LOCUS       RC211459                7860 bp    DNA     circular  SYN 
DEFINITION  FLT3 (Myc-DDK-tagged)-Human fms-related tyrosine kinase 3 (FLT3)
ACCESSION   NM_004119
VERSION     NM_004119.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4017..4108 
                     /label="Myc-DDK"  
     misc_feature    4138..4723 
                     /label="PolyA signal"  
     misc_feature    4195..4214 
                     /label="Restriction Enzyme"  
     misc_feature    4999..5671 
                     /label="ColE1"  
     misc_feature    6101..6895 
                     /label="Kan/Neo"  
     misc_feature    6930..7232 
                     /label="SV40 ori"  
     misc_feature    7318..7795 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3999..4005 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3999 
                     /label="FLT3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCGTTG GCGCGCGGCG GCGGCCAGCT GCCGCTGCTC GTTGTTTTTT 
     1081 CTGCAATGAT ATTTGGGACT ATTACAAATC AAGATCTGCC TGTGATCAAG TGTGTTTTAA 
     1141 TCAATCATAA GAACAATGAT TCATCAGTGG GGAAGTCATC ATCATATCCC ATGGTATCAG 
     1201 AATCCCCGGA AGACCTCGGG TGTGCGTTGA GACCCCAGAG CTCAGGGACA GTGTACGAAG 
     1261 CTGCCGCTGT GGAAGTGGAT GTATCTGCTT CCATCACACT GCAAGTGCTG GTCGATGCCC 
     1321 CAGGGAACAT TTCCTGTCTC TGGGTCTTTA AGCACAGCTC CCTGAATTGC CAGCCACATT 
     1381 TTGATTTACA AAACAGAGGA GTTGTTTCCA TGGTCATTTT GAAAATGACA GAAACCCAAG 
     1441 CTGGAGAATA CCTACTTTTT ATTCAGAGTG AAGCTACCAA TTACACAATA TTGTTTACAG 
     1501 TGAGTATAAG AAATACCCTG CTTTACACAT TAAGAAGACC TTACTTTAGA AAAATGGAAA 
     1561 ACCAGGACGC CCTGGTCTGC ATATCTGAGA GCGTTCCAGA GCCGATCGTG GAATGGGTGC 
     1621 TTTGCGATTC ACAGGGGGAA AGCTGTAAAG AAGAAAGTCC AGCTGTTGTT AAAAAGGAGG 
     1681 AAAAAGTGCT TCATGAATTA TTTGGGATGG ACATAAGGTG CTGTGCCAGA AATGAACTGG 
     1741 GCAGGGAATG CACCAGGCTG TTCACAATAG ATCTAAATCA AACTCCTCAG ACCACATTGC 
     1801 CACAATTATT TCTTAAAGTA GGGGAACCCT TATGGATAAG GTGCAAAGCT GTTCATGTGA 
     1861 ACCATGGATT CGGGCTCACC TGGGAATTAG AAAACAAAGC ACTCGAGGAG GGCAACTACT 
     1921 TTGAGATGAG TACCTATTCA ACAAACAGAA CTATGATACG GATTCTGTTT GCTTTTGTAT 
     1981 CATCAGTGGC AAGAAACGAC ACCGGATACT ACACTTGTTC CTCTTCAAAG CATCCCAGTC 
     2041 AATCAGCTTT GGTTACCATC GTAGAAAAGG GATTTATAAA TGCTACCAAT TCAAGTGAAG 
     2101 ATTATGAAAT TGACCAATAT GAAGAGTTTT GTTTTTCTGT CAGGTTTAAA GCCTACCCAC 
     2161 AAATCAGATG TACGTGGACC TTCTCTCGAA AATCATTTCC TTGTGAGCAA AAGGGTCTTG 
     2221 ATAACGGATA CAGCATATCC AAGTTTTGCA ATCATAAGCA CCAGCCAGGA GAATATATAT 
     2281 TCCATGCAGA AAATGATGAT GCCCAATTTA CCAAAATGTT CACGCTGAAT ATAAGAAGGA 
     2341 AACCTCAAGT GCTCGCAGAA GCATCGGCAA GTCAGGCGTC CTGTTTCTCG GATGGATACC 
     2401 CATTACCATC TTGGACCTGG AAGAAGTGTT CAGACAAGTC TCCCAACTGC ACAGAAGAGA 
     2461 TCACAGAAGG AGTCTGGAAT AGAAAGGCTA ACAGAAAAGT GTTTGGACAG TGGGTGTCGA 
     2521 GCAGTACTCT AAACATGAGT GAAGCCATAA AAGGGTTCCT GGTCAAGTGC TGTGCATACA 
     2581 ATTCCCTTGG CACATCTTGT GAGACGATCC TTTTAAACTC TCCAGGCCCC TTCCCTTTCA 
     2641 TCCAAGACAA CATCTCATTC TATGCAACAA TTGGTGTTTG TCTCCTCTTC ATTGTCGTTT 
     2701 TAACCCTGCT AATTTGTCAC AAGTACAAAA AGCAATTTAG GTATGAAAGC CAGCTACAGA 
     2761 TGGTACAGGT GACCGGCTCC TCAGATAATG AGTACTTCTA CGTTGATTTC AGAGAATATG 
     2821 AATATGATCT CAAATGGGAG TTTCCAAGAG AAAATTTAGA GTTTGGGAAG GTACTAGGAT 
     2881 CAGGTGCTTT TGGAAAAGTG ATGAACGCAA CAGCTTATGG AATTAGCAAA ACAGGAGTCT 
     2941 CAATCCAGGT TGCCGTCAAA ATGCTGAAAG AAAAAGCAGA CAGCTCTGAA AGAGAGGCAC 
     3001 TCATGTCAGA ACTCAAGATG ATGACCCAGC TGGGAAGCCA CGAGAATATT GTGAACCTGC 
     3061 TGGGGGCGTG CACACTGTCA GGACCAATTT ACTTGATTTT TGAATACTGT TGCTATGGTG 
     3121 ATCTTCTCAA CTATCTAAGA AGTAAAAGAG AAAAATTTCA CAGGACTTGG ACAGAGATTT 
     3181 TCAAGGAACA CAATTTCAGT TTTTACCCCA CTTTCCAATC ACATCCAAAT TCCAGCATGC 
     3241 CTGGTTCAAG AGAAGTTCAG ATACACCCGG ACTCGGATCA AATCTCAGGG CTTCATGGGA 
     3301 ATTCATTTCA CTCTGAAGAT GAAATTGAAT ATGAAAACCA AAAAAGGCTG GAAGAAGAGG 
     3361 AGGACTTGAA TGTGCTTACA TTTGAAGATC TTCTTTGCTT TGCATATCAA GTTGCCAAAG 
     3421 GAATGGAATT TCTGGAATTT AAGTCGTGTG TTCACAGAGA CCTGGCCGCC AGGAACGTGC 
     3481 TTGTCACCCA CGGGAAAGTG GTGAAGATAT GTGACTTTGG ATTGGCTCGA GATATCATGA 
     3541 GTGATTCCAA CTATGTTGTC AGGGGCAATG CCCGTCTGCC TGTAAAATGG ATGGCCCCCG 
     3601 AAAGCCTGTT TGAAGGCATC TACACCATTA AGAGTGATGT CTGGTCATAT GGAATATTAC 
     3661 TGTGGGAAAT CTTCTCACTT GGTGTGAATC CTTACCCTGG CATTCCGGTT GATGCTAACT 
     3721 TCTACAAACT GATTCAAAAT GGATTTAAAA TGGATCAGCC ATTTTATGCT ACAGAAGAAA 
     3781 TATACATTAT AATGCAATCC TGCTGGGCTT TTGACTCAAG GAAACGGCCA TCCTTCCCTA 
     3841 ATTTGACTTC GTTTTTAGGA TGTCAGCTGG CAGATGCAGA AGAAGCGATG TATCAGAATG 
     3901 TGGATGGCCG TGTTTCGGAA TGTCCTCACA CCTACCAAAA CAGGCGACCT TTCAGCAGAG 
     3961 AGATGGATTT GGGGCTACTC TCTCCGCAGG CTCAGGTCGA AGATTCGACG CGTACGCGGC 
     4021 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4081 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4141 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4201 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4261 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4321 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4381 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4441 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4501 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4561 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4621 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4681 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4741 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4801 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4861 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4921 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4981 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5041 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5101 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5161 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5221 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5281 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5341 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5401 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5461 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5521 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5581 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5641 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5701 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5761 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5821 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5881 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5941 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6001 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6061 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6121 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6181 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6241 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6301 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6361 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6421 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6481 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6541 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6601 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6661 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6721 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6781 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6841 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6901 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6961 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7021 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7081 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7261 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7321 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7381 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7441 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7501 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7561 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7621 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7681 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7741 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7801 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//