LOCUS       RC211310                8070 bp    DNA     circular  SYN 
DEFINITION  VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
ACCESSION   NM_020442
VERSION     NM_020442.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4227..4318 
                     /label="Myc-DDK"  
     misc_feature    4348..4933 
                     /label="PolyA signal"  
     misc_feature    4405..4424 
                     /label="Restriction Enzyme"  
     misc_feature    5209..5881 
                     /label="ColE1"  
     misc_feature    6311..7105 
                     /label="Kan/Neo"  
     misc_feature    7140..7442 
                     /label="SV40 ori"  
     misc_feature    7528..8005 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4209..4215 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4209 
                     /label="VARS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCATTTG CCTCTCGCCT CTTTTCGACC ACCATTTTGG GGGCTGAGGC 
     1081 ACTCACGGGG CCTCCCCAGG TTTCACTCCG TTTCTACACA GTCGGAGCCC CATGGATCTC 
     1141 CCATCTCCCG GAGGAACCGT GAAGCCAAAC AGAAGCGCCT GCGAGAGAAG CAGGCGACTC 
     1201 TGGAGGCTGA GATAGCAGGG GAGAGCAAGT CACCTGCAGA ATCCATTAAG GCCTGGAGGC 
     1261 CTAAGGAGTT AGTATTGTAT GAAATCCCTA CGAAACCCGG TGAAAAGAAA GATGTCTCTG 
     1321 GGCCCCTGCC TCCTGCATAC AGCCCCCGAT ATGTTGAGGC TGCCTGGTAC CCGTGGTGGG 
     1381 TACGAGAGGG CTTCTTCAAA CCAGAATATC AGGCCCGGCT GCCCCAAGCT ACAGGGGAGA 
     1441 CCTTTTCCAT GTGTATCCCA CCTCCCAATG TCACTGGCTC CCTGCACATT GGCCACGCAC 
     1501 TCACGGTGGC CATACAGGAT GCCCTCGTGC GCTGGCACCG GATGCGTGGG GATCAAGTGC 
     1561 TGTGGGTCCC TGGTTCAGAT CATGCAGGAA TTGCTACACA AGCTGTGGTG GAGAAACAAC 
     1621 TGTGGAAGGA ACGGGGAGTG AGGAGACATG AGCTGAGCCG GGAGGCCTTC CTTAGGGAGG 
     1681 TGTGGCAGTG GAAGGAGGCG AAAGGTGGAG AGATCTGTGA GCAGCTGCGA GCTCTGGGTG 
     1741 CCTCCCTGGA CTGGGATCGA GAGTGTTTTA CCATGGATGT TGGCTCCTCA GTGGCTGTGA 
     1801 CTGAAGCTTT TGTGCGGCTC TACAAGGCGG GGTTGCTGTA CCGGAACCAT CAGCTTGTCA 
     1861 ACTGGTCATG TGCTTTAAGA TCAGCCATCT CGGACATTGA GGTGGAGAAC CGGCCCCTGC 
     1921 CTGGCCACAC ACAGCTTCGA CTGCCTGGCT GCCCCACCCC CGTGTCTTTT GGCCTCCTAT 
     1981 TTTCTGTTGC CTTCCCCGTG GATGGAGAGC CTGATGCAGA GGTTGTGGTA GGAACCACAA 
     2041 GGCCAGAGAC GCTGCCTGGA GATGTGGCTG TGGCCGTTCA TCCAGACGAC TCGCGATACA 
     2101 CACATCTACA CGGGCGACAG CTTCGTCACC CCTTGATGGG GCAGCCTCTT CCCCTCATCA 
     2161 CAGACTATGC TGTTCAGCCA CATGTGGGCA CGGGGGCAGT GAAGGTGACT CCAGCTCACA 
     2221 GTCCTGCCGA TGCTGAGATG GGGGCCCGAC ATGGCTTGAG CCCCTTGAAT GTCATTGCGG 
     2281 AGGATGGGAC CATGACCTCT CTCTGCGGGG ACTGGCTGCA GGGTCTTCAC CGGTTTGTGG 
     2341 CCCGGGAAAA GATAATGTCT GTGCTGAGTG AACGGGGCCT GTTCCGGGGC CTCCAGAACC 
     2401 ACCCCATGGT ACTGCCCATC TGCAGCCGTT CTGGGGATGT GATAGAATAC CTGCTGAAGA 
     2461 ACCAGTGGTT TGTCCGCTGC CAGGAAATGG GGGCCCGAGC TGCCAAGGCT GTGGAGTCGG 
     2521 GGGCCCTGGA GCTCAGTCCC TCCTTCCACC AGAAGAACTG GCAGCACTGG TTTTCCCATA 
     2581 TTGGGGACTG GTGTGTCTCC CGGCAGCTGT GGTGGGGCCA TCAGATTCCA GCCTACCTGG 
     2641 TTGTAGAGGA CCATGCGCAG GGAGAAGAGG ACTGTTGGGT GGTCGGGCGG TCAGAGGCTG 
     2701 AGGCCAGAGA GGTAGCAGCG GAACTGACAG GGAGGCCAGG GGCAGAGCTG ACCCTGGAGA 
     2761 GGGATCCTGA TGTCCTAGAC ACATGGTTTT CTTCTGCCCT GTTCCCCTTT TCTGCCCTGG 
     2821 GCTGGCCCCA AGAGACCCCA GACCTTGCTC GTTTCTACCC CCTGTCACTT TTGGAAACGG 
     2881 GCAGCGACCT TCTGCTGTTC TGGGTGGGCC GCATGGTCAT GTTGGGGACC CAGCTCACAG 
     2941 GGCAGCTGCC CTTCAGCAAG GTGCTTCTTC ATCCCATGGT TCGGGACAGG CAGGGCCGGA 
     3001 AGATGAGCAA GTCCCTGGGG AATGTGCTGG ACCCAAGAGA CATCATCAGT GGGGTGGAGA 
     3061 TGCAGGTGCT GCAGGAAAAG CTGAGAAGCG GAAATTTGGA CCCTGCAGAG CTGGCCATTG 
     3121 TGGCTGCAGC ACAGAAAAAG GACTTTCCTC ACGGGATCCC TGAGTGTGGG ACAGATGCCC 
     3181 TGAGATTCAC ACTCTGCTCC CATGGAGTTC AGGCGGGCGA CTTGCACCTG TCAGTCTCTG 
     3241 AGGTCCAGAG CTGCCGACAT TTCTGCAACA AGATCTGGAA TGCTCTTCGC TTTATCCTCA 
     3301 ATGCTTTAGG GGAGAAATTT GTGCCACAGC CTGCTGAGGA GCTGTCTCCC TCCTCCCCGA 
     3361 TGGATGCCTG GATCCTGAGC CGCCTTGCCC TGGCTGCCCA GGAGTGTGAG CGGGGCTTCC 
     3421 TCACCCGAGA GCTCTCGCTC GTCACTCATG CCCTGCACCA CTTCTGGCTT CACAACCTCT 
     3481 GTGACGTCTA CCTGGAGGCT GTGAAGCCCG TGCTGTGGCA CTCGCCCCGC CCCCTGGGGC 
     3541 CCCCTCAGGT CCTGTTCTCC TGCGCTGACC TCGGCCTCCG CCTCCTGGCC CCACTGATGC 
     3601 CCTTCCTGGC TGAAGAGCTC TGGCAGAGGC TGCCCCCCAG GCCTGGTTGC CCCCCTGCCC 
     3661 CCAGCATCTC GGTTGCCCCC TACCCCAGCG CCTGCAGCTT GGAGCACTGG CGCCAGCCAG 
     3721 AGCTGGAGCG GCGCTTCTCC CGGGTCCAAG AGGTCGTGCA GGTGCTAAGG GCTCTCCAAG 
     3781 CCACGTACCA GCTCACCAAA GCCCGGCCCC GAGTGCTGCT GCAGAGCTCA GAGCCTGGGG 
     3841 ACCAGGGCCT CTTCGAGGCC TTCTTGGAGC CCCTGGGCAC CCTGGGCTAC TGTGGGGCTG 
     3901 TGGGCCTGTT ACCCCCAGGC GCAGCAGCTC CCTCCGGCTG GGCCCAGGCT CCACTCAGTG 
     3961 ACACGGCTCA AGTCTACATG GAGCTGCAGG GCCTGGTGGA CCCGCAGATC CAGCTACCTC 
     4021 TGTTAGCCGC CCGAAGGTAC AAGTTGCAGA AGCAGCTTGA TAGCCTCACA GCCAGGACCC 
     4081 CATCAGAAGG GGAGGCAGGG ACTCAGAGGC AACAAAAGCT TTCTTCCCTC CAGCTGGAAT 
     4141 TGTCAAAACT GGACAAGGCA GCCTCTCACC TCCGGCAGCT GATGGATGAG CCTCCAGCCC 
     4201 CAGGGAGCCC GGAGCTCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4261 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4321 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4381 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4441 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4501 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4561 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4621 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4681 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4741 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4801 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4861 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4921 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4981 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5041 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5101 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5161 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5221 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5281 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5341 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5401 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5461 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5521 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5581 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5641 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5701 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5761 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5821 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5881 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5941 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6001 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6061 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6121 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6181 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6241 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6301 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6361 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6421 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6481 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6541 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6601 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6661 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6721 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6781 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6841 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6901 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6961 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7021 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7081 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7141 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7201 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7261 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7321 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7441 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7501 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7561 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7621 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7681 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7741 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7801 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7861 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7921 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7981 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8041 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//