATTCA TCATCCAGTT TGGGTGACAT AGTACCTAGT TCCAGAAAAT 
     1681 CAACACCTCC ATCATCTGCT AAGCAGAAGC AGAAATCGAC AGAGCACACT CCTCCGTATG 
     1741 ATGTGGTACC TTCCATGCGA CCAGTGGTCC TAGTGGGCCC TTCTCTGAAG GGCTACGAGG 
     1801 TCACAGATAT GATGCAAAAA GCGCTGTTTG ATTTTTTAAA ACACAGATTT GAAGGGCGGA 
     1861 TATCCATCAC AAGGGTCACC GCTGACATCT CGCTTGCCAA ACGCTCGGTA TTAAACAATC 
     1921 CCAGTAAGCA CGCAATAATA GAAAGATCCA ACACAAGGTC AAGCTTAGCG GAAGTTCAGA 
     1981 GTGAAATCGA AAGGATTTTT GAACTTGCAA GAACATTGCA GTTGGTGGTC CTTGACGCGG 
     2041 ATACAATTAA TCATCCAGCT CAACTCAGTA AAACCTCCTT GGCCCCTATT ATAGTATATG 
     2101 TAAAGATTTC TTCTCCTAAG GTTTTACAAA GGTTAATAAA ATCTCGAGGG AAATCTCAAG 
     2161 CTAAACACCT CAACGTCCAG ATGGTAGCAG CTGATAAACT GGCTCAGTGT CCTCCAGAGC 
     2221 TGTTCGATGT GATCTTGGAT GAGAACCAGC TTGAGGATGC CTGTGAGCAC CTTGCCGACT 
     2281 ATCTGGAGGC CTACTGGAAG GCCACCCATC CTCCCAGCAG TAGCCTCCCC AACCCTCTCC 
     2341 TTAGCCGTAC ATTAGCCACT TCAAGTCTGC CTCTTAGCCC CACCCTAGCC TCTAATTCAC 
     2401 AGGGTTCTCA AGGTGATCAG AGGACTGATC GCTCCGCTCC TATCCGTTCT GCTTCCCAAG 
     2461 CTGAAGAAGA ACCTAGTGTG GAACCAGTCA AGAAATCCCA GCACCGCTCT TCCTCCTCAG 
     2521 CCCCACACCA CAACCATCGC AGTGGGACAA GTCGCGGCCT CTCCAGGCAA GAGACATTTG 
     2581 ACTCGGAAAC CCAGGAGAGT CGAGACTCTG CCTACGTAGA GCCAAAGGAA GATTATTCCC 
     2641 ATGACCACGT GGACCACTAT GCCTCACACC GTGACCACAA CCACAGAGAC GAGACCCACG 
     2701 GGAGCAGTGA CCACAGACAC AGGGAGTCCC GGCACCGTTC CCGGGACGTG GATCGAGAGC 
     2761 AGGACCACAA CGAGTGCAAC AAGCAGCGCA GCCGTCATAA ATCCAAGGAT CGCTACTGTG 
     2821 AAAAGGATGG AGAAGTGATA TCAAAAAAAC GGAATGAGGC TGGGGAGTGG AACAGGGATG 
     2881 TTTACATCCG CCAAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     2941 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3001 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3061 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3121 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3181 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3241 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3301 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3361 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3421 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3481 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3541 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3601 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3661 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3721 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3781 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     3841 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     3901 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     3961 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4021 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4081 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4141 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4201 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4261 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4321 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4381 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4441 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4501 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4561 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4621 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4681 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4741 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4801 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     4861 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     4921 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     4981 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5041 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5101 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5161 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5221 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5281 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5341 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5401 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5461 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5521 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5581 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5641 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5701 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5761 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     5821 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     5881 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     5941 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6001 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6061 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6121 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6181 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6301 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6361 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6421 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6481 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6541 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6601 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6661 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6721 ATTTAACAAA AATTTAACGC GAATTTT
//