LOCUS       RC211595                8331 bp    DNA     circular  SYN 
DEFINITION  PRTG (Myc-DDK-tagged)-Human protogenin (PRTG)
ACCESSION   NM_173814
VERSION     NM_173814.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="PRTG"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCTCCT CTGCGACCCC TCGCCCGGCT GCGACCGCCG GGGATGCTGC 
     1081 TCCGCGCGCT CCTGCTCCTG CTGCTGCTCA GTCCTTTGCC AGGAGTGTGG TGCTTTAGCG 
     1141 AACTGTCTTT TGTAAAAGAA CCACAGGATG TAACTGTCAC AAGAAAGGAC CCAGTCGTTT 
     1201 TAGATTGCCA GGCTCACGGA GAAGTTCCTA TTAAGGTCAC ATGGTTGAAA AATGGAGCAA 
     1261 AAATGTCTGA AAATAAACGG ATCGAGGTTC TTTCTAACGG CTCTTTATAC ATCAGTGAGG 
     1321 TGGAAGGCAG GCGAGGAGAG CAGTCCGATG AAGGATTTTA TCAGTGCTTG GCAATGAACA 
     1381 AATATGGAGC CATTCTTAGT CAAAAAGCTC ATCTTGCCTT ATCAACTATT TCTGCATTTG 
     1441 AAGTCCAGCC AATTTCCACT GAGGTCCACG AAGGTGGAGT TGCTCGATTT GCATGCAAGA 
     1501 TTTCATCCCA CCCTCCTGCA GTCATAACAT GGGAGTTCAA TCGGACAACT CTACCTATGA 
     1561 CTATGGACAG GATAACTGCC CTACCAACAG GAGTATTGCA GATCTATGAT GTCAGCCAAA 
     1621 GGGATTCTGG AAATTATCGT TGTATTGCTG CCACTGTAGC CCACCGACGT AAAAGTATGG 
     1681 AGGCCTCGCT AACTGTGATT CCAGCTAAGG AGTCAAAATC CTTCCACACA CCAACAATTA 
     1741 TAGCAGGTCC ACAGAACATA ACAACATCTC TTCATCAGAC TGTAGTTTTG GAATGCATGG 
     1801 CCACAGGAAA TCCCAAACCA ATCATTTCTT GGAGCCGCCT TGATCACAAA TCCATTGATG 
     1861 TCTTTAATAC TCGGGTACTT GGAAATGGTA ATCTCATGAT ATCTGATGTC AGGCTACAAC 
     1921 ATGCTGGAGT ATATGTTTGT CGGGCCACTA CCCCTGGCAC ACGCAACTTT ACAGTTGCTA 
     1981 TGGCAACTTT AACTGTATTA GCTCCTCCTT CATTTGTTGA ATGGCCAGAA AGTTTAACAA 
     2041 GGCCTCGAGC TGGCACTGCT CGATTTGTGT GTCAGGCAGA AGGAATCCCC TCTCCCAAGA 
     2101 TGTCATGGTT GAAAAATGGA AGGAAGATAC ATTCGAATGG TAGAATTAAA ATGTACAACA 
     2161 GTAAATTGGT AATTAACCAG ATTATTCCTG AAGATGATGC TATTTATCAG TGCATGGCTG 
     2221 AGAATAGCCA AGGATCTATT TTATCTAGAG CCAGACTGAC TGTAGTGATG TCAGAAGACA 
     2281 GACCCAGTGC TCCCTATAAT GTACATGCTG AAACCATGTC AAGCTCAGCC ATTCTTTTAG 
     2341 CCTGGGAGAG GCCACTTTAT AATTCAGACA AAGTCATTGC CTATTCTGTA CACTACATGA 
     2401 AAGCAGAAGG TTTAAATAAT GAAGAGTATC AAGTAGTCAT CGGAAATGAC ACAACTCATT 
     2461 ATATTATTGA TGACTTAGAG CCTGCCAGCA ATTATACTTT CTACATTGTA GCATATATGC 
     2521 CAATGGGAGC CAGCCAGATG TCTGACCATG TGACACAGAA TACTCTAGAG GATGTTCCCC 
     2581 TGAGACCTCC TGAAATTAGT TTGACAAGTC GAAGTCCCAC TGATATTCTC ATCTCCTGGC 
     2641 TGCCAATCCC AGCCAAATAT CGGCGGGGCC AAGTGGTGCT GTATCGCTTG TCTTTCCGCC 
     2701 TAAGTACTGA GAATTCAATC CAAGTTCTGG AGCTCCCGGG GACCACGCAT GAGTACCTTT 
     2761 TGGAAGGCCT GAAACCTGAC AGTGTCTACC TGGTTCGGAT TACTGCTGCC ACCAGAGTGG 
     2821 GGCTGGGAGA GTCATCAGTA TGGACTTCAC ATAGGACGCC CAAAGCTACA AGCGTGAAAG 
     2881 CCCCTAAGTC TCCAGAGTTG CATTTGGAGC CTCTGAACTG TACCACCATT TCTGTGAGGT 
     2941 GGCAGCAAGA TGTAGAGGAC ACAGCTGCTA TTCAGGGCTA CAAGCTGTAC TACAAGGAAG 
     3001 AAGGGCAGCA GGAGAATGGG CCCATTTTCT TGGATACCAA GGACCTACTC TATACTCTCA 
     3061 GTGGCTTAGA CCCCAGAAGA AAATATCATG TGAGACTCCT GGCTTACAAC AACATAGACG 
     3121 ATGGCTATCA GGCAGATCAG ACTGTCAGCA CTCCAGGATG CGTGTCTGTT CGTGATCGCA 
     3181 TGGTCCCTCC TCCACCACCA CCCCACCATC TCTATGCGAA GGCTAACACC TCATCTTCCA 
     3241 TCTTCCTGCA CTGGAGGAGG CCTGCATTCA CCGCTGCACA AATCATTAAC TACACCATCC 
     3301 GCTGTAATCC TGTTGGCCTG CAGAATGCTT CTTTGGTTCT GTACCTTCAA ACATCAGAAA 
     3361 CTCACATGTT GGTTCAAGGT CTAGAACCAA ACACCAAATA CGAATTTGCC GTTCGATTAC 
     3421 ATGTGGATCA GCTTTCCAGT CCTTGGAGCC CTGTAGTCTA CCATTCTACT CTTCCAGAAG 
     3481 CACCAGCAGG CCCACCAGTT GGAGTAAAAG TGACATTAAT AGAGGATGAC ACTGCCCTGG 
     3541 TTTCTTGGAA ACCCCCTGAT GGCCCAGAAA CAGTTGTGAC CCGCTATACT ATCTTATATG 
     3601 CATCTAGGAA GGCCTGGATT GCAGGAGAGT GGCAGGTCTT ACACCGTGAA GGGGCAATAA 
     3661 CCATGGCTTT GCTAGAAAAC TTGGTAGCAG GAAATGTGTA CATTGTCAAG ATATCTGCAT 
     3721 CCAATGAGGT GGGAGAAGGA CCCTTTTCAA ATTCTGTGGA GCTGGCAGTA CTTCCAAAGG 
     3781 AAACCTCTGA ATCAAATCAG AGGCCCAAGC GTTTAGATTC TGCTGATGCC AAAGTTTATT 
     3841 CAGGATATTA CCATCTGGAC CAAAAATCAA TGACTGGCAT TGCTGTAGGT GTTGGCATAG 
     3901 CCTTGACCTG CATCCTCATC TGTGTTCTCA TCTTGATATA CCGAAGTAAA GCCAGGAAAT 
     3961 CATCTGCTTC CAAGACGGCA CAGAATGGAA CTCAACAGTT ACCTCGTACC AGTGCCTCCT 
     4021 TAGCTAGTGG AAATGAGGTA GGAAAGAACC TGGAAGGAGC TGTAGGAAAT GAAGAATCTT 
     4081 TAATGCCAAT GATCATGCCA AACAGCTTCA TTGATGCAAA GGGAGGAACT GACCTGATAA 
     4141 TTAATAGCTA TGGTCCTATA ATTAAAAACA ACTCTAAGAA AAAGTGGTTT TTTTTCCAAG 
     4201 ACTCAAAGAA GATACAAGTT GAGCAGCCTC AAAGAAGATT TACTCCAGCG GTCTGCTTTT 
     4261 ACCAGCCAGG CACCACTGTA TTAATCAGTG ATGAAGACTC CCCTAGCTCC CCAGGTCAGA 
     4321 CAACCAGCTT CTCAAGACCC TTTGGTGTTG CAGCTGATAC AGAACATTCA GCAAATAGTG 
     4381 AAGGCAGCCA TGAGACTGGG GATTCTGGGC GGTTTTCTCA TGAGTCCAAC GATGAGATAC 
     4441 ATCTGTCCTC AGTTATAAGT ACCACACCCC CCAACCTCAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//