LOCUS       RC211611                7458 bp    DNA     circular  SYN 
DEFINITION  MYRIP (Myc-DDK-tagged)-Human myosin VIIA and Rab interacting protein (MYRIP)
ACCESSION   NM_015460
VERSION     NM_015460.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3615..3706 
                     /label="Myc-DDK"  
     misc_feature    3736..4321 
                     /label="PolyA signal"  
     misc_feature    3793..3812 
                     /label="Restriction Enzyme"  
     misc_feature    4597..5269 
                     /label="ColE1"  
     misc_feature    5699..6493 
                     /label="Kan/Neo"  
     misc_feature    6528..6830 
                     /label="SV40 ori"  
     misc_feature    6916..7393 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3597..3603 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3597 
                     /label="MYRIP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAGGAAG CTGGACCTGT CTGGTTTGAC TGATGATGAA ACAGAGCATG 
     1081 TTCTTCAGGT GGTTCAAAGA GACTTCAATC TTCGCAAAAA AGAAGAAGAA CGACTAAGTG 
     1141 AGCTGAAGCA GAAGCTGGAT GAGGAAGGCA GCAAGTGCAG CATCCTCTCG AAGCACCAGC 
     1201 AGTTTGTGGA GCACTGCTGC ATGCGCTGCT GCTCGCCCTT CACCTTCCTC GTCAACACCA 
     1261 AGCGCCAGTG TGGAGATTGC AAATTCAATG TCTGCAAGAG CTGCTGCTCC TACCAGAAGC 
     1321 ACGAAAAGGC CTGGGTCTGC TGCGTCTGCC AGCAAGCGAG GCTTCTGAGG GCCCAATCTC 
     1381 TGGAATGGTT CTACAATAAT GTGAAGAGCC GCTTCAAGCG CTTTGGCAGT GCCAAGGTTC 
     1441 TGAAGAACCT GTACAGGAAG CACCGGCTGG AGAGTGGCGC GTGCTTCGAC ATTCTAGGAG 
     1501 GAAGCCTTTT TGAGTCAAAC CTGGAGAATG AAGGAAGCAT TTCTGGCAGT GATTCAACAT 
     1561 TTTATAGGCA GTCAGAAGGA CATAGTGTGA TGGACACCTT GGCTGTGGCC CTACGGGTGG 
     1621 CTGAAGAGGC CATTGAGGAA GCAATTTCCA AAGCAGAGGC ATATGGGGAC AGCCTGGACA 
     1681 AGCAAAATGA GGCCAGTTAC CTGCGGGACC ACAAGGAGGA GCTAACTGAG GAACTGGCCA 
     1741 CGACAATCCT GCAGAAGATT ATACGAAAAC AGAAGAGCAA AAGTGAGCAG CAAGTGGAAG 
     1801 AAGAGCCAGG ATGGCCACAT CCCCAGAGTT GCAGCACAAA GGTGGCAGAT GAGGGGACCT 
     1861 CAGCATCCCC TGGAGGCTAC CGTGCTCCCG CTGCCCTCTG GAGGTCCCAG TCTGCCTTCT 
     1921 CAATCACTGG AGAAGAAGCC CTGAAGACCC CTCCAGTGGA GGCTCCATCG AGGCAGCCAA 
     1981 GGGACCAAGG CCAACACCCG AGAGCAGAGT CTGCTCTGCC CAGCTGGAAG AGTGTGGACA 
     2041 GGCTGGATGA AACAAACCTG GCCCCAGTTT TGCAGAGCCC CGACGGGAAC TGGGTGGCCC 
     2101 TGAAGGATGG CGCTCCACCC CCCACCCGAC TACTGGCCAA ACCTAAGAGC GGGACGTTTC 
     2161 AGGCCCTGGA GGTGGCCTCC AGTGTGGCAT CTGCCTACGA TGAGATGGGC TCCGATAGCG 
     2221 AGGAAGACTT TGACTGGAGT GAGGCCTTGA GCAAGCTGTG TCCCAGGTCC CGGGCCCTGC 
     2281 CCAGGAACCC CCAGCCTCAG CCCACACAGG CCCAGAGCTC TGACCAAGGC CCCATAGCTG 
     2341 CCTCCCCATC CTCTGCACTC TCCCCCAACC CTGAGGCCAT GTGCTCTGAC TCGGAGACCT 
     2401 CCTCCGCAGG CTCTTCCCGA GAATTTGGGC ACCAGGCCAG ACTGTCCTGG TTGCAGAGGA 
     2461 AGGCCCCCAG GAACCCTGCA GCTGAGAAGA TGCGCTTGCA TGGAGAGCTG GACGTGAACT 
     2521 TCAACCCCCA GTTGGCCAGC AGGGAGACCT CGGACAGCAG CGAGCCGGAG GAGGCCCCCC 
     2581 ACACCACAGA CCGGCGGGCC AGGAGGTGGA GAAGAGCCCG ATTGGGCTCA GAAGAGCCAA 
     2641 GCAAAGAACC ATCTTCCCCC AGCGCCCAGC TCCGGGATCT AGACACACAT CAGGTGTCGG 
     2701 ATGATTTATC AGAGACAGAC ATCAGCAATG AGGCTCGGGA CCCCCAGACT CTCACAGACA 
     2761 CCACAGAGGA GAAACGGAGA AACAGGCTGT ACGAGTTAGC AATGAAAATG AGTGAAAAGG 
     2821 AGACTTCTTC AGGGGAGGAT CAGGAGTCTG AGCCCAAGAC AGAATCTGAG AACCAGAAGG 
     2881 AAAGTCTGTC CTCTGAAGAC AACAGCCAGA GTGTCCAGGA AGAGCTGAAG AAGAAGTTTT 
     2941 CTGCTGTTTC TCTCTGCAAC ATCTCCACAG AAGTCCTGAA AGTCATCAAT GCCACAGAGG 
     3001 AGTTGATAGC AGGATCTACA GGGCCCTGGG AGTCCCCACA AGTCCCTCCT GACAGACAGA 
     3061 AGGGGATGTT TCCTCGTGGG ACAGACCAAG TGAGACTGGA TGAGCAGCTG ACTTCCCTGG 
     3121 AAGAAAATGT ATACCTGGCA GCAGGCACTG TGTATGGACT GGAGACCCAG CTGACTGAGC 
     3181 TAGAAGATGC CGCCCGCTGC ATCCACAGTG GCACTGATGA GACCCATCTG GCGGATCTGG 
     3241 AGGACCAGGT GGCCACGGCT GCAGCCCAAG TCCACCATGC TGAACTCCAG ATTTCAGATA 
     3301 TTGAGAGCCG GATTTCAGCC CTGACCATTG CAGGATTAAA CATAGCACCA TGTGTGCGCT 
     3361 TCACAAGAAG ACGGGATCAG AAGCAAAGGA CCCAGGTACA AACCATAGAT ACATCAAGGC 
     3421 AGCAAAGGAG GAAACTGCCT GCTCCACCGG TGAAAGCTGA AAAAATTGAG ACATCTTCAG 
     3481 TGACTACCAT TAAAACATTT AACCACAACT TCATTCTCCA AGGCTCCTCA ACAAACAGGA 
     3541 CTAAGGAAAG GAAAGGCACC ACCAAGGATT TGATGGAGCC TGCTCTGGAG TCAGCTGTGA 
     3601 TGTACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3661 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3721 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3781 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3841 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3901 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3961 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4021 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4081 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4141 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4201 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4261 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4321 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4381 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4441 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4501 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4561 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4621 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4681 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4741 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4801 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4861 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4921 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4981 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5041 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5101 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5161 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5221 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5281 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5341 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5401 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5461 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5521 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5581 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5641 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5701 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5761 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5821 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5881 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5941 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6001 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6061 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6121 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6181 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6241 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6301 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6361 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6421 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6481 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6541 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6601 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6661 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6721 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6781 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6841 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6901 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6961 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7021 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7081 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7141 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7201 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7261 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7321 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7381 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7441 AAATTTAACG CGAATTTT
//