LOCUS       RC211570                8172 bp    DNA     circular  SYN 
DEFINITION  REST (Myc-DDK-tagged)-Human RE1-silencing transcription factor (REST), transcript variant 1
ACCESSION   NM_005612
VERSION     NM_005612.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4329..4420 
                     /label="Myc-DDK"  
     misc_feature    4450..5035 
                     /label="PolyA signal"  
     misc_feature    4507..4526 
                     /label="Restriction Enzyme"  
     misc_feature    5311..5983 
                     /label="ColE1"  
     misc_feature    6413..7207 
                     /label="Kan/Neo"  
     misc_feature    7242..7544 
                     /label="SV40 ori"  
     misc_feature    7630..8107 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4311..4317 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4311 
                     /label="REST"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACCCAG GTAATGGGGC AGTCTTCTGG AGGAGGAGGG CTGTTTACCA 
     1081 GCAGTGGCAA CATTGGAATG GCCCTGCCTA ACGACATGTA TGACTTGCAT GACCTTTCCA 
     1141 AAGCTGAACT GGCCGCACCT CAGCTTATTA TGCTGGCAAA TGTGGCCTTA ACTGGGGAAG 
     1201 TAAATGGCAG CTGCTGTGAT TACCTGGTCG GTGAAGAAAG ACAGATGGCA GAACTGATGC 
     1261 CGGTTGGGGA TAACAACTTT TCAGATAGTG AAGAAGGAGA AGGACTTGAA GAGTCTGCTG 
     1321 ATATAAAAGG TGAACCTCAT GGACTGGAAA ACATGGAACT GAGAAGTTTG GAACTCAGCG 
     1381 TCGTAGAACC TCAGCCTGTA TTTGAGGCAT CAGGTGCTCC AGATATTTAC AGTTCAAATA 
     1441 AAGATCTTCC CCCTGAAACA CCTGGAGCGG AGGACAAAGG CAAGAGCTCG AAGACCAAAC 
     1501 CCTTTCGCTG TAAGCCATGC CAATATGAAG CAGAATCTGA AGAACAGTTT GTGCATCACA 
     1561 TCAGAGTTCA CAGTGCTAAG AAATTTTTTG TGGAAGAGAG TGCAGAGAAG CAGGCAAAAG 
     1621 CCAGGGAATC TGGCTCTTCC ACTGCAGAAG AGGGAGATTT CTCCAAGGGC CCCATTCGCT 
     1681 GTGACCGCTG CGGCTACAAT ACTAATCGAT ATGATCACTA TACAGCACAC CTGAAACACC 
     1741 ACACCAGAGC TGGGGATAAT GAGCGAGTCT ACAAGTGTAT CATTTGCACA TACACAACAG 
     1801 TGAGCGAGTA TCACTGGAGG AAACATTTAA GAAACCATTT TCCAAGGAAA GTATACACAT 
     1861 GTGGAAAATG CAACTATTTT TCAGACAGAA AAAACAATTA TGTTCAGCAT GTTAGAACTC 
     1921 ATACAGGAGA ACGCCCATAT AAATGTGAAC TTTGTCCTTA CTCAAGTTCT CAGAAGACTC 
     1981 ATCTAACTAG ACATATGCGT ACTCATTCAG GTGAGAAGCC ATTTAAATGT GATCAGTGCA 
     2041 GTTATGTGGC CTCTAATCAA CATGAAGTAA CCCGCCATGC AAGACAGGTT CACAATGGGC 
     2101 CTAAACCTCT TAATTGCCCA CACTGTGATT ACAAAACAGC AGATAGAAGC AACTTCAAAA 
     2161 AACATGTAGA GCTACATGTG AACCCACGGC AGTTCAATTG CCCTGTATGT GACTATGCAG 
     2221 CTTCCAAGAA GTGTAATCTA CAGTATCACT TCAAATCTAA GCATCCTACT TGTCCTAATA 
     2281 AAACAATGGA TGTCTCAAAA GTGAAACTAA AGAAAACCAA AAAACGAGAG GCTGACTTGC 
     2341 CTGATAATAT TACCAATGAA AAAACAGAAA TAGAACAAAC AAAAATAAAA GGGGATGTGG 
     2401 CTGGAAAGAA AAATGAAAAG TCCGTCAAAG CAGAGAAAAG AGATGTCTCA AAAGAGAAAA 
     2461 AGCCTTCTAA TAATGTGTCA GTGATCCAGG TGACTACCAG AACTCGAAAA TCAGTAACAG 
     2521 AGGTGAAAGA GATGGATGTG CATACAGGAA GCAATTCAGA AAAATTCAGT AAAACTAAGA 
     2581 AAAGCAAAAG GAAGCTGGAA GTTGACAGCC ATTCTTTACA TGGTCCTGTG AATGATGAGG 
     2641 AATCTTCAAC AAAAAAGAAA AAGAAGGTAG AAAGCAAATC CAAAAATAAT AGTCAGGAAG 
     2701 TGCCAAAGGG TGACAGCAAA GTGGAGGAGA ATAAAAAGCA AAATACTTGC ATGAAAAAAA 
     2761 GTACAAAGAA GAAAACTCTG AAAAATAAAT CAAGTAAGAA AAGCAGTAAG CCTCCTCAGA 
     2821 AGGAACCTGT TGAGAAGGGA TCTGCTCAGA TGGACCCTCC TCAGATGGGG CCTGCTCCCA 
     2881 CAGAGGCGGT TCAGAAGGGG CCCGTTCAGG TGGAGCCGCC ACCTCCCATG GAGCATGCTC 
     2941 AGATGGAGGG TGCCCAGATA CGGCCTGCTC CTGACGAGCC TGTTCAGATG GAGGTGGTTC 
     3001 AGGAGGGGCC TGCTCAGAAG GAGCTGCTGC CTCCCGTGGA GCCTGCTCAG ATGGTGGGTG 
     3061 CCCAAATTGT ACTTGCTCAC ATGGAGCTGC CTCCTCCCAT GGAGACTGCT CAGACGGAGG 
     3121 TTGCCCAAAT GGGGCCTGCT CCCATGGAAC CTGCTCAGAT GGAGGTTGCC CAGGTAGAAT 
     3181 CTGCTCCCAT GCAGGTGGTC CAGAAGGAGC CTGTTCAGAT GGAGCTGTCT CCTCCCATGG 
     3241 AGGTGGTCCA GAAGGAGCCT GTTCAGATAG AGCTGTCTCC TCCCATGGAG GTGGTCCAGA 
     3301 AGGAACCTGT TAAGATAGAG CTGTCTCCTC CCATAGAGGT GGTCCAGAAG GAGCCTGTTC 
     3361 AGATGGAGTT GTCTCCTCCC ATGGGGGTGG TTCAGAAGGA GCCTGCTCAG AGGGAGCTAC 
     3421 CTCCTCCCAG AGAGCCTCCC CTTCACATGG AGCCAATTTC CAAAAAGCCT CCTCTCCGAA 
     3481 AAGATAAAAA GGAAAAGTCT AACATGCAGA GTGAAAGGGC ACGGAAGGAG CAAGTCCTTA 
     3541 TTGAAGTTGG CTTAGTGCCT GTTAAAGATA GCTGGCTTCT AAAGGAAAGT GTAAGCACAG 
     3601 AGGATCTCTC ACCACCATCA CCACCACTGC CAAAGGAAAA TTTAAGAGAA GAGGCATCAG 
     3661 GAGACCAAAA ATTACTCAAC ACAGGTGAAG GAAATAAAGA AGCCCCTCTT CAGAAAGTAG 
     3721 GAGCAGAAGA GGCAGATGAG AGCCTACCTG GTCTTGCTGC TAATATCAAC GAATCTACCC 
     3781 ATATTTCATC CTCTGGACAA AACTTGAATA CGCCAGAGGG TGAAACTTTA AATGGTAAAC 
     3841 ATCAGACTGA CAGTATAGTT TGTGAAATGA AAATGGACAC TGATCAGAAC ACAAGAGAGA 
     3901 ATCTCACTGG TATAAATTCA ACAGTTGAAG AACCAGTTTC ACCAATGCTT CCCCCTTCAG 
     3961 CAGTAGAAGA ACGTGAAGCA GTGTCCAAAA CTGCACTGGC ATCACCTCCT GCTACAATGG 
     4021 CAGCAAATGA GTCTCAGGAA ATTGATGAAG ATGAAGGCAT CCACAGCCAT GAAGGAAGTG 
     4081 ACCTAAGTGA CAACATGTCA GAGGGTAGTG ATGATTCTGG ATTGCATGGG GCTCGGCCAG 
     4141 TTCCACAAGA ATCTAGCAGA AAAAATGCAA AGGAAGCCTT GGCAGTCAAA GCAGCTAAGG 
     4201 GAGATTTTGT TTGTATCTTC TGTGATCGTT CTTTCAGAAA GGGAAAAGAT TACAGCAAAC 
     4261 ACCTCAATCG CCATTTGGTT AATGTGTACT ATCTTGAAGA AGCAGCTCAA GGGCAGGAGA 
     4321 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4381 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4441 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4501 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4561 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4621 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4681 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4741 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4801 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4861 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4921 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4981 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5041 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5101 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5161 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5221 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5281 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5341 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5401 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5461 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5521 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5581 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5641 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5701 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5761 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5821 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5881 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5941 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6001 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6061 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6121 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6181 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6241 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6301 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6361 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6421 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6481 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6541 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6601 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6661 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6721 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6781 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6841 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6901 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6961 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7021 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7081 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7141 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7201 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7261 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7321 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7381 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7441 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7621 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7681 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7741 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7801 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7861 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7921 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7981 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8041 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8101 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8161 AACGCGAATT TT
//