LOCUS RC211218L4 9212 bp DNA circular SYN DEFINITION TH (Myc-DDK-tagged)-Human tyrosine hydroxylase (TH), transcript variant 2 ACCESSION NM_000360 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7501..7681 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 8409..9055 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 6376..6396 /label="LR50" misc_feature 4839..5567 /label="mGFP" misc_feature 5574..5639 /label="P2A" misc_feature 7729..8348 /label="pBR322 Ori" misc_feature 5640..6239 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 4811..4818 /label="Restriction Enzyme" misc_feature 3320..4811 /label="TH" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCCACCCCC GACGCCACCA CGCCACAGGC 3361 CAAGGGCTTC CGCAGGGCCG TGTCTGAGCT GGACGCCAAG CAGGCAGAGG CCATCATGTC 3421 CCCGCGGTTC ATTGGGCGCA GGCAGAGCCT CATCGAGGAC GCCCGCAAGG AGCGGGAGGC 3481 GGCGGTGGCA GCAGTGGCCG CTGCAGTCCC CTCGGAGCCC GGGGACCCCC TGGAGGCTGT 3541 GGCCTTTGAG GAGAAGGAGG GGAAGGCCAT GCTAAACCTG CTCTTCTCCC CGAGGGCCAC 3601 CAAGCCCTCG GCGCTGTCCC GAGCTGTGAA GGTGTTTGAG ACGTTTGAAG CCAAAATCCA 3661 CCATCTAGAG ACCCGGCCCG CCCAGAGGCC GCGAGCTGGG GGCCCCCACC TGGAGTACTT 3721 CGTGCGCCTC GAGGTGCGCC GAGGGGACCT GGCCGCCCTG CTCAGTGGTG TGCGCCAGGT 3781 GTCAGAGGAC GTGCGCAGCC CCGCGGGGCC CAAGGTCCCC TGGTTCCCAA GAAAAGTGTC 3841 AGAGCTGGAC AAGTGTCATC ACCTGGTCAC CAAGTTCGAC CCTGACCTGG ACTTGGACCA 3901 CCCGGGCTTC TCGGACCAGG TGTACCGCCA GCGCAGGAAG CTGATTGCTG AGATCGCCTT 3961 CCAGTACAGG CACGGCGACC CGATTCCCCG TGTGGAGTAC ACCGCCGAGG AGATTGCCAC 4021 CTGGAAGGAG GTCTACACCA CGCTGAAGGG CCTCTACGCC ACGCACGCCT GCGGGGAGCA 4081 CCTGGAGGCC TTTGCTTTGC TGGAGCGCTT CAGCGGCTAC CGGGAAGACA ATATCCCCCA 4141 GCTGGAGGAC GTCTCCCGCT TCCTGAAGGA GCGCACGGGC TTCCAGCTGC GGCCTGTGGC 4201 CGGCCTGCTG TCCGCCCGGG ACTTCCTGGC CAGCCTGGCC TTCCGCGTGT TCCAGTGCAC 4261 CCAGTATATC CGCCACGCGT CCTCGCCCAT GCACTCCCCT GAGCCGGACT GCTGCCACGA 4321 GCTGCTGGGG CACGTGCCCA TGCTGGCCGA CCGCACCTTC GCGCAGTTCT CGCAGGACAT 4381 TGGCCTGGCG TCCCTGGGGG CCTCGGATGA GGAAATTGAG AAGCTGTCCA CGCTGTACTG 4441 GTTCACGGTG GAGTTCGGGC TGTGTAAGCA GAACGGGGAG GTGAAGGCCT ATGGTGCCGG 4501 GCTGCTGTCC TCCTACGGGG AGCTCCTGCA CTGCCTGTCT GAGGAGCCTG AGATTCGGGC 4561 CTTCGACCCT GAGGCTGCGG CCGTGCAGCC CTACCAAGAC CAGACGTACC AGTCAGTCTA 4621 CTTCGTGTCT GAGAGCTTCA GTGACGCCAA GGACAAGCTC AGGAGCTATG CCTCACGCAT 4681 CCAGCGCCCC TTCTCCGTGA AGTTCGACCC GTACACGCTG GCCATCGACG TGCTGGACAG 4741 CCCCCAGGCC GTGCGGCGCT CCCTGGAGGG TGTCCAGGAT GAGCTGGACA CCCTTGCCCA 4801 TGCGCTGAGT GCCATTGGCA GCGGACCGAC GCGTACGCGG CCGCTCGAGA TGAGCGGGGG 4861 CGAGGAGCTG TTCGCCGGCA TCGTGCCCGT GCTGATCGAG CTGGACGGCG ACGTGCACGG 4921 CCACAAGTTC AGCGTGCGCG GCGAGGGCGA GGGCGACGCC GACTACGGCA AGCTGGAGAT 4981 CAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTGG TGACCACCCT 5041 CTGCTACGGC ATCCAGTGCT TCGCCCGCTA CCCCGAGCAC ATGAAGATGA ACGACTTCTT 5101 CAAGAGCGCC ATGCCCGAGG GCTACATCCA GGAGCGCACC ATCCAGTTCC AGGACGACGG 5161 CAAGTACAAG ACCCGCGGCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 5221 GCTGAAGGGC AAGGACTTCA AGGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAG 5281 CTTCAACAGC CACAACGTGT ACATCCGCCC CGACAAGGCC AACAACGGCC TGGAGGCTAA 5341 CTTCAAGACC CGCCACAACA TCGAGGGCGG CGGCGTGCAG CTGGCCGACC ACTACCAGAC 5401 CAACGTGCCC CTGGGCGACG GCCCCGTGCT GATCCCCATC AACCACTACC TGAGCACTCA 5461 GACCAAGATC AGCAAGGACC GCAACGAGGC CCGCGACCAC ATGGTGCTCC TGGAGTCCTT 5521 CAGCGCCTGC TGCCACACCC ACGGCATGGA CGAGCTGTAC AGGTCCGGAC TCAGAGTTTG 5581 GGTAGGAAGC GGAGCTACTA ACTTCAGCCT GCTGAAGCAG GCTGGAGACG TGGAGGAGAA 5641 CCCTGGACCT ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 5701 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 5761 CGATCCGGAT CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 5821 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 5881 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 5941 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 6001 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 6061 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 6121 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 6181 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 6241 CGGTGCCTGA TGTACACCCA AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG 6301 GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT 6361 TCCACTAGGT ACAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 6421 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 6481 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 6541 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 6601 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 6661 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 6721 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 6781 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC 6841 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 6901 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 6961 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA 7021 CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA 7081 GCACAAGAGG AGGAGGAGGT GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG 7141 ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG 7201 CTAATTCACT CCCAACGAAG ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC 7261 TACTTCCCTG ATTGGCAGAA CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT 7321 GGATGGTGCT ACAAGCTAGT ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA 7381 GAGAACACCC GCTTGTTACA CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA 7441 GTATTAGAGT GGAGGTTTGA CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT 7501 CCGGACTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA 7561 CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT 7621 GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG 7681 AAAATCTCTA GCAGCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 7741 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 7801 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 7861 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 7921 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 7981 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 8041 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 8101 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 8161 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG 8221 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 8281 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 8341 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 8401 TAAGGGATTT TGGTCATGAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT 8461 CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA 8521 GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA 8581 AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG 8641 CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT 8701 AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC 8761 TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC 8821 TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA 8881 TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG 8941 TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG 9001 ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 9061 CAGTGATTTT TTTCTCCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 9121 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 9181 CGCGCACATT TCCCCGAAAA GTGCCACCTG AC //