LOCUS       RC211460                7833 bp    DNA     circular  SYN 
DEFINITION  OPA1 (Myc-DDK-tagged)-Human optic atrophy 1 (autosomal dominant) (OPA1), nuclear gene encoding mitochondrial protein, transcript variant 5
ACCESSION   NM_130834
VERSION     NM_130834.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3988..4079 
                     /label="Myc-DDK"  
     misc_feature    4109..4694 
                     /label="PolyA signal"  
     misc_feature    4166..4185 
                     /label="Restriction Enzyme"  
     misc_feature    4970..5642 
                     /label="ColE1"  
     misc_feature    6072..6866 
                     /label="Kan/Neo"  
     misc_feature    6901..7203 
                     /label="SV40 ori"  
     misc_feature    7289..7766 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3970 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="OPA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGACTA CGTCGGGCCG CTGTGGCCTG TGAGGTCTGC CAGTCTTTAG 
     1081 TGAAACACAG CTCTGGAATA AAAGGAAGTT TACCACTACA AAAACTACAT CTGGTTTCAC 
     1141 GAAGCATTTA TCATTCACAT CATCCTACCT TAAAGCTTCA ACGACCCCAA TTAAGGACAT 
     1201 CCTTTCAGCA GTTCTCTTCT CTGACAAACC TTCCTTTACG TAAACTGAAA TTCTCTCCAA 
     1261 TTAAATATGG CTACCAGCCT CGCAGGAATT TTTGGCCAGC AAGATTAGCT ACGAGACTCT 
     1321 TAAAACTTCG CTATCTCATA CTAGGATCGG CTGTTGGGGG TGGCTACACA GCCAAAAAGA 
     1381 CTTTTGATCA GTGGAAAGAT ATGATACCGG ACCTTAGTGA ATATAAATGG ATTGTGCCTG 
     1441 ACATTGTGTG GGAAATTGAT GAGTATATCG ATTTTGAGAA AATTAGAAAA GCCCTTCCTA 
     1501 GTTCAGAAGA CCTTGTAAAG TTAGCACCAG ACTTTGACAA GATTGTTGAA AGCCTTAGCT 
     1561 TATTGAAGGA CTTTTTTACC TCAGGTCACA AATTGGTTAG TGAAGTCATA GGAGCTTCTG 
     1621 ACCTACTTCT CTTGTTAGGT TCTCCGGAAG AAACGGCGTT TAGAGCAACA GATCGTGGAT 
     1681 CTGAAAGTGA CAAGCATTTT AGAAAGGTGT CAGACAAAGA GAAAATTGAC CAACTTCAGG 
     1741 AAGAACTTCT GCACACTCAG TTGAAGTATC AGAGAATCTT GGAACGATTA GAAAAGGAGA 
     1801 ACAAAGAATT GAGAAAATTA GTATTGCAGA AAGATGACAA AGGCATTCAT CATAGAAAGC 
     1861 TTAAGAAATC TTTGATTGAC ATGTATTCTG AAGTTCTTGA TGTTCTCTCT GATTATGATG 
     1921 CCAGTTATAA TACGCAAGAT CATCTGCCAC GGGTTGTTGT GGTTGGAGAT CAGAGTGCTG 
     1981 GAAAGACTAG TGTGTTGGAA ATGATTGCCC AAGCTCGAAT ATTCCCAAGA GGATCTGGGG 
     2041 AGATGATGAC ACGTTCTCCA GTTAAGGTGA CTCTGAGTGA AGGTCCTCAC CATGTGGCCC 
     2101 TATTTAAAGA TAGTTCTCGG GAGTTTGATC TTACCAAAGA AGAAGATCTT GCAGCATTAA 
     2161 GACATGAAAT AGAACTTCGA ATGAGGAAAA ATGTGAAAGA AGGCTGTACC GTTAGCCCTG 
     2221 AGACCATATC CTTAAATGTA AAAGGCCCTG GACTACAGAG GATGGTGCTT GTTGACTTAC 
     2281 CAGGTGTGAT TAATACTGTG ACATCAGGCA TGGCTCCTGA CACAAAGGAA ACTATTTTCA 
     2341 GTATCAGCAA AGCTTACATG CAGAATCCTA ATGCCATCAT ACTGTGTATT CAAGATGGAT 
     2401 CTGTGGATGC TGAACGCAGT ATTGTTACAG ACTTGGTCAG TCAAATGGAC CCTCATGGAA 
     2461 GGAGAACCAT ATTCGTTTTG ACCAAAGTAG ACCTGGCAGA GAAAAATGTA GCCAGTCCAA 
     2521 GCAGGATTCA GCAGATAATT GAAGGAAAGC TCTTCCCAAT GAAAGCTTTA GGTTATTTTG 
     2581 CTGTTGTAAC AGGAAAAGGG AACAGCTCTG AAAGCATTGA AGCTATAAGA GAATATGAAG 
     2641 AAGAGTTTTT TCAGAATTCA AAGCTCCTAA AGACAAGCAT GCTAAAGGCA CACCAAGTGA 
     2701 CTACAAGAAA TTTAAGCCTT GCAGTATCAG ACTGCTTTTG GAAAATGGTA CGAGAGTCTG 
     2761 TTGAACAACA GGCTGATAGT TTCAAAGCAA CACGTTTTAA CCTTGAAACT GAATGGAAGA 
     2821 ATAACTATCC TCGCCTGCGG GAACTTGACC GGAATGAACT ATTTGAAAAA GCTAAAAATG 
     2881 AAATCCTTGA TGAAGTTATC AGTCTGAGCC AGGTTACACC AAAACATTGG GAGGAAATCC 
     2941 TTCAACAATC TTTGTGGGAA AGAGTATCAA CTCATGTGAT TGAAAACATC TACCTTCCAG 
     3001 CTGCGCAGAC CATGAATTCA GGAACTTTTA ACACCACAGT GGATATCAAG CTTAAACAGT 
     3061 GGACTGATAA ACAACTTCCT AATAAAGCAG TAGAGGTTGC TTGGGAGACC CTACAAGAAG 
     3121 AATTTTCCCG CTTTATGACA GAACCGAAAG GGAAAGAGCA TGATGACATA TTTGATAAAC 
     3181 TTAAAGAGGC TGTTAAGGAA GAAAGTATTA AACGACACAA GTGGAATGAC TTTGCGGAGG 
     3241 ACAGCTTGAG GGTTATTCAA CACAATGCTT TGGAAGACCG ATCCATATCT GATAAACAGC 
     3301 AATGGGATGC AGCTATTTAT TTTATGGAAG AGGCTCTGCA GGCTCGTCTC AAGGATACTG 
     3361 AAAATGCAAT TGAAAACATG GTGGGTCCAG ACTGGAAAAA GAGGTGGTTA TACTGGAAGA 
     3421 ATCGGACCCA AGAACAGTGT GTTCACAATG AAACCAAGAA TGAATTGGAG AAGATGTTGA 
     3481 AATGTAATGA GGAGCACCCA GCTTATCTTG CAAGTGATGA AATAACCACA GTCCGGAAGA 
     3541 ACCTTGAATC CCGAGGAGTA GAAGTAGATC CAAGCTTGAT TAAGGATACT TGGCATCAAG 
     3601 TTTATAGAAG ACATTTTTTA AAAACAGCTC TAAACCATTG TAACCTTTGT CGAAGAGGTT 
     3661 TTTATTACTA CCAAAGGCAT TTTGTAGATT CTGAGTTGGA ATGCAATGAT GTGGTCTTGT 
     3721 TTTGGCGTAT ACAGCGCATG CTTGCTATCA CCGCAAATAC TTTAAGGCAA CAACTTACAA 
     3781 ATACTGAAGT TAGGCGATTA GAGAAAAATG TTAAAGAGGT ATTGGAAGAT TTTGCTGAAG 
     3841 ATGGTGAGAA GAAGATTAAA TTGCTTACTG GTAAACGCGT TCAACTGGCG GAAGACCTCA 
     3901 AGAAAGTTAG AGAAATTCAA GAAAAACTTG ATGCTTTCAT TGAAGCTCTT CATCAGGAGA 
     3961 AAAGCGGACC GAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4021 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4081 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4141 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4201 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4261 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4321 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4381 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4441 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4501 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4561 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4621 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4681 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4741 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4801 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4861 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4921 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4981 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5041 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5101 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5161 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5221 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5281 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5341 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5401 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5461 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5521 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5581 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5641 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5701 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5761 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5821 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5881 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5941 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6001 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6061 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6121 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6181 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6241 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6301 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6361 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6421 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6481 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6541 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6601 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6661 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6721 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6781 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6841 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6901 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6961 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7021 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7081 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7141 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7201 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7261 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7321 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7381 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7441 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7501 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7561 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7621 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7681 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7741 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7801 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//