LOCUS       RC210840                8376 bp    DNA     circular  SYN 
DEFINITION  TRPM5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 5 (TRPM5)
ACCESSION   NM_014555
VERSION     NM_014555.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4533..4624 
                     /label="Myc-DDK"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    4711..4730 
                     /label="Restriction Enzyme"  
     misc_feature    5515..6187 
                     /label="ColE1"  
     misc_feature    6617..7411 
                     /label="Kan/Neo"  
     misc_feature    7446..7748 
                     /label="SV40 ori"  
     misc_feature    7834..8311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4515..4521 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4515 
                     /label="TRPM5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGATGTC CAAGGCCCCC GTCCCGGAAG CCCCGGGGAT GCTGAAGACC 
     1081 GGCGGGAGCT GGGCTTGCAC AGGGGCGAGG TCAACTTTGG AGGGTCTGGG AAGAAGCGAG 
     1141 GCAAGTTTGT ACGGGTGCCG AGCGGAGTGG CCCCGTCTGT GCTCTTTGAC CTGCTGCTTG 
     1201 CTGAGTGGCA CCTGCCAGCC CCCAACCTGG TGGTGTCCCT GGTGGGTGAG GAGCAGCCTT 
     1261 TCGCCATGAA GTCCTGGCTG CGGGATGTGC TGCGCAAGGG GCTGGTGAAG GCGGCTCAGA 
     1321 GCACAGGAGC CTGGATCCTG ACCAGTGCCC TCCGCGTGGG CCTGGCCAGG CATGTCGGGC 
     1381 AGGCCGTGCG CGACCACTCG CTGGCCAGCA CGTCCACCAA GGTCCGTGTG GTTGCTGTCG 
     1441 GCATGGCCTC GCTGGGCCGC GTCCTGCACC GCCGCATTCT GGAGGAGGCC CAGGAGGATT 
     1501 TTCCTGTCCA CTACCCTGAG GATGACGGCG GCAGCCAGGG CCCCCTCTGT TCACTGGACA 
     1561 GCAACCTCTC CCACTTCATC CTGGTGGAGC CAGGCCCCCC GGGGAAGGGC GATGGGCTGA 
     1621 CGGAGCTGCG GCTGAGGCTG GAGAAGCACA TCTCGGAGCA GAGGGCGGGC TACGGGGGCA 
     1681 CTGGCAGCAT CGAGATCCCT GTCCTCTGCT TGCTGGTCAA TGGTGATCCC AACACCTTGG 
     1741 AGAGGATCTC CAGGGCCGTG GAGCAGGCTG CCCCGTGGCT GATCCTGGCA GGCTCGGGGG 
     1801 GCATCGCCGA TGTGCTTGCT GCCCTAGTGA ACCAGCCCCA CCTCCTGGTG CCCAAGGTGG 
     1861 CCGAGAAGCA GTTTAAGGAG AAGTTCCCCA GCAAGCATTT CTCTTGGGAG GACATCGTGC 
     1921 GCTGGACCAA GCTGCTGCAG AACATCACCT CACACCAGCA CCTGCTCACC GTGTATGACT 
     1981 TCGAGCAGGA GGGCTCCGAG GAGCTGGACA CGGTCATCCT GAAGGCGCTG GTGAAAGCCT 
     2041 GCAAGAGCCA CAGCCAGGAG CCTCAGGACT ATCTGGATGA GCTCAAGCTG GCCGTGGCCT 
     2101 GGGACCGCGT GGACATCGCC AAGAGTGAGA TCTTCAATGG GGACGTGGAG TGGAAGTCCT 
     2161 GTGACCTGGA GGAGGTGATG GTGGACGCCC TGGTCAGCAA CAAGCCCGAG TTTGTGCGCC 
     2221 TCTTTGTGGA CAACGGCGCA GACGTGGCCG ACTTCCTGAC GTATGGGCGG CTGCAGGAGC 
     2281 TCTACCGCTC TGTGTCACGC AAGAGCCTGC TCTTCGACCT GCTGCAGCGG AAGCAGGAGG 
     2341 AGGCCCGGCT GACGCTGGCC GGCCTGGGCA CCCAGCAGGC CCGGGAGCCA CCCGCGGGGC 
     2401 CACCGGCCTT CTCCCTGCAC GAGGTCTCCC GCGTACTCAA GGACTTCCTG CAGGACGCCT 
     2461 GCCGAGGCTT CTACCAGGAC GGCCGGCCAG GGGACCGCAG GAGGGCGGAG AAGGGCCCGG 
     2521 CCAAGCGGCC CACGGGCCAG AAGTGGCTGC TGGACCTGAA CCAGAAGAGC GAGAACCCCT 
     2581 GGCGGGACCT GTTCCTGTGG GCCGTGCTGC AGAACCGCCA CGAGATGGCC ACCTACTTCT 
     2641 GGGCCATGGG CCAGGAAGGT GTGGCAGCCG CACTGGCCGC CTGCAAAATC CTCAAAGAGA 
     2701 TGTCGCACCT GGAGACGGAG GCCGAGGCGG CCCGAGCCAC GCGCGAGGCG AAATACGAGC 
     2761 GGCTGGCCCT TGACCTCTTC TCCGAGTGCT ACAGCAACAG TGAGGCCCGC GCCTTCGCCC 
     2821 TGCTGGTGCG CCGGAACCGC TGCTGGAGCA AGACCACCTG CCTGCACCTG GCCACCGAGG 
     2881 CTGACGCCAA GGCCTTCTTT GCCCACGACG GCGTTCAGGC CTTCCTGACC AGGATCTGGT 
     2941 GGGGGGACAT GGCCGCAGGC ACGCCCATCC TGCGGCTGCT AGGAGCCTTC CTCTGCCCCG 
     3001 CCCTCGTCTA TACCAACCTC ATCACCTTCA GTGAGGAAGC TCCCCTGAGG ACAGGCCTGG 
     3061 AGGACCTGCA GGACCTGGAC AGCCTGGACA CGGAGAAGAG CCCGCTGTAT GGCCTGCAGA 
     3121 GCCGGGTGGA GGAGCTGGTG GAGGCGCCGA GGGCTCAGGG TGACCGAGGC CCACGTGCTG 
     3181 TCTTCCTGCT CACACGCTGG CGGAAATTCT GGGGCGCTCC CGTGACTGTG TTCCTGGGGA 
     3241 ACGTGGTCAT GTACTTCGCC TTCCTCTTCC TGTTCACCTA CGTCCTGCTG GTGGACTTCA 
     3301 GGCCGCCCCC CCAGGGCCCC TCAGGGCCCG AGGTCACCCT CTACTTCTGG GTCTTTACGC 
     3361 TGGTGCTGGA GGAAATCCGG CAGGGCTTCT TCACAGACGA GGACACACAC CTGGTGAAGA 
     3421 AGTTCACACT GTATGTGGGG GACAACTGGA ACAAGTGTGA CATGGTGGCC ATCTTCCTGT 
     3481 TCATCGTGGG TGTCACCTGC AGGATGCTGC CGTCGGCGTT TGAGGCTGGC CGCACAGTCC 
     3541 TCGCCATGGA CTTCATGGTG TTCACGCTGC GGCTGATCCA TATCTTTGCC ATACACAAGC 
     3601 AGCTGGGCCC CAAGATCATC GTGGTAGAGC GCATGATGAA GGACGTCTTC TTCTTCCTCT 
     3661 TCTTTCTGAG CGTGTGGCTC GTGGCCTACG GTGTCACCAC CCAGGCGCTG CTGCACCCCC 
     3721 ATGACGGCCG CCTGGAGTGG ATCTTCCGCC GGGTGCTCTA CCGGCCCTAC CTGCAGATCT 
     3781 TCGGCCAGAT CCCACTGGAC GAGATTGATG AAGCCCGTGT GAACTGCTCC ACCCACCCAC 
     3841 TGCTGCTGGA GGACTCACCA TCCTGCCCCA GCCTCTATGC CAACTGGCTG GTCATCCTCC 
     3901 TGCTGGTCAC CTTCCTGTTG GTCACCAATG TGCTGCTCAT GAACCTGCTC ATCGCCATGT 
     3961 TCAGCTACAC GTTCCAGGTG GTGCAGGGCA ACGCAGACAT GTTCTGGAAG TTCCAGCGCT 
     4021 ACAACCTGAT TGTGGAGTAC CACGAGCGCC CCGCCCTGGC CCCGCCCTTC ATCCTGCTCA 
     4081 GCCACCTGAG CCTGACGCTC CGCCGGGTCT TCAAGAAGGA GGCTGAGCAC AAGCGGGAGC 
     4141 ACCTGGAGAG AGACCTGCCA GACCCCCTGG ACCAGAAGGT CGTCACCTGG GAGACAGTCC 
     4201 AGAAGGAGAA CTTCCTGAGC AAGATGGAGA AGCGGAGGAG GGACAGCGAG GGGGAGGTGC 
     4261 TGCGGAAAAC CGCCCACAGA GTGGACTTCA TTGCCAAGTA CCTCGGGGGT CTGAGAGAGC 
     4321 AAGAAAAGCG CATCAAGTGT CTGGAGTCAC AGATCAACTA CTGCTCGGTG CTCGTGTCCT 
     4381 CCGTGGCTGA CGTGCTGGCC CAGGGTGGCG GCCCCCGGAG CTCTCAGCAC TGTGGCGAGG 
     4441 GAAGCCAGCT GGTGGCTGCT GACCACAGAG GTGGTTTAGA TGGCTGGGAA CAACCCGGGG 
     4501 CTGGCCAGCC TCCCTCGGAC ACCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4561 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5341 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5461 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5521 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5581 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5641 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5761 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5821 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5881 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5941 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6181 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6241 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6301 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6361 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6421 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6481 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6541 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6601 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6661 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6721 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6781 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6841 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6901 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6961 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7021 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7081 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7141 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7201 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7261 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7321 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7381 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7441 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7501 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7561 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7621 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7801 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7861 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7921 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7981 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8041 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8101 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8161 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8221 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8281 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8341 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//