LOCUS       RC211423                8580 bp    DNA     circular  SYN 
DEFINITION  GRIN2C (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2C (GRIN2C)
ACCESSION   NM_000835
VERSION     NM_000835.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4737..4828 
                     /label="Myc-DDK"  
     misc_feature    4858..5443 
                     /label="PolyA signal"  
     misc_feature    4915..4934 
                     /label="Restriction Enzyme"  
     misc_feature    5719..6391 
                     /label="ColE1"  
     misc_feature    6821..7615 
                     /label="Kan/Neo"  
     misc_feature    7650..7952 
                     /label="SV40 ori"  
     misc_feature    8038..8515 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4719..4725 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4719 
                     /label="GRIN2C"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGGGGCC CTGGGGCCGG CCCTGTTGCT CACCTCGCTC TTCGGTGCCT 
     1081 GGGCAGGGCT GGGTCCGGGG CAGGGCGAGC AGGGCATGAC GGTGGCCGTG GTGTTTAGCA 
     1141 GCTCAGGGCC GCCCCAGGCC CAGTTCCGTG CCCGCCTCAC CCCCCAGAGC TTCCTGGACC 
     1201 TACCCCTGGA GATCCAGCCG CTCACAGTTG GGGTCAACAC CACCAACCCC AGCAGCCTCC 
     1261 TCACCCAGAT CTGCGGCCTC CTGGGTGCTG CCCACGTCCA CGGCATTGTC TTTGAGGACA 
     1321 ACGTGGACAC CGAGGCGGTG GCCCAGATCC TTGACTTCAT CTCCTCCCAG ACCCATGTGC 
     1381 CCATCCTCAG CATCAGCGGA GGCTCTGCTG TGGTCCTCAC CCCCAAGGAG CCGGGCTCCG 
     1441 CCTTCCTGCA GCTGGGCGTG TCCCTGGAGC AGCAGCTGCA GGTGCTGTTC AAGGTGCTGG 
     1501 AAGAGTACGA CTGGAGCGCC TTCGCCGTCA TCACCAGCCT GCACCCGGGC CACGCGCTCT 
     1561 TCCTGGAGGG CGTGCGCGCC GTCGCCGACG CCAGCCACGT GAGTTGGCGG CTGCTGGACG 
     1621 TGGTCACGCT GGAGCTGGGC CCGGGAGGGC CGCGCGCGCG CACGCAGCGC CTGCTGCGCC 
     1681 AGCTCGACGC GCCCGTGTTT GTGGCCTACT GCTCGCGCGA GGAGGCCGAG GTGCTCTTCG 
     1741 CCGAGGCGGC GCAGGCCGGT CTGGTGGGGC CCGGCCACGT GTGGCTGGTG CCCAACCTGG 
     1801 CGCTGGGCAG CACCGATGCG CCCCCCGCCA CCTTCCCCGT GGGCCTCATC AGCGTCGTCA 
     1861 CCGAGAGCTG GCGCCTCAGC CTGCGCCAGA AGGTGCGCGA CGGCGTGGCC ATTCTGGCCC 
     1921 TGGGCGCCCA CAGCTACTGG CGCCAGCATG GAACCCTGCC AGCCCCGGCC GGGGACTGCC 
     1981 GTGTTCACCC TGGGCCCGTC AGCCCTGCCC GGGAGGCCTT CTACAGGCAC CTACTGAATG 
     2041 TCACCTGGGA GGGCCGAGAC TTCTCCTTCA GCCCTGGTGG GTACCTGGTC CAGCCCACCA 
     2101 TGGTGGTGAT CGCCCTCAAC CGGCACCGCC TCTGGGAGAT GGTGGGGCGC TGGGAGCATG 
     2161 GCGTCCTATA CATGAAGTAC CCCGTGTGGC CTCGCTACAG TGCCTCTCTG CAGCCTGTGG 
     2221 TGGACAGTCG GCACCTGACG GTGGCCACGC TGGAAGAGCG GCCCTTTGTC ATCGTGGAGA 
     2281 GCCCTGACCC TGGCACAGGA GGCTGTGTCC CCAACACCGT GCCCTGCCGC AGGCAGAGCA 
     2341 ACCACACCTT CAGCAGCGGG GACGTGGCCC CCTACACCAA GCTCTGCTGT AAGGGATTCT 
     2401 GCATCGACAT CCTCAAGAAG CTGGCCAGAG TGGTCAAATT CTCCTACGAC CTGTACCTGG 
     2461 TGACCAACGG CAAGCATGGC AAGCGGGTGC GCGGCGTATG GAACGGCATG ATTGGGGAGG 
     2521 TGTACTACAA GCGGGCAGAC ATGGCCATCG GCTCCCTCAC CATCAATGAG GAACGCTCCG 
     2581 AGATCGTAGA CTTCTCTGTA CCCTTTGTGG AGACGGGCAT CAGTGTGATG GTGGCTCGCA 
     2641 GCAATGGCAC CGTCTCCCCC TCGGCCTTCT TGGAGCCATA TAGCCCTGCA GTGTGGGTGA 
     2701 TGATGTTTGT CATGTGCCTC ACTGTGGTGG CCATCACCGT CTTCATGTTC GAGTACTTCA 
     2761 GCCCTGTCAG CTACAACCAG AACCTCACCA GAGGCAAGAA GTCCGGGGGC CCAGCTTTCA 
     2821 CTATCGGCAA GTCCGTGTGG CTGCTGTGGG CGCTGGTCTT CAACAACTCA GTGCCCATCG 
     2881 AGAACCCGCG GGGCACCACC AGCAAGATCA TGGTTCTGGT CTGGGCCTTC TTTGCTGTCA 
     2941 TCTTCCTCGC CAGCTACACG GCCAACCTGG CCGCCTTCAT GATCCAAGAG CAATACATCG 
     3001 ACACTGTGTC GGGCCTCAGT GACAAGAAGT TTCAGCGGCC TCAAGATCAG TACCCACCTT 
     3061 TCCGCTTCGG CACGGTGCCC AACGGCAGCA CGGAGCGGAA CATCCGCAGT AACTACCGTG 
     3121 ACATGCACAC CCACATGGTC AAGTTCAACC AGCGCTCGGT GGAGGACGCG CTCACCAGCC 
     3181 TCAAGATGGG GAAGCTGGAT GCCTTCATCT ATGATGCTGC TGTCCTCAAC TACATGGCAG 
     3241 GCAAGGACGA GGGCTGCAAG CTGGTCACCA TTGGGTCTGG CAAGGTCTTT GCTACCACTG 
     3301 GCTACGGCAT CGCCATGCAG AAGGACTCCC ACTGGAAGCG GGCCATAGAC CTGGCGCTCT 
     3361 TGCAGTTCCT GGGGGACGGA GAGACACAGA AACTGGAGAC AGTGTGGCTC TCAGGGATCT 
     3421 GCCAGAATGA GAAGAACGAG GTGATGAGCA GCAAGCTGGA CATCGACAAC ATGGCAGGCG 
     3481 TCTTCTACAT GCTGCTGGTG GCCATGGGGC TGGCCCTGCT GGTCTTCGCC TGGGAGCACC 
     3541 TGGTCTACTG GAAGCTGCGC CACTCGGTGC CCAACTCATC CCAGCTGGAC TTCCTGCTGG 
     3601 CTTTCAGCAG GGGCATCTAC AGCTGCTTCA GCGGGGTGCA GAGCCTCGCC AGCCCACCGC 
     3661 GGCAGGCCAG CCCGGACCTC ACGGCCAGCT CGGCCCAGGC CAGCGTGCTC AAGATGCTGC 
     3721 AGGCAGCCCG CGACATGGTG ACCACGGCGG GCGTAAGCAG CTCCCTGGAC CGCGCCACTC 
     3781 GCACCATCGA GAATTGGGGT GGCGGCCGCC GTGCGCCCCC ACCGTCCCCC TGCCCGACCC 
     3841 CGCGGTCTGG CCCCAGCCCA TGCCTGCCCA CCCCCGACCC GCCCCCAGAG CCGAGCCCCA 
     3901 CGGGCTGGGG ACCGCCAGAC GGGGGTCGCG CGGCGCTTGT GCGCAGGGCT CCGCAGCCCC 
     3961 CGGGCCGCCC CCCGACGCCG GGGCCGCCCC TGTCCGACGT CTCCCGAGTG TCGCGCCGCC 
     4021 CAGCCTGGGA GGCGCGGTGG CCGGTGCGGA CCGGGCACTG CGGGAGGCAC CTCTCGGCCT 
     4081 CCGAGCGGCC CCTGTCGCCC GCGCGCTGTC ACTACAGCTC CTTTCCTCGA GCCGACCGAT 
     4141 CCGGCCGCCC CTTCCTCCCG CTCTTCCCGG AGCTGGAGGA CCTGCCGCTG CTCGGTCCGG 
     4201 AGCAGCTGGC CCGGCGGGAG GCCCTGCTGC ACGCGGCCTG GGCCCGGGGC TCGCGCCCGC 
     4261 GTCACGCTTC CCTGCCCAGC TCCGTGGCCG AGGCCTTCGC TCGGCCCAGC TCGCTGCCCG 
     4321 CTGGGTGCAC CGGCCCCGCC TGCGCCCGCC CCGACGGCCA CTCGGCCTGC AGGCGCTTGG 
     4381 CGCAGGCGCA GTCAATGTGC TTGCCGATCT ACCGGGAGGC CTGCCAGGAG GGCGAGCAGG 
     4441 CAGGGGCCCC CGCCTGGCAG CACAGACAGC ACGTCTGCCT GCACGCCCAC GCCCACCTGC 
     4501 CATTTTGCTG GGGGGCTGTC TGTCCTCACC TTCCACCCTG TGCCAGCCAC GGCTCCTGGC 
     4561 TCTCCGGGGC CTGGGGGCCT CTGGGGCACA GGGGCAGGAC TCTGGGGCTG GGCACAGGCT 
     4621 ACAGAGACAG TGGGGGACTG GACGAGATCA GCAGGGTAGC CCGTGGGACG CAAGGCTTCC 
     4681 CGGGACCCTG CACCTGGAGA CGGATCTCCA GTCTGGAGTC AGAAGTGACG CGTACGCGGC 
     4741 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4801 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4861 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4921 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4981 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5041 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5101 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5161 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5221 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5281 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5341 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5401 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5461 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5521 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5581 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5641 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5701 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5761 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5821 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5881 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5941 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6001 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6061 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6121 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6181 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6241 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6301 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6361 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6421 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6481 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6541 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6601 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6661 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6721 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6781 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6841 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6901 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6961 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7021 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7081 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7141 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7201 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7261 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7321 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7381 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7441 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7501 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7561 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7621 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7681 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7741 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7801 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7861 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7981 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8041 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8101 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8161 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8221 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8281 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8341 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8401 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8461 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8521 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//