LOCUS       RC211478                7794 bp    DNA     circular  SYN 
DEFINITION  CSE1L (Myc-DDK-tagged)-Human CSE1 chromosome segregation 1-like (yeast) (CSE1L)
ACCESSION   NM_001316
VERSION     NM_001316.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4042 
                     /label="Myc-DDK"  
     misc_feature    4072..4657 
                     /label="PolyA signal"  
     misc_feature    4129..4148 
                     /label="Restriction Enzyme"  
     misc_feature    4933..5605 
                     /label="ColE1"  
     misc_feature    6035..6829 
                     /label="Kan/Neo"  
     misc_feature    6864..7166 
                     /label="SV40 ori"  
     misc_feature    7252..7729 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3933..3939 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3933 
                     /label="CSE1L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACTCAGC GATGCAAATC TGCAAACACT AACAGAATAT TTAAAGAAAA 
     1081 CACTTGATCC TGATCCTGCC ATCCGACGTC CAGCTGAGAA ATTTCTTGAA TCTGTTGAAG 
     1141 GAAATCAGAA TTATCCACTG TTGCTTTTGA CATTACTGGA GAAGTCCCAG GATAATGTTA 
     1201 TCAAAGTATG TGCTTCAGTA ACATTCAAAA ACTATATTAA AAGGAACTGG AGAATTGTTG 
     1261 AAGATGAACC AAACAAAATT TGTGAAGCCG ATCGAGTGGC CATTAAAGCC AACATAGTGC 
     1321 ACTTGATGCT TAGCAGCCCA GAGCAAATTC AGAAGCAGTT AAGTGATGCA ATTAGCATTA 
     1381 TTGGCAGAGA AGATTTTCCA CAGAAATGGC CTGACTTGCT GACAGAAATG GTGAATCGCT 
     1441 TTCAGAGTGG AGATTTCCAT GTTATTAATG GAGTCCTCCG TACAGCACAT TCATTATTTA 
     1501 AAAGATACCG TCATGAATTT AAGTCAAACG AGTTATGGAC TGAAATTAAG CTTGTTCTGG 
     1561 ATGCCTTTGC TTTGCCTTTG ACTAATCTTT TTAAGGCCAC TATTGAACTC TGCAGTACCC 
     1621 ATGCAAATGA TGCCTCTGCC CTGAGGATTC TGTTTTCTTC CCTGATCCTG ATCTCAAAAT 
     1681 TGTTCTATAG TTTAAACTTT CAGGATCTCC CTGAATTTTT TGAAGATAAT ATGGAAACTT 
     1741 GGATGAATAA TTTTCATACT CTCTTAACAT TGGATAATAA GCTTTTACAA ACTGATGATG 
     1801 AAGAGGAAGC CGGCTTATTG GAGCTCTTAA AATCCCAGAT TTGTGATAAT GCCGCACTCT 
     1861 ATGCACAAAA GTACGATGAA GAATTCCAGC GATACCTGCC TCGTTTTGTT ACAGCCATCT 
     1921 GGAATTTACT AGTTACAACG GGTCAAGAGG TTAAATATGA TTTGTTGGTA AGTAATGCAA 
     1981 TTCAATTTCT GGCTTCAGTT TGTGAGAGAC CTCATTATAA GAATCTATTT GAGGACCAGA 
     2041 ACACGCTGAC AAGTATCTGT GAAAAGGTTA TTGTGCCTAA CATGGAATTT AGAGCTGCTG 
     2101 ATGAAGAAGC ATTTGAAGAT AATTCTGAGG AGTACATAAG GAGAGATTTG GAAGGATCTG 
     2161 ATATTGATAC TAGACGCAGG GCTGCTTGTG ATCTGGTACG AGGATTATGC AAGTTTTTTG 
     2221 AGGGACCTGT GACAGGAATC TTCTCTGGTT ATGTTAATTC CATGCTGCAG GAATACGCAA 
     2281 AAAATCCATC TGTCAACTGG AAACACAAAG ATGCAGCCAT CTACCTAGTG ACATCTTTGG 
     2341 CATCAAAAGC CCAAACACAG AAGCATGGAA TTACACAAGC AAATGAACTT GTAAACCTAA 
     2401 CTGAGTTCTT TGTGAATCAC ATCCTCCCTG ATTTAAAATC AGCTAATGTG AATGAATTTC 
     2461 CTGTCCTTAA AGCTGACGGT ATCAAATATA TTATGATTTT TAGAAATCAA GTGCCAAAAG 
     2521 AACATCTTTT AGTCTCGATT CCTCTCTTGA TTAATCATCT TCAAGCTGAA AGTATTGTTG 
     2581 TTCATACTTA CGCAGCTCAT GCTCTTGAAC GGCTCTTTAC TATGCGAGGG CCTAACAATG 
     2641 CCACTCTCTT TACAGCTGCA GAAATCGCAC CGTTTGTTGA GATTCTGCTA ACAAACCTTT 
     2701 TCAAAGCTCT CACACTTCCT GGCTCTTCAG AAAATGAATA TATTATGAAA GCTATCATGA 
     2761 GAAGTTTTTC TCTCCTACAA GAAGCCATAA TCCCCTACAT CCCTACTCTC ATCACTCAGC 
     2821 TTACACAGAA GCTATTAGCT GTTAGTAAGA ACCCAAGCAA ACCTCACTTT AATCACTACA 
     2881 TGTTTGAAGC AATATGTTTA TCCATAAGAA TAACTTGCAA AGCTAACCCT GCTGCTGTTG 
     2941 TAAATTTTGA GGAGGCTTTG TTTTTGGTGT TTACTGAAAT CTTACAAAAT GATGTGCAAG 
     3001 AATTTATTCC ATACGTCTTT CAAGTGATGT CTTTGCTTCT GGAAACACAC AAAAATGACA 
     3061 TCCCGTCTTC CTATATGGCC TTATTTCCTC ATCTCCTTCA GCCAGTGCTT TGGGAAAGAA 
     3121 CAGGAAATAT TCCTGCTCTA GTGAGGCTTC TTCAAGCATT CTTAGAACGC GGTTCAAACA 
     3181 CAATAGCAAG TGCTGCAGCT GACAAAATTC CTGGGTTACT AGGTGTCTTT CAGAAGCTGA 
     3241 TTGCATCCAA AGCAAATGAC CACCAAGGTT TTTATCTTCT AAACAGTATA ATAGAGCACA 
     3301 TGCCTCCTGA ATCAGTTGAC CAATATAGGA AACAAATCTT CATTCTGCTA TTCCAGAGAC 
     3361 TTCAGAATTC CAAAACAACC AAGTTTATCA AGAGTTTTTT AGTCTTTATT AATTTGTATT 
     3421 GCATAAAATA TGGGGCACTA GCACTACAAG AAATATTTGA TGGTATACAA CCAAAAATGT 
     3481 TTGGAATGGT TTTGGAAAAA ATTATTATTC CTGAAATTCA GAAGGTATCT GGAAATGTAG 
     3541 AGAAAAAGAT CTGTGCGGTT GGCATAACCA AATTACTAAC AGAATGTCCC CCAATGATGG 
     3601 ACACTGAGTA TACCAAACTG TGGACTCCAT TATTACAGTC TTTGATTGGT CTTTTTGAGT 
     3661 TACCCGAAGA TGATACCATT CCTGATGAGG AACATTTTAT TGACATAGAA GATACACCAG 
     3721 GATATCAGAC TGCCTTCTCA CAGTTGGCAT TTGCTGGGAA AAAAGAGCAT GATCCTGTAG 
     3781 GTCAAATGGT GAATAACCCC AAAATTCACC TGGCACAGTC ACTTCACAAG TTGTCTACCG 
     3841 CCTGTCCAGG AAGGGTTCCA TCAATGGTGA GCACCAGCCT GAATGCAGAA GCGCTCCAGT 
     3901 ATCTCCAAGG GTACCTTCAG GCAGCCAGTG TGACACTGCT TACGCGTACG CGGCCGCTCG 
     3961 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4021 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4081 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4141 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4201 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4261 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4321 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4381 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4441 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4501 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4561 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4621 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4681 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4741 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4801 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5461 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5521 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5581 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5641 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5701 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5761 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5821 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5881 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5941 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6001 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6061 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6121 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6181 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6241 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6301 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6361 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6421 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6481 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6541 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6601 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6661 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6721 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6781 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6841 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6901 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6961 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7021 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7141 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7201 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7261 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7321 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7381 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7441 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7501 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7561 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7621 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7681 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7741 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//