LOCUS       RC211508                8280 bp    DNA     circular  SYN 
DEFINITION  DMD (Myc-DDK-tagged)-Human dystrophin (DMD), transcript variant Dp140bc
ACCESSION   NM_004023
VERSION     NM_004023.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4437..4528 
                     /label="Myc-DDK"  
     misc_feature    4558..5143 
                     /label="PolyA signal"  
     misc_feature    4615..4634 
                     /label="Restriction Enzyme"  
     misc_feature    5419..6091 
                     /label="ColE1"  
     misc_feature    6521..7315 
                     /label="Kan/Neo"  
     misc_feature    7350..7652 
                     /label="SV40 ori"  
     misc_feature    7738..8215 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4425 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCTTCC TTGATGTTGG AGGTACCTGC TCTGGCAGAT TTCAACCGGG 
     1081 CTTGGACAGA ACTTACCGAC TGGCTTTCTC TGCTTGATCA AGTTATAAAA TCACAGAGGG 
     1141 TGATGGTGGG TGACCTTGAG GATATCAACG AGATGATCAT CAAGCAGAAG GCAACAATGC 
     1201 AGGATTTGGA ACAGAGGCGT CCCCAGTTGG AAGAACTCAT TACCGCTGCC CAAAATTTGA 
     1261 AAAACAAGAC CAGCAATCAA GAGGCTAGAA CAATCATTAC GGATCGAATT GAAAGAATTC 
     1321 AGAATCAGTG GGATGAAGTA CAAGAACACC TTCAGAACCG GAGGCAACAG TTGAATGAAA 
     1381 TGTTAAAGGA TTCAACACAA TGGCTGGAAG CTAAGGAAGA AGCTGAGCAG GTCTTAGGAC 
     1441 AGGCCAGAGC CAAGCTTGAG TCATGGAAGG AGGGTCCCTA TACAGTAGAT GCAATCCAAA 
     1501 AGAAAATCAC AGAAACCAAG CAGTTGGCCA AAGACCTCCG CCAGTGGCAG ACAAATGTAG 
     1561 ATGTGGCAAA TGACTTGGCC CTGAAACTTC TCCGGGATTA TTCTGCAGAT GATACCAGAA 
     1621 AAGTCCACAT GATAACAGAG AATATCAATG CCTCTTGGAG AAGCATTCAT AAAAGGGTGA 
     1681 GTGAGCGAGA GGCTGCTTTG GAAGAAACTC ATAGATTACT GCAACAGTTC CCCCTGGACC 
     1741 TGGAAAAGTT TCTTGCCTGG CTTACAGAAG CTGAAACAAC TGCCAATGTC CTACAGGATG 
     1801 CTACCCGTAA GGAAAGGCTC CTAGAAGACT CCAAGGGAGT AAAAGAGCTG ATGAAACAAT 
     1861 GGCAAGACCT CCAAGGTGAA ATTGAAGCTC ACACAGATGT TTATCACAAC CTGGATGAAA 
     1921 ACAGCCAAAA AATCCTGAGA TCCCTGGAAG GTTCCGATGA TGCAGTCCTG TTACAAAGAC 
     1981 GTTTGGATAA CATGAACTTC AAGTGGAGTG AACTTCGGAA AAAGTCTCTC AACATTAGGT 
     2041 CCCATTTGGA AGCCAGTTCT GACCAGTGGA AGCGTCTGCA CCTTTCTCTG CAGGAACTTC 
     2101 TGGTGTGGCT ACAGCTGAAA GATGATGAAT TAAGCCGGCA GGCACCTATT GGAGGCGACT 
     2161 TTCCAGCAGT TCAGAAGCAG AACGATGTAC ATAGGGCCTT CAAGAGGGAA TTGAAAACTA 
     2221 AAGAACCTGT AATCATGAGT ACTCTTGAGA CTGTACGAAT ATTTCTGACA GAGCAGCCTT 
     2281 TGGAAGGACT AGAGAAACTC TACCAGGAGC CCAGAGAGCT GCCTCCTGAG GAGAGAGCCC 
     2341 AGAATGTCAC TCGGCTTCTA CGAAAGCAGG CTGAGGAGGT CAATACTGAG TGGGAAAAAT 
     2401 TGAACCTGCA CTCCGCTGAC TGGCAGAGAA AAATAGATGA GACCCTTGAA AGACTCCAGG 
     2461 AACTTCAAGA GGCCACGGAT GAGCTGGACC TCAAGCTGCG CCAAGCTGAG GTGATCAAGG 
     2521 GATCCTGGCA GCCCGTGGGC GATCTCCTCA TTGACTCTCT CCAAGATCAC CTCGAGAAAG 
     2581 TCAAGGCACT TCGAGGAGAA ATTGCGCCTC TGAAAGAGAA CGTGAGCCAC GTCAATGACC 
     2641 TTGCTCGCCA GCTTACCACT TTGGGCATTC AGCTCTCACC GTATAACCTC AGCACTCTGG 
     2701 AAGACCTGAA CACCAGATGG AAGCTTCTGC AGGTGGCCGT CGAGGACCGA GTCAGGCAGC 
     2761 TGCATGAAGC CCACAGGGAC TTTGGTCCAG CATCTCAGCA CTTTCTTTCC ACGTCTGTCC 
     2821 AGGGTCCCTG GGAGAGAGCC ATCTCGCCAA ACAAAGTGCC CTACTATATC AACCACGAGA 
     2881 CTCAAACAAC TTGCTGGGAC CATCCCAAAA TGACAGAGCT CTACCAGTCT TTAGCTGACC 
     2941 TGAATAATGT CAGATTCTCA GCTTATAGGA CTGCCATGAA ACTCCGAAGA CTGCAGAAGG 
     3001 CCCTTTGCTT GGATCTCTTG AGCCTGTCAG CTGCATGTGA TGCCTTGGAC CAGCACAACC 
     3061 TCAAGCAAAA TGACCAGCCC ATGGATATCC TGCAGATTAT TAATTGTTTG ACCACTATTT 
     3121 ATGACCGCCT GGAGCAAGAG CACAACAATT TGGTCAACGT CCCTCTCTGC GTGGATATGT 
     3181 GTCTGAACTG GCTGCTGAAT GTTTATGATA CGGGACGAAC AGGGAGGATC CGTGTCCTGT 
     3241 CTTTTAAAAC TGGCATCATT TCCCTGTGTA AAGCACATTT GGAAGACAAG TACAGATACC 
     3301 TTTTCAAGCA AGTGGCAAGT TCAACAGGAT TTTGTGACCA GCGCAGGCTG GGCCTCCTTC 
     3361 TGCATGATTC TATCCAAATT CCAAGACAGT TGGGTGAAGT TGCATCCTTT GGGGGCAGTA 
     3421 ACATTGAGCC AAGTGTCCGG AGCTGCTTCC AATTTGCTAA TAATAAGCCA GAGATCGAAG 
     3481 CGGCCCTCTT CCTAGACTGG ATGAGACTGG AACCCCAGTC CATGGTGTGG CTGCCCGTCC 
     3541 TGCACAGAGT GGCTGCTGCA GAAACTGCCA AGCATCAGGC CAAATGTAAC ATCTGCAAAG 
     3601 AGTGTCCAAT CATTGGATTC AGGTACAGGA GTCTAAAGCA CTTTAATTAT GACATCTGCC 
     3661 AAAGCTGCTT TTTTTCTGGT CGAGTTGCAA AAGGCCATAA AATGCACTAT CCCATGGTGG 
     3721 AATATTGCAC TCCGACTACA TCAGGAGAAG ATGTTCGAGA CTTTGCCAAG GTACTAAAAA 
     3781 ACAAATTTCG AACCAAAAGG TATTTTGCGA AGCATCCCCG AATGGGCTAC CTGCCAGTGC 
     3841 AGACTGTCTT AGAGGGGGAC AACATGGAAA CGAATCTGCA AGCAGAATAT GACCGTCTAA 
     3901 AGCAGCAGCA CGAACATAAA GGCCTGTCCC CACTGCCGTC CCCTCCTGAA ATGATGCCCA 
     3961 CCTCTCCCCA GAGTCCCCGG GATGCTGAGC TCATTGCTGA GGCCAAGCTA CTGCGTCAAC 
     4021 ACAAAGGCCG CCTGGAAGCC AGGATGCAAA TCCTGGAAGA CCACAATAAA CAGCTGGAGT 
     4081 CACAGTTACA CAGGCTAAGG CAGCTGCTGG AGCAACCCCA GGCAGAGGCC AAAGTGAATG 
     4141 GCACAACGGT GTCCTCTCCT TCTACCTCTC TACAGAGGTC CGACAGCAGT CAGCCTATGC 
     4201 TGCTCCGAGT GGTTGGCAGT CAAACTTCGG ACTCCATGGG TGAGGAAGAT CTTCTCAGTC 
     4261 CTCCCCAGGA CACAAGCACA GGGTTAGAGG AGGTGATGGA GCAACTCAAC AACTCCTTCC 
     4321 CTAGTTCAAG AGGACACAAT GTAGGAAGTC TTTTCCACAT GGCAGATGAT TTGGGCAGAG 
     4381 CGATGGAGTC CTTAGTATCA GTCATGACAG ATGAAGAAGG AGCAGAAACG CGTACGCGGC 
     4441 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4501 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4561 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4621 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4681 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4741 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4801 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4861 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4921 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4981 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5041 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5101 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5161 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5221 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5281 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5341 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5461 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5521 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5581 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5641 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5761 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5821 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5881 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5941 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6061 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6121 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6181 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6241 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6301 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6361 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6421 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6481 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6541 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6601 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6661 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6721 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6781 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6841 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6901 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6961 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7021 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7081 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7141 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7201 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7261 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7321 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7381 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7441 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7501 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7681 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7741 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7801 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7861 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7921 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7981 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8041 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8101 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8161 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8221 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//