LOCUS       RC211018                8427 bp    DNA     circular  SYN 
DEFINITION  STON1 (Myc-DDK-tagged)-Human STON1-GTF2A1L readthrough (STON1-GTF2A1L), transcript variant 1
ACCESSION   NM_172311
VERSION     NM_172311.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4584..4675 
                     /label="Myc-DDK"  
     misc_feature    4705..5290 
                     /label="PolyA signal"  
     misc_feature    4762..4781 
                     /label="Restriction Enzyme"  
     misc_feature    5566..6238 
                     /label="ColE1"  
     misc_feature    6668..7462 
                     /label="Kan/Neo"  
     misc_feature    7497..7799 
                     /label="SV40 ori"  
     misc_feature    7885..8362 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4566..4572 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4566 
                     /label="STON1-GTF2A1L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCTCCACA AATCCAGGCA AATGGGTCAC CTTTGATGAT GATCCTGCTG 
     1081 TTCAATCTTC TCAAAAGTCA AAGAATTTTC CTCTGGAGAA TCAAGGTGTC TGTAGACCAA 
     1141 ATGGACTGAA GCTGAACCTT CCTGGCCTCA GGGAATTTCC CAGTGGATCT TCCTCCACCA 
     1201 GCAGCACTCC TCTCTCCTCC CCCATTGTAG ATTTTTATTT CAGTCCAGGA CCTCCAAGTA 
     1261 ACTCTCCTCT TTCTACACCT ACCAAAGACT TCCCAGGTTT TCCTGGCATC CCCAAAGCAG 
     1321 GGACTCATGT GCTTTATCCT ATTCCAGAAT CATCTTCAGA CAGCCCACTC GCAATATCAG 
     1381 GAGGAGAATC TTCCTTACTG CCTACCAGAC CAACATGTTT ATCCCATGCC TTGTTACCCA 
     1441 GTGACCACTC ATGTACACAT CCAACTCCCA AAGTAGGTCT TCCAGATGAA GTTAATCCTC 
     1501 AACAGGCTGA AAGCCTAGGA TTCCAAAGTG ATGATCTCCC CCAGTTTCAG TATTTTCGAG 
     1561 AGGACTGTGC TTTTTCAAGT CCATTTTGGA AAGATGAAGG CAGTGATTCC CATTTCACCC 
     1621 TTGACCCACC AGGAAGCAAA AAGATGTTCT CATCAAGAAA CAAGGAGATG CCTATTGACC 
     1681 AAAAAAGCCT AAATAAGTGT TCACTCAACT ATATCTGTGA GAAGCTTGAA CATCTCCAGT 
     1741 CAGCTGAGAA CCAAGACTCA CTTAGAAGTT TGTCTATGCA CTGTCTATGT GCTGAAGAAA 
     1801 ATGCCTCTTC CTTTGTCCCC CACACACTCT TCAGGAGTCA GCCAAAATCT GGATGGTCTT 
     1861 TCATGCTGAG AATTCCTGAG AAGAAGAATA TGATGTCTTC CCGGCAATGG GGACCAATTT 
     1921 TTCTGAAAGT TTTGCCTGGA GGAATTTTGC AGATGTATTA TGAACAGGGA TTAGAAAAAC 
     1981 CATTTAAAGA GATACAGCTT GATCCATATT GTAGGCTTTC TGAACCCAAG GTTGAGAACT 
     2041 TCAGTGTAGC AGGAAAAATC CACACTGTGA AGATTGAACA TGTGTCTTAC ACAGAAAAAA 
     2101 GGAAATACCA TTCTAAGACA GAAGTAGTTC ATGAACCTGA CATAGAGCAG ATGCTGAAGT 
     2161 TGGGGTCCAC ATCGTACCAT GACTTCCTTG ACTTTCTGAC TACTGTGGAG GAGGAGCTGA 
     2221 TGAAGTTGCC AGCTGTTTCA AAACCAAAAA AGAACTACGA GGAGCAAGAA ATTTCCTTGG 
     2281 AAATTGTGGA CAACTTTTGG GGTAAAGTCA CAAAAGAAGG AAAATTTGTT GAAAGTGCTG 
     2341 TGATAACTCA AATTTATTGC CTCTGCTTTG TGAATGGGAA CCTGGAATGC TTTTTAACCT 
     2401 TGAATGACCT TGAGTTGCCG AAGCGAGATG AATCCTATTA TGAGAAGGAC TCAGAAAAAA 
     2461 AGGGGATTGA TATTCTTGAC TACCATTTTC ATAAGTGTGT GAATGTACAA GAATTTGAGC 
     2521 AATCAAGAAT CATTAAGTTT GTACCTCTGG ATGCCTGCCG GTTTGAGCTG ATGCGTTTCA 
     2581 AGACTTTGTA TAATGGGGAT AATCTTCCCT TTTCCTTGAA GTCTGTAGTG GTTGTCCAGG 
     2641 GAGCATACGT GGAACTTCAG GCTTTTGTCA ACATGGCCTC ATTGGCGCAG AGGTCATCCT 
     2701 ATGCTGGTTC CTTAAGGTCC TGTGACAATA TAAGGATACA CTTTCCTGTC CCATCGCAGT 
     2761 GGATCAAGGC CCTTTGGACC ATGAACCTCC AGAGGCAGAA GTCTCTGAAA GCTAAAATGA 
     2821 ACCGCCGAGC ATGTCTGGGG AGTTTACATG AACTTGAATC TGAACCTGTC ATTCAAGTCA 
     2881 CTGTGGGGTC AGCAAAATAT GAGAGTGCCT ACCAGGCAGT GGTATGGAAG ATAGATCGGC 
     2941 TTCCAGACAA AAATTCAAGT CTAGATCATC CCCATTGTCT GTCATACAAA TTAGAGCTTG 
     3001 GATCAGACCA AGAAATTCCC TCTGATTGGT ATCCATTTGC TACTGTTCAG TTTTCCGTGC 
     3061 CTGACACCTG TGCCTCAAGG ACAGAGGTCA GGTCTCTGGG AGTGGAGAGT GATGTCCAGC 
     3121 CACAGAAACA TGTTCAGCAG CGAGCTTGCT ACAACATCCA GCCTAAACTC TACAGATCTG 
     3181 TAATTGAAGA TGTAATTGAA GGAGTTCGGA ATCTATTTGC TGAAGAAGGT ATAGAGGAAC 
     3241 AAGTTTTAAA AGACTTGAAG CAGCTCTGGG AAACCAAGGT TTTGCAGTCT AAAGCAACAG 
     3301 AAGACTTCTT CAGAAATAGC ATCCAATCAC CTCTGTTTAC TCTTCAGTTG CCGCACAGCT 
     3361 TGCACCAAAC ATTGCAATCG TCAACAGCAT CATTAGTTAT TCCTGCTGGT AGAACTCTTC 
     3421 CAAGTTTTAC CACAGCAGAA CTGGGCACTT CAAACTCCAG TGCAAACTTT ACTTTTCCTG 
     3481 GTTATCCCAT TCATGTACCA GCAGGTGTGA CACTACAGAC TGTATCTGGT CACCTTTATA 
     3541 AAGTCAATGT ACCAATTATG GTGACAGAGA CTTCTGGAAG AGCAGGTATT CTTCAGCATC 
     3601 CAATTCAGCA AGTATTTCAA CAGCTTGGCC AGCCTTCAGT AATACAAACT AGTGTTCCAC 
     3661 AATTGAATCC ATGGTCTCTT CAAGCAACTA CTGAAAAATC ACAGAGAATT GAAACCGTGC 
     3721 TACAGCAACC CGCAATTCTA CCTTCTGGGC CAGTAGATAG GAAACACTTA GAAAATGCCA 
     3781 CCAGTGATAT ACTTGTATCT CCTGGAAATG AGCATAAAAT CGTGCCTGAA GCTTTGTTGT 
     3841 GTCATCAGGA AAGTTCTCAC TATATCAGTC TTCCAGGTGT TGTATTTTCT CCACAGGTCT 
     3901 CTCAAACAAA TTCTAATGTG GAGTCAGTGC TCAGTGGTTC AGCTAGCATG GCTCAAAATC 
     3961 TGCATGATGA GTCCCTCTCC ACAAGCCCTC ATGGGGCTCT CCACCAGCAC GTGACTGATA 
     4021 TTCAGCTTCA TATTCTTAAA AATAGGATGT ATGGATGTGA TTCTGTAAAG CAACCAAGAA 
     4081 ATATAGAGGA ACCCAGCAAC ATACCTGTAT CAGAGAAGGA TTCTAATTCT CAGGTGGATT 
     4141 TAAGCATTCG GGTTACTGAT GATGATATTG GTGAAATAAT TCAAGTAGAT GGAAGCGGTG 
     4201 ATACATCTTC CAATGAAGAA ATAGGAAGTA CAAGAGATGC AGATGAGAAT GAATTTCTAG 
     4261 GGAATATTGA CGGGGGAGAT CTGAAGGTAC CTGAAGAAGA AGCTGACAGT ATTTCAAATG 
     4321 AGGATTCAGC CACAAACAGT AGTGATAATG AAGACCCTCA AGTAAACATT GTAGAAGAGG 
     4381 ACCCTTTAAA TTCTGGAGAT GATGTTAGTG AACAGGATGT GCCAGACCTG TTTGACACGG 
     4441 ATAATGTAAT TGTCTGTCAG TATGATAAGA TTCATCGAAG CAAGAACAAA TGGAAATTCT 
     4501 ATTTGAAAGA TGGTGTTATG TGTTTTGGAG GGAGAGACTA TGTATTTGCA AAAGCCATTG 
     4561 GTGATGCAGA GTGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//