LOCUS       RC211547                8943 bp    DNA     circular  SYN 
DEFINITION  ROCK1 (Myc-DDK-tagged)-Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1)
ACCESSION   NM_005406
VERSION     NM_005406.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5100..5191 
                     /label="Myc-DDK"  
     misc_feature    5221..5806 
                     /label="PolyA signal"  
     misc_feature    5278..5297 
                     /label="Restriction Enzyme"  
     misc_feature    6082..6754 
                     /label="ColE1"  
     misc_feature    7184..7978 
                     /label="Kan/Neo"  
     misc_feature    8013..8315 
                     /label="SV40 ori"  
     misc_feature    8401..8878 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5082..5088 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5082 
                     /label="ROCK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGACTGGG GACAGTTTTG AGACTCGATT TGAAAAAATG GACAACCTGC 
     1081 TGCGGGATCC CAAATCGGAA GTGAATTCGG ATTGTTTGCT GGATGGATTG GATGCTTTGG 
     1141 TATATGATTT GGATTTTCCT GCCTTAAGAA AAAACAAAAA TATTGACAAC TTTTTAAGCA 
     1201 GATATAAAGA CACAATAAAT AAAATCAGAG ATTTACGAAT GAAAGCTGAA GATTATGAAG 
     1261 TAGTGAAGGT GATTGGTAGA GGTGCATTTG GAGAAGTTCA ATTGGTAAGG CATAAATCCA 
     1321 CCAGGAAGGT ATATGCTATG AAGCTTCTCA GCAAATTTGA AATGATAAAG AGATCTGATT 
     1381 CTGCTTTTTT CTGGGAAGAA AGGGACATCA TGGCTTTTGC CAACAGTCCT TGGGTTGTTC 
     1441 AGCTTTTTTA TGCATTCCAA GATGATCGTT ATCTCTACAT GGTGATGGAA TACATGCCTG 
     1501 GTGGAGATCT TGTAAACTTA ATGAGCAACT ATGATGTGCC TGAAAAATGG GCACGATTCT 
     1561 ATACTGCAGA AGTAGTTCTT GCATTGGATG CAATCCATTC CATGGGTTTT ATTCACAGAG 
     1621 ATGTGAAGCC TGATAACATG CTGCTGGATA AATCTGGACA TTTGAAGTTA GCAGATTTTG 
     1681 GTACTTGTAT GAAGATGAAT AAGGAAGGCA TGGTACGATG TGATACAGCG GTTGGAACAC 
     1741 CTGATTATAT TTCCCCTGAA GTATTAAAAT CCCAAGGTGG TGATGGTTAT TATGGAAGAG 
     1801 AATGTGACTG GTGGTCGGTT GGGGTATTTT TATACGAAAT GCTTGTAGGT GATACACCTT 
     1861 TTTATGCAGA TTCTTTGGTT GGAACTTACA GTAAAATTAT GAACCATAAA AATTCACTTA 
     1921 CCTTTCCTGA TGATAATGAC ATATCAAAAG AAGCAAAAAA CCTTATTTGT GCCTTCCTTA 
     1981 CTGACAGGGA AGTGAGGTTA GGGCGAAATG GTGTAGAAGA AATCAAACGA CATCTCTTCT 
     2041 TCAAAAATGA CCAGTGGGCT TGGGAAACGC TCCGAGACAC TGTAGCACCA GTTGTACCCG 
     2101 ATTTAAGTAG TGACATTGAT ACTAGTAATT TTGATGACTT GGAAGAAGAT AAAGGAGAGG 
     2161 AAGAAACATT CCCTATTCCT AAAGCTTTCG TTGGCAATCA ACTACCTTTT GTAGGATTTA 
     2221 CATATTATAG CAATCGTAGA TACTTATCTT CAGCAAATCC TAATGATAAC AGAACTAGCT 
     2281 CCAATGCAGA TAAAAGCTTG CAGGAAAGTT TGCAAAAAAC AATCTATAAG CTGGAAGAAC 
     2341 AGCTGCATAA TGAAATGCAG TTAAAAGATG AAATGGAGCA GAAGTGCAGA ACCTCAAACA 
     2401 TAAAACTAGA CAAGATAATG AAAGAATTGG ATGAAGAGGG AAATCAAAGA AGAAATCTAG 
     2461 AATCTACAGT GTCTCAGATT GAGAAGGAGA AAATGTTGCT ACAGCATAGA ATTAATGAGT 
     2521 ACCAAAGAAA AGCTGAACAG GAAAATGAGA AGAGAAGAAA TGTAGAAAAT GAAGTTTCTA 
     2581 CATTAAAGGA TCAGTTGGAA GACTTAAAGA AAGTCAGTCA GAATTCACAG CTTGCTAATG 
     2641 AGAAGCTGTC CCAGTTACAA AAGCAGCTAG AAGAAGCCAA TGACTTACTT AGGACAGAAT 
     2701 CGGACACAGC TGTAAGATTG AGGAAGAGTC ACACAGAGAT GAGCAAGTCA ATTAGTCAGT 
     2761 TAGAGTCCCT GAACAGAGAG TTGCAAGAGA GAAATCGAAT TTTAGAGAAT TCTAAGTCAC 
     2821 AAACAGACAA AGATTATTAC CAGCTGCAAG CTATATTAGA AGCTGAACGA AGAGACAGAG 
     2881 GTCATGATTC TGAGATGATT GGAGACCTTC AAGCTCGAAT TACATCTTTA CAAGAGGAGG 
     2941 TGAAGCATCT CAAACATAAT CTCGAAAAAG TGGAAGGAGA AAGAAAAGAG GCTCAAGACA 
     3001 TGCTTAATCA CTCAGAAAAG GAAAAGAATA ATTTAGAGAT AGATTTAAAC TACAAACTTA 
     3061 AATCATTACA ACAACGGTTA GAACAAGAGG TAAATGAACA CAAAGTAACC AAAGCTCGTT 
     3121 TAACTGACAA ACATCAATCT ATTGAAGAGG CAAAGTCTGT GGCAATGTGT GAGATGGAAA 
     3181 AAAAGCTGAA AGAAGAAAGA GAAGCTCGAG AGAAGGCTGA AAATCGGGTT GTTCAGATTG 
     3241 AGAAACAGTG TTCCATGCTA GACGTTGATC TGAAGCAATC TCAGCAGAAA CTAGAACATT 
     3301 TGACTGGAAA TAAAGAAAGG ATGGAGGATG AAGTTAAGAA TCTAACCCTG CAACTGGAGC 
     3361 AGGAATCAAA TAAGCGGCTG TTGTTACAAA ATGAATTGAA GACTCAAGCA TTTGAGGCAG 
     3421 ACAATTTAAA AGGTTTAGAA AAGCAGATGA AACAGGAAAT AAATACTTTA TTGGAAGCAA 
     3481 AGAGATTATT AGAATTTGAG TTAGCTCAGC TTACGAAACA GTATAGAGGA AATGAAGGAC 
     3541 AGATGCGGGA GCTACAAGAT CAGCTTGAAG CTGAGCAATA TTTCTCGACA CTTTATAAAA 
     3601 CCCAGGTAAA GGAACTTAAA GAAGAAATTG AAGAAAAAAA CAGAGAAAAT TTAAAGAAAA 
     3661 TACAGGAACT ACAAAATGAA AAAGAAACTC TTGCTACTCA GTTGGATCTA GCAGAAACAA 
     3721 AAGCTGAGTC TGAGCAGTTG GCGCGAGGCC TTCTGGAAGA ACAGTATTTT GAATTGACGC 
     3781 AAGAAAGCAA GAAAGCTGCT TCAAGAAATA GACAAGAGAT TACAGATAAA GATCACACTG 
     3841 TTAGTCGGCT TGAAGAAGCA AACAGCATGC TAACCAAAGA TATTGAAATA TTAAGAAGAG 
     3901 AGAATGAAGA GCTAACAGAG AAAATGAAGA AGGCAGAGGA AGAATATAAA CTGGAGAAGG 
     3961 AGGAGGAGAT CAGTAATCTT AAGGCTGCCT TTGAAAAGAA TATCAACACT GAACGAACCC 
     4021 TTAAAACACA GGCTGTTAAC AAATTGGCAG AAATAATGAA TCGAAAAGAT TTTAAAATTG 
     4081 ATAGAAAGAA AGCTAATACA CAAGATTTGA GAAAGAAAGA AAAGGAAAAT CGAAAGCTGC 
     4141 AACTGGAACT CAACCAAGAA AGAGAGAAAT TCAACCAGAT GGTAGTGAAA CATCAGAAGG 
     4201 AACTGAATGA CATGCAAGCG CAATTGGTAG AAGAATGTGC ACATAGGAAT GAGCTTCAGA 
     4261 TGCAGTTGGC CAGCAAAGAG AGTGATATTG AGCAATTGCG TGCTAAACTT TTGGACCTCT 
     4321 CGGATTCTAC AAGTGTTGCT AGTTTTCCTA GTGCTGATGA AACTGATGGT AACCTCCCAG 
     4381 AGTCAAGAAT TGAAGGTTGG CTTTCAGTAC CAAATAGAGG AAATATCAAA CGATATGGCT 
     4441 GGAAGAAACA GTATGTTGTG GTAAGCAGCA AAAAAATTTT GTTCTATAAT GACGAACAAG 
     4501 ATAAGGAGCA ATCCAATCCA TCTATGGTAT TGGACATAGA TAAACTGTTT CACGTTAGAC 
     4561 CTGTAACCCA AGGAGATGTG TATAGAGCTG AAACTGAAGA AATTCCTAAA ATATTCCAGA 
     4621 TACTATATGC AAATGAAGGT GAATGTAGAA AAGATGTAGA GATGGAACCA GTACAACAAG 
     4681 CTGAAAAAAC TAATTTCCAA AATCACAAAG GCCATGAGTT TATTCCTACA CTCTACCACT 
     4741 TTCCTGCCAA TTGTGATGCC TGTGCCAAAC CTCTCTGGCA TGTTTTTAAG CCACCCCCTG 
     4801 CCCTAGAGTG TCGAAGATGC CATGTTAAGT GCCACAGAGA TCACTTAGAT AAGAAAGAGG 
     4861 ACTTAATTTG TCCATGTAAA GTAAGTTATG ATGTAACATC AGCAAGAGAT ATGCTGCTGT 
     4921 TAGCATGTTC TCAGGATGAA CAAAAAAAAT GGGTAACTCA TTTAGTAAAG AAAATCCCTA 
     4981 AGAATCCACC ATCTGGTTTT GTTCGTGCTT CCCCTCGAAC GCTTTCTACA AGATCCACTG 
     5041 CAAATCAGTC TTTCCGGAAA GTGGTCAAAA ATACATCTGG AAAAACTAGT ACGCGTACGC 
     5101 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5161 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5221 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5281 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5341 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5401 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5461 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5521 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5581 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5641 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5701 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5761 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5821 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5881 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5941 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6001 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6061 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6121 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6181 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6241 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6301 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6361 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6421 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6481 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6541 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6601 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6661 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6721 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6781 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6841 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6901 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6961 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7021 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7081 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7141 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7201 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7261 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7321 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7381 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7441 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7501 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7561 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7621 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7681 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7741 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7801 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7861 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7921 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7981 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8041 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8101 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8161 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8221 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8281 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8341 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8401 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8461 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8521 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8581 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8641 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8701 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8761 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8821 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8881 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8941 TTT
//