LOCUS       RC211478L1                9288 bp    DNA     circular  SYN 
DEFINITION  CSE1L (Myc-DDK-tagged)-Human CSE1 chromosome segregation 1-like (yeast) (CSE1L)
ACCESSION   NM_001316
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7579..7759 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8487..9146 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7918..8486 
                     /label="ColE1"  
     misc_feature    6454..6474 
                     /label="LR50 primer"  
     misc_feature    6251..6322 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6463..7054 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6233..6239 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6233 
                     /label="CSE1L"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAACTCAGC GATGCAAATC TGCAAACACT 
     3361 AACAGAATAT TTAAAGAAAA CACTTGATCC TGATCCTGCC ATCCGACGTC CAGCTGAGAA 
     3421 ATTTCTTGAA TCTGTTGAAG GAAATCAGAA TTATCCACTG TTGCTTTTGA CATTACTGGA 
     3481 GAAGTCCCAG GATAATGTTA TCAAAGTATG TGCTTCAGTA ACATTCAAAA ACTATATTAA 
     3541 AAGGAACTGG AGAATTGTTG AAGATGAACC AAACAAAATT TGTGAAGCCG ATCGAGTGGC 
     3601 CATTAAAGCC AACATAGTGC ACTTGATGCT TAGCAGCCCA GAGCAAATTC AGAAGCAGTT 
     3661 AAGTGATGCA ATTAGCATTA TTGGCAGAGA AGATTTTCCA CAGAAATGGC CTGACTTGCT 
     3721 GACAGAAATG GTGAATCGCT TTCAGAGTGG AGATTTCCAT GTTATTAATG GAGTCCTCCG 
     3781 TACAGCACAT TCATTATTTA AAAGATACCG TCATGAATTT AAGTCAAACG AGTTATGGAC 
     3841 TGAAATTAAG CTTGTTCTGG ATGCCTTTGC TTTGCCTTTG ACTAATCTTT TTAAGGCCAC 
     3901 TATTGAACTC TGCAGTACCC ATGCAAATGA TGCCTCTGCC CTGAGGATTC TGTTTTCTTC 
     3961 CCTGATCCTG ATCTCAAAAT TGTTCTATAG TTTAAACTTT CAGGATCTCC CTGAATTTTT 
     4021 TGAAGATAAT ATGGAAACTT GGATGAATAA TTTTCATACT CTCTTAACAT TGGATAATAA 
     4081 GCTTTTACAA ACTGATGATG AAGAGGAAGC CGGCTTATTG GAGCTCTTAA AATCCCAGAT 
     4141 TTGTGATAAT GCCGCACTCT ATGCACAAAA GTACGATGAA GAATTCCAGC GATACCTGCC 
     4201 TCGTTTTGTT ACAGCCATCT GGAATTTACT AGTTACAACG GGTCAAGAGG TTAAATATGA 
     4261 TTTGTTGGTA AGTAATGCAA TTCAATTTCT GGCTTCAGTT TGTGAGAGAC CTCATTATAA 
     4321 GAATCTATTT GAGGACCAGA ACACGCTGAC AAGTATCTGT GAAAAGGTTA TTGTGCCTAA 
     4381 CATGGAATTT AGAGCTGCTG ATGAAGAAGC ATTTGAAGAT AATTCTGAGG AGTACATAAG 
     4441 GAGAGATTTG GAAGGATCTG ATATTGATAC TAGACGCAGG GCTGCTTGTG ATCTGGTACG 
     4501 AGGATTATGC AAGTTTTTTG AGGGACCTGT GACAGGAATC TTCTCTGGTT ATGTTAATTC 
     4561 CATGCTGCAG GAATACGCAA AAAATCCATC TGTCAACTGG AAACACAAAG ATGCAGCCAT 
     4621 CTACCTAGTG ACATCTTTGG CATCAAAAGC CCAAACACAG AAGCATGGAA TTACACAAGC 
     4681 AAATGAACTT GTAAACCTAA CTGAGTTCTT TGTGAATCAC ATCCTCCCTG ATTTAAAATC 
     4741 AGCTAATGTG AATGAATTTC CTGTCCTTAA AGCTGACGGT ATCAAATATA TTATGATTTT 
     4801 TAGAAATCAA GTGCCAAAAG AACATCTTTT AGTCTCGATT CCTCTCTTGA TTAATCATCT 
     4861 TCAAGCTGAA AGTATTGTTG TTCATACTTA CGCAGCTCAT GCTCTTGAAC GGCTCTTTAC 
     4921 TATGCGAGGG CCTAACAATG CCACTCTCTT TACAGCTGCA GAAATCGCAC CGTTTGTTGA 
     4981 GATTCTGCTA ACAAACCTTT TCAAAGCTCT CACACTTCCT GGCTCTTCAG AAAATGAATA 
     5041 TATTATGAAA GCTATCATGA GAAGTTTTTC TCTCCTACAA GAAGCCATAA TCCCCTACAT 
     5101 CCCTACTCTC ATCACTCAGC TTACACAGAA GCTATTAGCT GTTAGTAAGA ACCCAAGCAA 
     5161 ACCTCACTTT AATCACTACA TGTTTGAAGC AATATGTTTA TCCATAAGAA TAACTTGCAA 
     5221 AGCTAACCCT GCTGCTGTTG TAAATTTTGA GGAGGCTTTG TTTTTGGTGT TTACTGAAAT 
     5281 CTTACAAAAT GATGTGCAAG AATTTATTCC ATACGTCTTT CAAGTGATGT CTTTGCTTCT 
     5341 GGAAACACAC AAAAATGACA TCCCGTCTTC CTATATGGCC TTATTTCCTC ATCTCCTTCA 
     5401 GCCAGTGCTT TGGGAAAGAA CAGGAAATAT TCCTGCTCTA GTGAGGCTTC TTCAAGCATT 
     5461 CTTAGAACGC GGTTCAAACA CAATAGCAAG TGCTGCAGCT GACAAAATTC CTGGGTTACT 
     5521 AGGTGTCTTT CAGAAGCTGA TTGCATCCAA AGCAAATGAC CACCAAGGTT TTTATCTTCT 
     5581 AAACAGTATA ATAGAGCACA TGCCTCCTGA ATCAGTTGAC CAATATAGGA AACAAATCTT 
     5641 CATTCTGCTA TTCCAGAGAC TTCAGAATTC CAAAACAACC AAGTTTATCA AGAGTTTTTT 
     5701 AGTCTTTATT AATTTGTATT GCATAAAATA TGGGGCACTA GCACTACAAG AAATATTTGA 
     5761 TGGTATACAA CCAAAAATGT TTGGAATGGT TTTGGAAAAA ATTATTATTC CTGAAATTCA 
     5821 GAAGGTATCT GGAAATGTAG AGAAAAAGAT CTGTGCGGTT GGCATAACCA AATTACTAAC 
     5881 AGAATGTCCC CCAATGATGG ACACTGAGTA TACCAAACTG TGGACTCCAT TATTACAGTC 
     5941 TTTGATTGGT CTTTTTGAGT TACCCGAAGA TGATACCATT CCTGATGAGG AACATTTTAT 
     6001 TGACATAGAA GATACACCAG GATATCAGAC TGCCTTCTCA CAGTTGGCAT TTGCTGGGAA 
     6061 AAAAGAGCAT GATCCTGTAG GTCAAATGGT GAATAACCCC AAAATTCACC TGGCACAGTC 
     6121 ACTTCACAAG TTGTCTACCG CCTGTCCAGG AAGGGTTCCA TCAATGGTGA GCACCAGCCT 
     6181 GAATGCAGAA GCGCTCCAGT ATCTCCAAGG GTACCTTCAG GCAGCCAGTG TGACACTGCT 
     6241 TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     6301 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     6361 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     6421 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     6481 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     6541 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     6601 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6661 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     6721 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     6781 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     6841 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     6901 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     6961 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     7021 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     7081 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     7141 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     7201 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     7261 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     7321 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     7381 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     7441 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     7501 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     7561 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     7621 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7681 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     7741 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     7801 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     7861 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     7921 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     7981 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     8041 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     8101 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     8161 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     8221 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     8281 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     8341 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     8401 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     8461 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     8521 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     8581 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     8641 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     8701 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     8761 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     8821 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     8881 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     8941 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     9001 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     9061 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     9121 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     9181 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9241 AAACAAATAG GGGTCCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//