LOCUS       RC210934                7224 bp    DNA     circular  SYN 
DEFINITION  MEFV (Myc-DDK-tagged)-Human Mediterranean fever (MEFV), transcript variant 1
ACCESSION   NM_000243
VERSION     NM_000243.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3381..3472 
                     /label="Myc-DDK"  
     misc_feature    3502..4087 
                     /label="PolyA signal"  
     misc_feature    3559..3578 
                     /label="Restriction Enzyme"  
     misc_feature    4363..5035 
                     /label="ColE1"  
     misc_feature    5465..6259 
                     /label="Kan/Neo"  
     misc_feature    6294..6596 
                     /label="SV40 ori"  
     misc_feature    6682..7159 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3363..3369 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3363 
                     /label="MEFV"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAAGACC CCTAGTGACC ATCTGCTGTC CACCCTGGAG GAGCTGGTGC 
     1081 CCTATGACTT CGAGAAGTTC AAGTTCAAGC TGCAGAACAC CAGTGTGCAG AAGGAGCACT 
     1141 CCAGGATCCC CCGGAGCCAG ATCCAGAGAG CCAGGCCGGT GAAGATGGCC ACTCTGCTGG 
     1201 TCACCTACTA TGGGGAAGAG TACGCCGTGC AGCTCACCCT GCAGGTCCTG CGGGCCATCA 
     1261 ACCAGCGCCT GCTGGCCGAG GAGCTCCACA GGGCAGCCAT TCAGGAATAT TCCACACAAG 
     1321 AAAACGGCAC AGACGATTCC GCAGCGTCCA GCTCCCTGGG GGAGAACAAG CCCAGGAGCC 
     1381 TGAAGACTCC AGACCACCCC GAGGGGAACG AGGGGAACGG CCCTCGGCCG TACGGGGGCG 
     1441 GGGCTGCCAG CCTGCGGTGC AGCCAGCCCG AGGCCGGGAG GGGGCTGTCG AGGAAGCCCC 
     1501 TGAGCAAACG CAGAGAGAAG GCATCGGAGG GCCTGGACGC GCAGGGCAAG CCTCGGACCC 
     1561 GGAGCCCGGC CCTGCCGGGC GGGAGAAGCC CCGGCCCCTG CAGGGCGCTA GAGGGGGGCC 
     1621 AGGCCGAGGT CCAGCTGCGC AGAAACGCCA GCTCCGCGGG GAGGCTGCAG GGGCTGGCGG 
     1681 GGGGCGCCCC GGGGCAGAAG GAGTGCAGGC CCTTCGAAGT GTACCTGCCC TCGGGAAAGA 
     1741 TGCGACCTAG AAGCCTTGAG GTCACCATTT CTACAGGGGA GAAGGCGCCC GCAAATCCAG 
     1801 AAATTCTCCT GACTCTAGAG GAAAAGACAG CTGCGAATCT GGACTCGGCA ACAGAACCCC 
     1861 GGGCAAGGCC CACTCCGGAT GGAGGGGCAT CTGCGGACCT GAAGGAAGGC CCTGGAAATC 
     1921 CAGAACATTC GGTCACCGGA AGGCCACCAG ACACGGCTGC GAGTCCCCGT TGCCACGCCC 
     1981 AGGAAGGAGA CCCAGTTGAC GGTACCTGTG TGCGTGATTC CTGCAGCTTC CCCGAGGCAG 
     2041 TTTCTGGGCA CCCCCAGGCC TCAGGCAGCC GCTCACCTGG CTGCCCCCGG TGCCAGGACT 
     2101 CCCATGAAAG GAAGAGCCCG GGAAGCCTAA GCCCCCAGCC CCTGCCACAG TGTAAGCGCC 
     2161 ACCTGAAGCA GGTCCAGCTG CTCTTCTGTG AGGATCACGA TGAGCCCATC TGCCTCATCT 
     2221 GCAGTCTGAG TCAGGAGCAC CAAGGCCACC GGGTGCGCCC CATTGAGGAG GTCGCCCTGG 
     2281 AACACAAGAA GAAAATTCAG AAGCAGCTGG AGCATCTGAA GAAGCTGAGA AAATCAGGGG 
     2341 AGGAGCAGCG ATCCTATGGG GAGGAGAAGG CAGTGAGCTT TCTGAAACAA ACTGAAGCGC 
     2401 TGAAGCAGCG GGTGCAGAGG AAGCTGGAGC AGGTGTACTA CTTCCTGGAA CAGCAGGAGC 
     2461 ATTTCTTTGT GGCCTCACTG GAGGACGTGG GCCAGATGGT TGGGCAGATC AGGAAGGCAT 
     2521 ATGACACCCG CGTATCCCAG GACATCGCCC TGCTCGACGC GCTGATTGGG GAACTGGAGG 
     2581 CCAAGGAGTG CCAGTCAGAA TGGGAACTTC TGCAGGACAT TGGAGACATC TTGCACAGGG 
     2641 CTAAGACAGT GCCTGTCCCT GAAAAGTGGA CCACTCCTCA AGAGATAAAA CAAAAGATCC 
     2701 AACTCCTCCA CCAGAAGTCA GAGTTTGTGG AGAAGAGCAC AAAGTACTTC TCAGAAACCC 
     2761 TGCGTTCAGA AATGGAAATG TTCAATGTTC CGGAGCTGAT TGGCGCTCAG GCACATGCTG 
     2821 TTAATGTGAT TCTGGATGCA GAAACCGCTT ACCCCAACCT CATCTTCTCT GATGATCTGA 
     2881 AGAGTGTTAG ACTTGGAAAC AAGTGGGAGA GGCTGCCTGA TGGCCCGCAA AGATTTGACA 
     2941 GCTGTATCAT TGTTCTGGGC TCTCCGAGTT TCCTCTCTGG CCGCCGTTAC TGGGAGGTGG 
     3001 AGGTTGGAGA CAAGACAGCA TGGATCCTGG GAGCCTGCAA GACATCCATA AGCAGGAAAG 
     3061 GGAACATGAC TCTGTCGCCA GAGAATGGCT ACTGGGTGGT GATAATGATG AAGGAAAATG 
     3121 AGTACCAGGC GTCCAGCGTT CCCCCGACCC GCCTGCTAAT AAAGGAGCCT CCCAAGCGTG 
     3181 TGGGCATCTT CGTGGACTAC AGAGTTGGAA GCATCTCCTT TTACAATGTG ACAGCCAGAT 
     3241 CCCACATCTA TACATTCGCC AGCTGCTCTT TCTCTGGGCC CCTTCAACCT ATCTTCAGCC 
     3301 CTGGGACACG TGATGGAGGG AAGAACACAG CTCCTCTGAC TATCTGTCCA GTGGGTGGTC 
     3361 AGGGGCCTGA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3421 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3481 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3541 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3601 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3661 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3721 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3781 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3841 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3901 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3961 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4021 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4081 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4141 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4201 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4261 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5041 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5101 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5161 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5221 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5281 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5341 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5401 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5461 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5521 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5581 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5641 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5701 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5761 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5821 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5881 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5941 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6001 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6061 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6121 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6181 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6241 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6301 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6361 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6421 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6481 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6541 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6661 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6781 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6841 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6901 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6961 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7021 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7081 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7141 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7201 TAACAAAAAT TTAACGCGAA TTTT
//