LOCUS       RC211582L2                9012 bp    DNA     circular  SYN 
DEFINITION  BTK (Myc-DDK-tagged)-Human Bruton agammaglobulinemia tyrosine kinase (BTK)
ACCESSION   NM_000061
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7303..7483 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8211..8870 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7531..8150 
                     /label="ColE1"  
     misc_feature    6178..6198 
                     /label="LR50"  
     misc_feature    5318..6043 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6187..6778 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5297..5303 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5297 
                     /label="BTK"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCGCAGTG ATTCTGGAGA GCATCTTTCT 
     3361 GAAGCGATCC CAACAGAAAA AGAAAACATC ACCTCTAAAC TTCAAGAAGC GCCTGTTTCT 
     3421 CTTGACCGTG CACAAACTCT CCTACTATGA GTATGACTTT GAACGTGGGA GAAGAGGCAG 
     3481 TAAGAAGGGT TCAATAGATG TTGAGAAGAT CACTTGTGTT GAAACAGTGG TTCCTGAAAA 
     3541 AAATCCTCCT CCAGAAAGAC AGATTCCGAG AAGAGGTGAA GAGTCCAGTG AAATGGAGCA 
     3601 AATTTCAATC ATTGAAAGGT TCCCTTATCC CTTCCAGGTT GTATATGATG AAGGGCCTCT 
     3661 CTACGTCTTC TCCCCAACTG AAGAACTAAG GAAGCGGTGG ATTCACCAGC TCAAAAACGT 
     3721 AATCCGGTAC AACAGTGATC TGGTTCAGAA ATATCACCCT TGCTTCTGGA TCGATGGGCA 
     3781 GTATCTCTGC TGCTCTCAGA CAGCCAAAAA TGCTATGGGC TGCCAAATTT TGGAGAACAG 
     3841 GAATGGAAGC TTAAAACCTG GGAGTTCTCA CCGGAAGACA AAAAAGCCTC TTCCCCCAAC 
     3901 GCCTGAGGAG GACCAGATCT TGAAAAAGCC ACTACCGCCT GAGCCAGCAG CAGCACCAGT 
     3961 CTCCACAAGT GAGCTGAAAA AGGTTGTGGC CCTTTATGAT TACATGCCAA TGAATGCAAA 
     4021 TGATCTACAG CTGCGGAAGG GTGATGAATA TTTTATCTTG GAGGAAAGCA ACTTACCATG 
     4081 GTGGAGAGCA CGAGATAAAA ATGGGCAGGA AGGCTACATT CCTAGTAACT ATGTCACTGA 
     4141 AGCAGAAGAC TCCATAGAAA TGTATGAGTG GTATTCCAAA CACATGACTC GGAGTCAGGC 
     4201 TGAGCAACTG CTAAAGCAAG AGGGGAAAGA AGGAGGTTTC ATTGTCAGAG ACTCCAGCAA 
     4261 AGCTGGCAAA TATACAGTGT CTGTGTTTGC TAAATCCACA GGGGACCCTC AAGGGGTGAT 
     4321 ACGTCATTAT GTTGTGTGTT CCACACCTCA GAGCCAGTAT TACCTGGCTG AGAAGCACCT 
     4381 TTTCAGCACC ATCCCTGAGC TCATTAACTA CCATCAGCAC AACTCTGCAG GACTCATATC 
     4441 CAGGCTCAAA TATCCAGTGT CTCAACAAAA CAAGAATGCA CCTTCCACTG CAGGCCTGGG 
     4501 ATACGGATCA TGGGAAATTG ATCCAAAGGA CCTGACCTTC TTGAAGGAGC TGGGGACTGG 
     4561 ACAATTTGGG GTAGTGAAGT ATGGGAAATG GAGAGGCCAG TACGACGTGG CCATCAAGAT 
     4621 GATCAAAGAA GGCTCCATGT CTGAAGATGA ATTCATTGAA GAAGCCAAAG TCATGATGAA 
     4681 TCTTTCCCAT GAGAAGCTGG TGCAGTTGTA TGGCGTCTGC ACCAAGCAGC GCCCCATCTT 
     4741 CATCATCACT GAGTACATGG CCAATGGCTG CCTCCTGAAC TACCTGAGGG AGATGCGCCA 
     4801 CCGCTTCCAG ACTCAGCAGC TGCTAGAGAT GTGCAAGGAT GTCTGTGAAG CCATGGAATA 
     4861 CCTGGAGTCA AAGCAGTTCC TTCACCGAGA CCTGGCAGCT CGAAACTGTT TGGTAAACGA 
     4921 TCAAGGAGTT GTTAAAGTAT CTGATTTCGG CCTGTCCAGG TATGTCCTGG ATGATGAATA 
     4981 CACAAGCTCA GTAGGCTCCA AATTTCCAGT CCGGTGGTCC CCACCGGAAG TCCTGATGTA 
     5041 TAGCAAGTTC AGCAGCAAAT CTGACATTTG GGCTTTTGGG GTTTTGATGT GGGAAATTTA 
     5101 CTCCCTGGGG AAGATGCCAT ATGAGAGATT TACTAACAGT GAGACTGCTG AACACATTGC 
     5161 CCAAGGCCTA CGTCTCTACA GGCCTCATCT GGCTTCAGAG AAGGTATATA CCATCATGTA 
     5221 CAGTTGCTGG CATGAGAAAG CAGATGAGCG TCCCACTTTC AAAATTCTTC TGAGCAATAT 
     5281 TCTAGATGTC ATGGATGAAG AATCCACGCG TACGCGGCCG CTCGAGATGA GCGGGGGCGA 
     5341 GGAGCTGTTC GCCGGCATCG TGCCCGTGCT GATCGAGCTG GACGGCGACG TGCACGGCCA 
     5401 CAAGTTCAGC GTGCGCGGCG AGGGCGAGGG CGACGCCGAC TACGGCAAGC TGGAGATCAA 
     5461 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTGGTGA CCACCCTCTG 
     5521 CTACGGCATC CAGTGCTTCG CCCGCTACCC CGAGCACATG AAGATGAACG ACTTCTTCAA 
     5581 GAGCGCCATG CCCGAGGGCT ACATCCAGGA GCGCACCATC CAGTTCCAGG ACGACGGCAA 
     5641 GTACAAGACC CGCGGCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT 
     5701 GAAGGGCAAG GACTTCAAGG AGGACGGCAA CATCCTGGGC CACAAGCTGG AGTACAGCTT 
     5761 CAACAGCCAC AACGTGTACA TCCGCCCCGA CAAGGCCAAC AACGGCCTGG AGGCTAACTT 
     5821 CAAGACCCGC CACAACATCG AGGGCGGCGG CGTGCAGCTG GCCGACCACT ACCAGACCAA 
     5881 CGTGCCCCTG GGCGACGGCC CCGTGCTGAT CCCCATCAAC CACTACCTGA GCACTCAGAC 
     5941 CAAGATCAGC AAGGACCGCA ACGAGGCCCG CGACCACATG GTGCTCCTGG AGTCCTTCAG 
     6001 CGCCTGCTGC CACACCCACG GCATGGACGA GCTGTACAGG TCCGGACTCA GATAAGTTTA 
     6061 AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG 
     6121 CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT 
     6181 ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 
     6241 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 
     6301 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 
     6361 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 
     6421 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 
     6481 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 
     6541 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 
     6601 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 
     6661 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 
     6721 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 
     6781 CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG 
     6841 CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT 
     6901 GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA 
     6961 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG 
     7021 ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA 
     7081 CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT 
     7141 ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA 
     7201 CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA 
     7261 CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT 
     7321 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA 
     7381 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC 
     7441 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG 
     7501 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     7561 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     7621 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     7681 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     7741 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     7801 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     7861 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     7921 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     7981 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     8041 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     8101 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     8161 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT 
     8221 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 
     8281 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 
     8341 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 
     8401 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC 
     8461 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 
     8521 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 
     8581 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 
     8641 CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 
     8701 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
     8761 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 
     8821 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA 
     8881 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8941 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     9001 GTGCCACCTG AC
//