LOCUS       RC211601                8652 bp    DNA     circular  SYN 
DEFINITION  L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 1
ACCESSION   NM_000425
VERSION     NM_000425.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4809..4900 
                     /label="Myc-DDK"  
     misc_feature    4930..5515 
                     /label="PolyA signal"  
     misc_feature    4987..5006 
                     /label="Restriction Enzyme"  
     misc_feature    5791..6463 
                     /label="ColE1"  
     misc_feature    6893..7687 
                     /label="Kan/Neo"  
     misc_feature    7722..8024 
                     /label="SV40 ori"  
     misc_feature    8110..8587 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4791..4797 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4791 
                     /label="L1CAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCGTGGCG CTGCGGTACG TGTGGCCTCT CCTCCTCTGC AGCCCCTGCC 
     1081 TGCTTATCCA GATCCCCGAG GAATATGAAG GACACCATGT GATGGAGCCA CCTGTCATCA 
     1141 CGGAACAGTC TCCACGGCGC CTGGTTGTCT TCCCCACAGA TGACATCAGC CTCAAGTGTG 
     1201 AGGCCAGTGG CAAGCCCGAA GTGCAGTTCC GCTGGACGAG GGATGGTGTC CACTTCAAAC 
     1261 CCAAGGAAGA GCTGGGTGTG ACCGTGTACC AGTCGCCCCA CTCTGGCTCC TTCACCATCA 
     1321 CGGGCAACAA CAGCAACTTT GCTCAGAGGT TCCAGGGCAT CTACCGCTGC TTTGCCAGCA 
     1381 ATAAGCTGGG CACCGCCATG TCCCATGAGA TCCGGCTCAT GGCCGAGGGT GCCCCCAAGT 
     1441 GGCCAAAGGA GACAGTGAAG CCCGTGGAGG TGGAGGAAGG GGAGTCAGTG GTTCTGCCTT 
     1501 GCAACCCTCC CCCAAGTGCA GAGCCTCTCC GGATCTACTG GATGAACAGC AAGATCTTGC 
     1561 ACATCAAGCA GGACGAGCGG GTGACGATGG GCCAGAACGG CAACCTCTAC TTTGCCAATG 
     1621 TGCTCACCTC CGACAACCAC TCAGACTACA TCTGCCACGC CCACTTCCCA GGCACCAGGA 
     1681 CCATCATTCA GAAGGAACCC ATTGACCTCC GGGTCAAGGC CACCAACAGC ATGATTGACA 
     1741 GGAAGCCGCG CCTGCTCTTC CCCACCAACT CCAGCAGCCA CCTGGTGGCC TTGCAGGGGC 
     1801 AGCCATTGGT CCTGGAGTGC ATCGCCGAGG GCTTTCCCAC GCCCACCATC AAATGGCTGC 
     1861 GCCCCAGTGG CCCCATGCCA GCCGACCGTG TCACCTACCA GAACCACAAC AAGACCCTGC 
     1921 AGCTGCTGAA AGTGGGCGAG GAGGATGATG GCGAGTACCG CTGCCTGGCC GAGAACTCAC 
     1981 TGGGCAGTGC CCGGCATGCG TACTATGTCA CCGTGGAGGC TGCCCCGTAC TGGCTGCACA 
     2041 AGCCCCAGAG CCATCTATAT GGGCCAGGAG AGACTGCCCG CCTGGACTGC CAAGTCCAGG 
     2101 GCAGGCCCCA ACCAGAGGTC ACCTGGAGAA TCAACGGGAT CCCTGTGGAG GAGCTGGCCA 
     2161 AAGACCAGAA GTACCGGATT CAGCGTGGCG CCCTGATCCT GAGCAACGTG CAGCCCAGTG 
     2221 ACACAATGGT GACCCAATGT GAGGCCCGCA ACCGGCACGG GCTCTTGCTG GCCAATGCCT 
     2281 ACATCTACGT TGTCCAGCTG CCAGCCAAGA TCCTGACTGC GGACAATCAG ACGTACATGG 
     2341 CTGTCCAGGG CAGCACTGCC TACCTTCTGT GCAAGGCCTT CGGAGCGCCT GTGCCCAGTG 
     2401 TTCAGTGGCT GGACGAGGAT GGGACAACAG TGCTTCAGGA CGAACGCTTC TTCCCCTATG 
     2461 CCAATGGGAC CCTGGGCATT CGAGACCTCC AGGCCAATGA CACCGGACGC TACTTCTGCC 
     2521 TGGCTGCCAA TGACCAAAAC AATGTTACCA TCATGGCTAA CCTGAAGGTT AAAGATGCAA 
     2581 CTCAGATCAC TCAGGGGCCC CGCAGCACAA TCGAGAAGAA AGGTTCCAGG GTGACCTTCA 
     2641 CGTGCCAGGC CTCCTTTGAC CCCTCCTTGC AGCCCAGCAT CACCTGGCGT GGGGACGGTC 
     2701 GAGACCTCCA GGAGCTTGGG GACAGTGACA AGTACTTCAT AGAGGATGGG CGCCTGGTCA 
     2761 TCCACAGCCT GGACTACAGC GACCAGGGCA ACTACAGCTG CGTGGCCAGT ACCGAACTGG 
     2821 ATGTGGTGGA GAGTAGGGCA CAGCTCTTGG TGGTGGGGAG CCCTGGGCCG GTGCCACGGC 
     2881 TGGTGCTGTC CGACCTGCAC CTGCTGACGC AGAGCCAGGT GCGCGTGTCC TGGAGTCCTG 
     2941 CAGAAGACCA CAATGCCCCC ATTGAGAAAT ATGACATTGA ATTTGAGGAC AAGGAAATGG 
     3001 CGCCTGAAAA ATGGTACAGT CTGGGCAAGG TTCCAGGGAA CCAGACCTCT ACCACCCTCA 
     3061 AGCTGTCGCC CTATGTCCAC TACACCTTTA GGGTTACTGC CATAAACAAA TATGGCCCCG 
     3121 GGGAGCCCAG CCCGGTCTCT GAGACTGTGG TCACACCTGA GGCAGCCCCA GAGAAGAACC 
     3181 CTGTGGATGT GAAGGGGGAA GGAAATGAGA CCACCAATAT GGTCATCACG TGGAAGCCGC 
     3241 TCCGGTGGAT GGACTGGAAC GCCCCCCAGG TTCAGTACCG CGTGCAGTGG CGCCCTCAGG 
     3301 GGACACGAGG GCCCTGGCAG GAGCAGATTG TCAGCGACCC CTTCCTGGTG GTGTCCAACA 
     3361 CGTCCACCTT CGTGCCCTAT GAGATCAAAG TCCAGGCCGT CAACAGCCAG GGCAAGGGAC 
     3421 CAGAGCCCCA GGTCACTATC GGCTACTCTG GAGAGGACTA CCCCCAGGCA ATCCCTGAGC 
     3481 TGGAAGGCAT TGAAATCCTC AACTCAAGTG CCGTGCTGGT CAAGTGGCGG CCGGTGGACC 
     3541 TGGCCCAGGT CAAGGGCCAC CTCCGCGGAT ACAATGTGAC GTACTGGAGG GAGGGCAGTC 
     3601 AGAGGAAGCA CAGCAAGAGA CATATCCACA AAGACCATGT GGTGGTGCCC GCCAACACCA 
     3661 CCAGTGTCAT CCTCAGTGGC TTGCGGCCCT ATAGCTCCTA CCACCTGGAG GTGCAGGCCT 
     3721 TTAACGGGCG AGGATCGGGG CCCGCCAGCG AGTTCACCTT CAGCACCCCA GAGGGAGTGC 
     3781 CTGGCCACCC CGAGGCGTTG CACCTGGAGT GCCAGTCGAA CACCAGCCTG CTGCTGCGCT 
     3841 GGCAGCCCCC ACTCAGCCAC AACGGCGTGC TCACCGGCTA CGTGCTCTCC TACCACCCCC 
     3901 TGGATGAGGG GGGCAAGGGG CAACTGTCCT TCAACCTTCG GGACCCCGAA CTTCGGACAC 
     3961 ACAACCTGAC CGATCTCAGC CCCCACCTGC GGTACCGCTT CCAGCTTCAG GCCACCACCA 
     4021 AAGAGGGCCC TGGTGAAGCC ATCGTACGGG AAGGAGGCAC TATGGCCTTG TCTGGGATCT 
     4081 CAGATTTTGG CAACATCTCA GCCACAGCGG GTGAAAACTA CAGTGTCGTC TCCTGGGTCC 
     4141 CCAAGGAGGG CCAGTGCAAC TTCAGGTTCC ATATCTTGTT CAAAGCCTTG GGAGAAGAGA 
     4201 AGGGTGGGGC TTCCCTTTCG CCACAGTATG TCAGCTACAA CCAGAGCTCC TACACGCAGT 
     4261 GGGACCTGCA GCCTGACACT GACTACGAGA TCCACTTGTT TAAGGAGAGG ATGTTCCGGC 
     4321 ACCAAATGGC TGTGAAGACC AATGGCACAG GCCGCGTGAG GCTCCCTCCT GCTGGCTTCG 
     4381 CCACTGAGGG CTGGTTCATC GGCTTTGTGA GTGCCATCAT CCTCCTGCTC CTCGTCCTGC 
     4441 TCATCCTCTG CTTCATCAAG CGCAGCAAGG GCGGCAAATA CTCAGTGAAG GATAAGGAGG 
     4501 ACACCCAGGT GGACTCTGAG GCCCGACCGA TGAAAGATGA GACCTTCGGC GAGTACAGGT 
     4561 CCCTGGAGAG TGACAACGAG GAGAAGGCCT TTGGCAGCAG CCAGCCATCG CTCAACGGGG 
     4621 ACATCAAGCC CCTGGGCAGT GACGACAGCC TGGCCGATTA TGGGGGCAGC GTGGATGTTC 
     4681 AGTTCAACGA GGATGGTTCG TTCATTGGCC AGTACAGTGG CAAGAAGGAG AAGGAGGCGG 
     4741 CAGGGGGCAA TGACAGCTCA GGGGCCACTT CCCCCATCAA CCCTGCCGTG GCCCTAGAAA 
     4801 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4861 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4921 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4981 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5041 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5101 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5161 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5221 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5281 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5341 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5401 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5461 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5521 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5881 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6121 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6421 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6481 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6541 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6601 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6661 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6721 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6781 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6841 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6901 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6961 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7021 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7081 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7141 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7201 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7261 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7321 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7381 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7441 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7501 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7561 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7621 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7681 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7741 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7801 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7861 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8041 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8221 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8281 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8341 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8401 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8461 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8521 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8581 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8641 AACGCGAATT TT
//