LOCUS RC211218L1 7875 bp DNA circular SYN DEFINITION TH (Myc-DDK-tagged)-Human tyrosine hydroxylase (TH), transcript variant 2 ACCESSION NM_000360 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 6164..6344 /label="3'LTR" misc_feature 833..1013 /label="5'LTR" misc_feature 7072..7731 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV promoter" misc_feature 6503..7071 /label="ColE1" misc_feature 5039..5059 /label="LR50 primer" misc_feature 4836..4907 /label="Myc-DDK" misc_feature 3252..3271 /label="T7 promoter" misc_feature 3221..3237 /label="V2 primer" misc_feature 5048..5639 /label="WPRE" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 4811..4818 /label="Restriction Enzyme" misc_feature 3320..4811 /label="TH" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCCACCCCC GACGCCACCA CGCCACAGGC 3361 CAAGGGCTTC CGCAGGGCCG TGTCTGAGCT GGACGCCAAG CAGGCAGAGG CCATCATGTC 3421 CCCGCGGTTC ATTGGGCGCA GGCAGAGCCT CATCGAGGAC GCCCGCAAGG AGCGGGAGGC 3481 GGCGGTGGCA GCAGTGGCCG CTGCAGTCCC CTCGGAGCCC GGGGACCCCC TGGAGGCTGT 3541 GGCCTTTGAG GAGAAGGAGG GGAAGGCCAT GCTAAACCTG CTCTTCTCCC CGAGGGCCAC 3601 CAAGCCCTCG GCGCTGTCCC GAGCTGTGAA GGTGTTTGAG ACGTTTGAAG CCAAAATCCA 3661 CCATCTAGAG ACCCGGCCCG CCCAGAGGCC GCGAGCTGGG GGCCCCCACC TGGAGTACTT 3721 CGTGCGCCTC GAGGTGCGCC GAGGGGACCT GGCCGCCCTG CTCAGTGGTG TGCGCCAGGT 3781 GTCAGAGGAC GTGCGCAGCC CCGCGGGGCC CAAGGTCCCC TGGTTCCCAA GAAAAGTGTC 3841 AGAGCTGGAC AAGTGTCATC ACCTGGTCAC CAAGTTCGAC CCTGACCTGG ACTTGGACCA 3901 CCCGGGCTTC TCGGACCAGG TGTACCGCCA GCGCAGGAAG CTGATTGCTG AGATCGCCTT 3961 CCAGTACAGG CACGGCGACC CGATTCCCCG TGTGGAGTAC ACCGCCGAGG AGATTGCCAC 4021 CTGGAAGGAG GTCTACACCA CGCTGAAGGG CCTCTACGCC ACGCACGCCT GCGGGGAGCA 4081 CCTGGAGGCC TTTGCTTTGC TGGAGCGCTT CAGCGGCTAC CGGGAAGACA ATATCCCCCA 4141 GCTGGAGGAC GTCTCCCGCT TCCTGAAGGA GCGCACGGGC TTCCAGCTGC GGCCTGTGGC 4201 CGGCCTGCTG TCCGCCCGGG ACTTCCTGGC CAGCCTGGCC TTCCGCGTGT TCCAGTGCAC 4261 CCAGTATATC CGCCACGCGT CCTCGCCCAT GCACTCCCCT GAGCCGGACT GCTGCCACGA 4321 GCTGCTGGGG CACGTGCCCA TGCTGGCCGA CCGCACCTTC GCGCAGTTCT CGCAGGACAT 4381 TGGCCTGGCG TCCCTGGGGG CCTCGGATGA GGAAATTGAG AAGCTGTCCA CGCTGTACTG 4441 GTTCACGGTG GAGTTCGGGC TGTGTAAGCA GAACGGGGAG GTGAAGGCCT ATGGTGCCGG 4501 GCTGCTGTCC TCCTACGGGG AGCTCCTGCA CTGCCTGTCT GAGGAGCCTG AGATTCGGGC 4561 CTTCGACCCT GAGGCTGCGG CCGTGCAGCC CTACCAAGAC CAGACGTACC AGTCAGTCTA 4621 CTTCGTGTCT GAGAGCTTCA GTGACGCCAA GGACAAGCTC AGGAGCTATG CCTCACGCAT 4681 CCAGCGCCCC TTCTCCGTGA AGTTCGACCC GTACACGCTG GCCATCGACG TGCTGGACAG 4741 CCCCCAGGCC GTGCGGCGCT CCCTGGAGGG TGTCCAGGAT GAGCTGGACA CCCTTGCCCA 4801 TGCGCTGAGT GCCATTGGCA GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT 4861 CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT 4921 TTAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG AGGGACCTAA TAACTTCGTA 4981 TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC GGTTCCACTA GGTACAATTC 5041 GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 5101 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 5161 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 5221 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 5281 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 5341 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 5401 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG 5461 TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 5521 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 5581 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 5641 TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA AAACATGGAG CAATCACAAG 5701 TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA 5761 GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT 5821 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG 5881 AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTGGCA 5941 GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT 6001 AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA GGAGAGAACA CCCGCTTGTT 6061 ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTATTAG AGTGGAGGTT 6121 TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG CATCCGGACT GTACTGGGTC 6181 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT 6241 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA 6301 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGCAT 6361 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 6421 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 6481 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 6541 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 6601 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 6661 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 6721 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 6781 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 6841 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 6901 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 6961 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 7021 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 7081 GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG 7141 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG 7201 TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG 7261 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA 7321 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC 7381 GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC 7441 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC 7501 TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG 7561 TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA 7621 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA 7681 TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC 7741 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TCCCGCGCAC ATTTCCCCGA 7861 AAAGTGCCAC CTGAC //