LOCUS       RC211126L1                8997 bp    DNA     circular  SYN 
DEFINITION  TUT1 (Myc-DDK-tagged)-Human terminal uridylyl transferase 1, U6 snRNA-specific (TUT1)
ACCESSION   NM_022830
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7288..7468 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8196..8855 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7627..8195 
                     /label="ColE1"  
     misc_feature    6163..6183 
                     /label="LR50 primer"  
     misc_feature    5960..6031 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6172..6763 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5942..5948 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5942 
                     /label="TUT1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCGGTG GATTCGGATG TCGAATCGCT 
     3361 GCCGCGTGGG GGGTTCCGCT GCTGCCTCTG CCACGTTACT ACAGCCAACC GACCCAGCCT 
     3421 TGATGCCCAC TTGGGAGGCA GAAAGCACCG GCACCTGGTA GAACTACGAG CTGCGAGAAA 
     3481 GGCCCAGGGA CTTCGAAGTG TGTTTGTCAG TGGCTTTCCC AGGGATGTGG ATTCTGCTCA 
     3541 GCTCTCTGAG TACTTCCTAG CATTTGGACC TGTGGCCAGT GTTGTCATGG ACAAGGACAA 
     3601 GGGAGTGTTT GCCATTGTGG AGATGGGGGA CGTGGGTGCT CGAGAGGCTG TCTTGTCACA 
     3661 GTCCCAGCAC AGCCTGGGAG GACATCGCCT GCGTGTCCGC CCACGGGAGC AGAAGGAGTT 
     3721 CCAGAGCCCG GCCTCCAAAT CCCCCAAAGG AGCGGCCCCC GACAGTCACC AGCTGGCCAA 
     3781 AGCGCTAGCT GAGGCTGCAG ACGTGGGGGC ACAAATGATA AAGCTTGTGG GGCTGAGGGA 
     3841 GTTGTCCGAG GCCGAGCGGC AGCTTCGCAG CCTAGTGGTG GCCCTGATGC AGGAGGTCTT 
     3901 CACAGAGTTC TTCCCTGGCT GTGTGGTCCA CCCTTTTGGC TCTTCCATAA ATAGCTTCGA 
     3961 TGTCCATGGC TGTGATCTTG ACCTCTTCTT GGATCTGGGT GACTTGGAAG AGCCCCAGCC 
     4021 AGTCCCAAAG GCTCCAGAAT CTCCATCGCT GGACTCGGCC CTGGCTTCCC CACTGGACCC 
     4081 TCAAGCCCTG GCCTGCACCC CAGCTTCCCC TCCAGATTCA CAACCTCCTG CTTCTCCCCA 
     4141 GGATTCTGAA GCCCTGGACT TTGAAACCCC TTCCTCCTCC CTGGCGCCCC AAACTCCGGA 
     4201 CTCTGCCTTG GCCTCCGAGA CCCTTGCTTC TCCCCAGTCT CTGCCTCCAG CTTCACCACT 
     4261 GCTAGAGGAC AGGGAAGAGG GGGACCTGGG GAAGGCCTCG GAACTAGCAG AGACCCCAAA 
     4321 GGAGGAGAAA GCAGAGGGGG CAGCAATGCT GGAGCTGGTG GGATCCATTC TCCGGGGCTG 
     4381 TGTCCCTGGA GTGTATCGAG TCCAAACTGT GCCCTCTGCC CGGCGCCCTG TGGTCAAGTT 
     4441 CTGTCATCGG CCTTCAGGTC TCCACGGTGA TGTCTCCCTC AGTAACCGGC TGGCCCTGCA 
     4501 TAACTCCCGT TTCCTGAGTC TCTGCTCTGA GCTGGATGGT CGAGTCCGGC CCCTCGTGTA 
     4561 CACCCTCCGC TGCTGGGCTC AGGGTCGGGG GCTGTCAGGG AGTGGCCCCC TTCTCAGTAA 
     4621 CTACGCCCTG ACCTTGCTGG TGATCTATTT TCTTCAGACC AGGGACCCTC CTGTGTTGCC 
     4681 CACTGTGTCC CAGCTCACCC AGAAAGCAGG AGAGGGGGAA CAGGTGGAAG TCGATGGCTG 
     4741 GGACTGCAGT TTCCCCAGGG ATGCCTCAAG ACTGGAGCCC AGCATAAATG TGGAGCCCCT 
     4801 CAGTTCCCTG CTAGCCCAGT TCTTCTCCTG TGTATCTTGT TGGGATCTTC GTGGCTCCCT 
     4861 GCTGTCCCTG CGGGAGGGTC AGGCACTGCC TGTGGCAGGG GGCCTGCCTT CTAATCTCTG 
     4921 GGAGGGTCTG CGCCTTGGCC CCCTGAATCT CCAGGACCCT TTTGACCTGA GTCACAATGT 
     4981 CGCAGCCAAT GTGACCAGCC GGGTGGCTGG GCGCCTACAG AACTGCTGCC GAGCAGCAGC 
     5041 CAATTACTGC CGAAGCCTCC AGTACCAGCG CCGTTCCTCC CGGGGTCGGG ACTGGGGGCT 
     5101 GCTCCCTCTT CTGCAGCCCA GCTCCCCCAG CTCCCTGCTC TCTGCTACGC CGATCCCTTT 
     5161 ACCCCTTGCA CCCTTCACCC AGCTCACTGC TGCCCTGGTG CAGGTATTCA GGGAAGCACT 
     5221 GGGGTGCCAT ATAGAACAGG CAACCAAGAG AACGCGGTCA GAAGGAGGTG GAACTGGGGA 
     5281 GTCCTCTCAG GGAGGGACAA GCAAAAGACT CAAAGTAGAT GGACAGAAAA ACTGCTGTGA 
     5341 GGAGGGGAAA GAGGAGCAGC AGGGATGTGC AGGGGACGGT GGGGAAGACA GGGTAGAAGA 
     5401 GATGGTTATA GAGGTTGGAG AGATGGTGCA GGACTGGGCC ATGCAGAGCC CTGGGCAGCC 
     5461 AGGGGACCTG CCCCTGACCA CTGGAAAGCA TGGAGCCCCT GGAGAAGAGG GGCAGCCCAG 
     5521 CCACGCAGCC CTGGCAGAGC GGGGGCCCAA GGGACATGAG GCAGCCCAAG AATGGTCTCA 
     5581 GGGTGAGGCA GGGAAGGGGG CATCCCTGCC CTCCTCAGCG AGCTGGCGCT GTGCCTTGTG 
     5641 GCACCGAGTG TGGCAAGGGC GGCGGCGAGC CCGTAGACGC TTGCAGCAGC AAACCAAGGA 
     5701 GGGAGCTGGA GGTGGCGCTG GCACAAGAGC AGGGTGGCTG GCGACTGAGG CTCAGGTCAC 
     5761 CCAGGAGCTG AAAGGACTGA GTGGTGGCGA AGAGAGGCCA GAAACTGAGC CCCTGCTGAG 
     5821 CTTTGTGGCG TCTGTCTCCC CGGCTGACCG AATGCTCACT GTGACCCCGC TCCAGGATCC 
     5881 CCAAGGCCTG TTCCCTGATC TCCATCATTT CTTACAGGTT TTCCTCCCTC AAGCAATTCG 
     5941 ACATCTCAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC 
     6001 AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG 
     6061 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6121 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6181 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6241 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6301 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6361 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     6421 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     6481 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     6541 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     6601 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     6661 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     6721 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     6781 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     6841 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     6901 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     6961 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7021 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7081 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7141 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7201 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7261 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7321 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7381 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     7441 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     7501 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     7561 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     7621 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     7681 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     7741 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     7801 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     7861 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     7921 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     7981 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8041 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8101 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8161 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8221 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8281 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8341 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     8401 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     8461 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     8521 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     8581 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     8641 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     8701 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     8761 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     8821 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     8881 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8941 TAGAAAAATA AACAAATAGG GGTCCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
//