LOCUS       RC211593                8685 bp    DNA     circular  SYN 
DEFINITION  SEC24B (Myc-DDK-tagged)-Human SEC24 family, member B (S. cerevisiae) (SEC24B), transcript variant 1
ACCESSION   NM_006323
VERSION     NM_006323.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4842..4933 
                     /label="Myc-DDK"  
     misc_feature    4963..5548 
                     /label="PolyA signal"  
     misc_feature    5020..5039 
                     /label="Restriction Enzyme"  
     misc_feature    5824..6496 
                     /label="ColE1"  
     misc_feature    6926..7720 
                     /label="Kan/Neo"  
     misc_feature    7755..8057 
                     /label="SV40 ori"  
     misc_feature    8143..8620 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4824..4830 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4824 
                     /label="SEC24B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCCCC GCCGGGTCCT CTCACCCGGC CGCCAGCGCC CGGATCCCGC 
     1081 CCAAGTTCGG CGGATCGGCC GTCTCAGGAG CCGCAGCGCC CGCGGGCCCG GGTGCGGGCC 
     1141 CGGCGCCGCA CCAGCAGAAC GGTCCAGCCC AGAATCAAAT GCAGGTTCCA TCTGGATATG 
     1201 GATTGCATCA TCAAAACTAT ATTGCTCCCT CAGGACATTA CTCTCAAGGA CCTGGGAAAA 
     1261 TGACCTCATT GCCATTGGAT ACCCAGTGTG GTGATTACTA CTCTGCTCTC TATACAGTAC 
     1321 CAACACAAAA TGTGACTCCT AACACAGTGA ACCAGCAACC AGGAGCACAG CAGTTGTACA 
     1381 GCAGGGGTCC TCCTGCCCCT CATATTGTGG GATCCACTCT AGGATCTTTC CAAGGTGCTG 
     1441 CATCGTCAGC ATCCCATTTG CATACGAGTG CCTCCCAACC ATACTCCTCT TTTGTGAATC 
     1501 ACTACAATAG TCCAGCCATG TACTCTGCCA GCTCTTCTGT TGCGTCTCAG GGATTTCCCT 
     1561 CTACTTGTGG TCATTATGCT ATGTCAACTG TTTCTAATGC CGCGTATCCT AGTGTTTCAT 
     1621 ATCCCTCTCT GCCTGCTGGT GATACATATG GGCAAATGTT TACCTCACAG AATGCTCCGA 
     1681 CTGTTAGGCC AGTTAAAGAT AATTCATTCT CTGGTCAAAA TACAGCTATC AGCCATCCAT 
     1741 CGCCACTTCC ACCTCTACCA TCACAACAGC ACCACCAGCA GCAAAGTCTT TCAGGATACA 
     1801 GTACTCTAAC GTGGTCATCT CCAGGCCTTC CATCGACTCA AGACAATCTC ATCCGAAACC 
     1861 ACACAGGATC CCTGGCTGTA GCGAACAACA ACCCAACCAT TACTGTTGCA GATTCTTTAT 
     1921 CCTGTCCTGT TATGCAAAAT GTTCAGCCTC CCAAGTCCAG CCCAGTGGTA TCCACTGTTT 
     1981 TATCAGGATC CTCAGGATCC TCATCAACAA GAACACCTCC CACTGCAAAT CACCCAGTTG 
     2041 AGCCTGTGAC CTCAGTTACA CAGCCATCAG AGCTATTACA ACAAAAAGGC GTGCAGTATG 
     2101 GTGAATATGT TAATAACCAA GCTAGCTCCG CACCAACTCC CTTGTCATCA ACTTCCGATG 
     2161 ATGAGGAAGA GGAGGAGGAG GATGAGGAAG CAGGTGTTGA CAGCTCTTCT ACCACAAGCA 
     2221 GTGCTTCTCC AATGCCCAAC AGTTATGATG CCCTGGAAGG AGGCAGTTAC CCAGATATGC 
     2281 TTTCTTCATC AGCAAGCAGT CCTGCTCCTG ATCCCGCCCC TGAACCTGAT CCTGCTTCTG 
     2341 CTCCAGCTCC AGCTTCAGCT CCAGCTCCTG TCGTCCCTCA GCCTTCAAAA ATGGCTAAGC 
     2401 CTTTGGCTAT GGCTATCCAA CACTTCAGCC TGGTTATCAG AATGCTACAG CACCACTTAT 
     2461 TTCTGGAGTA CAGCCCCAGT AACCCGGTAT ATTCTGGATT CCAGCAGTAT CCTCAACAGT 
     2521 ATCCTGGTGT GAACCAGCTA TCCTCCAGTA TAGGAGGATT GAGTCTTCAG AGTTCTCCAC 
     2581 AACCAGAAAG CCTGAGACCT GTAAACCTTA CTCAGGAGAG GAATATTTTA CCTATGACTC 
     2641 CTGTTTGGGC TCCTGTACCT AACTTGAATG CAGACCTCAA AAAATTAAAC TGTAGCCCAG 
     2701 ATTCATTTCG GTGTACTTTG ACAAATATTC CACAGACACA GGCTTTACTG AATAAAGCTA 
     2761 AGCTTCCTTT AGGATTGTTG TTACATCCCT TCAGAGACCT AACGCAATTA CCAGTGATAA 
     2821 CATCAAATAC CATTGTGAGG TGCCGATCCT GTCGAACGTA TATTAACCCC TTTGTATCCT 
     2881 TCATTGATCA ACGTAGATGG AAATGCAATT TGTGCTATAG AGTAAACGAT GTTCCTGAAG 
     2941 AATTTATGTA TAACCCCCTT ACCCGATCTT ATGGAGAGCC TCATAAACGA CCAGAAGTTC 
     3001 AGAATTCAAC TGTGGAGTTC ATTGCTTCTT CAGATTACAT GCTGCGTCCT CCTCAACCTG 
     3061 CAGTTTACTT GTTTGTTTTA GATGTGTCTC ATAATGCAGT GGAAGCTGGA TATTTGACAA 
     3121 TTTTGTGCCA GTCACTCCTA GAAAATCTAG ACAAGCTTCC TGGAGATTCA CGAACAAGAA 
     3181 TAGGATTCAT GACCTTTGAT AGCACTATTC ATTTCTACAA TTTACAAGAA GGATTATCAC 
     3241 AGCCTCAAAT GTTGATTGTG TCTGATATAG ATGATGTTTT TCTACCTACA CCGGATAGTT 
     3301 TACTTGTGAA TCTATATGAA AGTAAAGAGC TTATAAAAGA CTTACTGAAT GCATTACCAA 
     3361 ACATGTTCAC CAATACAAGA GAAACACACA GTGCCCTTGG TCCTGCACTT CAGGCTGCCT 
     3421 TTAAATTAAT GTCTCCAACA GGTGGCCGTG TGTCTGTATT TCAGACACAG TTACCTTCCT 
     3481 TGGGTGCAGG ACTTCTGCAA TCCAGAGAAG ATCCTAATCA GAGATCAAGT ACAAAGGTGG 
     3541 TACAACATCT TGGCCCTGCA ACTGATTTTT ATAAGAAACT TGCATTAGAT TGCTCGGGAC 
     3601 AGCAAACTGC AGTGGATTTG TTCCTTTTAA GTTCACAGTA TTCTGATCTT GCTTCTCTAG 
     3661 CTTGCATGTC CAAGTATTCT GCAGGGTGCA TCTATTATTA TCCATCATTC CACTATACTC 
     3721 ACAATCCTTC ACAAGCAGAA AAGTTACAAA AAGACCTAAA ACGGTATCTC ACAAGAAAAA 
     3781 TTGGGTTTGA AGCTGTTATG AGAATAAGGT GTACTAAAGG TCTTTCAATG CACACTTTTC 
     3841 ACGGTAACTT CTTTGTCCGT TCTACTGATT TGTTATCCCT TGCCAACATC AATCCTGATG 
     3901 CTGGATTTGC GGTGCAGTTG TCAATTGAAG AAAGTTTAAC AGATACTTCC TTAGTATGTT 
     3961 TTCAAACAGC CCTATTATAT ACATCAAGCA AAGGTGAGCG GAGAATTAGA GTACATACAC 
     4021 TTTGTTTGCC AGTGGTAAGT TCACTAGCAG ATGTATATGC GGGAGTGGAT GTACAAGCTG 
     4081 CCATCTGCCT TCTGGCAAAC ATGGCTGTGG ATCGGTCCGT TTCATCAAGT CTGTCAGATG 
     4141 CAAGAGATGC CTTAGTGAAT GCTGTAGTGG ACTCATTGTC TGCATATGGC TCAACTGTCT 
     4201 CAAATTTACA GCACTCTGCA TTGATGGCGC CCAGCTCCCT CAAGTTGTTT CCTCTCTATG 
     4261 TTTTGGCCCT TCTCAAACAG AAAGCATTTA GAACGGGTAC AAGCACACGG CTGGATGATC 
     4321 GTGTATATGC CATGTGTCAG ATAAAGTCTC AGCCACTTGT TCATCTAATG AAAATGATTC 
     4381 ATCCCAACTT ATACAGGATA GACAGATTGA CAGATGAGGG TGCAGTACAT GTTAATGACA 
     4441 GGATTGTACC ACAGCCACCT CTTCAAAAAT TGTCTGCAGA GAAGCTGACA AGAGAAGGTG 
     4501 CTTTCCTTAT GGACTGTGGC TCTGTTTTTT ACATTTGGGT TGGGAAAGGC TGTGACAATA 
     4561 ACTTCATAGA GGATGTGCTT GGATATACTA ATTTTGCATC AATACCACAG AAAATGACAC 
     4621 ATCTTCCAGA GCTAGATACA CTTTCATCAG AAAGAGCCAG ATCCTTCATA ACTTGGCTTA 
     4681 GAGACAGCAG ACCATTAAGT CCAATCCTTC ACATAGTAAA AGATGAGAGT CCTGCCAAAG 
     4741 CAGAATTTTT TCAGCATTTG ATTGAAGACC GGACAGAGGC TGCATTTTCT TACTATGAAT 
     4801 TTTTGCTTCA TGTTCAGCAG CAGATTTGTA AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4861 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4921 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4981 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5041 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5101 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5161 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5221 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5281 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5341 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5401 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5461 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5521 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5581 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5641 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5701 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5761 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5821 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5881 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5941 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6001 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6061 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6121 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6181 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6241 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6301 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6361 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6421 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6481 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6541 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6601 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6661 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6721 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6781 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6841 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6901 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6961 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7021 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7081 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7141 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7201 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7261 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7321 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7381 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7441 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7501 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7561 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7621 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7681 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7741 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7801 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7861 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7921 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7981 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8101 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8161 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8221 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8281 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8341 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8401 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8461 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8521 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8581 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8641 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//