LOCUS       RC210830                8664 bp    DNA     circular  SYN 
DEFINITION  ARHGAP29 (Myc-DDK-tagged)-Human Rho GTPase activating protein 29 (ARHGAP29)
ACCESSION   NM_004815
VERSION     NM_004815.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4821..4912 
                     /label="Myc-DDK"  
     misc_feature    4942..5527 
                     /label="PolyA signal"  
     misc_feature    4999..5018 
                     /label="Restriction Enzyme"  
     misc_feature    5803..6475 
                     /label="ColE1"  
     misc_feature    6905..7699 
                     /label="Kan/Neo"  
     misc_feature    7734..8036 
                     /label="SV40 ori"  
     misc_feature    8122..8599 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4803..4809 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4803 
                     /label="ARHGAP29"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGCTCAC AAACAGAAAA AGACAAAGAA AAAACGTGCT TGGGCATCAG 
     1081 GTCAACTCTC TACTGATATT ACAACTTCTG AAATGGGGCT CAAGTCCTTA AGTTCCAACT 
     1141 CTATTTTTGA TCCGGATTAC ATCAAGGAGT TGGTGAATGA TATCAGGAAG TTCTCCCACA 
     1201 TGTTACTATA TTTGAAAGAA GCCATATTTT CAGACTGTTT TAAAGAAGTT ATTCATATAC 
     1261 GTCTAGAGGA ACTGCTCCGT GTTTTAAAGT CTATAATGAA TAAACATCAG AACCTCAATT 
     1321 CTGTTGATCT TCAAAATGCT GCAGAAATGC TCACTGCAAA AGTGAAAGCT GTGAACTTCA 
     1381 CAGAAGTTAA TGAAGAAAAC AAAAACGATC TCTTCCAGGA AGTGTTTTCT TCTATTGAAA 
     1441 CTTTGGCATT TACCTTTGGA AATATCCTTA CAAACTTCCT TATGGGAGAT GTAGGCAATG 
     1501 ATTCATTATT GCGACTGCCT GTTTCTCGAG AAACTAAGTC GTTTGAAAAT GTTTCTGTGG 
     1561 AATCAGTGGA CTCATCCAGT GAAAAAGGAA ATTTTTCCCC TTTAGAACTA GACAACGTGC 
     1621 TGTTAAAGAA CACTGACTCT ATCGAGCTGG CTTTGTCATA TGCTAAAACT TGGTCAAAAT 
     1681 ATACTAAGAA CATAGTTTCA TGGGTTGAAA AAAAGCTTAA CTTGGAATTG GAGTCCACTA 
     1741 GAAATATGGT CAAGTTGGCA GAGGCAACTA GAACTAACAT TGGAATTCAG GAGTTCATGC 
     1801 CACTGCAGTC TCTGTTTACT AATGCTCTTC TTAATGATAT AGAAAGCAGT CACCTTTTAC 
     1861 AACAAACAAT TGCAGCTCTC CAGGCTAACA AATTTGTGCA GCCTCTACTT GGAAGGAAAA 
     1921 ATGAAATGGA AAAACAAAGG AAAGAAATAA AAGAGCTTTG GAAACAGGAG CAAAATAAAA 
     1981 TGCTTGAAGC AGAGAATGCT CTCAAAAAGG CAAAATTATT ATGCATGCAA CGTCAAGATG 
     2041 AATATGAGAA AGCAAAGTCT TCCATGTTTC GTGCAGAAGA GGAGCATCTG TCTTCAAGTG 
     2101 GCGGATTAGC AAAAAATCTC AACAAGCAAC TAGAAAAAAA GCGAAGGTTG GAAGAGGAGG 
     2161 CTCTCCAAAA AGTAGAAGAA GCAAATGAAC TTTACAAAGT TTGTGTGACA AATGTTGAAG 
     2221 AAAGAAGAAA TGATCTAGAA AATACCAAAA GAGAAATTTT AGCACAACTC CGGACACTTG 
     2281 TTTTCCAGTG TGATCTTACC CTTAAAGCTG TAACAGTTAA CCTCTTCCAC ATGCAGCATC 
     2341 TGCAGGCTGC TTCCCTTGCA GACAGTTTAC AGTCTCTCTG TGATAGTGCC AAACTCTATG 
     2401 ACCCAGGCCA AGAGTACAGT GAATTTGTCA AGGCCACAAA TTCAACTGAA GAAGAAAAAG 
     2461 TTGATGGAAA TGTAAATAAA CATTTAAATA GTTCCCAACC TTCAGGATTT GGACCTGCCA 
     2521 ACTCTTTAGA GGATGTTGTA CGCCTTCCTG ACAGTTCTAA TAAAATTGAA GAGGACAGAT 
     2581 GCTCTAACAG TGCAGATATA ACAGGTCCTT CCTTTATAAG ATCATGGACA TTTGGGATGT 
     2641 TTAGTGATTC TGAGAGCACT GGAGGGAGCA GCGAATCTAG ATCTCTGGAT TCAGAATCTA 
     2701 TAAGTCCAGG AGACTTTCAT CGAAAACTTC CACGAACACC ATCCAGTGGA ACTATGTCCT 
     2761 CTGCAGATGA TCTAGATGAA AGAGAGCCAC CTTCCCCTTC AGAAACTGGA CCCAATTCCC 
     2821 TTGGAACATT TAAGAAAACA TTGATGTCAA AGGCAGCTCT CACACACAAG TTTCGCAAAT 
     2881 TGAGATCCCC CACGAAATGT AGGGATTGTG AAGGCATTGT AGTGTTCCAA GGTGTTGAAT 
     2941 GTGAAGAGTG TCTCCTTGTT TGTCATCGAA AGTGTTTGGA AAATTTAGTC ATTATTTGTG 
     3001 GTCATCAGAA ACTTCCAGGA AAAATACACT TATTTGGAGC AGAATTCACA CAAGTTGCAA 
     3061 AAAAGGAACC AGATGGTATC CCTTTTATAC TCAAAATATG TGCCTCAGAG ATTGAAAATA 
     3121 GAGCTTTGTG TCTACAGGGA ATTTATCGTG TGTGTGGAAA CAAAATAAAA ACTGAAAAAT 
     3181 TGTGTCAAGC TTTGGAAAAT GGAATGCACT TGGTAGATAT TTCAGAATTT AGTTCACATG 
     3241 ATATCTGTGA CGTCTTGAAA TTATACCTTC GGCAGCTCCC AGAACCATTT ATTTTATTTC 
     3301 GATTGTACAA GGAATTTATA GACCTTGCAA AAGAGATCCA ACATGTAAAT GAAGAACAAG 
     3361 AGACAAAAAA GAATAGTCTT GAAGACAAAA AATGGCCAAA TATGTGTATA GAAATAAACC 
     3421 GAATTCTTCT AAAAAGCAAA GACCTTCTAA GACAATTGCC AGCATCAAAT TTTAACAGTC 
     3481 TTCATTTCCT TATAGTACAT CTAAAGCGGG TAGTAGATCA TGCAGAAGAA AACAAGATGA 
     3541 ACTCCAAAAA CTTGGGGGTG ATATTTGGAC CAAGTCTCAT TAGGCCAAGG CCCACAACTG 
     3601 CTCCTATCAC CATCTCCTCC CTTGCAGAGT ATTCAAATCA AGCACGCTTG GTAGAGTTTC 
     3661 TCATTACTTA CTCACAGAAG ATCTTCGATG GGTCCCTACA ACCACAAGAT GTTATGTGTA 
     3721 GCATAGGTGT TGTTGATCAA GGCTGTTTTC CAAAGCCTCT GTTATCACCA GAAGAAAGAG 
     3781 ACATTGAACG TTCCATGAAG TCACTATTTT TTTCTTCAAA GGAAGATATC CATACTTCAG 
     3841 AGAGTGAAAG CAAAATTTTT GAACGAGCTA CATCATTTGA GGAATCAGAA CGCAAGCAAA 
     3901 ATGCGTTAGG AAAATGTGAT GCATGTCTCA GTGACAAAGC ACAGTTGCTT CTAGACCAAG 
     3961 AGGCTGAATC AGCATCCCAA AAGATAGAAG ATGGTAAAAC CCCTAAGCCA CTTTCTCTGA 
     4021 AATCTGATAG GTCAACAAAC AATGTGGAGA GGCATACTCC AAGGACCAAG ATTAGACCTG 
     4081 TAAGTTTGCC TGTAGATAGA CTACTTCTTG CAAGTCCTCC TAATGAGAGA AATGGCAGAA 
     4141 ATATGGGAAA TGTAAATTTA GACAAGTTTT GCAAGAATCC TGCCTTTGAA GGAGTTAATA 
     4201 GAAAAGACGC TGCTACTACT GTTTGTTCCA AATTTAATGG CTTTGACCAG CAAACTCTAC 
     4261 AGAAAATTCA GGACAAACAG TATGAACAAA ACAGCCTAAC TGCCAAGACT ACAATGATCA 
     4321 TGCCCAGTGC ACTCCAGGAA AAAGGAGTGA CAACAAGCCT CCAGATTAGT GGGGACCATT 
     4381 CTATCAATGC CACTCAACCC AGTAAGCCAT ATGCAGAGCC AGTCAGGTCA GTGAGAGAGG 
     4441 CATCTGAGAG ACGGTCTTCA GATTCCTACC CTCTCGCTCC TGTCAGAGCA CCCAGAACAC 
     4501 TGCAGCCTCA ACATTGGACA ACATTTTATA AACCACATGC TCCCATCATC AGTATCAGGG 
     4561 GGAATGAGGA GAAGCCAGCT TCACCCTCAG CAGCAGTGCC TCCTGGCACA GATCACGATC 
     4621 CCCACGGTCT CGTGGTGAAG TCAATGCCAG ACCCAGACAA AGCATCAGCT TGTCCTGGGC 
     4681 AAGCAACTGG TCAACCTAAA GAAGACTCTG AGGAGCTTGG CTTGCCTGAT GTGAATCCAA 
     4741 TGTGTCAGAG ACCAAGGCTA AAACGAATGC AACAGTTTGA AGACCTCGAA GATGAAATTC 
     4801 CACAATTTGT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4861 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4921 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4981 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5041 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5101 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5161 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5221 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5281 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5341 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5401 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5461 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5521 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5581 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5641 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5701 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5761 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5821 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5881 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5941 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6001 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6061 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6121 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6181 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6241 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6301 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6361 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6421 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6481 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6541 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6601 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6661 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6721 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6781 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6841 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6901 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6961 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7021 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7081 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7141 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7201 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7261 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7321 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7381 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7441 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7501 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7561 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7621 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7681 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7741 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7801 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7861 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7921 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7981 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8041 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8101 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8161 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8221 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8281 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8341 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8401 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8461 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8521 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8581 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8641 TAACAAAAAT TTAACGCGAA TTTT
//