LOCUS       RC210925                8463 bp    DNA     circular  SYN 
DEFINITION  PPFIA3 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (PPFIA3)
ACCESSION   NM_003660
VERSION     NM_003660.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4620..4711 
                     /label="Myc-DDK"  
     misc_feature    4741..5326 
                     /label="PolyA signal"  
     misc_feature    4798..4817 
                     /label="Restriction Enzyme"  
     misc_feature    5602..6274 
                     /label="ColE1"  
     misc_feature    6704..7498 
                     /label="Kan/Neo"  
     misc_feature    7533..7835 
                     /label="SV40 ori"  
     misc_feature    7921..8398 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4602..4608 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4602 
                     /label="PPFIA3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGCGAG GTGATGCCCA CCATCAGCGA GGATGGCCGG CGGGGCTCGG 
     1081 CGCTGGGCCC GGACGAGGCG GGCGGGGAGC TGGAGCGCCT CATGGTCACG ATGCTCACGG 
     1141 AGCGCGAGCG CCTGCTGGAG ACGCTGCGCG AGGCACAGGA CGGGTTGGCT ACAGCGCAGC 
     1201 TGCGGCTGCG CGAGCTCGGC CACGAGAAGG ACTCGCTGCA GCGCCAGCTC AGCATCGCGC 
     1261 TGCCCCAGGA GTTTGCAGCT CTGACGAAGG AGCTGAACTT ATGTCGGGAG CAGCTGCTGG 
     1321 AGAGGGAGGA AGAGATTGCA GAGCTGAAGG CGGAACGGAA CAACACGCGG CTGCTCCTGG 
     1381 AACACCTGGA GTGCCTGGTG TCCAGGCACG AGAGGTCACT GCGCATGACC GTGGTGAAGC 
     1441 GCCAGGCCCA GTCCCCGGGT GGGGTCTCCT CGGAGGTAGA AGTGCTCAAA GCTCTAAAGT 
     1501 CTCTCTTCGA GCACCACAAG GCCCTGGATG AGAAGGTCCG GGAGCGGCTG CGGATGGCGC 
     1561 TGGAGCGCGT GGCAGTGCTC GAGGAGGAGC TGGAACTGAG CAATCAGGAG ACTCTGAACC 
     1621 TTCGAGAACA GCTGTCTAGG CGGCGGTCAG GGCTGGAAGA GCCGGGCAAG GATGGGGATG 
     1681 GGCAGACTCT TGCCAATGGC CTGGGTCCTG GCGGGGATTC CAACCGGCGC ACAGCAGAGC 
     1741 TGGAGGAGGC CCTGGAGCGG CAGCGCGCCG AGGTGTGCCA GCTGCGGGAG CGCCTGGCGG 
     1801 TGCTGTGCCG TCAGATGAGC CAGCTGGAGG AGGAGTTGGG CACCGCGCAC CGTGAGCTGG 
     1861 GCAAGGCAGA GGAAGCCAAC TCCAAGCTGC AGCGCGACCT CAAGGAGGCG CTGGCGCAGC 
     1921 GGGAAGATAT GGAGGAGCGG ATTACAACAC TGGAGAAGCG CTACCTGAGC GCCCAGCGGG 
     1981 AGGCCACGTC TCTGCACGAC GCCAACGACA AACTGGAGAA CGAGTTAGCT AGCAAGGAGT 
     2041 CGTTGTATCG GCAGAGTGAA GAGAAGAGCC GTCAGCTGGC CGAGTGGTTG GACGACGCCA 
     2101 AGCAGAAGCT GCAGCAGACG CTGCAGAAAG CGGAGACCTT GCCCGAGATA GAGGCGCAGC 
     2161 TGGCGCAGCG CGTGGCGGCG CTCAACAAGG CCGAGGAACG TCATGGGAAT TTTGAGGAGC 
     2221 GGCTTCGGCA GCTGGAGGCC CAGCTGGAAG AGAAGAATCA AGAGCTGCAG CGGGCCCGGC 
     2281 AGCGGGAGAA GATGAACGAT GACCACAATA AGCGGCTGTC CGAGACGGTG GACAAGCTGC 
     2341 TGAGCGAGTC CAACGAGCGC TTACAGCTTC ACCTCAAGGA GCGCATGGGG GCGCTGGAGG 
     2401 AGAAGAACTC CCTGAGCGAG GAGATAGCCA ACATGAAGAA GCTTCAGGAT GAGTTGCTGC 
     2461 TAAACAAGGA GCAGCTCTTG GCCGAAATGG AGCGGATGCA GATGGAGATC GACCAGCTGC 
     2521 GGGGGAGGCC ACCATCCTCC TACTCCAGGT CTCTCCCTGG CAGTGCCCTG GAGCTCCGTT 
     2581 ACTCTCAGGC ACCCACTTTA CCTTCTGGTG CCCACCTGGA TCCCTATGTG GCTGGCAGTG 
     2641 GTCGGGCAGG CAAGAGGGGC CGCTGGTCAG GGGTCAAGGA GGAGCCCTCC AAGGATTGGG 
     2701 AGCGGTCTGC CCCTTCGGGC TCCATACCAC CCCCATTCCC TGGGGAACTG GACGGCTCCG 
     2761 ATGAGGAGGA GGCAGAGGGG ATGTTTGGGG CCGAGCTGCT GTCCCCCAGT GGGCAGGCTG 
     2821 ACGTGCAGAC GCTGGCCATC ATGCTTCAGG AGCAGCTGGA GGCCATCAAC AAGGAGATCA 
     2881 AGCTGATCCA AGAGGAGAAG GAGACAACAG AACAGAGGGC AGAGGAGCTG GAGAGTCGGG 
     2941 TGTCCAGCTC TGGCTTGGAC TCGTTGGGCC GCTACCGCAG CAGCTGCTCC CTGCCCCCCT 
     3001 CCCTCACCAC CTCTACCCTT GCCAGCCCCT CCCCTCCCAG CTCTGGCCAC TCAACACCCC 
     3061 GCCTGGCACC CCCTAGCCCT GCCCGTGAGG GCACCGACAA GGCTAATCAT GTCCCTAAGG 
     3121 AGGAAGCTGG AGCTCCACGA GGGGAGGGGC CGGCCATCCC AGGAGACACC CCACCACCCA 
     3181 CTCCCCGCTC TGCCCGTCTT GAGAGAATGA CCCAGGCCTT GGCACTGCAG GCGGGGTCCC 
     3241 TGGAAGATGG GGGACCCCCA CGGGGAAGTG AGGGCACCCC AGATTCTCTG CACAAAGCCC 
     3301 CCAAGAAGAA GAGCATCAAG TCATCCATAG GCCGTCTCTT TGGCAAGAAA GAGAAGGGAC 
     3361 GAATGGGACC CCCAGGCCGG GACAGCTCTT CTCTGGCTGG AACACCCTCA GATGAGACAC 
     3421 TGGCCACTGA CCCTCTGGGG CTAGCCAAGC TGACAGGCCC AGGAGACAAG GACCGAAGGA 
     3481 ACAAGAGGAA GCATGAACTC CTGGAGGAGG CCTGCCGCCA GGGCCTACCT TTTGCTGCCT 
     3541 GGGACGGGCC CACTGTGGTG TCCTGGCTGG AGCTGTGGGT GGGCATGCCT GCCTGGTATG 
     3601 TGGCCGCCTG CCGGGCCAAT GTCAAGAGCG GTGCCATCAT GGCCAACCTG TCAGACACGG 
     3661 AGATCCAGCG CGAGATCGGC ATCAGCAACC CGCTGCACCG ACTCAAGCTA CGCCTCGCCA 
     3721 TCCAGGAGAT GGTCTCGCTC ACCTCGCCCT CAGCCCCCGC CTCCTCCCGC ACTTCCACAG 
     3781 GAAACGTGTG GATGACACAC GAGGAGATGG AGTCCCTTAC GGCCACGACC AAGCCCGAGA 
     3841 CCAAGGAGAT CAGCTGGGAG CAGATCCTGG CATATGGCGA CATGAACCAC GAGTGGGTGG 
     3901 GGAACGACTG GCTGCCCAGC CTGGGGCTGC CCCAATACCG CAGCTACTTC ATGGAGTCGC 
     3961 TGGTGGACGC TCGAATGTTA GATCACCTTA ACAAGAAGGA GCTCCGGGGC CAACTCAAGA 
     4021 TGGTGGACAG CTTTCACAGG GTGAGTCTAC ATTATGGGAT TATGTGCCTG AAACGGCTCA 
     4081 ACTATGACCG GAAGGACCTG GAGCGGAGGC GGGAAGAAAG TCAGACCCAG ATCCGAGACG 
     4141 TGATGGTGTG GTCCAATGAG CGGGTCATGG GTTGGGTGTC CGGGCTGGGC CTGAAGGAAT 
     4201 TTGCCACGAA CCTCACGGAG AGCGGGGTAC ACGGGGCACT GCTCGCCCTG GACGAGACCT 
     4261 TCGACTACTC CGACCTGGCC TTGCTCCTGC AGATCCCCAC GCAGAATGCA CAGGCCCGGC 
     4321 AGCTTCTGGA GAAGGAATTC AGCAACCTTA TCTCCTTAGG CACAGACAGG CGGCTGGACG 
     4381 AGGACAGCGC CAAGTCTTTC AGCCGCTCCC CATCCTGGCG GAAGATGTTC CGGGAGAAGG 
     4441 ACCTCCGAGG CGTAACTCCC GACTCAGCTG AGATGTTGCC CCCCAACTTT CGTTCGGCTG 
     4501 CAGCGGGAGC CCTGGGCTCT CCGGGGCTCC CTCTCCGCAA GCTGCAGCCA GAAGGCCAGA 
     4561 CTTCTGGGAG TTCCCGGGCA GACGGCGTTT CGGTCCGGAC CTATTCCTGC ACGCGTACGC 
     4621 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4681 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4741 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4801 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4861 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4921 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4981 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5041 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5101 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5161 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5221 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5281 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5341 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5401 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5461 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5521 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5581 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5641 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5701 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5761 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5821 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5881 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5941 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6001 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6061 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6121 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6181 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6241 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6301 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6361 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6421 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6481 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6541 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6601 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6661 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6721 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6781 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6841 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6901 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6961 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7021 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7081 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7141 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7201 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7261 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7321 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7381 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7441 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7501 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7561 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7621 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7681 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7861 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7921 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7981 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8041 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8101 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8161 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8221 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8281 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8341 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8401 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8461 TTT
//