LOCUS       RC210832                8262 bp    DNA     circular  SYN 
DEFINITION  NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1
ACCESSION   NM_005337
VERSION     NM_005337.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4419..4510 
                     /label="Myc-DDK"  
     misc_feature    4540..5125 
                     /label="PolyA signal"  
     misc_feature    4597..4616 
                     /label="Restriction Enzyme"  
     misc_feature    5401..6073 
                     /label="ColE1"  
     misc_feature    6503..7297 
                     /label="Kan/Neo"  
     misc_feature    7332..7634 
                     /label="SV40 ori"  
     misc_feature    7720..8197 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4401..4407 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4401 
                     /label="NCKAP1L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTGACA TCTGCTTACC AGCATAAATT AGCAGAGAAG CTCACTATCC 
     1081 TGAATGATCG CGGTCAGGGG GTTCTCATCC GTATGTATAA CATCAAGAAG ACTTGTTCAG 
     1141 ACCCCAAATC TAAGCCACCT TTCTTACTGG AAAAGTCCAT GGAACCATCT CTCAAGTATA 
     1201 TCAACAAGAA ATTTCCCAAC ATAGATGTCC GAAACAGCAC GCAACATTTA GGACCAGTAC 
     1261 ATCGTGAAAA AGCCGAGATA ATTAGATTCC TCACCAACTA CTACCAGTCA TTTGTGGATG 
     1321 TCATGGAATT TCGGGATCAT GTATATGAAC TTCTCAACAC CATTGATGCC TGCCAGTGCC 
     1381 ATTTTGATAT CAATCTCAAC TTTGATTTCA CTCGGAGTTA CCTGGACTTG ATTGTAACTT 
     1441 ACACCTCAGT CATTTTACTT CTGTCACGGA TTGAAGATCG GCGGATACTC ATTGGCATGT 
     1501 ACAATTGTGC CCATGAGATG CTGCATGGGC ATGGTGACCC CAGTTTTGCC CGTCTGGGTC 
     1561 AGATGGTCTT GGAGTATGAC CACCCTCTGA AGAAGCTGAC AGAAGAGTTT GGGCCTCACA 
     1621 CAAAGGCTGT GAGTGGAGCC CTCCTCTCTT TGCATTTCCT CTTTGTCCGA AGAAACCAGG 
     1681 GGGCTGAGCA GTGGCGCAGT GCCCAACTTC TAAGCCTCAT CAGCAACCCC CCAGCCATGA 
     1741 TTAACCCTGC TAATTCAGAT ACAATGGCCT GTGAGTATCT GTCTGTGGAA GTAATGGAGC 
     1801 GCTGGATTAT CATTGGGTTT CTTCTTTGTC ATGGGTGCCT CAACTCCAAT AGCCAGTGCC 
     1861 AGAAGCTGTG GAAGCTGTGT CTGCAGGGCT CCCTCTACAT CACCCTTATC CGTGAGGATG 
     1921 TGCTGCAGGT GCACAAAGTC ACCGAGGACC TGTTTAGCAG TTTGAAAGGG TATGGCAAGA 
     1981 GAGTGGCAGA CATAAAGGAG AGCAAGGAAC ATGTAATTGC AAACAGTGGC CAGTTTCATT 
     2041 GTCAACGGCG GCAATTTCTG CGGATGGCAG TGAAGGAGCT GGAGACTGTG TTGGCTGATG 
     2101 AACCGGGACT ACTGGGTCCT AAGGCTCTTT TTGCTTTCAT GGCCCTGTCC TTCATTCGTG 
     2161 ATGAGGTCAC CTGGCTGGTT CGCCACACAG AGAATGTCAC CAAGACAAAG ACACCTGAGG 
     2221 ACTATGCTGA CTCGAGCATT GCAGAGCTAC TTTTCTTGTT GGAGGGGATT AGGTCTCTGG 
     2281 TCCGAAGACA CATCAAAGTG ATACAGCAAT ACCACCTTCA GTACTTGGCA AGATTTGATG 
     2341 CTCTTGTGCT CAGTGACATC ATTCAGAACT TGTCTGTGTG TCCAGAGGAG GAGTCCATCA 
     2401 TCATGTCCTC ATTCGTCAGT ATCCTCTCCT CTCTGAATCT CAAACAAGTT GATAATGGAG 
     2461 AAAAATTTGA ATTCTCAGGA TTGAGGCTGG ACTGGTTCCG CCTACAGGCA TACACTAGCG 
     2521 TGGCTAAGGC CCCTCTGCAC CTGCATGAGA ACCCTGACTT AGCCAAGGTG ATGAACCTCA 
     2581 TTGTCTTCCA CTCCCGAATG CTGGACTCCG TAGAAAAATT GCTGGTGGAA ACTTCTGATC 
     2641 TGTCTACTTT CTGCTTTCAT CTTCGTATCT TTGAGAAGAT GTTTGCCATG ACCTTGGAGG 
     2701 AATCTGCCAT GTTGCGTTAT GCCATTGCTT TCCCCCTGAT TTGTGCTCAC TTTGTCCACT 
     2761 GCACTCATGA GATGTGCCCA GAGGAGTACC CCCACCTCAA GAACCATGGT CTTCACCACT 
     2821 GCAACTCCTT CCTGGAAGAG TTGGCCAAGC AGACCAGCAA TTGCGTCCTG GAGATCTGTG 
     2881 CTGAGCAGCG AAACCTGAGC GAGCAGCTTC TACCTAAGCA CTGTGCCACT ACAATCAGCA 
     2941 AAGCCAAGAA CAAGAAAACC AGGAAGCAGA GGCAGACTCC CAGAAAAGGA GAGCCCGAGA 
     3001 GGGACAAGCC AGGAGCTGAG AGTCACCGGA AGAACCGCAG CATTGTCACC AACATGGACA 
     3061 AGCTACACCT AAACTTGACA GAACTGGCAC TGACAATGAA TCATGTATAC AGTTTCTCCG 
     3121 TGTTTGAACA TACTATCTTC CCTTCTGAGT ACCTCAGCAG CCACCTGGAG GCCAGACTCA 
     3181 ACAGAGCCAT TGTGTGGCTG GCTGGCTACA ATGCCACGAC CCAGGAGATC GTACGGCCTT 
     3241 CTGAGCTGTT GGCAGGAGTC AAAGCATACA TTGGTTTCAT ACAGTCACTG GCCCAGTTTT 
     3301 TGGGTGCAGA TGCTTCCAGA GTCATCCGCA ACGCCCTCCT GCAGCAGACA CAACCACTGG 
     3361 ATTCCTGTGG GGAACAGACA ATCACCACAC TCTACACAAA CTGGTACCTG GAAAGTCTGC 
     3421 TTAGACAGGC AAGCAGTGGG ACCATCATCC TCTCCCCAGC CATGCAGGCC TTCGTCAGCC 
     3481 TGCCCAGAGA AGGGGAGCAG AACTTCAGTG CAGAGGAGTT CTCTGACATC TCTGAGATGC 
     3541 GGGCCTTGGC AGAACTCCTG GGCCCCTATG GAATGAAGTT CCTGAGTGAA AACCTGATGT 
     3601 GGCATGTGAC CTCTCAGATT GTGGAGCTGA AGAAGCTGGT GGTGGAAAAC ATGGACATAC 
     3661 TTGTTCAGAT CAGATCCAAC TTTAGCAAGC CGGACTTGAT GGCTTCCCTG CTGCCCCAGC 
     3721 TGACAGGGGC TGAAAATGTG CTAAAGCGCA TGACCATCAT TGGGGTTATC CTCAGTTTCA 
     3781 GGGCCATGGC CCAAGAGGGA CTTCGGGAGG TTTTCTCCTC CCACTGCCCA TTTCTTATGG 
     3841 GTCCCATTGA GTGCTTGAAG GAGTTTGTCA CTCCAGACAC AGACATCAAG GTGACCTTGA 
     3901 GTATCTTTGA GCTGGCATCT GCTGCAGGTG TGGGCTGTGA CATTGACCCA GCCTTGGTGG 
     3961 CTGCCATTGC TAATCTGAAA GCTGATACTT CATCTCCTGA GGAGGAATAT AAGGTGGCCT 
     4021 GCCTGCTCTT GATCTTTCTG GCAGTTTCCC TCCCACTCCT TGCCACTGAC CCTTCTTCCT 
     4081 TTTATAGCAT TGAGAAGGAT GGTTACAACA ACAATATTCA TTGCTTGACC AAAGCCATCA 
     4141 TCCAGGTGTC TGCTGCCCTC TTCACGCTCT ACAACAAGAA CATTGAAACT CACCTCAAGG 
     4201 AATTTCTGGT GGTGGCCTCT GTCAGCCTCT TGCAGCTGGG CCAGGAGACT GACAAGCTTA 
     4261 AAACCAGAAA TCGAGAATCC ATTTCTCTGC TCATGCGCTT GGTGGTGGAG GAGTCATCCT 
     4321 TCCTGACCCT GGACATGCTG GAGTCCTGTT TCCCTTATGT CCTGCTTCGA AATGCCTATC 
     4381 GGGAGGTGTC TCGGGCCTTC CACCTAAACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4441 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4501 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4561 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4621 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4681 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4741 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4801 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4861 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4921 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4981 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5041 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5101 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5161 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5221 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5281 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5341 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5401 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5461 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5521 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5581 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5641 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5701 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5761 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5821 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5881 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5941 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6001 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6061 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6121 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6181 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6241 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6301 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6361 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6421 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6481 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6541 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6601 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6661 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6721 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6781 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6841 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6901 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6961 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7021 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7081 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7141 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7201 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7261 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7321 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7381 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7441 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7501 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7681 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7741 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7801 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7861 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7921 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7981 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8041 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8101 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8161 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8221 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//